SHORT TERM SCIENTIFIC MISSIONS (STSMs)
|
|
- Harry Gardner
- 6 years ago
- Views:
Transcription
1 SHORT TERM SCIENTIFIC MISSIONS (STSMs) Reference: Short Term Scientific Mission, COST Action FA1003 Beneficiary: Bocharova Valeriia, National Scientific Center Institute of viticulture and winemaking named after V.Ye. Tairov Host: Regner Ferdinand, HBLAuBA Klosterneuburg Period: from to Place: Langenzersdorf (Austria) Reference code: COST-STSM-ECOST-STSM-FA Amount: EUR 1500 I. Purpose of the STSM: STSM Scientific Report The main aim of a short-term training in a HBLAuBA Klosterneuburg laboratory is a possibility to possess the new methods of molecular genetic analysis, get to know about new equipment and research strategies for studying grape genome which was selected on the base of NSC Tairov institute of viticulture and wine-making. In addition, establishing the missing scientific and educational relations between NSC Tairov institute of viticulture and winemaking and Department of Grapevine Breeding with possibility of cooperation in future. Besides, increasing the knowledge about: SSR fingerprinting and the other methods for genetic characterization, phenotyping and mapping, to become proficient in chloroplast typing and other PCR markers for characterization. Learning to use a new sequencing machine Li- Cor 4300 DNA Analyser. The knowledge will give the possibility to provide a research of grapevine genome rate on sufficiently high level. II. Description of the work carried out during the STSM: The first day of DNA extraction from cane samples of cultivars and clones of grapevine (10 samples); Samples list:
2 Aligote 101 clone Cabernet Sauvignon clone Sukcholimansky beliy Odessky cherniy Cabernet Franc Smena Tairian Zagrey Jantar Aromatniy Muskat odesskiy Clones breeding by NSC Tairov institute of viticulture and wine-making Varieties breeding NSC Tairov institute of viticulture and wine-making propagate in Ukraine Varieties propagate in Ukraine New promising varieties breeding NSC Tairov institute of viticulture and winemaking The eleventh sample was brought as extracted DNA from NSC Tairov institute of viticulture and wine-making department of Molecular-genetic and Phytopathology (Muskat odesskiy) The second day of DNA extraction of cultivars and clones of grapevine; Checking the concentration of DNA in agarose gel; Dilution of DNA samples to the desired concentration; The first PCR by SSR-locus VVS (control PCR); Preparation of sequencing gel; Preparation samples for sequencing, pre-run; Sequencing by LI-COR Biosciences 4300 DNA Analyzer (SSR-locus VVS); The second PCR by SSR-loci VVMD7,VVMD7, VVMD5, VRZAG6, VRZAG79; Sequencing by LI-COR Biosciences 4300 DNA Analyzer (SSR-loci VVMD7,VVMD7, VVMD5, VRZAG6, VRZAG79); Learning to work in the program SAGA Generation ; The third PCR by SSR-loci VVMD5,VVMD8, VVMD3; Sequencing by LI-COR Biosciences 4300 DNA Analyzer (SSR-loci VVMD5,VVMD8, VVMD3 ); Data analysis;
3 Sequencing by LI-COR Biosciences 4300 DNA Analyzer; The PCR by chloroplast marker ccmp10; Preparation of gel for silver staining; Data analysis, statistical analysis; Sequencing by LI-COR Biosciences 4300 DNA Analyzer (final results); Preparation of gel for silver staining; Data analysis; PAGE electrophoresis by chloroplast DNA-marker ccmp10 of 10 samples; Silver staining; Data analysis; Beginning of cloning PCR products with pgem-t and pgem-t easy vectors; The extraction of nucleic acid from agarose gels; Ligation Using the purified PCR fragments; Data analysis; III. Description of the main results obtained: A molecular-genetic analysis of polymorphism grapevine cultivars from collection NSC Tairov institute of viticulture and wine-making using 9 microsatellite loci was conducted. The allele size of SSR-loci was obtained (table 1). Near the allele size is shown comparison the results of SSR-analysis was got in laboratory by Tairov Institute of viticulture and winemaking. Table 1 DNA profiles of Ukrainian cultivars analysed by 9 microsatellite loci VVS VVMD5 VVMD7 VVMD7 No. Sample Allele1 Allele Comp. Allele1 Allele Allele1 Allele Comp. Allele1 Allele Comp. 1 Aligote 101 clone Cabernet Franc : : :185 3 Sukcholimansky beliy Smena Tairjan Cabernet Sauvignon clone : :185 7 Zagrey Jantar Aromatniy Odessky cherniy Muskat odesskiy
4 VRZAG6 VRZAG79 VVDM5 VVMD8 VVMD3 No. Allele1 Allele Comp. Allele1 Allele Comp. Allele1 Allele Allele1 Allele Allele1 Allele : : : : The previous results was obtained by silver staining and shows difference by near 3-4 bp. Statistical assay by allele frequency, quantity of homo- and heterozygous, and homozygosity was obtained. The statistical analysis of studied SSR-loci showed the high level of polymorphism (Table ). Table Indicators of informativeness DNA-marker system studied grape varieties
