Brassinosteroids Regulate Anthocyanin Biosynthesis in the Ripening of Grape Berries
|
|
- Angel Harper
- 6 years ago
- Views:
Transcription
1 Brssinosteroids Regulte Anthocynin Biosynthesis in the Ripening of Grpe Berries L.-Y. Lun 1, Z.-W. Zhng 1,2*, Z.-M. Xi 1,2*, S.-S. Huo 1 nd L.-N. M 1 (1) College of Enology, Northwest A&F University, Yngling, 7121, Chin (2) Shnxi Engineering Reserch Centre for Viti-Viniculture, Yngling, 7121, Chin Sumitted for puliction:jnury 213 Accepted for puliction:my 213 Key words: Grpe, nthocynin, plnt hormone, rssinosteroid, quntittive-pcr Anthocynins re importnt components in the skins of grpes nd in the development of wine colour. Vrious environmentl fctors cuse poor colortion in some res, even for the sme cultivrs plnted in different production res. The ojective of this study ws to evlute the effect of exogenous rssinosteroids () on the ccumultion of nthocynins nd gene expression of nthocynin iosynthesis in wine grpe erry skins. The results show tht totl nthocynin content in -treted grpes ws higher thn tht in the control () grpes, nd tht.4 mg/l ws the most effective tretment concentrtion. The effect of on downstrem genes ws more effective thn tht on upstrem genes. Full colortion of -treted grpes ws chieved seven dys erlier thn in the cse of. Moreover, enhnced the trnscript level of the downstrem genes of nthocynin iosynthesis, which cused the totl nthocynin content to increse. The induction of structurl nd regultory genes of the flvonoid pthwy suggests tht the interreltionships etween developmentl nd environmentl signlling pthwys were mgnified y tretment, which ctively promoted fruit colortion, nmely nthocynin iosynthesis. INTRODUION Skin colour is one of the most importnt qulities used s sis for selection in reeding progrmmes for wine grpes. As result of nturl hyridistion nd humn selection over millenni, the skin colour of grpes hs ecome gretly diversified, such s lck, red, pink, grey, white, etc. (This et l., 27). Berry colour results from the synthesis nd ccumultion of group of coloured secondry metolites clled nthocynins, nd is determined y the quntity nd composition of nthocynins. Cultivrs with coloured skins ccumulte nthocynins in their skins, wheres whiteskinned cultivrs do not (Boss et l., 1996). With regrd to the wine industry, colour is crucil for qulity in the production of premium red wines. The first sensoril contct with wine is usully mde y visul inspection, which strts uilding up the consumer s perceived qulity. This trnsltes wine colour into vlue of commercil nd economic relevnce (Cstellrin et l., 27). The presence of nthocynins in grpe erries is importnt for the fermenttive processes involved in wine production (Sprvoli et l., 1994), nd they cn interct with some rom sustnces nd influence wine flvour, though nthocynins re odourless nd nerly flvourless (Dufour & Suvitre, 2). As is known, the consumption of nthocynins nd other polyphenols hs helth enefits ssocited with the scvenging of free rdicls nd protective effects ginst crdiovsculr diseses nd so on (Liu, 21). Red grpes nd wines re prticulrly rich in ioville nthocynins, which re rpidly sored s intct molecules (Bitsch et l., 24) nd delivered into the rin within minutes of their ingestion (Pssmonti et l., 25). Therefore colortion is n importnt fctor in determining the qulity of wine grpes (Mori et l., 25). Anthocynins cn e modulted y vriety of environmentl stimuli, including developmentl signls, plnt hormones, nd environmentl stresses such s light, temperture, irrigtion nd so on (Boss & Dvies, 29). All of these things hve dul outcome: on the one hnd, vrious environmentl fctors cuse poor colortion in some res (Mori et l., 25), even for the sme cultivrs plnted in different grpe production res; nd on the other hnd, these environmentl stimuli cn e used to improve the grpe colortion. There hve een mny kinds of clssicl plnt hormones pplied to grpes to improve colortion, such s ethylene, nphthlene cetic cid, nd scisic cid, nd these hve lso een implicted in the control of ripening of grpe erries (Bn et l., 23; El-keremy et l., 23; Jeong et l., 24; Quirog et l., 29). However, very little *Corresponding uthors: E-mil: zhngzhw6@nwsuf.edu.cn; xizhumei@nwsuf.edu.cn Aknowledgements: This study ws supported y the Ntionl Technology System for Grpe Industry (CARS-3-zp-9), the Nturl Science Foundtion of Shnxi Province (211JM34) nd the Scientific Reserch Progrm of Northwest A&F University (QN2959). Our thnks lso go to the Key Lortory of Horticulturl Plnt Biology nd Germplsm Innovtion, Northwest Ministry of Agriculture, Chin 196
2 Brssinosteroids in the Ripening of Grpe Berries 197 is known out how rssinosteroids (s) regulte plnt growth, especilly in grpes. s, considered s the sixth group of hormones in plnts, re steroidl hormones, which re essentil for norml plnt growth nd development such s seed germintion, rhizogenesis, flowering, senescence nd scission, nd they re considered s plnt hormones nd hve pleiotropic effects (Vrdhini & Ro, 22). Symons et l. (26) showed tht the ppliction of to grpe erries evidently enhnced skin colortion nd the finl sugr level of the flesh, nd significntly promoted ripening; on the other hnd, rssinzole (Brz, n inhiitor of iosynthesis) significntly delyed fruit ripening. Peng et l. (211) found tht jsmonte-induced nthocynin ccumultion in Aridopsis seedlings ws reduced y tretment with Brz, wheres it ws enhnced y n ppliction of epirssinolide (epi-bl, the most ctive ). So s re the ltest plnt hormones implicted in the control of erry ripening nd nthocynin ccumultion. However, reports out s influencing the expression of nthocynin iosynthesis genes in grpes re few. In this experiment we therefore investigted the effect of s on nthocynin ccumultion in wine grpes. The pthwy leding to the production of nthocynins cn e divided into two prts, the generl phenylpropnoid pthwy nd the flvonoid pthwy (Fig. 1) (for review, see Boss et l., 1996c; Bogs et l., 26). This pthwy hs een chrcterised in different plnt species (Winkel, 26), especilly in Vitis vinifer (Vv), where the expression of genes involved in flvonoid synthesis (prticulrly nthocynins nd pronthocynidins) hs een well chrcterised in the erries nd seeds of oth red nd white cultivrs (Sprvoli et l., 1994; Boss et l., 1996, 1996; Bogs et l., 26; Cstellrin et l., 27). Recently, two clsses of genes ffecting nthocynin iosynthesis hve een descried. One clss includes the structurl genes of the pthwy, which is common to different species, nd the second clss includes genes tht regulte the ctivity of the iosynthetic genes, conditioning the sptil nd temporl ccumultion of pigments (Sprvoli et l., 1994). It hs een shown tht these regultory genes elong to two mjor groups of trnscription fctors, nmely the MYB nd HLH fmilies (Holton & Cornish, 1995; Sinz et l., 1997; Spelt et l., 2; Ozeki et l., 23; Rmsy et l., 23; Roins et l., 23; Borovsky et l., 24; Schwinn et l., 26). In grpes, My-relted trnscription-fctor genes (such s VlMYBA1-1, VlMYBA1-2 nd VlMYBA2) hve een isolted from mture erries, nd these My-relted genes re involved in the regultion of nthocynin iosynthesis in the grpe vi the expression of the UFGT gene (Koyshi et l., 22). In ddition, VvMYBA1, homolog of VlMYBA1-1 tht is found in mny V. vinifer cultivrs, ws shown to hve the sme function in nthocynin iosynthesis s VlMYBA1-1 (Koyshi et l., 24, 25). Wlker et l. (27) lso found tht the erry colour locus comprised two very similr genes, VvMYBA1 nd VvMYBA2, nd either gene could regulte colour in the grpe erry. Boss et l. (1996, 1996c) showed tht the expression of UFGT ws criticl for nthocynin iosynthesis in grpes. These results point out Generl Phenylpropnoid Pthwy Coumroyl-CoA CHS CHI F3H Chlcones Flvnones Dihydroflvonols DFR Leuconthocynidins LDOX Anthocynidins UFGT Anthocynins F3 H F3 5 H Flvonoid Pthwy FIGURE 1 Key steps in the flvonoid pthwy leding to the synthesis of nthocynins. The enzymes involved in the pthwy re s follows: CHS, chlcone synthse; CHI, chlcone isomerse; F3 H, flvonoid-3 -hydroxylse; F3 5 H, flvonoid- 3,5 -hydroxylse; F3H, flvnone-3β-hydroxylse; DFR, dihydroflvonol-4-reductse; LDOX, leuconthocynidin dioxygense; UFGT, UDP-Glc: flvonoid-3-o-glucosyltrnsferse. the key role of the VvMYBA1 nd VvMYBA2 trnscription fctors tht specificlly regulte the expression of UFGT, which encodes n enzyme responsile for the conversion of nthocynidins to nthocynins. Therefore, MYBA1 nd MYBA2 re essentil for the development of colortion in ripening grpe erries. The genes of nthocynin iosynthesis in grpes hve een cloned, nd detiled informtion cn e found t NCBI, which enled us to study the control mechnism of nthocynin iosynthesis t the mrna level. In this study, rel-time quntittive polymerse chin rection (Q-PCR) ws used to determine the mrna levels of eight structurl genes (CHS, CHI, F3H, F3 H, F3 5 H, DFR, LDOX nd UFGT) nd two regultory genes (MYBA1, MYBA2) of nthocynin iosynthesis precisely, which enled us to compre the mrna levels of ech exmined gene in control () nd erry skins. Thus, the ojective of this study ws to elucidte the effect of exogenous on nthocynin iosynthesis nd ccumultion in the skins of wine grpes during ripening, nd
