AgraQuant F.A.S.T. Egg. Test Kits available: AgraQuant. AgraQuant F.A.S.T. Cashew. AgraQuant F.A.S.T. Peanut

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1 AgraQuant Allergens ELISA Tests AgraQuant Allergen Test Kits available: NEW The fastest ELISA method on the market! Save time! Kit AgraQuant Almond AgraQuant Casein AgraQuant Cashew AgraQuant Egg AgraQuant Hazelnut AgraQuant Macadamia nut AgraQuant Mustard AgraQuant Peanut Order number COKAL0748F COKAL1248F COKAL3148F COKAL1848F COKAL0348F COKAL1648F COKAL2148F COKAL0148F The AgraQuant Allergen Test kits are sandwich enzyme immunoassays (ELISA) for the fast detection and quantitative determination of allergens in foods, environmental samples and rinse waters. Your benefits: Save time fastest allergen ELISA on the market (with an extraction time of 1 min and assay performance of min) Protect your business with accurate results Optimize your workflow and reduce individual errors due to unique extraction capsules technology Increase lab productivity by using the same extract for different allergens Better ordering planning: 12 months shelf life AgraQuant Allergen Test Kits available: Kit AgraQuant Almond AgraQuant Casein AgraQuant Cashew AgraQuant Crustacea AgraQuant Egg white AgraQuant Fish AgraQuant Gluten AgraQuant Gluten G12 AgraQuant Hazelnut AgraQuant Lupin AgraQuant Lysozym AgraQuant Milk AgraQuant Mustard AgraQuant Ovalbumin AgraQuant Peanut AgraQuant Pistachio AgraQuant Sesame AgraQuant Soy AgraQuant Walnut AgraQuant β-lactoglobulin Order number COKAL0748 COKAL COKAL3148 COKAL2248 COKAL0848 COKAL248 COKAL0248 COKAL00 COKAL0348 COKAL148 COKAL2848 COKAL2448 COKAL2148 COKAL2948 COKAL0148 COKAL2748 COKAL1948 COKAL0448 COKAL0948 COKAL48 Kit AgraQuant Allergen Swabbing kit ( swabs) Order number COOLS01

2 Sampling Consideration must be taken that the food may contain an uneven distribution of allergens (spot contamination). It is important to test a representative portion of food as only a small amount of material is tested with the AgraQuant Allergen test. So far there are no official regulations on sampling for food allergen testing. One reason for this is the different nature of samples for food allergens as they can vary from solid, dry powders to viscous and liquid form. A different approach is necessary for each type of sample matrix. Swabbing The AgraQuant Allergen Swabbing Kit is an accessory kit used to collect environmental swab samples from surfaces on which allergenic proteins may be present. The extraction method and its efficiency - together with the high affinity of the antibody used in the assay determines the performance of the assay and thus the limit of detection. Assay Principles The test is based on the high affinity antigen-antibody reaction and allows the detection of allergens in food, rinse waters and swab samples (environmental samples). Extracted samples or standards are transferred to wells in the microtiter plate that are coated with allergen specific antibodies. Allergen proteins in the extract or standard will bind to the pre-coated antibodies and build an antigen-antibody complex. In a washing step non-bound quantities are removed, so that allergen-specific-enzymecoupled antibodies (conjugate) can bind to captured allergens in the next step. Excess conjugate is removed once more by washing. The addition of a colorless substrate starts off the color reaction. The final addition of stop solution causes another drift in color that is then measured at nm and that is proportional to the samples allergen content.