5 VVS 14 0,045 0, ,7 0, ,18 0, ,09 0, ,09 0, ,136 0, , ,045 0, ,045 0, ,136 0, ,996 0,1439 VVMD5 4 0,136 0, ,136 0, ,136 0, ,73 0, ,09 0, , ,18 0, ,045 0,0005 0,998 0,17366 VVMD7 37 0,409 0, ,045 0, ,045 0, ,18 0, ,7 0, , ,045 0, ,045 0,0005 0,998 0,60034 VVMD ,09 0, ,136 0, ,136 0, ,045 0, ,136 0, , ,364 0, ,09 0,0081 0,997 0,0609 Table
6 VRZAG6 18 0,05 0, ,05 0, ,3 0, , 0, , , 0,04 0 0, 0,04 1 0,15 VRZAG ,045 0, ,136 0, ,09 0, ,7 0, ,045 0, ,045 0, ,09 0, , ,09 0, ,09 0, ,09 0, ,045 0,0005 0,993 0,11865 VVDM5 39 0,5 0, ,15 0, ,5 0, , ,35 0,15 1 0,7 VVMD8 6 0,045 0, ,09 0, ,73 0, ,7 0, ,7 0, , ,045 0, ,045 0, ,045 0,0005 0,997 0, VVMD3 34 0,09 0, ,045 0, ,54 0, ,136 0, , ,09 0, ,09 0,0081 0,991 0,33641 Common results - - 7,11 1,67 8 0,79
7 The average allele number of the locus was 7,11. Average heterozygosity or polymorphism level (Не) of nine studied loci of analyzed samples was The next step of analysis was transforming the data from allele size into the n-code system (table 3). Transformed data of SSR-analysis to n-code Table 3 Sample VS VVMD 5 VVMD 7 VVMD5 VVMD8 VVMD3 VVMD 7 VRZag6 VrZag79 Aligote 101 clone N+10 N+14 N+4 N+16 N+6 N N+10 N+18 N+ N+ N+1 N+16 N+18 N N+ Cabernet Franc N+16 N+4 N+ N+16 N+6 N+30 N N+16 N+10 N+18 N+ N+0 N+4 N+1 N+16 N+6 N+4 N+16 Sukcholimansky beliy N+10 N+8 N+4 N+8 N+6 N+4 N+ N+10 N+16 N+18 N+ N+18 N- N+1 N+10 N+16 N N+4 Smena N+1 N+0 N+1 N+14 N+8 N+16 N+16 N+16 N+6 N+18 N+0 N+4 N+1 N+6 N+10 N+16 N+18 Tairian N+1 N+1 N+16 N+6 N+16 N N+10 N+16 N+40 N+ N+ N+8 N+10 N+16 N-4 N+8 Cabernet Sauvignon N+16 N+8 N+8 N+16 N+6 N+16 N+16 N+18 N+ N- N+1 N+10 N+16 N+4 Zagrey N+ N+14 N+4 N+1 N+6 N+14 N N+10 N+8 N+6 N- N+ N+10 N+16 N+6 N N+1 Jantar N+1 N+6 N+ N+14 N+14 N+16 N+10 N+16 N+6 N+ N+18 N+8 N+16 N+8 N+18 N+1 N+14 Aromatniy N+10 N+0 N+ N+8 N+14 N+16 N+ N+16 N+16 N+30 N+ N+36 N+4 N+1 N+6 N N+18 Odessky cherniy N+10 N+8 N+1 N+ N+6 N+0 N+ N+10 N+18 N+6 N+ N+1 N+16 N+10 N+4 N+14 Muskat odesskiy N+10 N+0 N+1 N+14 N+16 N+16 N+50 N N+36 N+8 N+1 N+10 N+0 (Fig. 1). The data was coded and analysed using MEGA 4 program for building genetic tree Fig. 1. Distribution tree studied grape varieties by genetic distances, calculated according to the SSR-analysis using UPGMA method (figures near the bifurcation branches - the authenticity of the selection of the cluster)
8 To improve the reliability of the results of cluster analysis bootstrap-test was conducted (Fig. ). Bootstrap method is loaded into the program MEGA 4, is most often used to test probability of formation branches on phylogenetic trees. Fig.. Tree obtained according bootstrap-test As shown in the tree, studied varieties are divided into two clusters. The first cluster is formed mainly technical cultivars, except table cultivar Tairian. And the second cluster is formed mainly table cultivars, except technical cultivars Muskat odesskiy and Aromatniy. The first cluster is formed by cultivars with mainly close genoms. The variety Sukcholimansky beliy is the result of a cross between the Chardonnay and Plavay (a Moldovan variety of white grape). Variety Aligote was obtained from a cross of Pino Noir and Gouais Blanc. Even the variety Chardonnay derived from a cross between the Pinot and Gouais Blanc (Heunisch) too. So the genetic distance between Sukcholimansky beliy and Aligote 101 is low (0,1801). The variety Odessky cherniy (Synonyms - Alibernet) - crossing of Alicante Bouschet x Cabernet Sauvignon was bred in 1950 in the NSC Tairov institute of viticulture and winemaking in Odessa. It is planted in Slovakia, Hungary and the Czech Republic. That's way genetic distance between variety Odessky cherniy and Cabernet Sauvignon is very low (0,1313). The cultivar Cabernet Sauvignon was a result of cross between Cabernet Franc x
9 Sauvignon blanc, and in this case Cabernet Sauvignon is close to Cabernet Franc on the tree (genetic distance 0,1801). Polymorphism of chloroplasts DNA As a result of chloroplast DNA analysis using ccmp10 chloroplast-marker polymorphism of grapevine varieties was derived (Fig. 3). Fig. 3. The results of electrophoresis by chloroplast DNA-marker ccmp 10 (M size standard marker 50 bp Ledder, Promega, 1 - Aligote 101 clone, - Cabernet Franc, 3 Sukcholimansky beliy, 4 - Smena, 5 - Tairian, 6 - Cabernet Sauvignon clone, 7 Zagrey, 8 - Jantar, 9 - Aromatniy, 10 - Odessky cherniy, 11 - Muskat odesskiy) Three polymorphic chlorotypes were observed: 1) 113 bp sample 1 (Aligote 101 clone); ) 114 bp 3, 5, 6, 7, 8, 9, 10 (Sukcholimansky beliy, Tairian, Cabernet Sauvignon clone, Zagrey, Jantar, Aromatniy, Odessky cherniy); 3) 115 bp, 4, 11 (Cabernet Franc, Smena, Muskat odesskiy). Interesting that the clone Cabernet Sauvignon doesn't have similar chlorotyp with parent cultivar Cabernet Franc. Because Cabernet Sauvignon inherited chloroplasts DNA from the mother's cultivar Sauvignon blanc. IV. Future collaboration with host institution (if applicable): The cultivars exchange between collections; Introduction of new clones and researches of resistance; Training of young scientist;