3 198 Brssinosteroids in the Ripening of Grpe Berries to unrvel how regultes the nthocynin iosynthesis y compring the genes trnscript levels in nd fruit. The results will provide useful informtion for further reserch on the mechnism of regultion of nthocynin iosynthesis, s well s the effects of tretment on grpe nd wine qulities. MATERIALS AND METHODS Tretment with The experimentl vineyrd ws locted in Jingyng county, Xinyng city, in Shnxi Province in Chin. Six-yer-old own-rooted V. vinifer Cernet Suvignon grpevines of similr growth conditions nd crop lod were used in the study. The vines were spced.8 m within rows nd m etween rows, with the row oriented in north-south direction. Vines were trined on verticl shoot positioning system with pir of wires, nd shoots were trimmed mnully twice, etween loom nd vérison, to height of out m. Four tretments, nd three solutions (2,4-epirssinolide, Ruiio, Germny) of.1 mg/l (1),.4 mg/l (2) nd.8 mg/l (3), were pplied to ll clusters of ten vines in rndomised complete lock design with three replictes in seprte vine rows. The solutions were prepred y dissolving.1 mg,.4 mg nd.8 mg in 1 ml 98% ethnol (Snpu, Xin of Chin) nd ringing ech of the three solutions to 1 L with wter. Tween 8 (Bodi, Tinjin of Chin) ws dded s wetting gent t 1 ml/l. The solutions were spry-pplied t 1 ml per cluster with hnd-held spryer until run off, pre-vérison ( ), ensuring tht ll erry surfces were covered. A wter/ethnol/tween 8 spry ws similrly pplied to the clusters on the vines. Bunches t similr stges of development nd t similr positions on the vine were tgged for tretment. Approximtely 1 erries from t lest 1 unches t similr positions were collected t out 12-dy intervls from 2 to 26 July. Once the grpes egn colouring, smpling ws more frequent (once week). The skin, pulp nd seeds were seprted y hnd immeditely, then frozen in liquid nitrogen nd stored t -8 C until use. Determintion of sugr nd nthocynin content Smples for solule sugr mesurements were pressed. The solule sugr ws determined in juice y the stndrd (GB/T ). The corresponding smple of 2 erries ws peeled, nd the skins were immeditely frozen in liquid nitrogen. After eing finely powdered, one liquot of g ws used for nthocynin nlyses, nd one liquot of.3 g ws used for RNA extrction. Totl nthocynins were extrcted ccording to the method of Meng et l. (212). Ech grpe extrct ws diluted with methnol solution nd sornce ws mesured t 53 nm. The nthocynin content (expressed in terms of cynidin-3-glucoside) ws clculted ccording to the method of Tng (29). RNA extrction nd quntifiction of mrna Totl RNA ws extrcted from the grpe skins following the procedure descried in Reid et l. (26). The qulity of RNA ws verified y demonstrtion of intct riosoml nds following grose gel electrophoresis in ddition to the sornce rtios (A26/28) of 1.8 to. For cdna synthesis, one microgrm of totl RNA ws reverse trnscried using the kit of TKR (Dlin, Chin), ccording to the mnufcturer s instructions. Q-PCR ws crried out using the kit of TKR (Dlin, Chin) in iq5 sequence detector (Bio-Rd, Americ), nd the primers were synthesised y Shnghi Sunny Biotechnology Co., Chin. Keeping the totl rection volume s 2 μl: 1 μl SY Premix Ex Tq TM II (2 ) (TKR, Dlin of Chin), 1 μl Forwrd Primer (1 μm), 1 μl Reverse Primer (1 μm), 1 μl cdna, 7 μl dh 2 O. The therml cycling conditions were 95 C for 3 min, followed y 95 C for 5 s nd 6 C for 3 s for 45 cycles, followed y melting cycle from 6 C to 95 C. GAPDH(m) ws chosen s the reference gene, since its expression levels remined constnt throughout the experiments (Reid et l., 26). All smples were mesured in triplicte, nd every run included the GAPDH control for ech smple. Reltive expression ws determined y normlising the vlues to tht of GAPDH. The difference etween the cycle threshold (Ct) of the trget gene nd tht of GAPDH ws used to otin the normlised expression of the trget genes (Bogs et l., 25). Primer pirs for Q-PCR were designed sed on the coding sequences ville in NCBI, nd some were retrieved from the literture. Primer pirs for CHS, F3 H, F3 5 H, DFR nd LDOX were from Sinill et l. (211); primers for F3H were from Cstellrin et l. (27); for UFGT were from Goto-Ymmoto et l. (22); nd for MYBA1 were from Jeong et l. (24). Primers for CHI nd MYBA2 were newly designed to mplify 1 to 2 p gene frgments (Tle 1). Sttisticl nlysis Sttisticl nlyses were conducted with the sttisticl softwre ANOVA (SPSS 16 for Windows), followed y Duncn multiple rnge test to determine significnt differences with regrd to ll prmeters. Vlues were considered sttisticlly significnt t P < 5. RESULTS AND DISCUSSION Accumultion of nthocynins under tretments In this study, the lst time-point efore n ccumultion of sugr is recorded ws tken s the working definition of vérison (Dvies & Böttcher, 29). The vérison of Cernet Suvignon in 211 commenced round 15 July, TABLE 1 Primers for genes of nthocynin iosynthesis newly designed in this work. Gene Sequence Forwrd primer Reverse primer CHI X75963 CACAGCCATCGGAGTGTA TGTGAATAGGCGAC MYBA2 AB97924 CGAGCAGGGTTGAATAGATG ACCCGCAATCAAGGAC
4 Brssinosteroids in the Ripening of Grpe Berries 199 nd ccording to the record of erry colortion in ripening mde y visul inspection, the grpes egn to colour on 26 July. Fig. 2 nd Tle 2 show the effects of the 1, 2 nd 3 tretments on the ccumultion of solule sugr in erry juices during the ripening stge. The ccumultion of sugr strted t vérison, nd it ws enhnced y ll tretments. The ptterns of sugr ccumultion were similr in ll tretments (except 1) (Fig. 2). Before the rpid ccumultion of nthocynins, the sugr content of these tretments ws 1 > 2 > 3; fter tht, the content ws 2 > 3 > 1. At hrvest, the highest sugr content ws mg/l (2), nd the lowest ws mg/l (). Fig. 3 nd Tle 3 show the effects of the 1, 2 nd 3 tretments on the nthocynin ccumultion in the skins of the grpes during the ripening stge. The ccumultion of nthocynins lgged significntly ehind tht of solule sugr; it ws lso enhnced y ll tretments. The ptterns of nthocynin ccumultion were similr in ll tretments, nd the content of these tretments ws 2 > 3 > 1 (Fig. 3). The highest nthocynin content ws 3.91 mg/g (2), nd the lowest ws 3.37 mg/g (). From vérison to 9 August, the speed of sugr ccumultion ws fst, except during the period when nthocynins ccumulted most rpidly ( to ). The rpid ccumultion of nthocynins lgged ehind tht of solule sugr y out 11 dys. From 9 August to hrvest, oth sugr nd nthocynins incresed more slowly. The grpes of were fully coloured red-purple on (the full colortion period) nd fully coloured purple-lck t hrvest. The time when the nthocynins of the 2 nd 3 erries egn to improve significntly ws efore 9 August, wheres tht of 1 ws lter thn tht. So the full colortion period dvnced y seven dys under the 2 tretment, nd y three to four dys under the 3 tretment, s shown in Fig. 3. Dvies nd Böttcher (29) pointed out tht some metolic ctivities, such s the ccumultion of nthocynins in erry skins, commence t vérison, nd the nthocynin ccumultion studied y Cstellrin et l. (27) lso followed the ccumultion of solule solids. In this experiment, the pttern of nthocynin ccumultion ws different, lgging ehind solule sugr ccumultion (vérison) y out 11 dys ecuse of the cloudy nd riny wether during this period. Pigment ccumultion ws delyed when dequte sunlight ws lcking, nd then rpidly incresed when the sunlight ws dequte. Of ll the tretments, the effect of the 2 (.4 mg/l) tretment on the nthocynin content ws the most effective. Also, the 2 tretment not only enhnced the totl nthocynin content, ut full colortion ws chieved seven dys erlier thn in the. Next, the expression of the genes of nthocynin iosynthesis in the 2 nd grpes ws exmined y Q-PCR. 18 Solule sugr (mg/l) Jul15 Jul26 Aug2 Aug9 Aug16 Aug23 Aug31 FIGURE 2 Solule sugr concentrtion in the juice of grpes from the nd tretments. Verticl rs represent stndrd devition. TABLE 2 Sttisticl nlysis of solule sugr concentrtion in the juice of grpes from the nd tretments. Jul 15 Jul 26 Aug 2 Aug 9 Aug 16 Aug 23 Aug ± ± ±1.72 c ± ± 159.± ±6.26 cd ± ±.83 c ± ± ± ± ± ±.45 d 95.6±2.88 d 11.75± ± ±4.52 c 165.4± ±7 c 1.72±.23 d 9±3.23 e 94.5±1.8 d 137.5±2.46 c ±1.65 c 164± ±2.15 cd Notes: Dt re reported s men ± stndrd devition (SD) vlues; different letters ( nd ) within the sme column indicte significnt differences t the 5 level y Duncn s test.
