Trichoderma. Supella longipalpa ( Dictyoptera Blattidae) Beauveria bassiana. 10x3 Trichoderma harzianum. T. harzianum. B. bassiana.

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1 harzianum Beauveria bassiana Trichoderma Supella longipalpa ( Dictyoptera : Blattidae) Beauveria bassiana 7 1x3 Trichoderma harzianum Supella longipalpa ( Dictyoptera Blattidae) T. harzianum B. bassiana B.C B. bassiana B. bassiana T. harzianum longipalpa Walker L Supella Blatta latralis Polyphaga aegyptica Blatella mella Kranss 4 Blattidae Periplaneta americana L Blattella germanica

2 Beauveria bassiana T. harzianum Who / VBC x , 1957,Willis Roth Johansso 1.5 x x 5 % 5±4 26± KOH T. harzianum 21 T. polsporum scolytus S. multistriatus Scolytus 199 Jassim B. Mc bassiana Mohan 1991 Guire T. B. bassiana Zukowski 1999 P.D.A harzianum Pachamuthu Agar , Dextrose 7 1 x 3 Haemocytometer.* Steenberg

3 P D.B. 7 1 x 3 8 Dextrose (Autoclave B. bassiana 1 T. harzianum 21 1 ±25 * (765) No.1 T. harzianum % 5% 1% 7 B.bassiana 25% 5% 1% % 1% 25% 1 7 7

4 Beauveria bassiana bassaina grasshopers B. C.R.D 1998) R.L.S.D 1993 Fehrenbach Metarhizium anisopliae B. germanica (1 ) % Graham - Bryce chitinase Beauverin 2 Jvanova Sukhoruchenko T. harzianum 22) Rhizopertha dominica 51.11% 26.67% 65.1 T. harzianum % ) 86.67% 1% T. harzianum P.D.A Lobolima 1993 Gottel Johnson 8

5 ( 1 ) B Bassiana T harzianum

6 Beauveria bassiana 2 1% 62.22% 25% 73.33% 3% T % 73.33% harzianum 1% 1% T. 25% harzianum 4% 1995 Beauverin 93.33% B.. bassiana chitinase %.Tetranychus urticae Koch 1972 Lappa Beauverin 21% Eurygastor integriceps 1993,Gottel 1988 Samson) Johnson 1997 chitinase Kramer 25%, 5%, 1% 6

7 النسبة المؤية للفقس % سلسلة القارنة B.bassiana T.harizianum %, 5%, 1% ( 2 ) % 5 5% 7 1% T. harzianum

8 Beauveria bassiana Dictyoptera Periplaneta americana ( L.) Dictyoptera : Blattidae Rhizopertha dominica F Coleoptera Bostrychidae Musca domestica L Tetranychus urticae k. Tetranychidae : Acari 65 Fehrenbach,P.C.1993.Biological warfore. Pest control technology. 21: Graham-Bryce,I.J.1997.Crop protection consideration of the effectivenss and disadvantages of current method and of thes corp for provement. Philophical Transacttion of the Royal society. London.series B 281:163. Jassim H.K. Foster,H.A Fairhust C.P Biological control of Dutch elm disease :larvicidal activity of Trichoderma harizanum, T. polysporum and Scytalidium lingnicola in Scolytus scolytus and S. multistriatus reared in artificial Musca domestica L Bemisia tabaci Cryptolestes ferrugineus Blattaria

9 cockroach (Periplaneta americana ).Biocontrol science and Technology.9 : Pachamuthu,P.,Kamble,ST.,Yyuen,GY.1999.Virulence of Metarhizium anisopliae (Deutetomycetes) strain ESC-1 to the German Cockroch (Dictyoptera: Blattidae ) and its compatility with Insecticides j. Eco. Ent. 92: Roth,L.M.& Willis,E.R.1957.The medical and veteinary importance of cockroaches. Smith sonianmise. Collect. 134 : Samson,AP.,Evans,C.and Latge,J Atlas of entomopathogenic fungi. Printsd in the Netherland,New York:187pp. Steenberg,T.,Vagn-Jensen, K.M., Jensen- K.M.V., Smitsph Entomopathogenic Fungi for control of German cockroach(blattella germanica)and othersynanthropic cockroaches. 21 : Sukhoruchenko,G. and Jvanova,G. 2. Pestresistance to pesticides and resistance Management in the former Soviet Union. J.A. Jachman, 63: culture. Ann. Appl. Biol.117: Johnson,D.L. and Gottel,M.S Reduction of the fungus Beauveria bassiana.biocontrol Science and Technology.3: Johansso, E Cockroaches as affod hygiene hazard. svensk, verinaertidning 48: Kramer, T Natural pest control.the North Coast. J. 1: Lappa, N. V.,Goral, VM.,Markys, V.G Action of Beauverin on eggs of Eurgaster integriceps Zakhyst. Kiev.16: Lobolima, M.L., Brito, J.M. and Henry, J.E Biological control of grasshpper in the capeverde islands. CAB international,u.k.394 pp Mc Guire,M.R.,streett, D.A. and shasha, B.S Evaluation of starch Encasulation For grasshopper (Orthoptera: Acarididae) entomo pox Viruses. J. Eco.Ent. 84: Mohan, C. M., Lakshmi, K. A., Devi, K. U Laboratory evaluation of the pathogeincity of three isolates of the entomopathogenic fungus Beauveria bassiana (Bals) Vuillemin on the American 9

10 Beauveria bassiana WHO Cockroaches Report of who\ VBC.4. Zukowski,K., Bajan, C., Popowska, N.Owak, E.1998.Evaluation of effect of Metarhizium anisopliae on reduction of numbers of Blattela germanica L.Roczniki-Panstwoweg- Zaklad-Higieny.49 :67-72.

12 Beauveria bassiana The laboratary effectivess of fungi Beauveria bassiana (the chines isolte ) and Trichoderma harzianum in Biological control of the brown banded cockroach Supella longipalpa ( Dictyoptera :Blattidae) Abdul-Hameed Y. Aylan Plant protection Dept. College of Agriculture,Basrah University, Basrah Iraq SUMMARY The research was conducted to study the efects of Beauveria bassiana (the chines isolte ) and Trichoderma harzianum in concentration of 3x1 7 Spore\ ml of each one )on the Brown babanded cockroach, Supella longipalpa ( Dictyoptera :Blattidae ). The resalt showed that the adult mortality which casued by when it used as baiting with wheat bran it about 86.67% with high significant. while the less percent 36.67% was obtained when the suspension of T. harzianum was used,with high significant difference with control treatment which has no adults mortality. The study also explained that reduced the hatching rate of eggs to 56.66% copared with control which was 93.33%. The exudates of fangi significanty increased the adults mortality rate. high concentration (1%) of exudates increased the adult mortality rate to1% and the low concentration ( 25% ) of T. harzianum led to increase the ratio to 4%, whil the control treatment has no mortality.

Evaluating the Efficiency of the Entomopathogenic Fungus Beauveria bassiana to Control the Brown Banded Cockroach Supella longipalpa F.

Evaluating the Efficiency of the Entomopathogenic Fungus Beauveria bassiana to Control the Brown Banded Cockroach Supella longipalpa F. Maan Abdul Azeez Shafeeq Al-Salihi Dept. of Biology/ College of Science/