Report of the ISTA Proficiency Test Committee
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1 Report of the ISTA Proficiency Test Committee Günter Müller Year of the Review 2013
2 Proficiency Test Committee Members Chair - Günter Müller Vice - Didier Demilly Members - Laura Carlson - Sharon Davidson - Sinikka Köylijärvi - Gillian McLaren - Mari Jürmann - Zita Ripka - Håkon Tangerås - Benno Voit - Ruojing Wang - Gisela Wustmann - Rita Zecchinelli
3 Content Assessment of the regular Proficiency Test Rounds 2013 on Phalaris canariensis, Pisum sativum, Brassica napus and Pinus sylvestris seed - Test leader s introduction - In-round ratings of accredited and voluntary participants - Troubleshooting for poor results - Presentation of the different methods for germination testing used by participants Results of the third Seed Identification Training Test Round Scheduled Proficiency Test Rounds
4 PT 13-1 Phalaris canariensis Test Leader: Ruojing Wang Scope: P, OSD, G Samples prepared: 3 x 160 Time spent: 236 hours Money spent: 210 $ Date - Presenter Name
5 PT 13-1 Phalaris canariensis Scope Purity Analysis Accredited Labs N=81 2% 2% Voluntary Labs N=42 5% 2% 7% 96% 86% A B C BMP Date - Presenter Name
6 PT 13-1 Phalaris canariensis Scope OSD Accredited Labs N=81 Voluntary Labs N=42 5% 2% 26% 33% 31% 62% 17% 24% A B C BMP Date - Presenter Name
7 PT 13-1 Phalaris canariensis Lot 1 Seeds retrieved and identified Species Seeds added Retrieval Rate (%) Hordeum jubatum Sporobolus cryptandrus Eragrostis curvula Panicum maximum
8 PT 13-1 Phalaris canariensis Lot 2 Seeds retrieved and identified Species Rapistrum rugosum Seeds added Retrieval Rate (%) Holcus lanatus Phalaris arundinacea Eragrostis tef
9 PT 13-1 Phalaris canariensis Lot 3 Seeds retrieved and identified Species Alopecurus aequalis Seeds added Retrieval Rate (%) Galium aparine Helianthus annuus Lapsana communis
10 PT 13-1 Phalaris canariensis Scope Germination Testing Accredited Labs Voluntary Labs 1% N=80 N=43 15% 4% 2% 14% 7% 80% 77% A B C BMP Date - Presenter Name
11 PT 13-1 Phalaris canariensis Germination Test Results Lot % Normal Seedlings Standard Deviation % Abnormal Seedlings
12 PT 13-1 Phalaris canariensis Normal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
13 PT 13-1 Phalaris canariensis Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP PP TP O PT Mean Values
14 PT 13-1 Phalaris canariensis Applied Germination Test Methods (Temperature) Temp. C Users (accr. Labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 15<=> <=> PT Mean Values
15 PT 13-1 Phalaris canariensis Applied Germination Test Methods (Pre-treatment) Pre-treatment Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total KNO Light Not Treated Pre-chill PT Mean Values
16 PT 13-2 Pisum sativum Test Leader: Sylvia Zanetti Scope: G Samples prepared 3 x 196 Time spent: 48 hours Money spent:? (no info) Date - Presenter Name
17 PT 13-2 Pisum sativum Scope Germination Testing Accredited Labs N=89 Voluntary Labs N=66 16% 4% 20% 8% 43% 80% 29% A B C BMP Date - Presenter Name
18 PT 13-2 Pisum sativum Germination Test Results Lot % Normal Seedlings Standard Deviation % Abnormal Seedlings
19 PT 13-2 Pisum sativum Normal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
20 PT 13-2 Pisum sativum Abnormal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
21 PT 13-2 Pisum sativum Non-germinated Seeds Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
22 PT 13-2 Pisum sativum Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP PP TP Sand Soil PT Mean Values
23 PT 13-1 Pisum sativum Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total PT Mean Values
24 PT 13-3 Brassica napus Test Leader: Günter Müller Scope: P, OSD, G, TZ, Moi Samples prepared: 6 x 210 Time spent: 259 hours Money spent: 48 Euro Date - Presenter Name
25 PT 13-3 Brassica napus Scope Purity analysis Accredited Labs N=114 1% 3% Voluntary Labs 9% N=43 14% 77% 96% A B C BMP Date - Presenter Name
26 PT 13-3 Brassica napus Scope OSD 16% Accredited Labs N=114 2% 4% 23% Voluntary Labs N=43 45% 9% 78% 23% A B C BMP Date - Presenter Name
27 PT 13-1 Brassica napus Lot 1 Seeds retrieved and identified Species Seeds added Retrieval Rate (%) Galium aparine Sinapis arvensis Stellaria media Thlaspi arvense
