CERTIFICATE OF ANALYSIS - GC PROFILING
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1 Date : April 24, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18D17-HBN9-1-CC Customer identification : Peppermint Oil - India Type : Essential oil Source : Mentha x piperita Customer : Health & Beauty Natural Oils ANALYSIS Method: PC-PA J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Sylvain Mercier, M. Sc., Chimiste Analysis date : April 19, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/11
2 PHYSICOCHEMICAL DATA Physical aspect: Faintly yellow liquid Refractive index: ± (20 C) COMPLIANCE WITH NF T :2008 OIL OF PEPPERMINT (MENTHA X PIPERITA) Compound USA type Other type Min. % Max. % Min. % Max. % Observed % Complies? 3-Octanol Yes 1,8-Cineole Yes Limonene Yes Sabinene hydrate* No Menthone Yes Isomenthone Yes Menthofuran Yes Neomenthol Yes Menthol Other Pulegone Yes Menthyl acetate Yes β-caryophyllene Other Refractive index Yes *The AFNOR norms nomenclature for this compound appears to be confounding. The value indicated in the table is for cis-sabinene hydrate, as recommended for this compound by Robert P. Adams. CONCLUSION No adulterant, contaminant or diluent has been detected using this method. The oil marginally does not comply with the ISO standard for non-usa peppermint oil. Page 2/11
3 ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Isobutanol tr 1.00* Aliphatic alcohol Isovaleral Aliphatic aldehyde 2-Methylbutyral 0.01 tr Aliphatic aldehyde 2-Ethylfuran tr tr Furan Isoamyl alcohol Aliphatic alcohol 2-Methylbutanol Aliphatic alcohol (3Z)-Hexenol tr tr Aliphatic alcohol Hexanol tr Aliphatic alcohol trans-2,5-diethyltetrahydrofuran Furan Hashishene tr 0.69* Monoterpene α-thujene Monoterpene α-pinene 0.68 [0.69]* Monoterpene Camphene 0.06* 0.03 Monoterpene α-fenchene [0.06]* 0.01 Monoterpene 3-Methylcyclohexanone [0.06]* 0.04* Aliphatic ketone Thuja-2,4(10)-diene * Monoterpene Sabinene 1.30* [0.31]* Monoterpene β-pinene [1.30]* [1.00]* Monoterpene Octen-3-ol Aliphatic alcohol Octan-3-one * Aliphatic ketone Myrcene Monoterpene Octan-3-ol Aliphatic alcohol α-phellandrene 0.04* 0.02 Monoterpene Pseudolimonene [0.04]* 0.01 Monoterpene Δ3-Carene tr 0.01 Monoterpene α-terpinene Monoterpene para-cymene Monoterpene Limonene 7.28* 2.11 Monoterpene 1,8-Cineole [7.28]* 5.15 Monoterpenic ether (Z)-β-Ocimene Monoterpene (E)-β-Ocimene 0.03 [0.04]* Monoterpene γ-terpinene Monoterpene cis-sabinene hydrate 0.14* 2.11* Monoterpenic alcohol para-mentha-3,8-diene [0.14]* 0.01 Monoterpene cis-linalool oxide (fur.) Monoterpenic alcohol Octanol Aliphatic alcohol Terpinolene 0.08* 0.07 Monoterpene para-cymenene [0.08]* 0.01* Monoterpene trans-sabinene hydrate Monoterpenic alcohol 2-Methylbutyl isovalerate? Aliphatic ester Linalool * Monoterpenic alcohol 2-Methylbutyl 2-methylbutyrate Aliphatic ester Amyl isovalerate 0.03 [0.04]* Aliphatic ester β-thujone 0.01 [0.01]* Monoterpenic ketone cis-para-menth-2-en-1-ol 0.02 [0.14]* Monoterpenic alcohol allo-ocimene 0.01* 0.01 Monoterpene Octan-3-yl acetate [0.01]* tr Aliphatic ester Isopulegol 0.15* 5.46* Monoterpenic alcohol Page 3/11
4 neo-isopulegol [0.15]* [5.46]* Monoterpenic alcohol Menthone Monoterpenic ketone Isomenthone 5.21* 3.34* Monoterpenic ketone Menthofuran [5.21]* [2.11]* Monoterpenic ether neo-menthol Monoterpenic alcohol δ-terpineol Monoterpenic alcohol Terpinen-4-ol 46.73* 0.32* Monoterpenic alcohol Menthol [46.73]* Monoterpenic alcohol Isomenthol [46.73] [0.32]* Monoterpenic alcohol α-terpineol * Monoterpenic alcohol neoiso-menthol [0.61] 0.32 Monoterpenic alcohol trans-isopiperitenol Monoterpenic alcohol Unknown 0.02 Unknown trans-piperitol * Monoterpenic alcohol Unknown 0.04 Unknown Citronellol * Monoterpenic alcohol Pulegone Monoterpenic ketone Carvone Monoterpenic ketone Piperitone Monoterpenic ketone Decanol 0.38* [0.22]* Aliphatic alcohol neo-menthyl acetate [0.38]* 0.17 Monoterpenic ester 2-Ethylmenthone? 