Food Allergen and Adulteration Test Kits

Transcription

1 Food Allergen and Adulteration Test Kits Overview Neogen offers food allergen test kits to detect almond, egg, gliadin, hazelnut, milk, mustard, peanut, sesame, shellfish, soy and walnut residues (see page 7 for a description of how a sandwich ELISA works). Neogen s Alert for food allergen kits are screening tests that compare up to 5 at a time against a known level of allergen. The tests provide visible results that clearly show whether a sample contains more or less of a specific food allergen than the provided. Neogen s Reveal for food allergen kit is an easy-to-use and interpret strip test that screens for food allergen at 5 ppm in 10 minutes or less (see page 7 for a description of how lateral flow immunochromatic assays work). If the test detects at least 5 ppm, two lines will form on the test device. The formation of only one line indicates a negative test result. Neogen s for food allergen kits are quantitative tests that compare up to 19 at a time against 5 test s. Through the use of a microwell reader, the tests provide accurate sample results in parts per million. Neogen s Reveal 3-D tests for food allergens make it simple to screen for the presence of low levels of allergens in food products and environmental swabs virtually anywhere. Sample preparation and testing take less than 10 minutes. Neogen s BioKits Assays for food allergens are quantitative microwell tests that have been developed for use for the detection of allergens in foods. Almond Allergen Alert for Almond Allergen (screens at 5 ppm) Veratox for Almond Allergen (range ppm) Egg Allergen Alert for Egg Allergen (screens at 5 ppm) Veratox for Egg Allergen (range ppm) Gliadin Alert for Gliadin (screens at 10 ppm) Veratox for Gliadin (range 5 50 ppm) Hazelnut Allergen Veratox for Hazelnut Allergen (range ppm) Milk Allergen Alert for Total Milk Allergen (screens at 5 or 10 ppm) Reveal for Total Milk Allergen (screens at 5 ppm) Veratox for Total Milk Allergen (range ppm) Mustard Allergen Veratox for Mustard Allergen (range ppm) Peanut Allergen Alert for Peanut Allergen (screens at 5 ppm) Reveal for Peanut Allergen (screens at 5 ppm) Veratox for Peanut Allergen (range ppm) Soy Allergen Alert for Soy Allergen (screens at 10 ppm) Veratox for Soy Allergen (range ppm) Alert for Soy Flour Allergen (screens at 5 or 10 ppm) Veratox for Soy Flour Allergen (range ppm) BioKits for Allergens Reveal 3-D (Available for almond, casein, egg, gluten, hazelnut, peanut, shellfish and soy) BioKits Assays (Available for almond, BLG, casein, egg, gluten, hazelnut, peanut, sesame, shellfish, soya, walnut) DNA Allergen Selection (Mastermix pods for peanut, soy lecithin and beef [cows milk] and GMO & Allergen) BioKits for Food Adulteration Assays (Available for Pasta Quality Control [PQC] and Soya) Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

2 Alert For Almond Allergen For Almond Allergen Alert for Almond Allergen is used as a screening tool to test for almond residue in food products such as cookies, crackers, chocolate bars, ice cream, cereals and on environmental surfaces. Control provided: 5 ppm almond Tests per kit: Up to 20 Veratox for Almond Allergen is used for the quantitative analysis of almond residue in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm almond (-) sample (+) sample s 8441 Alert for Almond Allergen 8432S Allergen Environmental Swabbing Kit Veratox for Almond Allergen 48 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

3 Alert For Egg Allergen For Egg Allergen Alert for Egg Allergen is used as a screening tool to test for egg residue in food products such as pasta, salad dressing, cake mix and ice cream, and on environmental surfaces. Control provided: 5 ppm egg Tests per kit: Up to 20 Veratox for Egg Allergen is used for the quantitative analysis of egg residue in food products such as pasta, salad dressing, cake mix and ice cream. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm egg (-) sample (+) sample s 8451 Alert for Egg Allergen 8432S Allergen Environmental Swabbing Kit Veratox for Egg Allergen Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

4 Alert For Gliadin For Gliadin Alert for Gliadin is used as a screening tool to test for prolamins (wheat gliadin, rye secalin, and barley hordein) in ingredients, clean-in-place (CIP) solutions, finished food products and environmental surfaces intended to be prolamins (gluten) free. Control provided: 10 ppm gliadin (20 ppm gluten) Tests per kit: Up to 20 Veratox for Gliadin is used for the quantitative analysis of prolamins (wheat gliadin, rye secalin, and barley hordein) in ingredients, clean-in-place (CIP) solutions and finished food products intended to be prolamins (gluten) free. Range of quantitation: 5 ppm 50 ppm 0, 5, 10, 20 and 50 ppm gliadin (-) sample (+) sample s 8481 Alert for Gliadin 8432S Allergen Environmental Swabbing Kit Gliadin Extraction Additive for dark chocolate, cocoa and tannin 8483 Gliadin Cocktail Solution for heat processed ml 8483B Gliadin Cocktail Solution for heat processed ml 8480 Veratox for Gliadin 8482 Gliadin Extraction Additive for dark chocolate, cocoa and tannin 8483 Gliadin Cocktail Solution for heat processed ml 8483B Gliadin Cocktail Solution for heat processed ml 50 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