10 Publications; Development of genotyping and identification of cultivars using SSR-analysis; Somatic chimerism, genetic inheritance, and mapping of mutation in grapevine; Genetic diversity and geographical dispersal of Austrian and Ukraine cultivars using by microsatellite markers; V. Foreseen publications/articles resulting or to result from the STSM (if applicable): It is foreseen that after deepest data analysis publication of results from STSM will be performed in the Mitteilungen Klosterneuburg. VI. Confirmation by the host institution of the successful execution of the STSM: Valery Bocharova used her stay in our laboratories to get familiar with several methods for genotyping grapevine material. As she have done all steps of the protocols she is ready to reestablish her procedures at the lab at home institute. For several of her probes the complete way from isolation to the analysing of results was done. On the other side she had also the opportunity to genotype with chloroplast markers and cloning fragments for sequencing. During her stay she could give an evidence of her perfect manual and intellectual capacities as a researcher. Valery Bocharova is a hard working scientist with high demands for precision and quality for her research. On the other side she is skilled with high social and emotional competence that each chief would appreciate to have her in his scientific team. We wish her all the best for future scientific career.
MICROSATELLITE CHARACTERISTICS OF GRAPEVINE CULTIVARS INCLUDED TO UKRAINIAN STATE REGISTER OF PLANT VARIETIES.
MICROSATELLITE CHARACTERISTICS OF GRAPEVINE CULTIVARS INCLUDED TO UKRAINIAN STATE REGISTER OF PLANT VARIETIES. O. Karastan, N. Mulukina, O. Papina, G. Plachinda, l. Gerus, I. Kovalyova National Scientific
More informationWhere in the Genome is the Flax b1 Locus?
Where in the Genome is the Flax b1 Locus? Kayla Lindenback 1 and Helen Booker 2 1,2 Plant Sciences Department, University of Saskatchewan, Saskatoon, SK S7N 5A8 2 Crop Development Center, University of
More informationReasons for the study
Systematic study Wittall J.B. et al. (2010): Finding a (pine) needle in a haystack: chloroplast genome sequence divergence in rare and widespread pines. Molecular Ecology 19, 100-114. Reasons for the study
More informationLUISA MAYENS VÁSQUEZ RAMÍREZ. Adress: Cl 37 # 28-15, Manizales, Caldas, Colombia. Cell Phone Number:
LUISA MAYENS VÁSQUEZ RAMÍREZ Adress: Cl 37 # 28-15, Manizales, Caldas, Colombia. Cell Phone Number: 3013978734 E-mail: luisamayens@gmail.com PROFILE Agronomical engineer, Universidad de Caldas, Colombia.
More informationMapping and Detection of Downy Mildew and Botrytis bunch rot Resistance Loci in Norton-based Population
Mapping and Detection of Downy Mildew and Botrytis bunch rot Resistance Loci in Norton-based Population Chin-Feng Hwang, Ph.D. State Fruit Experiment Station Darr College of Agriculture Vitis aestivalis-derived
More informationTitle: Development of Simple Sequence Repeat DNA markers for Muscadine Grape Cultivar Identification.
Title: Development of Simple Sequence Repeat DNA markers for Muscadine Grape Cultivar Identification. Progress Report Grant Code: SRSFC Project # 2018 R-06 Research Proposal Name, Mailing and Email Address
More informationMolecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia)
Molecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia) T. Kuchta1, D. Pangallo2, Z. Godálová1, A. Puškárová2, M. Bučková2, K. Ženišová1, L. Kraková2
More informationCatalogue of published works on. Maize Lethal Necrosis (MLN) Disease
Catalogue of published works on Maize Lethal Necrosis (MLN) Disease Mentions of Maize Lethal Necrosis (MLN) Disease - Reports and Journals Current and future potential distribution of maize chlorotic mottle
More informationThe Identity and Parentage of the Variety Known in California as Petite Sirah
The Identity and Parentage of the Variety Known in California as Petite Sirah CAROLE P. MEREDITH 1., JOHN E. BOWERS, SUMMAIRA RIAZ, VANESSA HANDLEY, ELIZABETH B. BANDMAN, and GERALD S. DANGL DNA marker
More informationFruit and berry breeding and breedingrelated. research at SLU Hilde Nybom