5 2 Brssinosteroids in the Ripening of Grpe Berries Anthocynins (mg/g) Jul15 Jul26 Aug2 Aug9 Aug16 Aug23 Aug31 FIGURE 3 Anthocynin concentrtion (fresh weight) in the skins of grpes from the nd tretments. Verticl rs represent stndrd devition. TABLE 3 Sttisticl nlysis of nthocynin concentrtion in the skins of grpes from the nd tretments. Jul 15 Jul 26 Aug 2 Aug 9 Aug 16 Aug 23 Aug ±2 23±2 d 2.239± ± ±66 c 3.478± ±.114 c 2 23±3 5± ± ± ± ± ± ±1 34±4 c 2.355± ± ±.188 c 3.511± ±44 26±3 5± ± ± ±.15 d 3.21±.137 c 3.369±45 d Notes: Dt were reported s men ± stndrd devition (SD) vlues; different letters ( nd ) within the sme column indicte significnt differences t the 5 level y Duncn s test. Response of structurl nd regultory genes of nthocynin iosynthesis to tretment The expression of eight structurl genes of the flvonoid iosynthesis pthwy (CHS, CHI, F3H, F3 H, F3 5 H, DFR, LDOX, UFGT), which ply role in nthocynin iosynthesis, nd two regultory genes (MYBA1, MYBA2) tht specificlly regulte UFGT expression, were investigted throughout ripening y Q-PCR. All of these genes were expressed t some time during erry development (Fig. 4). In the erries, CHS, CHI, F3 H, DFR nd ANS were ll expressed. F3H nd F3 5 H were expressed t low levels nd UFGT, MYBA1 nd MYBA2 exhiited no expression efore the colour-chnge period ( ). On 26 July, n increse in trnscript level ws oserved for ll the exmined genes (except F3 H), which ws coincident with chnges in the colour of the grpe erries. All the genes were down-regulted fter full colortion (Fig. 4). The expression of genes for nthocynin iosynthesis ws induced y the 2 tretment (Fig. 4). In the erries, the expression of ll exmined genes ws triggered efore vérison. F3H, F3 H nd F3 5 H trnscript levels pprently were enhnced y tretment nd remined higher thn in the erries t ll stges. The expression peks of F3H, F3 H, F3 5 H, DFR, ANS, UFGT, MYBA1 nd MYBA2 dvnced y 14 dys in the erries compred to the erries. The rpid increse of CHI expression hppened on 26 July in, nd on 2 August in, so it ws thought to e rpidly expressed seven dys in dvnce, nd the time of high expression in ws seven dys longer thn in. Menwhile, the expression pek of CHS lso dvnced y seven dys (Fig. 4). The My-relted trnscription-fctor genes in grpes hve een seprted, nd those tht re involved in the regultion of nthocynin iosynthesis vi regulting the expression of UFGT (Koyshi et l., 22). Wlker et l. (27) found tht either MYBA1 or MYBA2 cn regulte colour in grpe erries. In this study, similr expression ptterns were oserved for UFGT, MYBA1 nd MYBA2. The three genes were not expressed efore the colour chnged nd were trnscried when colour ppered in the erries, nd they still hd similr ptterns (two peks) under tretment (Fig. 4). Thus, the two regultory genes hd close reltionship with UFGT. In the iosynthesis process from flvnones to dihydroflvonols, there re rnch pthwys regulted y F3H, F3 H nd F3 5 H (Fig. 1). The genes coding for the enzymes tht regulte the iosynthesis processes efore flvnones in the flvonoid pthwy re clled upstrem genes in this pper, nd genes fter tht re clled downstrem genes. Then, compred with, ll the expression peks of the genes dvnced in the -treted erries; those of the upstrem genes y seven dys, nd those of the downstrem genes y 14 dys, so the effect of on the downstrem genes ws more pronounced. The period of full colortion ws dvnced y out seven dys, similr to the rnge of the upstrem genes (CHI, CHS) in the -treted erries.
6 Brssinosteroids in the Ripening of Grpe Berries 21 Reltive gene expression 2 1 CHS CHI 3. F3H F3'H Reltive gene expression F3'5'H 2 DFR Reltive gene expression 1 ANS UFGT Reltive gene expression 2 1 Reltive gene expression MYBA1 MYBA2 FIGURE 4. Trnscript profiling of structurl genes nd regultory genes of nthocynin iosynthesis in the skins of erries nd treted erries. Verticl rs represent stndrd devition. Different letters ( nd ) indicte significnt differences t the 5 level y Duncn s test.
7 22 Brssinosteroids in the Ripening of Grpe Berries The expression of upstrem genes therefore is very importnt for nthocynin iosynthesis. The rnge of dvncement of full colortion my e limited y tht of the expression of upstrem genes (CHS nd CHI) in -treted erries. regultes nthocynin iosynthesis Peng et l. (211) showed tht ffected jsmonteinduced nthocynin ccumultion y regulting the lte nthocynin iosynthesis genes (DFR, LDOX nd UFGT). In our experiment, pprently lso incresed the expression of the downstrem nthocynin iosynthesis genes. Therefore, nthocynin ccumultion cn e enhnced y up-regulted expression of the downstrem nthocynin iosynthesis genes. CONCLUSIONS This study evluted the effect of exogenous pplied to Cernet Suvignon clusters on the ccumultion of nthocynins nd the gene expression of nthocynin iosynthesis. The 2 tretment (.4 mg/l) significntly incresed the content of solule sugr nd totl nthocynins, nd resulted in full colortion seven dys in dvnce. ffected different tissues of the erry: skin cells (nthocynin ccumultion is restricted to) nd erry flesh cells (sugr is ccumulted in). The effect of on the downstrem genes ws more effective thn on the upstrem genes. The rnges of dvncement of full colortion were limited to the expression of upstrem genes (CHI nd CHS). Moreover, 2 enhnced the trnscript level of the downstrem genes of nthocynin iosynthesis, which cused n increse in totl nthocynin content. Structurl nd regultory genes were induced under the tretment, which suggests tht mgnified the interreltionships etween the developmentl nd environmentl signlling pthwys, which promoted fruit colortion. The results from this study my help to gin n understnding of the control of fruit colortion nd provide informtion for further reserch on the regultion mechnism, s well s on grpe nd wine qulities under the regultion of s. LITERATURE CITED Bn, T., Ishimru, M., Koyshi, S., Shiozki, S., Goto-Ymmoto, N. & Horiuchi, S., 23. Ascisic cid nd 2,4-dichlorophenoxycetic cid ffect the expression of nthocynin iosynthetic pthwy genes in Kyoho grpe erries. J. Hortic. Sci. Biotechnol. 78, Bitsch, R., Netzel, M., Frnk, T., Strss, G. & Bitsch, I., 24. Biovilility nd iokinetics of nthocynins from red grpe juice nd red wine. J. Biomed. Biotechnol Bogs, J., Downey, M., Hrvey, J., Ashton, A., Tnner, G. & Roinson, S., 25. Pronthocynidin synthesis nd expression of genes encoding leuconthocynidin reductse nd nthocynidin reductse in developing grpe erries nd grpevine leves. Plnt Physiol. 139, Bogs, J., Edi, A., Mcdvid, D. & Roinson, S.P., 26. Identifiction of the flvonoid hydroxylses from grpevine nd their regultion during fruit development. Plnt Physiol. 14, Borovsky, Y., Oren-Shmir, M., Ovdi, R., De Jong, W. & Prn, I., 24. The A locus tht controls nthocynin ccumultion in pepper encodes MYB trnscription fctor homologous to Anthocynin2 of Petuni. Theor. Appl. Genet. 19, Boss, P.K. & Dvies, C., 29 (2 nd ed). Moleculr iology of nthocynin ccumultion in grpe erries. In: Rouelkis-Angelkis, K.A. (ed). Grpevine moleculr physiology & iotechnology. Springer-Verlg, Berlin. pp Boss, P.K., Dvies, C. & Roinson, S.P., Expression of nthocynin iosynthesis pthwy genes in red nd white grpes. Plnt Mol. Biol. 32, Boss, P.K., Dvies, C. & Roinson, S.P., Anlysis of the expression of nthocynin pthwy genes in developing Vitis vinifer L cv Shirz grpe erries nd the implictions for pthwy regultion. Plnt Physiol. 111, Boss, P.K., Dvies, C. & Roinson, S.P., 1996c. Anthocynin composition nd nthocynin pthwy gene expression in grpevine sports differing in erry skin colour. Aust. J. Grpe Wine Res. 2, Cstellrin, S.D., Pfeiffer, A., Sivilotti, P., Degn, M., Peterlunger, E. & DI Gspero, G., 27. Trnscriptionl regultion of nthocynin iosynthesis in ripening fruits of grpevine under sesonl wter deficit. Plnt Cell Environ. 