28 PT 13-1 Brassica napus Lot 2 Seeds retrieved and identified Species Chenopodium album Seeds added Retrieval Rate (%) Lactuca sativa Phacelia tanacetifolia Vaccaria hispanica
29 PT 13-1 Brassica napus Lot 3 Seeds retrieved and identified Species Fallopia convolvulus Seeds added Retrieval Rate (%) Raphanus sativus Rumex crispus Sinapis alba
30 PT 13-3 Brassica napus Scope Germination Testing Accredited Labs 5% 4% N=112 Voluntary Labs 17% N=46 13% 7% 56% 20% 78% A B C BMP Date - Presenter Name
31 PT 13-3 Brassica napus Germination Test Results Lot % Normal Seedlings Standard Deviation % Abnormal Seedlings
32 PT 13-3 Brassica napus Normal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
33 PT 13-3 Brassica napus Abnormal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
34 PT 13-3 Brassica napus Non-germinated Seeds Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
35 PT 13-3 Brassica napus Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP PP TP Sand O PT Mean Values
36 PT 13-3 Brassica napus Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total <=> PT Mean Values
37 PT 13-3 Brassica napus Applied Germination Test Methods Pre-treatment Pretreatment Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total KNO Light Not Treated Pre-chill PT Mean Values
38 PT 13-3 Brassica napus Scope Viability Testing Accredited Labs N=49 Voluntary Labs N=56 4% 4% 7% 7% 16% 16% 76% 70% A B C BMP Date - Presenter Name
39 PT 13-3 Brassica napus Viable Seeds Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
40 PT 13-3 Brassica napus Scope Moisture Content Determination Accredited Labs N=100 Voluntary Labs N=47 17% 3% 6% 26% 4% 47% 74% 23% A B C BMP Date - Presenter Name
41 PT 13-3 Brassica napus Moisture Content Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
42 PT 13-3 Pinus sylvestris Test Leader: Sergio Pasquini Scope: G Samples prepared: 3 x 100 Time spent: 65 hours Money spent: 714 Date - Presenter Name
43 PT13-3 Pinus sylvestris Scope Germination Testing Accredited Labs N=24 Voluntary Labs N=25 8% 21% 28% 64% 8% 13% 58% A B C BMP Date - Presenter Name
44 PT13-3 Pinus sylvestris Germination Test Results Lot % Normal Seedlings Standard Deviation % Abnormal Seedlings
45 PT13-3 Pinus sylvestris Normal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
46 PT13-3 Pinus sylvestris Abnormal Seedlings Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
47 PT13-3 Pinus sylvestris Non-germinated Seeds Distribution Frequency ,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
48 PT13-3 Pinus sylvestris Applied Germination Test Methods (Substrate) Substrate Not reported Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total PP TP PT Mean Values
49 PT13-3 Pinus sylvestris Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total <=> PT Mean Values
50 PT13-3 Pinus sylvestris Applied Germination Test Methods (Pre-treatment) Pretreatment Not Treated Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total Pre-chill PT Mean Values
51 Seed Identification Training Test Round Test Leader: Günter Müller Scope: OSD Samples prepared: 150 Time spent: 55 hours Money spent: 25 Date - Presenter Name
52 Seed Identification Training Test Round 17% Voluntary Labs 3% N=119 3% 77% A B C BMP Date - Presenter Name
53 Seed Identification Training Test Round (Part 1) Species Seeds added Retrieval Rate (%) Pascopyrum smithii Crotalaria pallida Luffa acutangula Carica papaya Momordica charantia Holcus lanatus
54 Seed Identification Training Test Round (Part 2) Species Trifolium resupinatum Seeds added Retrieval Rate (%) Sinapis alba Plantago major Festuca rubra Bromus sterilis Cynoglossum officinale
55 Seed Identification Training Test Round (Part 3) Species Seeds added Retrieval Rate (%) Bromus sterilis Geranium dissectum Aethusa cynapium Trifolium repens Lapsana communis Agrosthemma githago
56 Seed Identification Training Test Round (Part 4) Species Seeds added Retrieval Rate (%) Rumex acetosella Convolvulus arvensis Bromus hordeaceus Alopecurus myosuroides Glycine max
57 Scheduled Proficiency Test Rounds Round # Species 14-3 Poa pratensis Scope P, OSD, G Seed Identification Training Test Round 15-1 Glycine max G, M 15-1 Fagus sylvatica P, G, TZ, M, OIC 15-2 Trifolium pratense P, OSD, G, TZ, OIC
58 Thank you very much for your attention! Date - Presenter Name
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