Fruit and berry breeding and breedingrelated research at SLU 2014-11-11 Hilde Nybom Plant breeding: cultivar development Relevant breeding-related research Fruit and berry breeding at Balsgård Apple (Malus
More informationIdentification and Classification of Pink Menoreh Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers
RESEARCH Identification and Classification of Pink Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers Nandariyah a,b * adepartment of Agronomy, Faculty of Agriculture, Sebelas Maret
More informationWP Board 1054/08 Rev. 1
WP Board 1054/08 Rev. 1 9 September 2009 Original: English E Executive Board/ International Coffee Council 22 25 September 2009 London, England Sequencing the genome for enhanced characterization, utilization,
More informationCatalogue of vines grown in France Chardonnay B
Catalogue of vines grown in France http://plantgrape.plantnetproject.org UMT Géno-Vigne INRA - IFV - Montpellier SupAgro Edited on 11/04/2018 Chardonnay B Name of in France Chardonnay Origin This variety
More informationConfectionary sunflower A new breeding program. Sun Yue (Jenny)
Confectionary sunflower A new breeding program Sun Yue (Jenny) Sunflower in Australia Oilseed: vegetable oil, margarine Canola, cotton seeds account for >90% of oilseed production Sunflower less competitive
More informationAVOCADO GENETICS AND BREEDING PRESENT AND FUTURE
AVOCADO GENETICS AND BREEDING PRESENT AND FUTURE U. Lavi, D. Sa'ada,, I. Regev and E. Lahav ARO- Volcani Center P. O. B. 6, Bet - Dagan 50250, Israel Presented at World Avocado Congress V Malaga, Spain
More informationConstruction of a Wine Yeast Genome Deletion Library (WYGDL)
Construction of a Wine Yeast Genome Deletion Library (WYGDL) Tina Tran, Angus Forgan, Eveline Bartowsky and Anthony Borneman Australian Wine Industry AWRI Established 26 th April 1955 Location Adelaide,
More informationIntroduction to the use of molecular genotyping techniques
Introduction to the use of molecular genotyping techniques Gregorio López-Ortega, Almudena Bayo-Canha, Emma Skipper and Felicidad Fernández Budapest 3 rd -5 th of March STSM (Spain to UK) Pomological characterization
More informationChapter V SUMMARY AND CONCLUSION
Chapter V SUMMARY AND CONCLUSION Coffea is economically the most important genus of the family Rubiaceae, producing the coffee of commerce. Coffee of commerce is obtained mainly from Coffea arabica and
More informationCatalogue of vines grown in France Cabernet franc N
Catalogue of vines grown in France http://plantgrape.plantnetproject.org UMT Géno-Vigne INRA - IFV - Montpellier SupAgro Edited on 12/05/2018 Cabernet franc N Name of in France Cabernet franc Origin Cabernet
More informationGlobal Perspectives Grant Program
UW College of Agriculture and Natural Resources Global Perspectives Grant Program Project Report Instructions 1. COVER PAGE Award Period (e.g. Spring 2012): Summer 2015 Principle Investigator(s)_Sadanand
More informationAcademic Year 2014/2015 Assessment Report. Bachelor of Science in Viticulture, Department of Viticulture and Enology
Academic Year 2014/2015 Assessment Report Bachelor of Science in Viticulture, Department of Viticulture and Enology Due to changes in faculty assignments, there was no SOAP coordinator for the Department
More informationJCAST. Department of Viticulture and Enology, B.S. in Viticulture
JCAST Department of Viticulture and Enology, B.S. in Viticulture Student Outcomes Assessment Plan (SOAP) I. Mission Statement The mission of the Department of Viticulture and Enology at California State
More informationIdentification of haplotypes controlling seedless by genome resequencing of grape
Identification of haplotypes controlling seedless by genome resequencing of grape Soon-Chun Jeong scjeong@kribb.re.kr Korea Research Institute of Bioscience and Biotechnology Why seedless grape research
More informationNatural history of Trichinella britovi in the neighboring Mediterranean islands of Corsica and Sardinia
Workshop of National Reference Laboratories for Parasites Istituto Superiore di Sanità, Rome, Italy, 24-25 May, 2018 Natural history of Trichinella britovi in the neighboring Mediterranean islands of Corsica
More informationGenetic diversity of wild Coffee (Coffea arabica) and its implication for conservation
Genetic diversity of wild Coffee (Coffea arabica) and its implication for conservation Kassahun Tesfaye, Feyera Senbeta, Tamiru Oljira, Solomon Balemi, Govers, K., Endashaw Bekele, Borsch, T. Biodiversity
More informationSupplemental Data. Jeong et al. (2012). Plant Cell /tpc
Suppmemental Figure 1. Alignment of amino acid sequences of Glycine max JAG1 and its homeolog JAG2, At-JAG and NUBBIN from Arabidopsis thaliana, LYRATE from Solanum lycopersicum, and Zm- JAG from Zea mays.