3, Cstellrin, S.D., Mtthews, M.A., Di Gspero, G. & Gmett, G.A., 27. Wter deficits ccelerte ripening nd induce chnges in gene expression regulting flvonoid iosynthesis in grpe erries. Plnt 227, Dvies, C. & Böttcher, C., 29 (2 nd ed). Hormonl control of grpe erry ripening. In: Rouelkis-Angelkis, K.A. (ed). Grpevine moleculr physiology & iotechnology. Springer-Verlg, Berlin. pp Dufour, C. & Suvitre, I., 2. Interctions etween nthocynins nd rom sustnces in model system. Effect on the flvor of grpe-derived everges. J. Agric. Food Chem. 48, El-keremy, A., Chervin, C., Roustn, J.P., Cheynier, V., Souquet, J.M., Moutounet, M., Rynl, J., Ford, C., Ltche, A., Pech, J.C. & Bouzyen, M., 23. Exogenous ethylene stimultes the long-term expression of genes relted to nthocynin iosynthesis in grpe erries. Physiol. Plnt 119, GB/T , 26. Anlyticl methods of wine nd fruit wine. Stndrd Press, Beijing, Chin. Goto-Ymmoto, N., Wn, G.H., Mski, K. & Koyshi, S., 22. Structure nd trnscription of three chlcone synthse genes of grpevine (Vitis vinifer). Plnt Sci. 162, Holton, T.A. & Cornish, E.C., Genetics nd iochemistry of nthocynin iosynthesis. The Plnt Cell 7, Jeong, S.T., Goto-Ymmoto, N., Koyshi, S. & Esk, A., 24. Effects of plnt hormones nd shding on the ccumultion of nthocynins nd the expression of nthocynin iosynthetic genes in grpe erry skins. Plnt Sci. 167, Koyshi, S., Goto-Ymmoto, N. & Hirochik, H., 24. Retrotrnsposoninduced muttions in grpe skin color. Science 34, 982. Koyshi, S., Goto-Ymmoto, N. & Hirochik, H., 25. Assocition of VvmyA1 gene expression with nthocynin production in grpe (Vitis vinifer) skin-color mutnts. J. Jpn. Soci. Hortic. Sci. 74, Koyshi, S., Ishimru, M., Hirok, K. & Hond, C., 22. Myrelted genes of the Kyoho grpe (Vitis lruscn) regulte nthocynin iosynthesis. Plnt 215, Liu, H.F., 21. Cloning nd expression of nthocynin iosynthesis relted structure genes in Vitis murensis (in Chinese). Thesis, Northest Forestry University, 26 Hexing Rd, Xingfng, Hrin, Heilongjing, Chin. Meng, J.F., Fng, Y.L., Qin, M.Y., Zhung, X.F. & Zhng, Z.W., 212. Vrietl differences mong the phenolic profiles nd ntioxidnt properties of four cultivrs of spine grpe (Vitis dvidii Foex) in Chongyi County (Chin). Food Chem. 134,
8 Brssinosteroids in the Ripening of Grpe Berries 23 Mori, K., Sugy, S. & Gemm, H., 25. Decresed nthocynin iosynthesis in grpe erries grown under elevted night temperture condition. Sci Hortic-Amsterdm. 15, Ozeki, Y., Chikgw, Y., Kimur, S., Soh, H.C., Med, K., Pornsiriwong, W., Kto, M., Akimoto, H., Oyngi, M., Fukud, T., Kod, T., Itoh, Y., Ymd, A., Dvies, E., Ueno, H. & Tked, J., 23. Puttive cis-elements in the promoter region of the crrot phenyllnine mmoni-lyse gene induced during nthocynin synthesis. J. Plnt Res. 116, Pssmonti, S., Vrhovsek, U., Vnzo, A. & Mttivi, F., 25. Fst ccess of some grpe pigments to the rin. J. Agr. Food Chem. 53, Peng, Z.H., Hn, C.Y., Yun, L.B., Zhng, K., Hung, H.M. & Ren, C.M., 211. Brssinosteroid enhnces jsmonte-induced nthocynin ccumultion in Aridopsis seedlings. J. Integr. Plnt Biol. 53, Quirog, A.M., Berli, F.J., Moreno, D., Cvgnro, J.B. & Bottini, R., 29. Ascisic cid sprys significntly increse yield per plnt in vineyrdgrown wine grpe (Vitis vinifer L.) cv. Cernet Suvignon through incresed erry set with no negtive effects on nthocynin content nd totl polyphenol index of oth juice nd wine. J. Plnt Growth Regul. 28, Rmsy, N., Wlker, A., Mooney, M. & Gry, J., 23. Two sic-helixloop-helix genes (MYC-146 nd GL3) from Aridopsis cn ctivte nthocynin iosynthesis in white-flowered Mtthiol incn mutnt. Plnt Mol. Biol. 52, Reid, K.E., Olsson, N., Schlosser, J., Peng, F. & Lund, S.T., 26. An optimized grpevine RNA isoltion procedure nd sttisticl determintion of reference genes for rel-time RT-PCR during erry development. BMC Plnt Biol. 6, 27. Roins, M., Polocci, F., Hughes, J., Turchetti, V., Allison, G., Arcioni, S., Morris, P. & Dmini, F., 23. Sn, mize HLH gene, modultes nthocynin nd condensed tnnin pthwys in Lotus cornicultus. J. Exp. Bot. 54, Sinz, M., Grotewold, E. & Chndler, V., Evidence for direct ctivtion of n nthocynin promoter y the mize C1 protein nd comprison of DNA inding y relted My domin proteins. Plnt Cell 9, Schwinn, K., Venil, J., Shng, Y., Mcky, S., Alm, V., Butelli, E., Oym, R., Biley, P., Dvies, K. & Mrtin, C., 26. A smll fmily of MYBregultory genes controls florl pigmenttion intensity nd ptterning in the genus Antirrhinum. Plnt Cell 18, Sinill, B., Ovdi, R., Nissim-Levi, A., Perl, A., Crmeli-Weisserg, M. & Oren-Shmir, M., 211. Incresed ccumultion nd decresed ctolism of nthocynins in red grpe cell suspension culture following mgnesium tretment. Plnt 234, Sprvoli, F., Mrtin, C., Scienz, A., Gvzzi, G. & Tonelli, C., Cloning nd moleculr nlysis of structurl genes involved in flvonoid nd stilene iosynthesis in grpe (Vitis vinifer L.). Plnt Mol. Biol. 24, Spelt, C., Quttrocchio, F., Mol, J. & Koes, R., 2. Anthocynin1 of petuni encodes sic helix-loop-helix protein tht directly ctivtes trnscription of structurl nthocynin genes. Plnt Cell 12, Symons, G.M., Dvies, C., Shvrukov, Y., Dry, I.B., Reid, J.B. & Thoms, M.R., 26. Grpes on steroids. Brssinosteroids re involved in grpe erry ripening. Plnt Physiol. 14, Tng, H.L., 29. The qulity nlyzed grpe & wine from origin producing re in MNSi county of XinJing province (in Chinese). Thesis, Northwest A&F University, 26 Hexing Rd, Xingfng, Hrin, Heilongjing, Chin. This, P., Lcome, T., Cdle-Dvidson, M. & Owens, C.L., 27. Wine grpe (Vitis vinifer L.) color ssocites with llelic vrition in the domestiction gene VvmyA1. Theor. Appl. Genet. 114, Vrdhini, B.V. & Ro, S.S.R., 22. Accelertion of ripening of tomto pericrp discs y rssinosteroids. Phytochem. 61, Wlker, A.R., Lee, E., Bogs, J., Mcdvid, D.A., Thoms, M.R. & Roinson, S.P., 27. White grpes rose through the muttion of two similr nd djcent regultory genes. Plnt J. 49, Winkel, B.S.J., 26. The iosynthesis of flvonoids. In: Grotewold, E. (ed). The science of flvonoids. Springer-Verlg, Berlin. pp
Maejo International Journal of Science and Technology
Mejo Int. J. Sci. Technol. 2011, 5(03), 365-373 Communiction Mejo Interntionl Journl of Science nd Technology ISSN 1905-7873 Aville online t www.mijst.mju.c.th Sunlight-stimulted phenyllnine mmoni-lyse
More informationPostharvest Application of 1-Methylcyclopropene (1-MCP) Extends Shelf Life of Kiwifruit
Posthrvest Appliction of 1-Methylcyclopropene (1-MCP) Extends Shelf Life of Kiwifruit C.M. Cntin 1, D. Holcroft 2 nd C.H. Crisosto 1 1 University of Cliforni, Dvis, Deprtment of Plnt Sciences, Dvis, CA
More informationEvaluation of Yogurt with Enhanced Cysteine Content
Diry Foods Evlution of Yogurt with Enhnced Cysteine Content S. Bl nd K. A. Schmidt Summry Amino cids re the uilding locks of protein nd ssist with metolism in the ody. In the humn ody, the mino cid cysteine
More informationEffect of AVG Application on Fruit Set, Yield and Fruit Size in Abate Fetel and Packam s Triumph Pears in a Semi-Commercial Statistical Trial
Effect of AVG Appliction on Fruit Set, Yield nd Fruit Size in Ate Fetel nd Pckm s Triumph Pers in Semi-Commercil Sttisticl Tril E. Sánchez nd M. Curetti Instituto Ncionl de Tecnologí Agropecuri Estción
More informationAsian Journal of Food and Agro-Industry ISSN Available online at
As. J. Food Ag-Ind. 2010, 3(04), 368-372 Asin Journl of Food nd Agro-Industry ISSN 1906-3040 Aville online t www.jofi.info Reserch Article Effect of drying nd frying time on texturl nd sensory chrcteristics
More information2011 ORGANIC SOYBEAN VARIETY TRIAL MATERIALS AND METHODS
2011 ORGANIC SOYBEAN VARIETY TRIAL In 2011, the University of Vermont Extension conducted soyben vriety tril in Alburgh, VT. The purpose of this tril ws to provide yield comprisons of food- nd feed-grde
More informationCluster-zone leaf removal refers to deliberate removal of selected
Lef Removl s Influence on Pinot Noir Bsl lef removl chnges chemicl nd voltile compositions By Hui Feng, Fng Yun, Ptrici A. Skinkis nd Michel C. Qin Cluster-zone lef removl refers to delierte removl of
More informationAcknowledgements. Recent Research: Vine Balance and Fruit Thinning. Brief Outline. Take Aways. How many of you have read? Vine Balance.
