AgraStrip Semi-Quantitative Low-LOD Corn Comb
|
|
- Horatio Parks
- 5 years ago
- Views:
Transcription
1 SEMI-QUANTITATIVE Low-LOD CORN COMB BULK GRAIN AgraStrip Semi-Quantitative Low-LOD Corn Comb Order Number: Semi-Quantitative Procedure to Detect CP4 EPSPS, Cry1, Cry3Bb, Cry1F, PAT, Cry34Ab1 and/or VIP3A Proteins in Corn Grain Using Multi-Trait Combs Application Description Transgenic products have been developed for insect-resistant corn including YieldGard, YieldGard Rootworm, Genuity, Herculex I, and Herculex RW brands, and herbicide-resistant corn including the Roundup Ready brand and LibertyLink. The intended use of this application is the semi-quantitative determination of transgenic protein(s) in corn grain samples using a single 800-kernel sample extracted with water. This application is based on the USDA GIPSA Directive which recommends 2400 kernel sub-samples be screened for qualitative detection (e.g. 3 sub-samples of 800 kernels). This application can screen for Genuity VT Triple Pro (CP4 EPSPS, Cry1A.105, Cry3Bb1), Herculex I (Cry1F), LibertyLink (PAT), or Herculex RW/XTRA, (Cry34Ab1) corn grain at specified levels. The strip(s) will also detect YieldGard (Cry1Ab), YieldGard Rootworm (Cry3Bb1), and Roundup Ready (CP4 EPSPS). Contents of Kit Quantity AgraStrip SemiQuantitative Corn Comb Bulk Grain Strip Test- Trait 20 combs Sample Cups 20 Transfer pipettes (COOLS2010) 20 Note: The AgraStrip Semi-Quantitative Corn Comb Bulk Grain Strip Test Trait kit contains 7 test strips to detect the following transgenic proteins: Protein Detected CP4 EPSPS (blue strip) Cry1A.105) (green strip) Cry3Bb (silver strip) Cry1F (orange strip) PAT (purple strip) Cry34Ab1 (black strip) VIP3A (red strip on end) GMO Variety Genuity VT Triple Pro (MON88017 x MON89034) Genuity VT Triple Pro (MON88017 x MON89034) Genuity VT Triple Pro (MON88017 x MON89034) Herculex I TC1507 LibertyLink T25 Herculex RW DAS Detection Level # GM Kernels in % 1 0.5% % % 2 0.5% 4 0.1% 1 AgriSure Viptera TM % 1 Finalized 25 April
2 Materials Required but not Supplied: SemiQuantitative GMO Buffer Concentrate ( , makes 1L; , makes 4L, or , makes 20L). 1L Nalgene bottle, for use with (COSST5080) 4L Cubitainer, for use with (COSST5081) 20L Cubitainer, for use with (COSST5096) Timer (EQOLE1301) Graduated Cylinder, 50mL (EQOLE1051) Balance, 400g (EQOLE1010) Blender (110V: EQOLE1020; 220V: EQOLE1027) Blender blade with gasket set (EQOLE1026) AgraVision Reader (EQASR1007) and GMO Comb Tray (EQASR1033) Storage and Preparation of Reagents The AgraStrip Semi-Quantitative Corn Comb Bulk Grain Strip Test - Trait can be stored at room temperature or refrigerated (2-25ºC). Do not freeze. Once opened, the AgraStrip Semi-Quantitative Corn Comb Bulk Grain Strip Test Bulk Grain - Trait lateral flow test strips must be stored in the closed foil bag with desiccant. The desiccant must be blue in color. If the desiccant is pink, contact Romer Labs, Inc. Technical Service. Storage conditions higher than room temperature may adversely affect performance. Preparation of Semi-Quantitative Extraction Buffer: The Semi-Quantitative extraction buffer should be prepared according to the instructions on the bottle. For , the contents of the bottle are added to 1L of water in a 1L Nalgene bottle. For , the contents of the bottle are added to 4L of water in a 4L Cubitainer. For , the contents of the bottle are added to 20L of water in a 20L Cubitainer. Sampling The samples used for the AgraStrip Semi-Quantitative Corn Comb Bulk Grain Strip Test - Trait can be sub-samples of representative samples collected following GIPSA sampling procedures from trucks, railcars, barges, etc. for other tests. The size of the sub-samples to be used for the AgraStrip Semi-Quantitative Corn Comb Bulk Grain Strip Test - Trait will depend on the percent GMO screening level desired and an acceptable level of risk that the GMO level is close to the screening level. Note: It is assumed that the samples collected are representative of the contents of the truck or container and are sufficiently mixed to contain a random distribution of the sample contents. Sample Preparation The corn sample is ground and then extracted with Semi-Quantitative GMO Buffer in a polypropylene Mason -type jar. The sample preparation is important for the proper function of the test, especially the ratio of buffer to the weight of the corn sample. The volume of buffer in milliliters should be 2.0 times the weight of the corn sample in grams (g). Corn: Sample Weight (g) x 2.0 = Buffer Volume (ml) Finalized 25 April