More informationUse of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1
Agronomy Research 4(Special issue), 335 339, 2006 Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1 R. Rugienius*,
More informationTitle: Genetic Variation of Crabapples ( Malus spp.) found on Governors Island and NYC Area
Title: Genetic Variation of Crabapples ( Malus spp.) found on Governors Island and NYC Area Team Members: Jianri Chen, Zinan Ma, Iulius Sergiu Moldovan and Xuanzhi Zhao Sponsoring Teacher: Alfred Lwin
More informationZAIKA I.V. 1, SOZINOV A.A. 2, 3, KARELOV A.V. 2, KOZUB N.A. 2, FILENKO A.L. 4, SOZINOV I.A. 2 1
11. McNeil M.D., Kota R., Paux E., Dunn D., McLean R., Feuillet C., Li D., Kong X., Lagudah E., Zhang J.C., Jia J.Z., Spielmeyer W., Bellgard M., Apples R. BAC-derived markers for assaying the stem rust
More informationGenetic profiling of nine grapevine cultivars from Romania, based on SSR markers
Romanian Biotechnological Letters Vol. 15, No.1, Supplement, 10 Copyright 10 University of Bucharest Printed in Romania. All rights reserved ORIGINAL PAPER Genetic profiling of nine grapevine cultivars
More informationField identification, collection and evaluation of grapevine autochthonous cultivars
ERA 91/01 Preservation and establishment of true-to-type and virus free material of endangered grapevine cultivars in Croatia and Montenegro Field identification, collection and evaluation of grapevine
More informationSeparation of Ovotransferrin and Ovomucoid from Chicken Egg White
Animal Industry Report AS 662 ASL R3105 2016 Separation of and from Chicken Egg White Sandun Abeyrathne Iowa State University Hyunyong Lee Iowa State University, hdragon@iastate.edu Dong U. Ahn Iowa State
More informationAmpelographic description, berry oenological traits, and molecular characterisation of grapevine varieties grown in northern Greece
Ampelographic description, berry oenological traits, and molecular characterisation of grapevine varieties grown in northern Greece Merkouropoulos G 1, Ganopoulos I 1, Argiriou A 1, Zioziou E. 2, Koundouras
More informationPHYLOGENETICS ANALYSIS OF NORTH AMERICAN NATIVE CYNTHIANA/NORTON GRAPE VARIETY USING DNA MICROSATELLITE MARKERS
Proc. Fla. State Hort. Soc. 119:61-65. 2006. PHYLOGENETICS ANALYSIS OF NORTH AMERICAN NATIVE CYNTHIANA/NORTON GRAPE VARIETY USING DNA MICROSATELLITE MARKERS LELAN PARKER, PATRICIA BORDALLO AND VIOLETA
More informationCharacterization of Iranian grapevine cultivars using microsatellite markers
Vitis 42 (4), 185 192 (2003) Characterization of Iranian grapevine cultivars using microsatellite markers R. FATAHI 1), A. EBADI 1), N. BASSIL 2, 3), S. A. MEHLENBACHER 2) and Z. ZAMANI 1 1) Department
More informationTHE MANIFOLD EFFECTS OF GENES AFFECTING FRUIT SIZE AND VEGETATIVE GROWTH IN THE RASPBERRY
THE MANIFOLD EFFECTS OF GENES AFFECTING FRUIT SIZE AND VEGETATIVE GROWTH IN THE RASPBERRY II. GENE I2 BY D. L. JENNINGS Scottish Horticultural Research Institute, Dundee {Received 16 September 1965)...
More informationClass time required: Three forty minute class periods (an additional class period if Parts 6 and 7 are done).
Taste Blind? Core Concepts Receptors, nerve cell pathways, and taste areas of the brain are involved in sensing tastes. People differ in their response to taste sensations. A correlation is a relationship
More informationArmenian Alcoholic Beverages Market and Industry Overview
Avenue Consulting Group Strategy Operations Legal and Tax Armenian Alcoholic Beverages Market and Industry Overview Yerevan, 2015 Content Why This Report is Prepared 3 Armenian Alcoholic Beverages Market
More informationGenetic Diversity, Structure and Differentiation in Cultivated Walnut (Juglans regia L.)
Genetic Diversity, Structure and Differentiation in Cultivated Walnut (Juglans regia L.) M. Aradhya 1, K. Woeste 2 and D. Velasco 1 1 National Clonal Germplasm Repository, USDA-ARS, University of California,
More informationRESOLUTION OIV-OENO 576A-2017
RESOLUTION OIV-OENO 576A-2017 MONOGRAPH OF SACCHAROMYCES YEASTS THE GENERAL ASSEMBLY, In view of article 2, paragraph 2 iv of the Agreement of 3 April 2001 establishing the International Organisation of
More informationA Review of the Authentication of Wine Origin by Molecular Markers
A Review of the Authentication of Wine Origin by Molecular Markers Burçak İşçi 1,3, Hatice Kalkan Yildirim 2 and Ahmet Altindişli 1 ABSTRACT J. Inst. Brew. 115(3), 259 264, 2009 Viniculture is one of the
More informationInvestigations into the Origin of Norton Grape using SSR Markers
REFEREED MANUSCRIPT Proc. Fla. State Hort. Soc. 122:19 24. 2009. Investigations into the Origin of Norton Grape using SSR Markers ED STOVER 1*, MALLI ARADHYA 2, JUDY YANG 3, JOHN BAUTISTA 2, AND GERALD
More informationGenetic Relationships Among Pinots and Related Cultivars
Genetic Relationships Among Pinots and Related Cultivars FERDINAND REGNER 1., ALEXANDRA STADLBAUER 2, CORNELIA EISENHELD 3, and HERWlG KASERER 4 Genetic analysis based on more than 30 SSR (= "simple sequence
More informationSequential Separation of Lysozyme, Ovomucin, Ovotransferrin and Ovalbumin from Egg White
AS 662 ASL R3104 2016 Sequential Separation of Lysozyme, Ovomucin, Ovotransferrin and Ovalbumin from Egg White Sandun Abeyrathne Iowa State University Hyunyong Lee Iowa State University, hdragon@iastate.edu
More informationCOST action FA 0807 Scientific Report of Short-Term Scientific Missions (STSM) STSM Topic: STSM grantee: Host: Period: Place: Reference code:
COST action FA 0807 Integrated Management of Phytoplasma Epidemics in Different Crop Systems Scientific Report of Short-Term Scientific Missions (STSM) STSM Topic: Diagnostics of grapevine phytoplasma
More informationMolecular Systematics & Ethnobotany Case Study: Breadfruit
Molecular Systematics & Ethnobotany Case Study: Breadfruit Thanks to Tim Motley & Nyree Zerega for pictures and information. Hawaii, California, Bering Straight Bounty-hunting Pandora s Box Breadfruit
More informationGENETICS AND EVOLUTION OF CORN. This activity previews basic concepts of inheritance and how species change over time.