Recent Reserch: Vine Blnce nd Fruit Thinning Sustinble Ag Expo Monterey County Jim Wolpert Extension Viticulturist Deprtment of Viticulture nd Enology UC Dvis Acknowledgements Funding Americn Vineyrd Foundtion
More informationInfluence of Gibberellic Acid (GA 3 ) on Fruit Quality of Sweet Cherries John Cline
Influence of Gibberellic Acid (GA 3 ) on Fruit Qulity of Sweet Cherries John Cline University of Guelph, Simcoe & Vinelnd Cmpuses Tel: 519-426-7127 Ext 331 Jcline@uoguelph.c 1 Presenttion Outline Introduction
More informationCentral NC Organic Bell and Hot Pepper Variety Trial, Summer 2014
Ojective Centrl NC Orgnic Bell nd Hot Pepper Vriety Tril, Summer 2014 North Crolin A&T Stte University John Beck, Aron Moore, nd Dr. Snjun Gu NC A&T Frm, Greensoro, NC To evlute the performnce of some
More informationA.M. Jordão *, A.C. Correia
Reltionship Between Antioxidnt Cpcity, Pronthocynidin nd Anthocynin Content During Grpe Mturtion of Tourig Ncionl nd Tint Roriz Grpe Vrieties A.M. Jordão *, A.C. Correi Polytechnic Institute of Viseu,
More informationTiming of partial defoliation affects carbohydrate
SHORT COMMUNICATION Timing of prtil defolition ffects crohydrte concentrtion of vegettive tissues nd concentrtion of phenolics in erries of potted De Chunc (Vitis sp.) grpevines Justine E. Vnden Heuvel
More informationUC DAVIS VITICULTURE AND ENOLOGY EFFECTS OF CULTURAL PRACTICES ON WINEGRAPE COMPOSITION S. KAAN KURTURAL COOPERATIVE EXTENSION SPECIALIST
EFFECTS OF CULTURAL PRACTICES ON WINEGRAPE COMPOSITION S. KAAN KURTURAL COOPERATIVE EXTENSION SPECIALIST Desirle Aspects Uniformly ripe fruit Sound fruit An undnce of flvor With correct composition Reches
More informationOptimized wine quality potential through fruit-zone management practices in red varieties
Finl Progress Report Virgini Wine Bord, 20 July 2016 Optimized wine qulity potentil through fruit-zone mngement prctices in red vrieties Principl Investigtor: Tony K. Wolf AHS Jr. AREC 595 Lurel Grove
More informationThe pear cultivars Spadona and Coscia produce
Journl of Horticulturl Science & Biotechnology (7) 82 () 83 87 Plnt growth regultors increse the fruit size of Spdon nd Cosci pers (Pyrus communis) in wrm climte By RAPHAEL A. STERN 1 *, ISRAEL DORON 2
More informationInduction of ethylene in avocado fruit in response to chilling stress on tree
Journl of Plnt Physiology ] (]]]]) ]]] ]]] www.elsevier.de/jplph Induction of ethylene in vocdo fruit in response to chilling stress on tree Ver Hershkovitz,,, Hy Friedmn, Eliezer E. Goldschmidt, Oleg
More informationResponses of Vitis vinifera Pinot gris Grapevines to Exogenous Abscisic Acid (ABA): I. Yield, Fruit Quality, Dormancy, and Freezing Tolerance
J Plnt Growth Regul (16) 35:245 255 DOI.07/s00344-015-9529-2 Responses of Vitis vinifer Pinot gris Grpevines to Exogenous Ascisic Acid (ABA): I. Yield, Fruit Qulity, Dormncy, nd Freezing Tolernce Shouxin
More informationKucuker and Ozturk Afr J Tradit Complement Altern Med. (2015) 12(2):
Kucuker nd Ozturk Afr J Trdit Complement Altern Med. (2015) 12(2):114-119 http://dx.doi.org/10.4314/jtcm.v12i2.17 THE EFFECTS OF AMINOETHOXYVINYLGLYCINE AND METHYL JASMONATE ON BIOACTIVE COMPOUNDS AND
More informationRapid Reduction in Aroma Volatiles of Pacific Rose Apples in Controlled Atmospheres
Rpid Reduction in Arom Voltiles of Pcific Rose Apples in Controlled Atmospheres H.J. Tough nd E.W. Hewett Centre for Posthrvest nd Refrigertion Reserch Institute of Nturl Resources, Mssey University Plmerston
More informationTHE EXTRACTION KINETICS OF ANTHOCYANINS AND PROANTHOCYANIDINS FROM GRAPE TO WINE IN THREE DIFFERENT VARIETIES
THE EXTRACTION KINETICS OF ANTHOCYANINS AND PROANTHOCYANIDINS FROM GRAPE TO WINE IN THREE DIFFERENT VARIETIES An B. BAUTISTA-ORTÍN 1, Nir BUSSE-VALVERDE 1, José I. FERNÁNDEZ-FERNÁNDEZ 2, Encrn GÓMEZ-PLAZA
More informationThe Relationship Between Palm Oil Index Development and Mechanical Properties in the Ripening Process of Tenera Variety Fresh Fruit Bunches
Reserch Journl of Applied Sciences, Engineering nd Technology 3(3): 218-226, 211 ISSN: 24-7467 Mxwell Scientific Orgniztion, 211 Received: Jnury 31, 211 Accepted: Ferury 23, 211 Pulished: Mrch 3, 211 The
More informationThe Effect of Oat Bran Fiber on Texture, Moisture and Palatability of Pumpkin Muffins
The Effect of Ot Brn Fiber on Texture, Moisture nd Pltbility of Pumpkin Muffins M. Montrsi & K. Reidenbch 11/19/2012 NUTR 453 Finl Report Abstrct: Reserch hd shown n lrmingly low fiber intke of United
More informationApplication of Toasted Oak and Micro-oxygenation to Ageing of Cabernet Sauvignon Wines
Appliction of Tosted Ok nd Micro-oxygention to Ageing of Cernet Suvignon Wines Dr. Jeff McCord StVin Inc., Cliforni, USA Introduction For mny yers, tosted ok in the form of rrels hs een used for wine storge
More informationAbstract. 1. Introduction. Journal of Nutrition & Pharmacy Research
Tomto Fruits (Solncee Lycopersicon esculentum Mill.) Feedck Mechnism in The Presence of Exogenous Ethylene under Prolonged Chilling Temperture Storge M. Mkky,, *, K. A. Pschlidis, P. Soni, A. Mngnris,
More informationColonization of chasmothecia of grapevine powdery mildew by Ampelomyces quisqualis
Biocontrol of powdery mildew Coloniztion of chsmotheci of grpevine powdery mildew y Ampelomyces quisqulis Drio Angeli, Frnc Vlentini, Chir Msiero, Oscr Giovnnini, Ilri Pertot Fondzione Edmund Mch, S.Michele
More informationEVALUATION OF SEED AND IN-FURROW AT-PLANTING APPLIED INSECTICIDES ON SORGHUM
EVALUATION OF SEED AND IN-FURROW AT-PLANTING APPLIED INSECTICIDES ON SORGHUM Texs Agriculturl Experiment Sttion, Corpus Christi, TX, nd Michel nd Wlter Kuck Frm, Lvc County, 1999 Roy D. Prker, Emil D.