3 The size of the Mason jar required and the grinding time depends on the sample size to be analyzed. For 800-kernel samples, a 32-oz. Mason jar is used. The processing parameters were determined using the laboratory grade Waring Model 31BL91 food processor with the standard blade. Other food processors may require different parameters. Sample Processing 1. Weigh sub-samples from each truck or container. 2. Place each sub-sample in a clean, dry polypropylene Mason jar of the appropriate size. See Table B. 3. Attach the jar adapter and clean, dry cutting blades. 4. Place the jar onto the food processor, place a shield over the jar if desired, and grind the sub-sample on high speed for the time indicated in Table B. Caution: If using glass Mason jars, it is recommended to shield the jars during grinding with a tri-cornered 1-liter plastic beaker. 5. Remove the adapter and cutting blades. 6. Add the volume of semiquantitative GMO buffer (see below) to the ground corn in the jar, place a lid on the jar and shake the jar until all the ground corn is well wetted (about 15 sec.) Sample Weight (g) X 2.0 = Buffer Volume (ml) Note: The sample will have a thick consistency but should contain some free liquid after a short settling time. There should be no whole kernels remaining. 7. Use this free liquid as sample in the Test Procedure. Test Procedure 1. Transfer ~10mL of the liquid from each sample prepared above into separate sample cups. Note: This is approximately ¼ fill volume of the sample cup. 2. For each sub-samle, place one AgraStrip Semi-quantitative Corn Comb - Trait Test Comb into the sample cup. Note: The sample liquid level in the cup should be between the arrows and the strip name on the filter cover. 3. Let the strip comb sit for 5 minutes. 4. The appearance of one line (control) on the strip indicates a negative result. 5. The appearance of two lines or more on the strip indicates a positive result. 6. Cut off the colored section at the bottom of each strip on the comb. 7. Insert the strips from the comb into the AgraVision Reader. A maximum of 4 strips can be read at once. The comb must be cut in half on the dotted line (between the Cry1F and LL strips) to allow reading in the AgraVision reader. Note that the right half (3 strips) includes a blank spacer on the card to aid in positioning in the tray. 8. Select the respective methods for each half of the comb from the menu on the AgraVision reader. These are listed as Low LOD Corn Comb 1 of 2 and Low LOD Corn Comb 2 of 2. Each method is for the respectively-labeled section of the comb (RUR strip starts 1 of 2; LL strip starts 2 of 2). 9. Results will appear in semi-quantitative ranges (i.e. <0.1%, %, %, %, >5%). Finalized 25 April
4 Interpreting the Lateral Flow Strip Test Check the result window at five (5) minutes after inserting the strip. At least one line, the Control Line, should always develop approximately one (1) cm down from the Reservoir Pad. A red line in this position indicates that the device is functioning properly. A red line appearing below the Control Line is the Test Line and indicates a positive result. If the test strip displays two (2) red lines, the test is complete and the sample is positive for GMO corn. If at 5 minutes the test strip only shows a clearly visible Control Line, then the sample is negative for GMO corn. Use the AgraVision Reader to Semi-Quantitatively interpret the results. Note: Test strip results should be interpreted after 5 minutes. Test strips interpreted after 10 minutes are invalid. Limit of Detection and Quantitation Range: Strip LOD Range of Test CP4 EPSPS 0.1% % Bt-Cry1A % % Bt-Cry3Bb1 0.25% % Bt-Cry1F 0.25% % PAT 0.5% % Bt-Cry34Ab1 0.1% % VIP3A 0.1% % Illustration of Lateral Flow Strips Cut Here Finalized 25 April
5 Example of an unreacted, negative (1- line) and positive (2-lines) test strip Equipment Cleaning and Drying Caution: It is important to clean and dry the jars and cutting blades between samples. 1. The Mason jar should be emptied, rinsed thoroughly with water and completely dried with a paper towel between uses. 2. The cutting blades for the blender should be rinsed with water until all ground corn is removed, washed using standard household liquid soap, rinsed well and carefully dried. If available, spraying or rinsing with methanol or isopropyl (rubbing) alcohol will assist drying. Finalized 25 April
6 Exclusion of Warranty Statement The user assumes all risk in using Romer Labs, Inc. products and services. Romer Labs, Inc. will warrant that its products and services meet all quality control standards set by Romer Labs, Inc., and Romer Labs, Inc. will, at its option, repair or replace any product, components, or repeat services which prove to be defective in workmanship or material within product specific warranty periods or expiration dates and which our examination shall disclose to our satisfaction to be defective as such. This warranty is expressly in lieu of all other warranties, expressed or implied, as to description, quality, merchantability, fitness for any particular purpose, productiveness, or any other matter. Romer Labs, Inc. shall be in no way responsible for the proper use of its products. Romer Labs, Inc. hereby disclaims all other remedies, warranties, guarantees or liabilities, expressed or implied, arising by law or otherwise, and it shall have no liability for any lost profits or damage, direct, indirect or otherwise, to person or property, in connection with the use of any of its products or services. This warranty shall not be extended, altered or varied except by a written instrument signed by an authorized representative of Romer Labs, Inc. For Technical Service Call: Romer Labs, Inc Stylemaster Dr. Union, MO Phone: (636) Fax: (636) Finalized 25 April