GENETICS AND EVOLUTION OF CORN This activity previews basic concepts of inheritance and how species change over time. Objectives for Exam #1: 1. Describe and complete a monohybrid ( one trait ) cross of
More informationCombining high throughput genotyping and phenotyping for the genetic improvement of table grapes in Chile
Combining high throughput genotyping and phenotyping for the genetic improvement of table grapes in Chile Pablo Cid, Inti Pedroso, Miguel García, Omar Essaú, Tim Kok and Paola Barba Instituto de investigaciones
More informationControlling Pierce s Disease with Molecular and Classical Breeding
Controlling Pierce s Disease with Molecular and Classical Breeding M. Andrew Walker Professor Louise Rossi Endowed Chair in Viticulture University of California, Davis Funding from CDFA PD/GWSS Board and
More informationGenetic relationships between Sardinian and Spanish viticulture: the case of Cannonau and Garnacha
Journal of Horticultural Science & Biotechnology (2009) 84 (1) 65 71 Genetic relationships between Sardinian and Spanish viticulture: the case of Cannonau and Garnacha By F. DE MATTIA 1, G. LOVICU 2, J.
More informationGenetic Similarities among Wine Grape Cultivars Revealed by Restriction Fragment-length Polymorphism (RFLP) Analysis
J. AMER. SOC. HORT. SCI. 121(4):620 624. 1996. Genetic Similarities among Wine Grape Cultivars Revealed by Restriction Fragment-length Polymorphism (RFLP) Analysis John E. Bowers and Carole P. Meredith
More informationFINAL REPORT TO AUSTRALIAN GRAPE AND WINE AUTHORITY. Project Number: AGT1524. Principal Investigator: Ana Hranilovic
Collaboration with Bordeaux researchers to explore genotypic and phenotypic diversity of Lachancea thermotolerans - a promising non- Saccharomyces for winemaking FINAL REPORT TO AUSTRALIAN GRAPE AND WINE
More informationNEPAL FISH BIODIVERSITY PROJECT. Update Report
NEPAL FISH BIODIVERSITY PROJECT Update Report May 29, 2016 1 Table of contents Sections Page No. 1. Overview 4 2. Site Characterization 5 3. Fish Sampling Training and Workshop 5 4. Sample Collection Technique
More informationMuseum Victoria CRC National Plant Biosecurity
1. PaDIL Species Factsheet Scientific Name: Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996 race 2 (Bacteria: Proteobacteria: Burkholderiales: Burkholderiaceae) Common Name Moko disease of banana
More informationOpening Remarks by Hon. Amelia Kyambadde Minister of Trade, Industry and Cooperatives
Opening Remarks by Hon. Amelia Kyambadde Minister of Trade, Industry and Cooperatives I am privileged to have been invited to officiate at the opening of this workshop. Let me use this opportunity to thank
More informationFood Allergen and Adulteration Test Kits
Food Allergen and Adulteration Test Kits Overview Neogen offers food allergen test kits to detect almond, egg, gliadin, hazelnut, milk, mustard, peanut, sesame, shellfish, soy and walnut residues (see
More informationMolecular Systematics & Ethnobotany Case Study: Breadfruit
Molecular Systematics & Ethnobotany Case Study: Breadfruit Thanks to Tim Motley & Nyree Zerega for pictures and information. Hawaii, California, Bering Straight Bounty-hunting Pandora s Box Breadfruit
More informationVITICULTURE AND ENOLOGY
VITICULTURE AND ENOLOGY Class L-25: Agricultural and Forest Science and Technology http://www.enol.unimi.it/ DIRECTOR OF THE BACHELOR S PROGRAMME Prof. Attilio Scienza Department of Crop Production Tree
More informationCERTIFICATE PROGRAMS VITICULTURE AND WINERY TECHNOLOGY VWT 130 General Viticulture VWT 172 Laboratory Analysis
Napa Valley College 2277 Napa-Vallejo Highway, Napa, CA. 94558 VITICULTURE AND WINERY TECHNOLOGY Dr. Stephen J. Krebs, Program Coordinator, (707) 253-3259, skrebs@napavalley.edu Bryan Avila, Winery Technology
More informationShazia Mannan COMSATS Institute of Information Technology Sahiwal Campus, Pakistan
Shazia Mannan COMSATS Institute of Information Technology Sahiwal Campus, Pakistan Citrus is one of the major export commodities of Pakistan and is grown in an area of 160,000 ha. Annual production of
More informationMBA 503 Final Project Guidelines and Rubric
MBA 503 Final Project Guidelines and Rubric Overview There are two summative assessments for this course. For your first assessment, you will be objectively assessed by your completion of a series of MyAccountingLab
More informationPhD THESIS. Rise of the effectiveness of the grape breeding with the investigation of the genetic background of the species. Gizella Györffyné Jahnke
PhD THESIS Rise of the effectiveness of the grape breeding with the investigation of the genetic background of the species Gizella Györffyné Jahnke Budapest, 2006 PhD School Name: Doctoral School of Interdisciplinary
More informationRESOLUTION OIV-ECO
RESOLUTION OIV-ECO 563-2016 TRAINING PROGRAMS FOR OENOLOGISTS THE GENERAL ASSEMBLY, based on the work of the FORMAT Expert Group, CONSIDERING the resolution OIV-ECO 492-2013 providing the definition of
More informationVirus Status of the Texas Grape Industry
Virus Status of the Texas Grape Industry 2017 Advanced GRAPE GROWER Workshop Hill Country University Center, Fredericksburg June 19-20 2017 Sheila McBride Program Extension Specialist Texas Plant Disease
More informationComplementation of sweet corn mutants: a method for grouping sweet corn genotypes
c Indian Academy of Sciences RESEARCH NOTE Complementation of sweet corn mutants: a method for grouping sweet corn genotypes S. K. JHA 1,2,N.K.SINGH 1,3 and P. K. AGRAWAL 1,4 1 Vivekananda Parvatiya Krishi
More informationCalvin Lietzow and James Nienhuis Department of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706
Precocious Yellow Rind Color in Cucurbita moschata Calvin Lietzow and James Nienhuis Department of Horticulture, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706 Amber DeLong and Linda Wessel-Beaver
More informationWe are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 3,350 108,000 1.7 M Open access books available International authors and editors Downloads Our
More informationProgress on the transferring Sclerotinia resistance genes from wild perennial Helianthus species into cultivated sunflower.