More informationScienceDirect. Development of a method for manufacturing noodles from finger millet
Avilble online t www.sciencedirect.com ScienceDirect Procedi Food Science 6 (2016 ) 293 297 Interntionl Conference of Sbrgmuw University of Sri Lnk 2015 (ICSUSL 2015) Development of method for mnufcturing
More informationEffects of Prohexadione-calcium on Grape Yield Components and Fruit and Wine Composition
Effects of Prohexdione-C on Yield nd Composition 73 Effects of Prohexdione-clcium on Grpe Yield Components nd Fruit nd Wine Composition Dnielle Lo Giudice, 1 Tony K. Wolf, 2 * nd Bruce W. Zoecklein 3 Abstrct:
More informationThe Impact of Supplementation of Green Tea Powder in Yellow Cupcakes. Teryn Sapper & Christian Ordaz November 22, 2010 F&N 453
The Impct of Supplementtion of Green Te Powder in Yellow Cupckes Teryn Spper & Christin Ordz Novemer 22, 2010 F&N 453 Astrct: Green te is known to hve mny helth enefits to the humn ody. Cumultive studies
More informationArchives 2016 vol *
August 30, 2016 Archives 2016 vol.1 1-9 EVALUATION OF THE ANTIOXIDANT CAPACITY AND CONTENT OF POLYPHENOLS OBTAINED FROM TEA (Cmelli Sinensis) OF FOUR BRANDS SOLD IN COLOMBIA BY EXTRACTION AT ROOM TEMPERATURE
More informationProduction of Two Types of Pocket-Forming Flat Bread by the Sponge and Dough Method
Production of Two Types of Pocket-Forming Flt Bred y the Sponge nd Dough Method A. Amr 1,2 nd R. Ajo 1 ABSTRACT Cerel Chem. 82(5):499 503 Two types of flt red (thin nd thick) were produced from strightgrde
More information2014 Sunflower Planting Date Trial
214 Sunflower Plnting Dte Tril Dr. Hether Drby, UVM Extension Agronomist Sr Ziegler, Eric Cummings, Susn Monhn, nd Julin Post UVM Extension Crops nd Soils Technicins (82) 524-651 Visit us on the web t
More informationEarly-Maturing Sweet Oranges: Research Update on Earlygold, Itaborai, Ruby, and Westin Sweet Oranges 1
HS920 Erly-Mturing Sweet Ornges: Reserch Updte on Erlygold, Itori, Ruy, nd Westin Sweet Ornges 1 Willim S. Cstle nd Jmes J. Ferguson 2 Erlygold, Itori, Ruy, nd Westin re new sweet ornge selections with
More informationResearch Note Effect of Volume and Toast Level of French Oak Barrels (Quercus petraea L.) on Cabernet Sauvignon Wine Characteristics
Reserch Note Effect of Volume nd Tost Level of French Ok Brrels (Quercus petre L.) on Cernet Suvignon Wine Chrcteristics Pedro Rodríguez-Rodríguez 1 nd Encrn Gómez-Plz 1 * Astrct: The effects of tost level
More informationRefining Fruit-Zone Leaf Removal for Red-Fruited Bordeaux Varieties Grown in a Humid Environment. Cain Charles Hickey
Refining Fruit-Zone Lef Removl for Red-Fruited Bordeux Vrieties Grown in Humid Environment. Cin Chrles Hickey Disserttion sumitted to the fculty of the Virgini Polytechnic Institute nd Stte University
More informationPostharvest quality and chilling injury of plums: benefits of 1-methylcyclopropene
Instituto Ncionl de Investigción y Tecnologí Agrri y Alimentri (INIA) Spnish Journl of Agriculturl Reserch 9(), 554-564 Aville online t www.ini.es/sjr ISSN: 695-97-X eissn: 7-99 Posthrvest qulity nd chilling
More informationSeparation of Sunlight and Temperature Effects on the Composition of Vitis vinifera cv. Merlot Berries
Sunlight nd Temperture Effects on Merlot Berries 171 Seprtion of Sunlight nd Temperture Effects on the Composition of Vitis vinifer cv. Merlot Berries S.E. Spyd, 1 * J.M. Trr, 2 D.L. Mee, 1 nd J.C. Ferguson
More informationResearch Note Relationship between Skin Break Force and Anthocyanin Extractability at Different Ripening Stages
Reserch Note Reltionship etween Skin Brek Force nd Anthocynin Extrctility t Different Ripening Stges Luc Rolle, 1 * Frizio Torchio, 2 Giuseppe Zepp, 1 nd Vincenzo Geri 3 Astrct: The extrctility of nthocynins
More informationINTERNATIONAL JOURNAL OF FOOD ENGINEERING
INTERNATIONAL JOURNAL OF FOOD ENGINEERING Discrimintion of Wines Produced from Cbernet Suvignon Grpes Treted with Aqueous Ethnol Post-Bloom Using n Electronic Nose Amnd Mrtin 1, Kumr Mllikrjun, 2 nd Bruce
More informationEffect of Ultra-high Pressure Treatment on the Chemical Properties, Colour and Sensory Quality of Young Red Wine
Effect of Ultr-high Pressure Tretment on the Chemicl Properties, Colour nd Sensory Qulity of Young Red Wine X. Sun#, X. Chen#, L. Li, T. M, F. Zho, W. Hung, J. Zhn* College of Food Science nd Nutritionl
More informationRESPONSE OF SUNFLOWER HYBRIDS TO DIFFERENT NITROGEN LEVELS FOR PHYSIOLOGICAL AND AGRONOMICAL TRAITS UNDER FIELD CONDITIONS
Pkistn J. Agric. Res. Vol. 9 No1, 016 RESPONSE OF SUNFLOWER HYBRIDS TO DIFFERENT NITROGEN LEVELS FOR PHYSIOLOGICAL AND AGRONOMICAL TRAITS UNDER FIELD CONDITIONS Doult Big*, Fid Mohmmd Asi*, Hi Ahmed*,
More informationThe effect of seed treatments on the yield and yield components of various levels of sprouted wheat
Brnrd nd Booyse (2018). Seed Science nd Technology, 46, 1, 53-63. https://doi.org/10.15258/sst.2018.46.1.05 The effect of seed tretments on the yield nd yield components of vrious levels of sprouted whet
More informationAbstract. Ramiréz-Aristizabal, L. S. * ; Ortiz, A. ; Restrepo-Aristizabal, M.F.; Salinas-Villada, J.F. Universidad Tecnológica de Pereira,Colombia
Evlution of the ntioxidnt cpcity nd content of polyphenols obtined from te (Cmelli sinensis) of four brnds sold in Colombi by extrction t room temperture Rmiréz-Aristizbl, L. S. * ; Ortiz, A. ; Restrepo-Aristizbl,
More informationControl of black dot in potatoes
Control of lck dot in pottoes Dr. Trevor Wicks SA Reserch & Development Institute Project Numer: PT11 PT11 This report is pulished y Horticulture Austrli Ltd to pss on informtion concerning horticulturl
More informationTHE EFFECTS OF BERRY THINNING AND GIBBERELLIN ON RECEL UZUMU TABLE GRAPES
Perfor. of Summer forge legumes in Pothowr Pk. J. Agri. Sci., Vol. 49(2), 105-112; 2012 ISSN (Print) 0552-9034, ISSN (Online) 2076-0906 http://www.pkjs.com.pk THE EFFECTS OF BERRY THINNING AND GIBBERELLIN
More informationGibberellic Acid (GA3), an Influential Growth Regulator for Physiological Disorder Control and Protracting the Harvesting Season of Sweet Orange
Americn Journl of Experimentl Agriculture 4(11): 1355-1366, 2014 SCIENCEDOMAIN interntionl www.sciencedomin.org Gierellic Acid (GA3), n Influentil Growth Regultor for Physiologicl Disorder Control nd Protrcting
More informationSemi-commercial evaluation of SmartFresh with South African export avocados in static containers at the Westfalia packhouse during 2002
South Africn Avocdo Growers' Assocition Yerbook. 2003. 26:55-64 Semi-commercil evlution of SmrtFresh with South Africn export vocdos in sttic continers t the Westfli pckhouse during 2002 D Lemmer #, J
More informationTHE EFFECTS OF POSTHARVEST TREATMENTS OF SALICYLIC ACID AND POTASSIUM PERMANGANATE ON THE STORAGE OF KIWIFRUIT
76 Bulgrin Journl of Agriculturl Science, 6 (No ), 76-8 Agriculturl Acdemy THE EFFECTS OF POSTHARVEST TREATMENTS OF SALICYLIC ACID AND POTASSIUM PERMANGANATE ON THE STORAGE OF KIWIFRUIT E. BAL * nd S.
More informationPREPARATION AND HANDLING FRESH-CUT ROOT VEGETABLES. MERETE EDELENBOS DEPT. OF FOOD SCIENCE AARHUS UNIVERSITY DENMARK
PREPARATION AND HANDLING FRESH-CUT ROOT VEGETABLES MERETE EDELENBOS DEPT. OF FOOD SCIENCE AARHUS UNIVERSITY DENMARK merete.edelenos@food.u.dk Preprtion nd hndling fresh-cut root vegetles Production Hndling
More informationEffects of chemical treatments on dormancy breaking and some sprouting characteristics of two potato cultivars in different tuber sizes
Avilble online t www.pelgireserchlibrry.com Europen Journl of Experimentl Biology, 2014, 4(4):98-102 ISSN: 2248 9215 CODEN (USA): EJEBAU Effects of chemicl tretments on dormncy breking nd some sprouting
More informationArchived at
Archived t http://orgprints.org/13657/ Results from three yer testing project of new strwberry cultivrs in Verticillium infested soils nd under orgnic frming conditions A. Spornberger 1, H. Weissinger
More informationRelationship Between Microclimatic Data, Aroma Component Concentrations and Wine Quality Parameters in the Prediction of Sauvignon blanc Wine Quality
Reltionship Between Microclimtic Dt, Arom omponent oncentrtions nd Wine Qulity Prmeters in the Prediction of Suvignon blnc Wine Qulity J. Mris, F. litz 2 nd P. D. Hsbroek3 1) AR lnfruiiec-nietvoorbij,
More informationTowards Grower-friendly Apple Crop Thinning by Tree Shading
314 Towrs Grower-frienly Apple Crop Thinning y Tree A. Wimer 1, K. Kockerols 1, S. Schwn 1, W. Stler 1 n L. Bertschinger 1 Astrct Light mngement with shing nets, which reuce sunlight y 74%, might e n lterntive
More informationEffects of guar gum and arabic gum on the physicochemical, sensory and flow behaviour characteristics of frozen yoghurt
doi: 1.1111/j.1471-37.211.75.x ORIGINAL RESEARCH Effects of gur gum nd ric gum on the physicochemicl, sensory nd flow ehviour chrcteristics of frozen yoghurt RAHIL REZAEI, MORTEZA KHOMEIRI,* MAHDI KASHANINEJAD
More informationThe Effect of Quinoa on the Taste and Texture of Chocolate Chip Cookies. Hannah Doren Tarryn Hake Mitch Simmonds NUTR 453.