3 The est Allergen ELISA Method on the market The new AgraQuant line combines the fastest extraction method with a rapid assay, resulting in the fastest time-to-result within only 31 minutes. New extraction technology With the new capsules technology you cut down extraction to a minimum of only 1 minute. Therefore you just simply add both extraction capsules to the tube containing the sample and top it with hot (80-0 C) distilled water and shake it until sample and capsules are dissolved. Furthermore you can use the same extract for testing different allergens of the AgraQuant line. 1min extraction procedure: 4 ml 4 ml 4 ml 4 ml Weigh in 1 g sample and add together with extraction additive 1 & 2 to an empty centrifuge tube 2. Bring distilled water to 80-0 C and add ml hot, but not boiling water to centrifuge tube 4 ml 4 ml 4 ml 4 ml 4 ml or Shake tube vigorously for 1 seconds until sample and capsules are dissolved 4. Transfer an aliquote of the extract to a 1. or 2 ml reaction vial and centrifuge at 8000g for min or alternatively filtrate through a pleated filter and use filtrate in the test

4 Improved Assay performance Another advantage of the new AgraQuant line is the improved assay time of only minutes. 1 Dispense μl sample extract or standards into transfer wells (blue) 2 Transfer 0 μl sample extract or standards into the antibody coated wells (colourless) using a multichannel pipette and incubate for min 3 Wash times with wash buffer 4 Tap dry washed wells Pipette 0 μl conjugate solution into the antibody coated wells and incubate for min, wash the wells afterwards as described in step 3 & 4 6 Pipette 0 μl substrate solution into the wells and incubate for min in the dark 7 Add 0 μl stop solution into the antibody coated wells 8 Read Results at nm with an ELISA reader Advantages performing AgraQuant Allergen tests Save time Fastest allergen ELISA method on the market (with an extraction time of 1min and assay performance of min) Protect your business avoid costly recalls with accurate and reliable results Optimize your workflow Reduce individual errors by using our unique extraction capsules technology Increase lab productivity Same extract for different allergens Improve order planning 12 month shelf life All products of the AgraQuant Allergen kit range were developed in cooperation with ifp (

5 Validation data AgraQuant Almond Casein Egg Cashew Mustard Hazelnut Macadamia nut Peanut Limit of detection Foodstuff Swab Sponge Rinse water 0. ppm 0.0 μg/swab 0. μg/sponge 0 μg//l 0.2 ppm 0.02 μg/swab 0.2 μg/sponge μg//l 0. ppm 0.0 μg/swab 0. μg/sponge 0 μg//l 1 ppm 0.1 ug/swab 1 ug/sponge 0ug/L 0. ppm 0.0 ug/swab 0. ug/sponge 0 ug/l 1 ppm 0.1 μg/swab 1 μg/sponge 0 μg//l 1 ppm 0.1 μg/swab 1 μg/sponge 0 μg//l 0. ppm 0.0 μg/swab 0. μg/sponge 0 μg//l Quantification range 1 2 ppm 1 2 ppm 1 2 ppm 1 2 ppm 1 2 ppm 2 2 ppm 1 2 ppm 1 2 ppm Units Whole almond Casein Whole egg powder total cashew total mustard Whole hazelnut Whole macadamia Whole peanut Specificity with samples tested with samples tested with samples tested Cross-reactivity to Pistachio Cross-reactivity to rape seed Cross-reactivity to pecan nut and Walnut Slight cross-reactivity to grey poppy seed No crossreactivity with samples tested Limit of detection in spiked commodities 2 ppm 2 ppm 2 ppm 2 ppm 2 ppm 2 ppm 2 ppm 2 ppm Romer Labs ProductFocus AgraStrip Allergens

6 Validation data AgraQuant Almond β-lactoglobulin Casein Cashew Crustacea Egg white Fish Gluten G12 Gluten Hazelnut Limit of Detection 0.2 ppm 1. ppb 0.04 ppm 0.2 ppm 0.9 ppb 0.0 ppm 1.4 ppm 2 ppm 0.6 ppm 0.3 ppm Quantification range 0.4 ppm 0 ppb ppm 2 60 ppm 0 ppb 0.4 ppm 4 0 ppm 4 0 ppm 4 1 ppm 1 ppm Units Almond β-lactoglobulin Casein Cashew Tropomyosin Egg white protein Cod Gluten Gluten Hazelnut Specificity in tested matrices in tested matrices Slight crossreactivity to Ewe s milk and Goat s milk Slight cross reactivity to Pistachio in tested matrices No crossreactivity in tested matrices in tested matrices Detects Wheat, Rye, Triticale and rye Detects Wheat, Rye, Triticale and rye No crossreactivity in tested matrices Limit of detection in spiked commodities 0.63 ppm ppb 0.37 ppm 3.12 ppm 2 ppb 0.63 ppm 6.2 ppm ppm 3.7 ppm 2. ppm procedure 1 g in ml Buffer at 60 C 0. g in ml Buffer at 60 C 1 g in ml Buffer at 60 C 1 g in ml Buffer at C 1 g in ml Buffer at 60 C 0.2 g in 2. ml solution (0 C, min) + 7. ml 80 % EtOH 1 g in ml % EtOH 1 g in ml Buffer at 60 C time 2 min 1 min 1 min 2 min Sample dilution no 1: 1:0 no