Progress on the transferring Sclerotinia resistance genes from wild perennial Helianthus species into cultivated sunflower Zhao Liu 1, Fang Wei 1, Xiwen Cai 1, Gerald J. Seiler 2, Thomas J. Gulya 2, Khalid
More informationCOST STSM Report. Action FP1203
COST STSM Report Action FP1203 STSM Applicant: Rogério Filipe Agostinho Louro, Institute of Mediterranean Agricultural and Environmental Sciences Universidade de Évora, Évora, PORTUGAL. Period: From 2014-03-23
More informationANNEX IX TO THE DECISION OECD SCHEME FOR THE VARIETAL CERTIFICATION OF SUGAR BEET AND FODDER BEET SEED
ANNEX IX TO THE DECISION FOR THE VARIETAL CERTIFICATION OF SUGAR BEET AND FODDER BEET SEED 109 ANNEX IX TO THE DECISION OECD SCHEME FOR THE VARIETAL CERTIFICATION OF SUGAR BEET AND FODDER BEET SEED 110
More information(Definition modified from APSnet)
Development of a New Clubroot Differential Set S.E. Strelkov, T. Cao, V.P. Manolii and S.F. Hwang Clubroot Summit Edmonton, March 7, 2012 Background Multiple strains of P. brassicae are known to exist
More informationGenotyping wine and table grape cultivars from Apulia (Southern Italy) using microsatellite markers
Vitis 41 (4), 183 187 (2002) Genotyping wine and table grape cultivars from Apulia (Southern Italy) using microsatellite markers L. ZULII, M. RUSSO and E. PETERLUGER Dipartimento di Produzione Vegetale
More informationSCAE CDS:! Introduction to coffee! The start of your journey into the SCAE Coffee Diploma System, for anyone with an interest in coffee!
SCAE CDS: Introduction to coffee The start of your journey into the SCAE Coffee Diploma System, for anyone with an interest in coffee Cimbali and MUMAC Academy are please to be able to offer SCAE Barista
More informationDraft Concept Paper for Coconut Research and Development in the South Pacific
Draft Concept Paper for Coconut Research and Development in the South Pacific COGENT Steering Committee Meeting Bangkok Thailand, June 2002 Introduction The South Pacific Island nations are collectively
More informationCharacterization of Citrus Cultivars and Clones in Cyprus through Microsatellite and RAPD Analysis
Articles A&EB Characterization of Citrus Cultivars and Clones in Cyprus through Microsatellite and RAPD Analysis T. Hvarleva 1, T. Kapari-Isaia 2, L. Papayiannis 2, A. Atanassov 1, A. Hadjinicoli 2, A.
More informationOverview of 'Maraska' and 'Oblačinska' sour cherries
COST Action FA1104 Sustainable Production of High Quality Cherries for the European Market Novi Sad, 15.-17. september 2014. Overview of 'Maraska' and 'Oblačinska' sour cherries Vokurka A., Njavro M.,
More information3.5 Citrus Greening (Huanglongbing) Disease in India : Present Status and Diagnostic Efforts
Page 129 3.5 Citrus Greening (Huanglongbing) Disease in India : Present Status and Diagnostic Efforts Das A. K. National Research Centre for Citrus, Amravati Road, Nagpur 440010, India. Among all diseases
More informationReniform Resistance from Texas Day Neutral Lines
Reniform Resistance from Texas Salliana R. Stetina Research Plant Pathologist Crop Genetics and Production Research Unit Stoneville, MS Cultural and Genetic Methods to Manage Reniform Nematode in Cotton
More informationTrends in branch of viticulture of Ukraine and perspectives of its innovative development
UDC 634.8 2015 Y.M. Gadzalo, Academician of the NAAS, Doctor of Agricultural Sciences, National Academy of Agrarian Sciences of Ukraine V.V. Vlasov, Corresponding Member of the National Academy of Sciences
More informationBATURIN S.O., KUZNETSOVA
1...,.. - (Fragaria x ananassa Duch.) //. 2010.. 14, 1.. 165-171. 2...,.. - Fragaria x Potentilla ( Frel) // -. 2011.. 15, 4.. 800 807. 3... Fragaria x ananassa Duch..... 2012. 16. 4... -. :, 2000.. 28
More informationAssessment of Management Systems of Wineries in Armenia
International Wine Conference "Global Trends and Best Practices in the Wine World: Implications and Recommendations for Armenia" November 24, 2017 Assessment of Management Systems of Wineries in Armenia
More informationSommelier 9543 Certificate III in Hospitality (Operations) Sommeliers
Sommelier 9543 Certificate III in Hospitality (Operations) Sommeliers National Code: AQF THH33002 This course is for people who want to work in the hospitality industry and specialize in the role of Sommelier
More informationGenetic diversity of native Pinus sylvestris L. of Gerês accessed by SSR markers (MICROSAT PSYLV)
Genetic diversity of native Pinus sylvestris L. of Gerês accessed by SSR markers (MICROSAT PSYLV) UTAD, Vila Real Portugal BFW, Austria This work was partially funded by: FEDER funds through the Programa
More information2nd working meeting of the Regional Expert Advisory Working Group on Wine in South Estern Europe 14/12/ /12/2015, Skopje, Macedonia
2nd working meeting of the Regional Expert Advisory Working Group on Wine in South Estern Europe 14/12/2015 16/12/2015, Skopje, Macedonia EU legislation - Implementing Regulation COMMISSION REGULATION
More informationCatalogue of vines grown in France Gamay N
Catalogue of vines grown in France http://plantgrape.plantnetproject.org UMT Géno-Vigne INRA - IFV - Montpellier SupAgro Edited on 18/02/2018 Gamay N Name of in France Gamay Origin Based on published genetic
More informationWelcome to Balsgård. Department of Plant Breeding and Biotechnology, SLU.