The Effect of Quino on the Tste nd Texture of Chocolte Chip Cookies Hnnh Doren Trryn Hke Mitch Simmonds NUTR 453 November 19, 2012 Title: The Effect of Quino on the Tste nd Texture of Chocolte Chip Cookies
More informationInfluence of Fermentation Temperature on Composition and Sensory Properties of Semillon and Shiraz Wines
Influence of Fermenttion on Properties of Semillon nd Shirz Wines 235 Influence of Fermenttion Temperture on Composition nd Sensory Properties of Semillon nd Shirz Wines Andrew Reynolds, 1* Mrgret Cliff,
More informationHow to get the best eating quality of pork
The effect of ft on the eting qulity of pork Ensuring the best eting qulity Ensuring the best flvours The effect of cooking ft on the eting qulity of pork How to get the best eting qulity of pork The cooking
More informationFood Research International
Food Reserch Interntionl 43 (2010) 2284 2288 Contents lists vilble t ScienceDirect Food Reserch Interntionl journl homepge: www.elsevier.com/locte/foodres Effect of honey powder on dough rheology nd bred
More informationEnsiling characteristics and aerobic stability of temperate grasses containing different concentrations of water soluble carbohydrates
REPORT TO THE STAPLEDON MEMORIAL TRUST Ensiling chrcteristics nd eroic stility of temperte grsses contining different concentrtions of wter solule crohydrtes Aner A. Rodríguez-Crís Deprtment of Animl Science,
More informationPHYSIOLOGY AND POSTHARVEST BEHAVIOUR OF MANGO (Mangifera indica L. cv. TOMMY ATKINS) FRUIT GROWN UNDER WATER STRESS NANCY ONJEMO MADIGU
PHYSIOLOGY AND POSTHARVEST BEHAVIOUR OF MANGO (Mngifer indic L. cv. TOMMY ATKINS) FRUIT GROWN UNDER WATER STRESS NANCY ONJEMO MADIGU MASTER OF SCIENCE (Food Science nd Posthrvest Technology) JOMO KENYATTA
More informationEFFECT OF HARVEST TIME AND L-CYSTEINE AS AN ANTIOXIDANT ON FLESH BROWNING OF FRESH-CUT CHERIMOYA (Annona cherimola Mill.)
RESEARCH EFFECT OF HARVEST TIME AND L-CYSTEINE AS AN ANTIOXIDANT ON FLESH BROWNING OF FRESH-CUT CHERIMOYA (Annon cherimol Mill.) Reinldo Cmpos-Vrgs 1,*, Bruno G. Defilippi 1,, Pul Romero Q. 2, Héctor Vldés
More informationSpray to Flowering Mango Trees on Fruit Retention, Fruit Size, Tree Yield, and Fruit Quality.
Effect of KN 3 Spry to Flowering Mngo Trees on Fruit Retention, Fruit Size, Tree Yield, nd Fruit Qulity. Steven. Oosthuyse Merenslry Tuhno!ogim! 5 ervicej, P.. ox 14, Duive!sk!oof 835, South fric In South
More informationPerformance of Seyval Blanc Grape in Four Training Systems Over Five Years
CROP PRODUCTON HORTSCENCE 37(7): 1023-1027.2002. Performnce of Seyvl Blnc Grpe in Four Trining Systems Over Five Yers D. Ferree, T. Steiner, J. Gllnder, D. Scurlock, G. Johns, nd R. Riesen Deprtment of
More informationPreview. Chapter 3. Labor Productivity and Comparative Advantage: The Ricardian Model
hpter 3 Lbor roductivity nd omprtive Advntge: The Ricrdin Model review Opportunity costs nd comprtive dvntge roduction possibilities Reltive supply, reltive demnd & reltive prices Trde possibilities nd
More informationGarlic Sprouts Grown Indoors at Kitchen Sites
Medicinl nd Aromtic Plnt Science nd Biotechnology 008 Globl Science Books Grlic Sprouts Grown Indoors t Kitchen Sites FeiFei Qin,,3* HuiLin Xu Gng M 4 Interntionl Nture Frming Reserch Center, Ht, Ngno,
More informationThe effects of UV-C treatment on the quality of orange, carrot and celery juice blend
Review Article http://www.lliedcdemies.org/journl-food-science-nutrition/ The effects of UV-C tretment on the qulity of ornge, crrot nd celery juice blend Nik Nornis, Chndrn Somsundrm*, Zulin Rzli Biotechnology
More informationComparison of alternative postharvest quarantine treatments for sweet cherries
Posthrvest Biology nd Technology 20 (2000) 107 114 www.elsevier.com/locte/posthrvio Comprison of lterntive posthrvest qurntine tretments for sweet cherries Lis G. Neven, *, Stephen R. Drke Ykim Agriculturl
More informationEffect of oak barrel type on the volatile composition of wine: Storage time optimization
LWT 39 (26) 199 25 www.elsevier.com/locte/lwt Effect of ok rrel type on the voltile composition of wine: Storge time optimiztion Teres Grde Cerd n, Crmen Ancı n-azpilicuet Deprtment of Applied Chemistry,
More informationThe Effect of Acetic and Lactic Acid on the Oil Uptake, Texture and Color of Rice (Sang Tarom) During Cooking
World Applied Sciences Journl 4 (2): 183-187, 2008 ISSN 1818-4952 IDOSI Publictions, 2008 The Effect of Acetic nd Lctic Acid on the Oil Uptke, Texture nd Color of Rice (Sng Trom) During Cooking 1 1 2 E.
More informationOptimization of extraction of phenolic antioxidants from peanut skins
Journl of the Science of Food nd Agriculture J Sci Food Agric 85:33 38 (2005) DOI: 102/jsf.1933 Optimiztion of extrction of phenolic ntioxidnts from penut skins Vleri Nepote, 1 Nelson R Grosso 2 nd Crlos
More informationTemporal variation in temperature and rainfall differentially affects ectomycorrhizal colonization at two contrasting sites
New Phytol. (1998), 139, 733 739 Temporl vrition in temperture nd rinfll differentilly ffects ectomycorrhizl coloniztion t two contrsting sites BY RANDY L. SWATY*, CATHERINE A. GEHRING, MATT VAN ERT, TAD
More informationGrowth and yield of three sunflower hybrids cultivated for two years under mediterranean conditions
Emirtes Journl of Food nd Agriculture. 2016. 28(2): 136-142 doi: 10.9755/ejf.2015-05-291 http://www.ejf.me/ REGULAR ARTICLE Growth nd yield of three sunflower hyrids cultivted for two yers under mediterrnen
More informationInfluence of some safety post-harvest treatments on fruit quality and storability of Guava fruits
Current Science Interntionl Volume : 06 Issue : 03 July-Sept. 2017 Pges: 491-500 Influence of some sfety post-hrvest tretments on fruit qulity nd storility of Guv fruits 1 Ftm K. M. Shn nd 2 Ahmed M. S.
More information2006 BELL PEPPER VARIETY EVALUATION TRIALS
26 BELL PEPPER VARIETY EVALUATION TRIALS In Sn Joquin County University of Cliforni Coopertive Extension 42 South Wilson Wy Stockton, CA 9525 1 26 BELL PEPPER VARIETY EVALUATION TRIAL In Sn Joquin County
More informationIndicate (X) client(s) to whom this final report is submitted. Replace any of these with other relevant clients if required. FINAL REPORT FOR 2011/12
Finl report 1 CFPA Cnning Fruit Producers Assoc. Sumit to: Wiehhn Victor Tel: +27 (0)21 872 1501 inmk@mwe.co.z SAAPPA / SASPA / SAT Fruitgro Science Sumit to: Louise Lieenerg Tel: +27 (0)21 882 8470/1
More informationEFFECT OF BORON AND ARSENIC ON JUICE ACIDITY
SMITH: BORON & ARSENIC ON GRAPEFRUIT 95 Fig. 2. Vlenci ornge leves from declining tree showing type of mteril used for electron microscopic study Ar rows point to typicl res long midveins tht were excised,
More informationResearch Article Oenological and Quality Characteristic on Young White Wines (Sauvignon Blanc): Effects of High Hydrostatic Pressure Processing
Hindwi Journl of Food Qulity Volume 2017, rticle ID 8524073, 12 pges https://doi.org/10.1155/2017/8524073 Reserch rticle Oenologicl nd Qulity Chrcteristic on Young White Wines (Suvignon Blnc): Effects
More informationEffects of Vine Defoliation on Pinot noir Fruit and Wine Quality
Effects of Vine Defolition on Pinot noir Fruit nd Wine Qulity Jessic Howe Sndrock nd Anit Nin Azrenko Deprtment of Horticulture, Oregon Stte University Industry Collbortors Leigh Bm1holomew, Archery Summit
More informationIR-4 Ornamental Horticulture Program Research Report Cover Sheet
013094.pdf IR4 Ornmentl Horticulture Progrm Reserch Report over Sheet Resercher(s) Gry hstgner Tril: 013WA Affilition: Wshington Stte University Pullyup WA PhoneNumer: (53) 445458 Emil: chstg@wsu.edu ProjectTitle:
More informationAgronomic approaches in yield and quality stability of high oleic sunflowers (Helianthus annuus L.)