7 Validation data AgraQuant Lupin Lysozyme Milk Mustard Ovalbumin Peanut Pistachio Sesame Soy Walnut Limit of Detection 0.2 ppm 2 ppb 0.0 ppm 1 ppm 4 ppb 0.1 ppm 0.13 ppm 0.2 ppm 16 ppb 0.3 ppm Quantification range 2 ppm 2 - ppb 0.4 ppm 2 60 ppm 2 00 ppb 1 ppm 1 ppm 2 ppm 00 ppb 2 60 ppm Units Lupin Lysozyme Milk proteins Mustard Ovalbumin Peanut Pistachio Sesame Soy-Trypsin- Inhibitor Walnut Specificity Slight crossreactivity to Chickpea, Soy, Lentil and beef Cross-reactivity to radish, cabbage, rape, brown mustard, black mustard in tested matrices in tested matrices Slight cross reactivity to oats and bean Slight crossreactivity to Sesame Slight crossreactivity to Pecan nut Limit of detection spiked commodities 1.9 ppm 1.62 ppb 0. ppm 3.7 ppm 2 ppb 2. ppm 2. ppm 3.7 ppm 63 ppb 3.7 ppm procedure 1 g in ml Buffer at 60 C 0. g in ml Buffer at 60 C 1 g in ml Buffer at 60 C time 2 min Sample dilution no no - only 0 % meat and sausage sample 1: no Romer Labs ProductFocus AgraStrip Allergens

8 Related Romer Labs Food Allergen Products AgraStrip Lateral flow devices Kit LOD Item No. AgraStrip Almond 2 ppm Almond COKAL07AS AgraStrip β-lactoglobulin 0. ppm BLG COKALAS AgraStrip Brazil nut ppm Brazil nut COKAL17AS AgraStrip Casein 2 ppm Casein COKAL12AS AgraStrip Cashew/Pistachio ppm Cashew/Pistachio COKAL13AS AgraStrip Coconut ppm Coconut COKAL26AS AgraStrip Crustacea ppm Crustacea (wet) COKAL22AS AgraStrip Egg 2 ppm dried whole egg COKAL18AS AgraStrip Gluten G12 ppm Gluten COKAL00AS AgraStrip Gluten 4 ppm Gluten COKAL02AS AgraStrip Hazelnut ppm Hazelnut COKAL03AS AgraStrip Lupin ppm Lupin COKAL1AS AgraStrip Macadamia nut 2 ppm Macadamia nut COKAL16AS AgraStrip Milk 1 ppm milk protein COKAL24AS AgraStrip Mustard 2 ppm Mustard COKAL21AS AgraStrip Peanut 1 ppm Peanut COKAL01AS AgraStrip Sesame ppm Sesmae COKAL19AS AgraStrip Soy 2 ppm Soy COKAL04AS AgraStrip Walnut ppm Walnut COKAL09AS EnzymeFast Enzymatic food analysis Kit LOD Item No. EnzymeFast Lactose/D-Galactose EnzymeFast Lactose/D-Glucose For g weighed sample: 0.004g/0g lactose 0.002g/0g D-galactose For g weighed sample: 0.004g/0g lactose 0.002g/0g D-glucose COKEF00 COKEF0700 Imprint published by Romer Labs Division Holding GmbH, Technopark 1, 34 Tulln, Austria, Tel: , Fax: , marketing@romerlabs.com Editors: Lukas Frank IMPRINT

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