Estimates of Genetic Parameters and Correlation in Catkin and Seed Traits of Salix alba

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1 International Journal of Current Microbiology and Applied Sciences ISSN: Volume 6 Number 5 (2017) pp Journal homepage: Original Research Article Estimates of enetic arameters and Correlation in Catkin and Seed Traits of Salix alba.a. aray, S. A. angoo, S.J.A. Bhat*, Amir Bhat and A. A. array Faculty of Forestry, Benhama, anderbal, J&K , India *Corresponding author A B S T R A C T K e y w o r d s Catkin-seed, Salix alba, CT s, Correlation and heritability. Article Info Accepted: 17 April 2017 Available Online: 10 May 2017 Twenty candidate plus trees (CT s) of Salix alba were selected from different locations of Kashmir valley following comparison tree method. Significant variation and association were observed among all the selected CT s for catkin and seed traits. CT-12 had maximum for majority of the characters. Heritability in broad sense was high for all the characters under study with maximum (0.936) for capsule diameter followed by high to moderate gain indicating the additive gene action. ermination percentage was found to have positive and significant correlation with catkin and seed weight. CT-12, CT-6 and CT-53 were found to be best on the basis of these characters. Introduction The Salix species belong to the family Salicaceae. There are about species of Salix known from all around the world which are distributed mostly in the Northern Hemisphere (Argus, 1997; Skvortsov, 1999). In India there are about 33 Salixspecies which have majorly been categorized as shrubs except Salix alba, S. babylonica, S. daphnoids, S. fragilis, S. elegasand S. tetrasperma. In Kashmir valley, the genus Salix is represented by 23 species of which 15 reach alpine/subalpine limits (Dhar and Kachroo, 1983). The genus Salixis very heterogeneous and shows considerable variation in size, growth form, and crown architecture. The Salix species are ecofriendly, multipurpose, fast growing and are widely used for plantation world over. These are being cultivated for a variety of end uses viz. baskets, cricket bats, hurdles, furniture, plywood, paper and pulp, rope making etc (Verwijst, 2001; Kuzovkina et al., 2008). Salix alba is a moderate to a large deciduous tree with ascending branches and spreading but light crown, attaining a height of m and a diameter of 60 cm. under its native habitat, trees are known to attain a height of 30 m and I m in diameter. Bark is reddish green or brown, smooth in younger trees, fissuring longitudinally with age. Leaves are long lanceolate, acuminate silky, grayish green, silvery white underneath, finely serrated, petiole about 1 cm; shoots are first silky then olive green and very supple. 1601

2 Flowers male and female in separate catkins. Fruits ovoid-conic capsules. Seeds minute, yellow, ovoid with silky hairs which help in dispersal (Luna, 1995). The growth statistics of Salix alba can be compared with a good clone of poplar. In order to diversify the plantation of tree species with integration of agriculture crops, willow is most eco-friendly and farmer s choice. The cricket bat and artificial limbs industry is solely depending on the wood of Salix alba and there is ready made market for willow based wood industry (Saini and Sharma, 2001). White willow is primarily utilized for cricket bats and polo balls, fruit boxes, artificial limbs, matchwood, honey-comb frames, tool handles, fibre-boards, agricultural implements, boats etc. used as a fence post, it is as durable as oak (Luna, 1995). The effectiveness of tree improvement programme depends upon the nature and magnitude of existing genetic variability and also on the degree of transmission of traits or heritability (Zobel and Talbert, 1984), because genetic variation is the fundamental requirement for maintenance and long-term stability of forest ecosystem. The rate of tree improvement can be increased or decreased by influencing the selection differential or heritability, or by reducing the total variance (Bagchi, 1995). The knowledge of genetic variability and association between catkin and seed traits is considered to provide considerable help in genetic improvement of the species. Hence, the present investigation was envisaged to evaluate the source variation in different catkin and seed traits of Salix alba. Material and Methods Twenty CT s of Salix alba were selected following comparison tree method. Mature catkins (yellowish) were collected from these CT s in the month of May 2015 when the capsules of catkins were ready to burst (mature). Care was taken to collect dry catkins from the marked trees. Sufficient catkins were 1602 collected from each CT. These catkins were analyzed for various characteristics viz. catkin length (cm), catkin width, number of capsules/catkin, capsule diameter, 100- catkin weight (g), 1000-seed weight (mg) and germination percentage. The germination percentage was worked out as per the following formula: Analysis of variance was carried out following the procedure given by omez and omez (1984). henotypic coefficient of variation (CV) and enotypic coefficient of variation (CV) were computed as per the method suggested by Burton (1952): and Where, Vp is phenotypic variance and Vg is genotypic variance Heritability (H 2 ) in broad sense was estimated as per the procedure presented by Burton and Dewane (1953), Johnson et al., (1955), Hanson et al., (1956) and Allard (1960). H 2 = Where, Vg is genotypic variance and Vp is phenotypic variance enetic advance (A) was worked out using the formula given by Lush (1949) and Johnson et al., (1955). A = 0.5 x K Where, Vg is genotypic variance of the trait, Vp is phenotypic variance of the trait and K is selection differential, (K=2.06 at 5 percent selection intensity).