Agronomic pproches in yield nd qulity stbility of high oleic sunflowers (Helinthus nnuus L.) to obtin the Ph. D. degree in the Fculty of Agriculturl Sciences, Georg-August-University Göttingen, Germny
More informationVolume 2, ISSN (Online), Published at:
SEASONAL VARIATION OF THE PHYSICAL AND CHEMICAL PARAMETERS OF WILD GENOTYPES OF CORNELIAN CHERRY (Cornus ms L.) Aldin Islmovic 1, Merjem Mlco 1, Ndir Berbic 1, Asim Begic-Akgic 1, Snj Orucevic 1, Amel
More informationInfluence of Soil Salinity on Sensory Characteristics and Volatile Aroma Compounds of Nero d Avola Wine
Influence of Soil Slinity on Sensory Chrcteristics nd Voltile Arom Compounds of Nero d Avol Wine Antonio Sccco, 1 Antonell Verzer, 2 * Crmel M. Lnz, 1 Antonio Sprcio, 3 Giuseppe Genn, 3 Slvtore Rimondi,
More informationFINAL REPORT. August 15, Virginia Wine Board. Effects of Harvest Maturity and Post-Harvest Storage on Fruit, Juice, and Cider Quality
FINAL REPORT August 15, 2016 Virgini Wine Bord Effets of Hrvest Mturity nd Post-Hrvest Storge on Fruit, Juie, nd Cider Qulity Prinipl Investigtors (Tem projet): Gregory M. Pek (50%), Assistnt Professor
More informationFlavonoids in grapes. Simon Robinson, Mandy Walker, Rachel Kilmister and Mark Downey. ASVO SEMINAR : MILDURA, 24 July 2014 AGRICULTURE FLAGSHIP
Flavonoids in grapes Simon Robinson, Mandy Walker, Rachel Kilmister and Mark Downey ASVO SEMINAR : MILDURA, 24 July 2014 AGRICULTURE FLAGSHIP Flavonoids in grapes Grape Flavonoids Flavonoids are important
More informationThe effect of storage conditions on coffee seed and seedling quality
COFFEE SEEDLING PRODUCTION FROM STORED SEEDS Ros, S.D.V.F. d, Crvlho,.M., McDonld, M.., Von Pinho, E.R.V., Silv,.P. nd Veig,.D. (2011), Seed Sci. & Technol., 39, 151-164 The effect of storge conditions
More informationCERTIFIED CLONE AND POWDERY MILDEW IMPACT ROTUNDONE IN DURAS WINES
Olivier GEFFROY *, Olivier YOBRÉGAT, Thierry DUFOURCQ, Trcey SIEBERT,Éric SERRANO IFV Pôle Sud-Ouest, V innopôle, BP, 80 Lisle sur Trn Frnce IFV Pôle Sud-Ouest, Domine de Mons, 00 Cussens Frnce The Austrlin
More informationTechnical Brief In situ Measurements of Dissolved Oxygen during Low-Level Oxygenation in Red Wines
Technicl Brief In situ Mesurements of Dissolved Oxygen during Low-Level Oxygention in Red Wines V. Felipe Lurie, 1 Robert Lw, 2 Willy S. Joslin, 2 nd Andrew L. Wterhouse 3 * Abstrct: Dissolved oxygen ws
More informationReview of Arabica Coffee Management Research in Ethiopia
Review of Aric Coffee Mngement Reserch in Ethiopi Anteneh Netsere 1, Tye Kuf 1 Tesfye Shimer 2 1, Jimm Agriculturl Reserch Center, P.. Box 192, Jimm, Ethopi 2, Ethopin Institute of Agriculturl Reserch,
More informationPruning affects the vegetative balance of the wine grape (Vitis vinifera L.)
crop phisiology Pruning ffects the vegettive lnce of the wine grpe (Vitis vinifer L.) L pod fect el equilirio vegettivo de l vid (Vitis vinifer L.) Pedro José Almnz-Merchán 1, Plo Antonio Serrno-Cely 2,
More informationModeling Impacts of Viticultural and Environmental Factors on 3-Isobutyl-2-Methoxypyrazine in Cabernet franc Grapes
Modeling Impcts of Viticulturl nd Environmentl Fctors on 3-Isobutyl-2-Methoxypyrzine in Cbernet frnc Grpes Justin J. Scheiner, 1 Justine E. Vnden Heuvel, 2 Bruce Pn, 3 nd Gvin L. Scks 4 * Abstrct: A study
More informationOF THE CITRUS RUST MITE
esults of this experiment re in generl greement with the results of our previous experiment (7) tht were otined under the sme grove conditions with different smple loctions. However, different grove nd
More informationAtmospheric CO2 Levels
Adpting to Chnging Climte: Forges for Drought Prone Conditions Chris Teutsch Southern Piedmont AREC Blckstone, VA Atmospheric CO2 Levels 1 Temperture Chnge Hnsen, J.E., R. Ruedy, Mki. Sto, M. Imhoff, W.
More informationMACLIT INDICATOR PILOT LAMP & INDICATOR FEATURES SPECIFICATIONS (RATINGS, PERFORMANCE)
MACLIT INDICATO INDICATO FEATUES The Mclit indictor is operted y the electromgnetic polrity inversion method. The sttus is displyed y rotting the disply prt. The SS nd MS types hve uilt-in LED for night
More informationEFFECT OF FOLIAR NITROGEN AND SULPHUR APPLICATION ON AROMATIC EXPRESSION OF VITIS VINIFERA L. cv. SAUVIGNON BLANC
EFFECT OF FOLIAR NITROGEN AND SULPHUR APPLICATION ON AROMATIC EXPRESSION OF VITIS VINIFERA L. cv. SAUVIGNON BLANC F. LACROUX 1, O. TREGOAT 2, C. VAN LEEUWEN 1, A. PONS 3, T. TOMINAGA 3, Vlérie LAVIGNE-CRUÈGE
More informationEffect of Phosphate Salts on the Pasting Properties of Korean Instant-Fried Noodle
Effect of Phosphte Slts on the Psting Properties of Koren Instnt-Fried Noodle Li Wng, 1,2 Gry G. Hou, 2,3 Y-Hsun Hsu, 2 nd Lirong Zhou 4 ABSTRACT Cerel Chem. 88(2):142 146 Up to 15% modified potto strch
More informationThe photosynthesis inhibitor metamitron is a highly effective thinner for Golden Delicious apple in a warm climate
Fruits, 215, vol. 7(3), p. 127-134 c Cird / EDP Sciences 215 DOI: 1.151/fruits/2157 Aville online t: www.fruits-journl.org Originl rticle The photosynthesis inhiitor metmitron is highly effective thinner
More informationFlavonoids in grapes. Simon Robinson, Mandy Walker, Rachel Kilmister and Mark Downey. 11 June 2014 PLANT INDUSTRY
Flavonoids in grapes Simon Robinson, Mandy Walker, Rachel Kilmister and Mark Downey 11 June 2014 PLANT INDUSTRY Grapes to wine a 2 metabolic zoo Grapevines Hundreds of different metabolites determine Wine
More informationBank of Japan Review. Innovation in Retail Payment Services in Major Economies. November Introduction 2014-E-3
Bnk of Jpn Review 2014-E-3 Innovtion in Retil Pyment Services in Mjor Economies Retil Pyment Systems Group Pyment nd Settlement Systems Deprtment Novemer 2014 Mjor economies hve emrked on vrious innovtive
More informationRudd Report. Are Fast-Food Restaurants Keeping Their Promises to Offer Healthier Kids Meals? Summary. August 2017
Rudd Report UCONNRUDDCENTER.ORG August 2017 Are Fst-Food Resturnts Keeping Their Promises to Offer Helthier Kids Mels? AUTHORS: Jennifer Hrris, PhD, MBA Mi Hyry, MPA Nicole Seymour, MSW Yoon-Young Choi,
More informationInfluence of Citrus Rootstocks in Bioactive Compounds of Clementines
Journl of Food nd Nutrition Reserch, 2017, Vol. 5, No. 8, 545-552 Avilble online t http://pubs.sciepub.com/jfnr/5/8/3 Science nd Eduction Publishing DOI:10.12691/jfnr-5-8-3 Influence of Citrus s in Bioctive
More information