CORN, CANOLA, & ALFALFA BULK GRAIN
CORN, CANOLA, & ALFALFA BULK GRAIN AgraStrip RUR-HS Bulk Grain Strip Test Part Number 7000011 Intended Use The intended use of the kit is the qualitative (yes/no) determination of the CP4 EPSPS protein
More informationQuickStix Kit for. Roundup Ready Bulk Soybeans. Intended Use. How the Test Works. Sample Preparation. Catalog Number AQ 010 BGB
QuickStix Kit for Roundup Ready Bulk Soybeans Highlights: Results in 5 minutes or less Both bulk soybean kits utilize Common Extraction : - Roundup Ready - LibertyLink Available as 100-strip kit, in bulk
More informationLibertyLink Bulk Soybeans
QuickStix Kit for LibertyLink Bulk Soybeans Catalog Number AQ 014 BGB Intended Use This EnviroLogix QuickStix Kit for QuickScan - LibertyLink Bulk Soybeans is designed to extract and detect the presence
More informationRoundup Ready CP4 EPSPS PathoScreen kit for the detection of CP4 EPSPS protein Peroxidase Label Catalog number: PSP 74000
List of contents Roundup Ready CP4 EPSPS Lot Number Item 96 wells 288 wells 480 wells 4800 wells Antibody-coated 96-well microtiter plates 1 plate 3 plates 5 plates 50 plates Peroxidase enzyme conjugate
More informationCoffee Burr Grinder. Model #559. Instructions. Warranty
Coffee Burr Grinder Model #559 Instructions Warranty IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including the following: 1. Read all instructions.
More informationNew Insect Traits in Corn. Glenn Studebaker Extension Entomologist
New Insect Traits in Corn Glenn Studebaker Extension Entomologist Insect Pests of Arkansas Corn Soil Insects Wireworms White Grubs Seed Corn Maggot Southern Corn Rootworm Fire Ants Seedling Insects Cutworms
More informationBBQ Grill. Owner s Manual Save These Instructions - For Household Use Only For Sizes: 12 x 18 & 12 x 12
BBQ Grill Owner s Manual Save These Instructions - For Household Use Only For Sizes: 12 x 18 & 12 x 12 Do not use the Copper Chef TM BBQ Grill until you have read this manual thoroughly. Warranty Information
More informationTD4, TD4T ICED TEA/COFFEE DISPENSERS
TD4, TD4T ICED TEA/COFFEE DISPENSERS INTRODUCTION USE & CARE INSTRUCTIONS These dispensers are designed to hold and serve up to 4-gallons of iced tea or iced coffee. 10334.0000K 06/09 1998 Bunn-O-Matic
More informationFast Brew 12-Cup Coffeemaker
READ AND SAVE THESE INSTRUCTIONS WA R N I N G : A risk of fire and electrical shock exists in all electrical appliances and may cause personal injury or death. Please follow all safety instructions. Fast
More information12 CUP COFFEE MAKER User Guide
BRAND 12 CUP COFFEE MAKER User Guide Item: 740553 Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Ginnys.com/Blog Welcome to the world of full-flavored coffee with the Ginny
More information12 CUP COFFEEMAKER User Guide
BRAND 12 CUP COFFEEMAKER User Guide Item: 757147 Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Welcome to the world of full-flavored coffee with the Ginny s Brand 12-Cup Coffeemaker!
More informationWi-Fi Coffee Maker. Model# GCMW-4750 USER MANUAL
Wi-Fi Coffee Maker Model# GCMW-4750 USER MANUAL Read this manual thoroughly before using and save it for future reference Model: GCMW-4750 2018 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY
More informationTHE COBURN COMPANY, INC. PO Box 147 Whitewater, WI U.S.A. Phone: Fax:
THE COBURN COMPANY, INC. PO Box 147 Whitewater, WI 53190-0147 U.S.A. Phone: 262-473-2822 Fax: 262-473-3522 800-776-7042 800-776-7044 www.coburnco.com Instruction Manual - Milky Butter Churns Coburn part
More informationHand Mixer. In U.S.A
Hand Mixer In U.S.A. -800-85-8900 840074700 Mixer Safety IMPORTANT SAFEGUARDS When using electric appliances, basic safety precautions should always be followed, including the following:. Read all instructions.
More informationDISCONTINUED VERSION The information in this manual is no longer current. Espresso/Cappuccino OPERATING & SERVICE MANUAL BUNN-O-MATIC CORPORATION
BUNNEspress BUNN Espress DISCONTINUED VERSION The information in this manual is no longer current. Espresso/Cappuccino OPERATING & SERVICE MANUAL BUNN-O-MATIC CORPORATION POST OFFICE BOX 3227 SPRINGFIELD,
More informationUser Manual. Thermo Pump System Coffee Brewers User Manual. Models: 177C10, 177C15 04/2018. Please read and keep these instructions. Indoor use only.
