ANTIOXIDANT ACTIVITY

Transcription

1 ANTIOXIDANT ACTIVITY The antioxidant activity of selected medicinal plants was performed at Chemistry Research Laboratory, Deogiri College, Aurangabad. 8.1 Preparation of sample solution About 0.10 g of dry plant powder was accurately weighed in a stopper tube. In stopper tube, 10.0 ml of aqueous methanol (40%) was added and incubated in a incubator for about 24 hrs. The contents of the tube were filtered through Whatman filter paper No. 41 (Merck, India). The filtrate was used for further analysis. 8.2 DPPH radical scavenging assay DPPH (2, 2-dipheny l-2- picrylhydrazyl) radical scavenging assay was carried out as per reported method with some modifications Blois (1958) and Roberta, et al., (2006). About 1 ml of test solution was added to equal quantity of 0.1 mm solution of DPPH in ethanol. After 20 min of incubation at room temperature, the DPPH reduction was measured by reading the absorbance at 517 nm. Ascorbic acid (1mM) was used as reference compound and it has ± 1.02 % of DPPH antioxidant assay. Sr. No Plant name DPPH antioxidant assay (%) 1 Cucumis setosus fruit ± Lagenaria siceraria fruit ± Trichosanthes tricuspidata fruit ± Coccinia grandis fruit ± Diplocyclos palmatus fruit ± Cucumis setosus leaf ± Trichosanthes tricuspidata leaf ± Coccinia grandis leaf ± Diplocyclos palmatus leaf ± Lagenaria siceraria leaf ± 0.73 Standard Ascorbic Acid ± 1.02 Table No 128: Antioxidant activity of selected medicinal plants 178

2 8.3.1 Antioxidant activity of Cucumis setosus fruit The antioxidant activity of methanol fruit extract of C. setosus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is ± Antioxidant activity of Lagenaria siceraria fruit The antioxidant activity of methanol fruit extract of Lagenaria siceraria was The DPPH antioxidant assay percentage is ± Antioxidant activity of Trichosanthes tricuspidata fruit The antioxidant activity of methanol fruit extract of Trichosanthes tricuspidata was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is ± Antioxidant activity of Coccinia grandis fruit The antioxidant activity of methanol fruit extract of Coccinia grandis was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is ± Antioxidant activity of Diplocyclos palmatus fruit The antioxidant activity of methanol fruit extract of Diplocyclos palmatus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is ± Antioxidant activity of Cucumis setosus leaf The antioxidant activity of methanol leaf extract of Cucumis setosus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is ±

3 8.3.7 Antioxidant activity of Trichosanthes tricuspidata leaf The antioxidant activity of methanol leaf extract of Trichosanthes tricuspidata was The DPPH antioxidant assay percentage is ± Antioxidant activity of Coccinia grandis leaf The antioxidant activity of methanol leaf extract of Coccinia grandis was The DPPH antioxidant assay percentage is ± Antioxidant activity of Diplocyclos palmatus leaf The antioxidant activity of methanol leaf extract of Diplocyclos palmatus was The DPPH antioxidant assay percentage is ± Antioxidant activity of Lagenaria siceraria leaf The antioxidant activity of methanol leaf extract of Lagenaria siceraria was The DPPH antioxidant assay percentage is ± 0.73 The DPPH free radical scavenging activity of standard reference ascorbic acid was ± In present study the DPPH free radical scavenging activity of fruit was more than that of reference standard ascorbic acid. Antioxidant analysis of present study reveals that the maximum DPPH antioxidant assay percentage was observed in Coccinia grandis fruit ± 0.52, Trichosanthes tricuspidata fruit ± 1.00, Diplocyclos palmatus fruit ± 0.25 and Cucumis setosus fruit ± 1.52 which was more than that of standard ascorbic acid. The Trichosanthes tricuspidata leaf has DPPH free radical scavenging activity ± 1.47 which also more than that of standard ascorbic acid. The results were dedicated in Table No 128 and Graph Nos 11& 12). 180

4 60 Plant Part Ascorbic Acid Cucumis setosus Trichosanthes tricuspidata Graph No: 11 Coccinia grandis Plant Part of leaf Diplocyclos palmatu Lagenaria siceraria Antioxidant activity of leaf of selected plants 181

5 Chapter 8: Antioxidant Activity Plant Part Ascorbic Acid Cucumis setosus Lagenaria siceraria Trichosanthes tricuspidata Plant Part of fruit Coccinia grandis Diplocyclos palmatus Graph No :12 Antioxidant activity of fruit of selected plants 182