3 enetic gain () was estimated as per the method suggested by Johnson et al., (1955). = advance of the trait Where, A is genetic enotypic (r g ) and phenotypic (r p ) correlation coefficients were worked out for all the possible pairs of characters (oulden, 1952). r g and r p Where, Cov.xy(g) and Cov.xy(p)are the genotypic and phenotypic covariance for a pair of characters and Vx(g),Vy(g)Vx(p)and Vy(p) are the genotypic and phenotypic variances for the respective characters. Results and Discussion In the present investigation, significant variation was observed among the CT s of Salix alba with respect to catkin and seed traits (Table 1). These traits varied from 4.6 cm to 9.9 cm for catkin length, 4.4 mm to 10.2 mm for catkin width, 53.0 to for number of capsules per catkin, 1.3 mm to 4.0 mm for capsule diameter, 56.0g to g for 100-catkin weight, 61.2 mg to mg for 1000-seed weight and 79.6 % to 97.6 % for germination. Analogous with the present findings, significant variation was reported in seed and pod traits of candidate plus trees of ongamia pinnata (Kaushik et al., 2007; Sahoo et al., 2011; avithra et al., 2013 and awali et al., 2015). Tomar and Rattan (2012) revealed significant variation in fruit and seed characters of Hippophae salicifolia. Wani and Ahmad (2013) explored variability in germination parameters of candidate plus trees of Madhuca indica. Significant variation in Albizia procera for pod, seed and germination characters was observed by era et al., (2001). Studies conducted with respect to variation in pod, seed and germination parameters in Jatropa curcas (inwal et al., 2005; Rao et al., 2008), Albizia lebbek (Luna et al., 2006) and Dalbergia sissoo (Srivastava et al., 2001, Singh and Sofi, 2011) are in congruity with the present findings. enetic parameters of catkin and seed characters of Salix alba have been presented under table 2. It is clearly evident that phenotypic variance and phenotypic coefficient of variation were greater than the respective genotypic variance and genotypic coefficient of variation for all the characters under study. Fair differences between CV and CV of the characters under study indicate the heritable nature of these traits. All catkin and seed characters exhibited high heritability ranging from for capsule diameter to for seed weight. High heritability estimates for all pod and seed traits were reported by Divakara and Das (2011) and awali et al., (2015) in ongamia pinnata which is in conformity with the present findings. inwal et al., (2005) reported high heritability estimates for germination in Jatropa curcas. Johnson et al., (1955) reported that heritability estimates along with expected gain is more useful and realistic than heritability alone predicting the resultant effect for selecting the best genotype. In this study genetic advance and genetic gain showed sizeable range indicating the scope for improvement in these characters. enetic gain was high to moderate for all the characters. High heritability along with high to moderate genetic gain for catkin and seed traits indicate the influence of additive gene action in the expression of these characters Association is usually determined by studying the correlations existing between various characters under study. The phenotypic correlation coefficient gives the extent of relationship between the two characters which include the genetic and environmental 1603

4 influences whereas genotypic correlation coefficient indicates the real association between two traits and may be useful in selection (Johnson et al., 1955). CT s Table.1 Variability in catkin and seed traits of selected CT s of Salix alba Catkin length (cm) Catkin width Capsules/ Catkin Capsule diameter Capsule Weight (g) Seed Weight (mg) erminatio n percentage CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT CT Mean CD SE (m) CV % Min Max