Thermo Pump System Coffee Brewers User Manual Models: 177C10, 177C15 04/2018 Please read and keep these instructions. Indoor use only. www.avantcoequipment.com 1 Parts Diagram 177C15 2 5 177C10 3 4 6 4
More informationSingle-Serve Coffee Maker
840251801 Single-Serve Coffee Maker IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed to reduce the risk of fire, electric shock, and/or injury to
More informationK35 Use & Care Guide. Get the most from your new Keurig coffee maker
K35 Use & Care Guide Get the most from your new Keurig coffee maker K35 COFFEE MAKER A. Water Reservoir Lid B. MAX Fill Line C. Water Reservoir D. Water Reservoir Lock Tab Slots E. Power Cord F. Handle
More informationphd Pitcher UltraWater Owner s Manual Delicious Great Tasting Antioxidant Alkaline Energized
Owner s Manual UltraWater phd Pitcher Delicious Great Tasting Antioxidant Alkaline Energized READ ME FIRST! 1. Be sure to flush your UltraWater filter before using the first time. (See Instructions for
More informationFIVE SPEED HAND MIXER User Guide
BRAND FIVE SPEED HAND MIXER User Guide Item: 740782 Recipes Inside! Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Ginnys.com/Blog Thank you for purchasing a Ginny s Brand Five
More information2012 Guide to Bt Corn for Georgia
2012 Guide to Bt Corn for Georgia G. David Buntin, Professor and Grain Crop Entomologist Updated December 12, 2011 What is Bt corn? Bt corn is defined as corn hybrids that contain one or more gene(s) from
More informationBurr Grinder. Model #551. Instructions. Warranty
Burr Grinder Model #551 Instructions Warranty IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including the following: 1. Read all instructions.
More informationElectric Two-Speed Drink Mixer
Electric Two-Speed Drink Mixer MS2090 Series Before use, please read this manual thoroughly and save for future reference. ovente.com Electric Two-Speed Drink Mixer MS2090 Series 2 ovente.com ELECTRIC
More informationQuick Start Guide Read this booklet thoroughly and save these instructions.
Quick Start Guide Read this booklet thoroughly and save these instructions. FAQs can be found at www.espresso-works.com Email us for any unanswered questions: cs@espresso-works.com For product registration,
More informationWE CAN HELP. Smart Coffee Maker User Manual. Can t connect? Need help? DO NOT RETURN THIS PRODUCT TO THE STORE
Can t connect? Need help? Wi-Fi Date Code: 01/19 Smart Coffee Maker User Manual WE CAN HELP DO NOT RETURN THIS PRODUCT TO THE STORE Call 1-800-757-1440 Mon-Fri 9:00-5:00 EST (US) or email info@atomiusa.com
More informationPECTINASE Product Code: P129
PECTINASE Product Code: P129 Enzyme for sample clarification prior to patulin analysis. For in vitro use only. P129/V1/02.06.16 www.r-biopharm.com Contents Page Test Principle... 3 Kit Components... 3
More informationSPUD MASHER User Guide
BRAND SPUD MASHER User Guide Item: 742487 Recipes Inside! Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Ginnys.com/Blog Thank you for purchasing a Ginny s Brand Spud Masher.
More informationSPECIAL BREW COFFEE MAKER INSTRUCTION MANUAL MODEL: BVCLDCG1
SPECIAL BREW COFFEE MAKER INSTRUCTION MANUAL MODEL: BVCLDCG1 1 TABLE OF CONTENTS Important Safeguards 3 Polarized Plug 4 Product Diagram 5 Usage instructions 1. Preparing Your Special Brew Coffee Maker
More informationCoffee Burr Grinder. Model #591. Instructions. Warranty. 110W/120Vac/60 Hz. Read this manual thoroughly before using and save it for future reference
Coffee Burr Grinder Model #591 Instructions Warranty Read this manual thoroughly before using and save it for future reference 110W/120Vac/60 Hz IMPORTANT SAFEGUARDS When using electrical appliances, basic
More informationProgrammable Conical Burr Mill CBM-18
Programmable Conical Burr Mill CBM-18 IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including the following: 1. Read all instructions. 2. To
More informationCoffeemaker. Coffeemaker Safety... 2 Parts and Features... 3 Using Your Coffeemaker... 4 Cleaning Your Coffeemaker... 4 Customer Service...
Coffeemaker Coffeemaker Safety... 2 Parts and Features... 3 Using Your Coffeemaker... 4 Cleaning Your Coffeemaker... 4 Customer Service... 6 In USA: 1-800-851-8900 In Canada: 1-800-267-2826 840069400 Coffeemaker
More informationK Mini Plus. Use & Care Guide. Get the most from your new Keurig brewer
K Mini Plus Use & Care Guide Get the most from your new Keurig brewer STRONG STRONG Before You Brew 1 Plug & Power 2 Fill & Place Remove packing tape from brewer. Pull plug and extend cord from cord storage
More informationUSE AND CARE GUIDE FOR
READ AND SAVE THESE INSTRUCTIONS Coffee Maker USE AND CARE GUIDE FOR MODEL ME2TMB IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including the
More informationCOLD BREW COFFEE MAKER. Model# GCM-7800 USER MANUAL
COLD BREW COFFEE MAKER Model# GCM-7800 USER MANUAL Read this manual thoroughly before using and save it for future reference FOR CUSTOMER SERVICE QUESTIONS OR COMMENTS VISIT US @ GOURMIA.COM INFO@GOURMIA.COM
More informationTriple Brew. Model #352. Coffee & Tea Maker. Instructions Warranty
Triple Brew Coffee & Tea Maker Model #352 Instructions Warranty IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including: 1. Read all instructions.