5 For Hazelnut Allergen Veratox for Hazelnut Allergen is used for the quantitative or qualitative analysis of hazelnut residues in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm hazelnut s 8420 Veratox for Hazelnut Allergen 8432S Allergen Environmental Swabbing Kit Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

6 Alert For Total Milk Allergen Reveal For Total Milk Allergen Alert for Total Milk Allergen is used as a screening tool to test raw ingredients, clean-in-place rinses, environmental surfaces, or product throughout the production process, for the presence of milk residue. 5 ppm and 10 ppm non-fat dry milk Tests per kit: Up to 20 Reveal for Total Milk Allergen is intended for the qualitative analysis of milk residue (casein or whey) in liquid products (e.g., juices and sorbet), clean-inplace rinses, and on environmental surfaces. Sensitivity: Screens at 5 ppm total milk 5 minutes Storage: Room temperature, C Tests per kit: 24 Positive (-) sample (+) sample Negative 8471 Alert for Total Milk Allergen 8432S Allergen Environmental Swabbing Kit Reveal for Total Milk Allergen 8429 Allergen Extraction Kit 8432S Allergen Environmental Swabbing Kit 9590 Reveal AccuScan III Reader 52 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

7 For Total Milk Allergen Veratox for Total Milk Allergen is used for the quantitative analysis of milk residue in food products such as juices, wine, sauces or sorbets. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm non-fat dry milk s 8470 Veratox for Total Milk Allergen Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

8 For Mustard Allergen Veratox for Mustard Allergen is used for the quantitative analysis of mustard proteins in food products such as spices, sauces, dressings, meats and cleanin-place rinses. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm mustard s 8400 Veratox for Mustard Allergen 8432S Allergen Environmental Swabbing Kit Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

9 Alert For Peanut Allergen Reveal For Peanut Allergen Alert for Peanut Allergen is used as a screening tool to test for peanut residue in food products such as cookies, crackers, chocolate bars, ice cream and cereals, and on environmental surfaces. Control provided: 5 ppm peanut Tests per kit: Up to 20 Reveal for Peanut Allergen is intended for the qualitative analysis of peanut residue in food products (e.g., cookies, crackers, chocolate bars, ice cream and cereals), clean-in-place rinses, and on environmental surfaces. Sensitivity: Screens at 5 ppm peanut 10 minutes Storage: Room temperature, C Tests per kit: 25 Positive (-) sample (+) sample Negative 8431 Alert for Peanut Allergen 8432S Allergen Environmental Swabbing Kit Reveal for Peanut Allergen 8429 Allergen Extraction Kit 8432S Allergen Environmental Swabbing Kit 9590 Reveal AccuScan III Reader Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

10 For Peanut Allergen Veratox for Peanut Allergen is used for the quantitative analysis of peanut residues in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm peanut Approvals: AOAC-RI # s 8430 Veratox for Peanut Allergen 56 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

11 Alert For Soy Allergen For Soy Allergen Alert for Soy Allergen is used as a screening tool to test for soy protein in products such as cookies, crackers, granola/power bars, ice cream, salad dressing, cereal, juice, milk, clean-in-place rinses, and on environmental surfaces. 10 ppm soy Tests per kit: Up to 20 Veratox for Soy Allergen is used for the quantitative analysis of soy protein in food products such as cookies, crackers, granola/power bars, ice cream, salad dressings, cereal, liquid products (e.g., juices and milk) and clean-in-place rinses. Range of quantitation: ppm 0, 10, 25, 50 and 100 ppm soy (-) sample (+) sample s 8411 Alert for Soy Allergen 8432S Allergen Environmental Swabbing Kit Prod#. Product description 8410 Veratox for Soy Allergen Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

12 Alert For Soy Flour Allergen For Soy Flour Allergen Alert for Soy Flour Allergen is used as a screening tool to test for soy flour protein in food products such as cookies, crackers, chocolate bars and cereals, and on environmental surfaces. 5 ppm and 10 ppm soy flour Tests per kit: Up to 20 Veratox for Soy Flour Allergen is used for the quantitative analysis of soy flour protein in food products such as cookies, crackers, chocolate bars and cereals. Range of quantitation: 2.5 ppm 25 ppm 0, 2.5, 5, 10 and 25 ppm soy flour (-) sample (+) sample s 8491 Alert for Soy Flour Allergen 8432S Allergen Environmental Swabbing Kit Veratox for Soy Flour Allergen 58 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