Welcome to Balsgård Department of Plant Breeding and Biotechnology, SLU www.ltj.slu.se/balsgard People and money At Balsgård we have 15 20 employees (scientists, technicians, administrative personnel)
More informationCatalogue of vines grown in France Grenache N
Catalogue of vines grown in France http://plantgrape.plantnetproject.org UMT Géno-Vigne INRA - IFV - Montpellier SupAgro Edited on 14/05/2018 Grenache N Name of in France Grenache Origin This variety was
More informationItalian Register of Vine Varieties Registration of varieties and clones
Italian Register of Vine Varieties Registration of varieties and clones Paolo Giorgetti Ministero delle politiche agricole alimentari e forestali D. G. Sviluppo Rurale DISR V Servizio fitosanitario centrale,
More informationEUREKA project 5885! SQAH /Czech-English cooperation /
EUREKA project 5885! SQAH /Czech-English cooperation 2011-2014/ Development of sustainable quality aroma dwarf hops in both the CR and the U.K. to supply brewing worldwide Vyšlechtění jemných aromatických
More informationPROJECTS FUNDED BY THE SOUTHERN REGION SMALL FRUIT CONSORTIUM FOR 2011
PROJECTS FUNDED BY THE SOUTHERN REGION SMALL FRUIT CONSORTIUM FOR 2011 Title: Determination of Flower Type and Other Traits in Muscadine Grape Using Molecular Markers Final or Progress Report(Indicate
More informationINDIAN COUNCIL OF AGRICULTURAL RESEARCH DIRECTORATE OF RAPESEED-MUSTARD RESEARCH, BHARATPUR, INDIA
INDIAN COUNCIL OF AGRICULTURAL RESEARCH DIRECTORATE OF RAPESEED-MUSTARD RESEARCH, BHARATPUR, INDIA Pathogenic variability of Sclerotinia sclerotiorum isolates on Brassica differentials Pankaj Sharma ICAR-Directorate
More information4 Breeding Corn Fa r m e r s t h r o u g h o u t t h e world grow crops and raise animals to sustain
4 Breeding Corn Fa r m e r s t h r o u g h o u t t h e world grow cros and raise animals to sustain their families and communities, and to earn a living. Livestock and most cros reroduce by sexual reroduction
More informationThe Mediterranean Genetic Code - Grapevine and Olive, Edited by Danijela Poljuha
THE MEDITERRANEAN GENETIC CODE - GRAPEVINE AND OLIVE Edited by Danijela Poljuha and Barbara Sladonja The Mediterranean Genetic Code - Grapevine and Olive http://dx.doi.org/10.5772/3442 Edited by Danijela
More informationCatalogue of vines grown in France Pinot noir N
Catalogue of vines grown in France http://plantgrape.plantnetproject.org UMT Géno-Vigne INRA - IFV - Montpellier SupAgro Edited on 09/02/2018 Pinot noir N Name of vine variety in France Pinot noir Origin
More informationGenetic structure in cultivated grapevines is linked to geography and human selection.
Genetic structure in cultivated grapevines is linked to geography and human selection. Roberto Bacilieri, Thierry Lacombe, Loic Le Cunff, Manuel Di Vecchi-Staraz, Valerie Laucou, Blaise Genna, Jean-Pierre
More informationAssessment of the uniformity and stability of grapevine cultivars using a set of microsatellite markers
Assessment of the uniformity and stability of grapevine cultivars using a set of microsatellite markers M. Dolores Vélez 1 and Javier Ibáñez 2 Instituto Madrileño de Investigación y Desarrollo Rural, Agrario
More informationis pleased to introduce the 2017 Scholarship Recipients
is pleased to introduce the 2017 Scholarship Recipients Congratulations to Elizabeth Burzynski Katherine East Jaclyn Fiola Jerry Lin Sydney Morgan Maria Smith Jake Uretsky Elizabeth Burzynski Cornell University
More informationKiwifruit Breeding & Genomics
Kiwifruit Breeding & Genomics Luis Gea, Breeding & Genomics Genetics for productivity» 3-4 years to flowering» 10 years breeding to production cycle» Progeny test + RRS» Backcross / inbreeding /sex Genetics
More informationIdeas for group discussion / exercises - Section 3 Applying food hygiene principles to the coffee chain
Ideas for group discussion / exercises - Section 3 Applying food hygiene principles to the coffee chain Activity 4: National level planning Reviewing national codes of practice and the regulatory framework
More informationGenetic diversity in native Bulgarian grapevine germplasm (Vitis vinifera L.) based on nuclear and chloroplast microsatellite polymorphisms
Vitis 48 (3), 115 121 (2009) Genetic diversity in native Bulgarian grapevine germplasm (Vitis vinifera L.) based on nuclear and chloroplast microsatellite polymorphisms T. DZHAMBAZOVA 1), I. TSVETKOV 1),
More information