More informationBRAND DOUBLE WAFFLE MAKER
BRAND DOUBLE WAFFLE MAKER User Guide Item: 759106 Recipes Inside! Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Thank you for purchasing a Ginny s Brand Double Waffle Maker.
More informationGravimetric Analysis
Experiment 1: Gravimetric Analysis with Calcium Chloride and Potassium Carbonate In this experiment, proper analytical experimental techniques will be utilized to perform a double displacement reaction.
More information5 Piece Bakeware Set 1 YEAR LIMITED WARRANTY
1 YEAR LIMITED WARRANTY Course Housewares, LLC warrants this Cook s Companion Bakeware product to be free from manufacturer defect for up to 1 year from the original date of purchase. If a defect is found
More informationMODEL# GMF255 MOKA POT MILK FROTHER. PHOTO OF PRODUCT
MODEL# GMF255 MOKA POT MILK FROTHER www.gourmia.com PHOTO OF PRODUCT 2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY Welcome to the World of Espresso & Milk Frothers from Gourmia! All rights
More informationTF SERVER (With & Without Base)
1.0 & 1. Gallon TF SERVER (With & Without Base) DISCONTINUED VERSION The information in this manual is no longer current. USE & CARE INFORMATION CAUTION: CLEAN AND SANITIZE YOUR SERVER BEFORE USING. SEE
More informationSG120 Model # Cup Stainless Steel Coffee Maker. Operating Instructions Product Registration Warranty
SG120 Model #494 12-Cup Stainless Steel Coffee Maker Operating Instructions Product Registration Warranty IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be
More informationMODEL# GCM4500 COFFEE MAKER WITH GRINDER. PHOTO OF PRODUCT
MODEL# GCM4500 COFFEE MAKER WITH GRINDER www.gourmia.com PHOTO OF PRODUCT 2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY Welcome to Delicious and Aromatic world of Coffee Makers from Gourmia!
More informationPAGE TITLE. Instruction manual MODEL DPBD002
PAGE TITLE Instruction manual MODEL DPBD002 TABLE OF CONTENTS 2 TABLE OF CONTENTS Important Safeguards... 4-5 Parts & Features... 6-7 Using Your Blender... 8-9 Maintenance... 10-11 Recipes... 12-17 Customer
More informationModel # Instructions Warranty. 950 Watts / 120 Vac / 60 Hz. Read this manual thoroughly before using and save it for future reference
Perk Model #405.05 Instructions Warranty 950 Watts / 120 Vac / 60 Hz Read this manual thoroughly before using and save it for future reference IMPORTANT SAFEGUARDS When using electrical appliances, basic
More informationAgraStrip Allergens - Lateral Flow Devices
AgraStrip Allergens - Lateral Flow Devices The AgraStrip Allergen Test Kits are immunological rapid test in lateral flow format for the detection of allergens in food, rinse waters and environmental swabs.
More informationDouble Coffeemaker Instruction Manual
BRAND Double Coffeemaker Instruction Manual Item 710431 Ginnys.com 1.800.544.1590 Thank you for purchasing a Ginny s Brand Double Coffeemaker. With two 12-cup carafes, you ll easily keep enough piping-hot
More informationBooklet_TCG-357_ENG.qxd 9/8/08 11:19 AM Page 1 COFFEE/SPICE GRINDER. Instruction Booklet. Model: TCG-357
Booklet_TCG-357_ENG.qxd 9/8/08 11:19 AM Page 1 COFFEE/SPICE GRINDER Instruction Booklet Model: TCG-357 Booklet_TCG-357_ENG.qxd 9/8/08 11:19 AM Page 2 IMPORTANT SAFEGUARDS When using electrical appliances,
More informationElectric Griddle. Griddle Safety... 2 Parts & Features... 3 How to Use Your Griddle... 3 Cleaning Your Griddle... 4 Recipes... 5 Customer Service...
Electric Griddle 840098400 Griddle Safety... 2 Parts & Features... 3 How to Use Your Griddle... 3 Cleaning Your Griddle... 4 Recipes... 5 Customer Service... 8 In USA: -800-85-8900 In Canada: -800-267-2826
More informationUSE & CARE INSTRUCTIONS ESPRESSO MAKER FES4B
USE & CARE INSTRUCTIONS ESPRESSO MAKER FES4B IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed, including the following: 1. Read all instructions.
More informationMINI MAKER GRILL. Model DMG001. Instruction Manual & Recipe Guide
MINI MAKER GRILL Instruction Manual & Recipe Guide Model DMG001 TABLE OF CONTENTS MINI MAKER GRILL Important Safeguards...4-5 Parts & Features...6 Using Your Grill...7-9 Maintenance & Cleaning...10 Troubleshooting...