0.02 Aliphatic ketone Dihydroedulan I Terpenic ether Menthyl acetate 5.37 [5.46]* Monoterpenic ester Dihydroedulan II Terpenic ether Isomenthyl acetate Monoterpenic alcohol Bicycloelemene 0.11 [3.34]* Sesquiterpene Piperitenone Monoterpenic ketone α-cubebene Sesquiterpene Evodone Monoterpenic ketone α-copaene Sesquiterpene β-bourbonene Sesquiterpene 1,5-diepi-β-Bourbonene Sesquiterpene β-elemene * Sesquiterpene Unknown 0.03 Sesquiterpene Isocaryophyllene 0.02 [5.46]* Sesquiterpene β-caryophyllene 2.67 [2.56]* Sesquiterpene β-copaene Sesquiterpene trans-α-bergamotene 0.02 [2.56]* Sesquiterpene Isogermacrene D Sesquiterpene α-humulene 0.34 [45.92]* Sesquiterpene Muurola-4,11-diene Sesquiterpene 9-epi-β-Caryophyllene 0.16* [45.92]* Sesquiterpene (E)-β-Farnesene [0.16]* 0.12 Sesquiterpene Germacrene D 0.47 [0.77]* Sesquiterpene Menthalactone Monoterpenic lactone Viridiflorene 0.17* 0.12 Sesquiterpene Bicyclogermacrene [0.17]* 0.10 Sesquiterpene γ-cadinene 0.02 [0.02]* Sesquiterpene δ-cadinene Sesquiterpene (E)-Nerolidol Sesquiterpenic alcohol Spathulenol Sesquiterpenic alcohol Page 4/11
5 Caryophyllene oxide 0.06* 0.05 Sesquiterpenic ether Caryophyllene oxide isomer [0.06]* 0.01 Sesquiterpenic ether Viridiflorol Sesquiterpenic alcohol Total identified 98.90% 98.15% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 5/11
6 This page was intentionally left blank. The following pages present the complete data of the analysis. Page 6/11
7 Page 7/11
8 Page 8/11
9 FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Isobutanol tr 2.14* Isovaleral Methylbutyral tr 2-Ethylfuran tr tr Isoamyl alcohol Methylbutanol (3Z)-Hexenol tr tr Hexanol tr trans-2,5- Diethyltetrahydrofuran Hashishene tr 1.39* α-thujene α-pinene * 996 [0.69] Camphene 3.20* α-fenchene 3.20* 940 [0.06] Methylcyclohexanone 3.20* 940 [0.06] 4.78* Thuja-2,4(10)-diene * Sabinene 3.61* * 1089 [0.31] β-pinene 3.61* 969 [1.30] 2.14* 1071 [1.00] Octen-3-ol Octan-3-one * Myrcene Octan-3-ol α-phellandrene 4.06* Pseudolimonene 4.06* 999 [0.04] Δ3-Carene tr α-terpinene para-cymene Limonene 4.48* ,8-Cineole 4.48* 1026 [7.28] (Z)-β-Ocimene (E)-β-Ocimene * 1216 [0.04] γ-terpinene cis-sabinene hydrate 5.06* * para-mentha-3,8-diene 5.06* 1062 [0.14] cis-linalool oxide (fur.) Octanol Terpinolene 5.39* para-cymenene 5.39* 1083 [0.08] 6.40* trans-sabinene hydrate Methylbutyl isovalerate? Linalool * Methylbutyl 2- methylbutyrate Amyl isovalerate * 1265 [0.04] Page 9/11
10 (12)] trans-piperitol * Unknown [m/z 146, 145 (94), 43 (72), 99 (41), 81 (29), 115 (25), 86 (24)...] β-thujone * 1390 [0.01] cis-para-menth-2-en-1- ol * 1520 [0.14] allo-ocimene 6.08* Octan-3-yl acetate 6.08* 1127 [0.01] tr Isopulegol 6.28* * neo-isopulegol 6.28* 1140 [0.15] 8.20* 1524 [5.46] Menthone Isomenthone 6.57* * Menthofuran 6.57* 1159 [5.21] 6.98* 1432 [2.11] neo-menthol δ-terpineol Terpinen-4-ol 6.85* * Menthol 6.85* 1177 [46.73] Isomenthol [46.73] 8.65* 1559 [0.32] α-terpineol * neoiso-menthol [0.61] trans-isopiperitenol Unknown [m/z 43, 99 (84), 81 (46), 986 (43), 126 (36), 71 (28) Citronellol * Pulegone Carvone Piperitone Decanol 8.30* * 1732 [0.22] neo-menthyl acetate 8.30* 1274 [0.38] Ethylmenthone? Dihydroedulan I Menthyl acetate * 1524 [5.46] Dihydroedulan II Isomenthyl acetate Bicycloelemene * 1440 [3.34] Piperitenone α-cubebene Evodone α-copaene β-bourbonene ,5-diepi-β Bourbonene β-elemene * Unknown [m/z 106, 119 (99), 43 (78), 91 (74), 105 (60), 134 (55) (19)] Isocaryophyllene * 1524 [5.46] Page 10/11
11 β-caryophyllene * 1544 [2.56] β-copaene trans-α-bergamotene * 1544 [2.56] Isogermacrene D α-humulene * 1614 [45.92] Muurola-4,11-diene epi-β-Caryophyllene 10.86* * 1614 [45.92] (E)-β-Farnesene 10.86* 1456 [0.16] Germacrene D * 1652 [0.77] Menthalactone Viridiflorene 11.29* Bicyclogermacrene 11.29* 1488 [0.17] γ-cadinene * 1705 [0.02] δ-cadinene (E)-Nerolidol Spathulenol Caryophyllene oxide 12.36* Caryophyllene oxide isomer 12.36* 1572 [0.06] Viridiflorol Total identified 98.90% 98.15% Total reported 98.98% 98.15% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total : Peaks apexes were resolved, but peaks overlapped and were summed for analysis tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 11/11
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1 Sample: Client: Sample: 21 Drops Batch # 0614/1 CAS Number 8006-81-3 Type: Essential Oil Conclusion: No adulterants, diluents, or contaminants were detected via this method. X Validated By: Phone: 317-361-5044
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