5 Table.2 Estimates of genetic parameters of catkin and seed characteristics of Salix alba Coefficient of Variance S.No. arameters Variation enetic components Vp Vg CV CV H 2 A 1 Catkin length (cm) Catkin width (cm) Capsules/catkin Capsule diameter Catkin weight (g) Seed weight (mg) ermination percentage Table.3 enotypic () and phenotypic () correlation matrix between catkin and seed characteristics of Salix alba arameters Type Catkin length (cm) Catkin width Capsules/catkin Capsule diameter 100-Catkin weight (g) 1000-Seed weight (mg) **=significant at 5%, *=significant at 1% Catkin width 0.212* 0.235* Capsules/ catkin 0.577** 0.581** Capsule diameter 0.263* 0.267* Catkin weight (g) 0.385** 0.392** 0.202* 0.209* * 0.286* 1000-Seed weight (mg) 0.287* 0.295* ** 0.313** 0.417** 0.425** ermination percentage ** 0.482** 0.359** 0.366** 1605

6 Correlation coefficients of catkin, seed characteristics have been worked out between all the possible pairs and presented under table 3. Catkin length was positively correlated with almost all other catkin and seed characters having maximum correlation (0.577, 0.581) with number of capsules per catkin. Catkin weight displayed positive correlation with seed weight (0.417, 0.425). ermination percentage was positively correlated with catkin weight (0.476, 0.482) and seed weight (0.359, 0.366). Tomar and Rattan (2012) have reported highly significant correlation between fruit length and fruit width in Hippophae salicifolia. ositive and significant correlation coefficients were reported between cone and seed traits in inus gerardiana (Singh and Chaudhary, 1993) which corroborates the present results. There was positive and significant correlation (genotypic and phenotypic) between 100- catkin weight and 1000-seed weight. In consonance with the present findings, Sahoo et al., (2011) while working on CT s of ongamia pinnata has reported high estimates of genotypic correlations than the corresponding phenotypic correlations between pod length and pod breadth; 100-pod weight, and pod thickness; 100-seed weight and 100-pod weight indicating the presence of strong inherent association. ermination percentage was found to have positive and significant correlation with 100-catkin weight and 1000-seed weight. ositively significant correlation between germination and seed weight has been reported by Singh and Sofi (2011) in Dalbergia sissoo and avithra et al., (2013) in ongamia pinnata. In conclusion, from the present investigation, it is clear that there existed wide variation among the selected CT s of Salix alba with respect to catkin and seed characters. CT-12, CT-6 and CT-53 were found to be best on the basis of these characters which revealed promise in their further exploitation for plantation and selection for improvement. High genotypic correlation coefficient between pod and seed characters revealed that the traits are genetically controlled and selection can be very effective in tree improvement programmes of this tree species. References Allard, R.W rinciples of plant breeding. John Wiley & Sons. Inc. New York, p 485. Argus,.W Infrageneric classification of Salix L. (Salicaceae) in the New World. Systematic Botany Monographs, 52. Bagchi, S.K Selection differential and predicted genetic gain in Tectonagrandis. Indian Forester, 121(6): Burton,.W Quantitative inheritance in grasses. roceedings of the 6 th International rassland Congress, 1: Burton,.W., Dewane, C.H Estimating heritability in tall fescue (Festucaarundinacea) from replicated clonal material. Agron. J., 45: Dar, U., Kachroo, Alpine flora of Kashmir Himalaya. Scientific publishers, Jodhpur, India, pp Divakara, B.N., Das, R Variability and divergence in ongamiapinnata for further use in tree improvement. J. Forestry Res., 22(2): awali, A., Wagh, R., Sonawane, C Evaluation of genetic variability and correlation in pod and seed traits of ongamiapinnata (L.) ierre. germplasm for genetic tree improvement. Forest Res., 4(3): 1-6. era, N., era, M., urohit, M., Agarwal, R Intra-population variation in Albiziaprocera: od, seed and germination characteristics. Indian Forester, 127(9): inwal, H.S., hartyal, S.S., Rawat,.S., 1606

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