More informationUse & Care. All about the. of your Coffee Maker TA B L E O F C O N T E N T S. Brewing Your First Pot of Coffee USA and Canada
All about the Use & Care of your Coffee Maker TA B L E O F C O N T E N T S Important Safeguards...2 Brewing Tips... 8 Coffee Maker Safety Instructions... 2 Cleaning the Coffee Maker... 8 Parts and Features...3
More informationInstruction Booklet Wine Kit FPSTBW8055 MODEL FPSTBW , P.N.:
Instruction Booklet Wine Kit MODEL FPSTBW8050-033, FPSTBW8055 www.oster.ca P.N.: 143061 IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed including
More informationTitle: Reagents Copy No: ## SOP No.: 3.13/3.1/S Effective Date: July 8, 2013 Location: ### Reagents
QSM Approval: Reagents 1. Introduction Laboratory reagents are used in all procedural steps during the course of sample preparation. To ensure that reagents are of known quality and purity and free of
More informationMODEL# GCG165 COFFEE GRINDER. PHOTO OF PRODUCT
MODEL# GCG165 COFFEE GRINDER www.gourmia.com PHOTO OF PRODUCT 2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY Welcome to Delicious world of Coffee Grinders from Gourmia! All rights reserved.
More informationROTISSERIE MINI OVEN KWS1523X-F2UB
ROTISSERIE MINI OVEN KWS1523X-F2UB CAUTION Read all the instructions carefully before using and retain this for further reference. The temperature of accessible surfaces may get hot when the appliance
More information6 oz SHOT. Use & Care Guide. Get the most from your new Keurig brewer
6 Use & Care Guide Get the most from your new Keurig brewer 6 6 6 6 Before You Brew 1 Plug & Place Rinse & Fill 2 Remove packing tape from brewer and frother, and plug into a grounded outlet. Lower the
More informationFood Allergen and Adulteration Test Kits
Food Allergen and Adulteration Test Kits Overview Neogen offers food allergen test kits to detect almond, egg, gliadin, hazelnut, milk, mustard, peanut, sesame, shellfish, soy and walnut residues (see
More informationLet s get brewing. K Quick Start Guide
Let s get brewing. K-1500 Quick Start Guide IMPORTANT SAFEGUARDS - READ ALL INSTRUCTIONS BEFORE USING When using electrical appliances, basic safety precautions should be followed, including the following:
More informationCafeRomatica NICR7.. Fully automatic coffee centre Operating Instructions and Useful Tips. A passion for coffee.
CafeRomatica Fully automatic coffee centre Operating Instructions and Useful Tips NICR7.. GB A passion for coffee. 1 G F A M J / K A B C D E Display screen Left rotary knob Right rotary knob Bean symbol
More information1. Blender: Osterizer, 10-speed, or equivalent. 2. Separatory Funnel: Kilborn or equivalent (see figure 1) 2. HCl Solution: HCl/water (7:93 by volume)
EXTER.01-1 INFESTATION IN WHOLE CORN PRINCIPLE Whole corn is suspended in aqueous borax solution to float insects and insect fragments, which are collected on filter paper for microscopic identification
More informationUSE AND CARE OF YOUR DELUXE. Coffee Grinder CONTAINS A SECTION ON COFFEE FUNDAMENTALS. Keep this booklet for future reference
USE AND CARE OF YOUR DELUXE Coffee Grinder CONTAINS A SECTION ON COFFEE FUNDAMENTALS Keep this booklet for future reference WELCOME Welcome to the world of fine coffee preparation. The Bunn Deluxe Home
More informationAUTOMATIC BURR COFFEE GRINDER
AUTOMATIC BURR COFFEE GRINDER MODEL:SCG-903B USER MANUAL www.thesecura.com Welcome Congratulations on purchasing our Secura Automatic Burr Coffee Grinder. Each unit is manufactured to ensure safety and
More informationCertified Home Brewer Program. Minimum Certification Requirements
Certified Home Brewer Program Minimum Certification Requirements SCA's Minimum Certification Requirements for Coffee Brewers 1. Coffee Volume: The volume of the brew basket must be sized in proportion
More informationEP-AERATOR001 OWNER S MANUAL
Trilux Wine Aerator EP-AERATOR001 OWNER S MANUAL EPICUREANIST TRILUX WINE AERATOR Thank you for purchasing an EPICUREANIST product. Please read all the instructions before attempting to operate this product
More informationUser Manual. Stainless Steel Coffee Urns. Models: 177CU55ETL, 177CU110ETL 10/2016. Please read and keep these instructions. Indoor use only.
Intertek Stainless Steel Coffee Urns Models: 177CU55ETL, 177CU110ETL 10/2016 Please read and keep these instructions. Indoor use only. www.avantcoequipment.com 1 NOTE: Save these instructions for future
More information2016 Gourmia The Steelstone Group Brooklyn, NY
2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY All rights reserved. No portion of this manual may be reproduced by any means whatsoever without written permission from The Steelstone Group,
More informationProaster. 1 Year Limited Warranty. Patent Pending. Patent Pending
1 Year Limited Warranty Course Housewares, LLC warrants this Cook s Companion cookware product to be free from manufacturer defect for up to 1 year from the original date of purchase. If a defect is found
More informationINSTRUCTION MANUAL AND RECIPE BOOK CORN DOG MAKER CDM-1 SMART PLANET PLEASE READ THESE INSTRUCTIONS CAREFULLY AND RETAIN FOR FUTURE REFERENCE.