13 BioKits for Food Allergens Reveal 3-D BioKits for Food Allergens BioKit Assays Reveal 3-D Technology The unique hand-held device with 3-line readout: a line confirms the method has been performed successfully and two further lines differentiate low and high levels of detection. Simple Tools for Food Allergen Control The Reveal 3-D Tests make it simple to screen for the presence of low levels of allergens in food products and environmental swabs virtually anywhere. Sample preparation and testing take less than 10 minutes. Each kit contains everything needed (including swabs) to perform 10 single use tests (100 test kits also available). The BioKits range of ELISA test kits have been developed for use in the detection of quantification of allergens in foods and environmental swabs. BioKits tests are being employed by food manufacturers and regulatory laboratories to ensure accurate labelling and to accidental crosscontamination by these allergens. All tests have undergone a rigorous validation process, investigating the sensitivity, specificity, cross-reactivity and robustness of the assay. BioKits Assay Quantification (ppm) Units Wells BLG BLG 48 Casein Casein 96 Egg Egg White Protein 48 Gluten 3 50 Gluten 96 Peanut 1 20 Peanut 96 Sesame Sesame 48 Shellfish 1 20 Shellfish Protein 48 Walnut Walnut G Reveal 3-D Almond M Reveal 3-D Casein Q Reveal 3-D Egg P Reveal 3-D Gluten E Reveal 3-D Hazelnut L Reveal 3-D Peanut S Reveal 3-D Shellfish K Reveal 3-D Soya 10 Prod#. Product description Y BLG Assay Kit W Casein Assay Kit T Egg Assay Kit Y Gluten Assay Kit Q Peanut Assay Kit X Sesame Assay Kit K Shellfish Assay Kit J Walnut Assay Kit J BioKits Allergen Swabbing Kit contains 100 swabs and buffer Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/

14 Food Allergen and Adulteration Test Kits BioKits for Food Allergens DNA Allergen Selection Module BioKits for Food Adulteration Adulteration Testing Principle of the Kit The BioKits DNA Extraction (GMO & Allergen) kit enables sample extraction using proprietary magnetic bead technology. It is used in combination with the BioKits Allergen Selection Module which involves amplification by PCR and detection by agarose gel electrophoresis. The Module contains a choice of allergen DNA specific PCR mastermixes, packaged as color-coded Pods. These are all-in-one mixes, requiring only the addition of template and Taq polymerase. The mastermixes are pink in color because they contain gel loading dye which eliminates the need for a procedural step later on. Spike and DNAs are also included to ensure the reliability of the tests. The agarose gel detection method has been simplified and the combination of high quality extraction and PCR reagents aids the interpretation of results for the detection of peanut, soya and cow s milk. Suitable applications: Samples tested: Limit of detection: Hands-on time: Products required: Shelf-life: Storage: Qualitative identification of allergen DNA in food Up to 100 sample extractions per extraction kit Up to 300 PCR reactions per module Soya content 5 ppm; cow s milk and caseinate 5 ppm; peanut 10 ppm < 5 hours (for 10 ) for extraction and PCR BioKits DNA extraction kit (GMO & Allergen) and BioKits Allergen Selection Module Up to 12 months from manufacture As specified in product insert (requirement for storage at ambient temperatures, at 2 8 C and below -18 C) Pasta Quality Control (PQC) Assay Kit A sensitive and specific semi-quantitative test for the detection of common wheat adulteration in a range of pasta and noodle products. Soya Assay Kit A member of the legume family, soya protein is commonly used as a texturizer, emulsifier and protein filler in the food processing industry. It is also a known allergen. The BioKits Soya Assay kit is a quantitative ELISA (0.5% detection limit) which specifically detects soya protein in raw and cooked/canned foods, meats/meat products. BioKits Assay Quantification (ppm) Units Wells PQC 3 15% Common Wheat 96 Soya % Soya Protein C BioKits PCR MASTERMIX POD (Peanut) N BioKits PCR MASTERMIX POD (Soya Lecithin) H BioKits PCR MASTERMIX POD (Beef) N BioKits DNA EXTRACTION KIT (GMO & Allergen) Y BioKits MAGNETIC RACK M Pasta Quality Control Assay Kit T Soya Assay Kit 60 Sales & Service: 800/ (USA/Canada) or 517/ Fax: 517/