INSTRUCTION MANUAL AND RECIPE BOOK CORN DOG MAKER CDM-1 SMART PLANET PLEASE READ THESE INSTRUCTIONS CAREFULLY AND RETAIN FOR FUTURE REFERENCE. IMPORTANT SAFEGUARDS Read all instructions before using the
More informationWARRANTY. Stainless Steel Steam Tray
WARRANTY LIMITED WARRANTY Aroma Housewares Company warrants this product free from defects in material and workmanship for one year from provable date of purchase in the United States. Within this warranty
More informationIMPORTANT SAFEGUARDS SAVE THESE INSTRUCTIONS
Stand Mixer Important Safeguards... 2 Know Your Mixer... Using Your Mixer... 5 Cleaning Your Mixer...6 Recipes... 7-9 Customer Service... 0 In USA: -800-85-8900 In Canada: -800-267-2826 84007200 IMPORTANT
More informationTHE PANCAKE FACTORY. Model: PM-2
THE PANCAKE FACTORY Model: PM-2 IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed including the following: 1. Read all instructions. 2. Do not touch
More informationMODELS ME8TPB, ME8TPW, ME8TPBS, ME8TSW, ME8TSB AND ME8TPWCAN
READ AND SAVE THESE INSTRUCTIONS WARNING: A risk of fire and electrical shock exists in all electrical appliances and may cause personal injury or death. Please follow all safety instructions. 8 Cup Thermal
More informationinfinity Model # 575 #570 Conical Burr Grinder Instructions Warranty Product Registration 100W / 120V~ / 60 Hz
infinity Conical Burr Grinder Model # 575 #570 Instructions Warranty Product Registration 100W / 120V~ / 60 Hz IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always
More informationMR.BEER Hard Cider. You ll need the following:
Instructions These instructions will familiarize you with the HARD CIDER BREWING PROCEDURES of the MR.BEER Home Brewing System. The included recipe will produce about 2 gallons (8 quarts) of 3.0% alc/vol
More informationEach lab contains complete instructions there is no lab manual for this course. It is strongly
Physical Science Core Lab Materials Introduction Lab Options This course includes the option of hands-on or dry lab activities. Dry labs have no required materials. Hands-on labs require the materials
More informationK Café. Use & Care Guide. Get the most from your new Keurig brewer LATTE COLD CAPP
K Café Use & Care Guide Get the most from your new Keurig brewer AUTO OFF DESCALE ADD WATER 6 COFFEE & 8 0 oz oz oz 2oz SHOT Before You Brew Plug & Place 2 Rinse & Fill Remove packing tape from brewer
More informationBRAND. COPPER CERAMIC GRIDDLE GRILL User Guide. Recipes Inside! Item:
BRAND COPPER CERAMIC GRIDDLE GRILL User Guide Item: 753543 Recipes Inside! Ginnys.com 800-544-1590 Facebook.com/GinnysBrand Pinterest.com/GinnysBrand Thank you for purchasing a Ginny s Brand Copper Ceramic
More informationAutomatic Cold Brewer
Automatic Cold Brewer Model# GCM-6800 USER MANUAL Read this manual thoroughly before using and save it for future reference Model: GCM-6800 2017 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY
More informationG F I M J K L B C D E S O T H Q P U N R D 4
1 2 3 4 G F I A M J K L 5 6 7 B C D E 8 9 10 S O T H Q P U N R 11 12 D 4 Equipment GB A B C D E Text display (main menu) Rotary control knob Button > Button exit Height-adjustable coffee spout CafeRomatica
More informationSmart Valve Cold Brew Coffee Maker { Instruction Manual
Smart Valve Cold Brew Coffee Maker { Instruction Manual Table of Contents Important Safeguards... 2 Glass Decanter Safety Precautions... 3 Getting to Know Your Cold Brew Coffee Maker... 4 Easy-Grab Tab
More information6 QT. ALUMINUM LOW PRESSURE COOKER
USE & CARE 6 QT. ALUMINUM LOW PRESSURE COOKER YOUR SET INCLUDES: Viewing Window 2- Coat Enamel Exterior 1 Lid 2 Pot SAFE TO USE WITH: Induction Plate 1 Gordon Ramsay Low Pressure Cooking System The Gordon
More information2016 Gourmia The Steelstone Group Brooklyn, NY
2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY All rights reserved. No portion of this manual may be reproduced by any means whatsoever without written permission from The Steelstone Group,
More informationCocktail Master AUTOMATIC DRINK MIXER AND POURER
Cocktail Master AUTOMATIC DRINK MIXER AND POURER Table of contents Warnings and Cautions................................................... 2 Location of Parts and Controls............................................
More information1550W DIGITAL FRENCH DOOR OVEN Instruction Manual MODEL CCFD19
1550W DIGITAL FRENCH DOOR OVEN Instruction Manual MODEL CCFD19 1 WHAT S INCLUDED French Door Oven & Instruction Manual Rotisserie Hle Two Wire Racks Baking Pan Rotisserie Tray Hle Crumb Tray 2 IMPORTANT
More informationMG600 PLUS, Model #484 (Glass) 10-Cup Programmable Coffee Maker with Stainless Steel Housing. Operating Instructions Product Registration Warranty
MG600 PLUS, Model #484 (Glass) 10-Cup Programmable Coffee Maker with Stainless Steel Housing Operating Instructions Product Registration Warranty IMPORTANT SAFEGUARDS When using electrical appliances,
More informationDuothek Plus Model #464
Duothek Plus Model #464 Instructions for Use Warranty Guarantee of Performance IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed to reduce the risk
More informationsystemic houseplant insect control
systemic houseplant insect control Protects plants from damaging insects for up to 2 months Long lasting, effective protection For use in potted plants Protects plants from damage by: Aphids, Whiteflies,
More informationOWNERS MANUAL. My Rotisserie Pro Warranty. Please read and save this manual. PROFESSION AL SERIES MRP_MAN_QVC_ENG_V1_ FOR HOUSEHOLD USE ONLY.
My Rotisserie Pro Warranty The manufacturer warrants that your My Rotisserie Pro is free of defects in materials and workmanship and will, at its option, repair or replace any defective rotisserie that
More informationUser Manual. Stainless Steel Coffee Urns. Models: 177CU30, 177CU55, 177CU110 04/2018. Please read and keep these instructions. Indoor use only.
Stainless Steel Coffee Urns Models: 177CU30, 177CU55, 177CU110 04/2018 Please read and keep these instructions. Indoor use only. www.avantcoequipment.com 1 NOTE: Save these instructions for future reference.
More informationDick Hodgman s Recipes
Vanilla Cream Soda 8 oz. club soda 1 tbs. heavy cream ¼ tsp. vanilla extract 2 3 tsp. sugar Pour the club soda over ice in a glass. Mix in the cream and vanilla. Gently but thoroughly mix the sugar. Adjust
More information1. IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed to reduce the risk of fire, electric
1. IMPORTANT SAFEGUARDS When using electrical appliances, basic safety precautions should always be followed to reduce the risk of fire, electric shock, and/pr injury to persons including the following:
More informationrm1a. MODEL# GYM1710
rm1a. MODEL# GYM1710 Vo! Good Automatic Yogurt Maker 2016 Gourmia www.gourmia.com The Steelstone Group Brooklyn, NY All rights reserved. No portion of this manual may be reproduced by any means whatsoever
More information左边 :SAFTY NOTES 右边 : Please follow the safety precautions to avoid any harm to yourself and cause any damages Never Always Warning Attention
封面 Multi-functional Soymilk Maker Instruction Manual Please read all instruction carefully before operating the machine 第一页 Contents Safety notes 2 Usage notes 3 Product description and specification 4
More informationHand Crank Ice Shaver VKP1099
Hand Crank Ice Shaver VKP1099 Table of Contents Parts List & Diagram...1 Operating Precautions...2 Accessories...2 Use & Care Instructions...3 Creating Treats & Recipes...4 Warranty/Contact Info... Back
More information12-in-1 Kitchen Master Instruction Manual
BRAND 12-in-1 Kitchen Master Instruction Manual MODEL: HOUS-HS-3318 Ginnys.com 1.800.544.1590 Life is happening. We ll help you live it...one moment at a time. Thank you for purchasing the Ginny s Brand
More informationUser Manual. Stainless Steel Coffee Urn. Models: 177CU30 11/2018. Please read and keep these instructions. Indoor use only.
Stainless Steel Coffee Urn Models: 177CU30 11/2018 Please read and keep these instructions. Indoor use only. www.avantcoequipment.com 1 NOTE: Save these instructions for future reference. Index Important
More informationCoffee-and-Cream Science Jim Nelson
SCIENCE EXPERIMENTS ON FILE Revised Edition 5.11-1 Coffee-and-Cream Science Jim Nelson Topic Newton s law of cooling Time 1 hour! Safety Please click on the safety icon to view the safety precautions.
More informationcoffeemaker owners manual
840153800 v02.qxd 7/5/06 3:15 PM Page 1 coffeemaker owners manual SAVE THIS USE AND CARE BOOK Please call 1-800-851-8900 with questions 840153800 v02.qxd 7/5/06 3:15 PM Page 2 wwarning Shock Hazard Do
More informationKeurig Vue V600 Brewer Use & Care Guide
Keurig Vue V600 Brewer Use & Care Guide IMPORTANT SAFEGUARDS Safe Operation & Use When using electrical appliances, basic safety precautions should be followed, including the following: 1. Read all instructions
More informationWMR-CA. Classic Waffle Maker INSTRUCTION BOOKLET
INSTRUCTION BOOKLET Classic Waffle Maker WMR-CA For your safety and continued enjoyment of this product, always read the instruction book carefully before using. TABLE OF CONTENTS Important Safeguards..............
More information