BOOTLEG BREW SCIENCE. capture culture contribute NATIONAL HOMEBREW CONFERENCE 2014 GRAND RAPIDS, MI USA

Transcription

1 BOOTLEG BREW SCIENCE capture culture contribute NATIONAL HOMEBREW CONFERENCE 2014 GRAND RAPIDS, MI USA

2 ] IMBED VIDEO HERE 2

3 WHO AM I? Jeff Mello Chief Yeast Wrangler at Bootleg Biology LLC Homebrewer Yeast Geek Pseudo Scientist From Seattle > DC/Arlington > Nashville, TN Contact FB: facebook.com/ bootlegbiology 3

4 WHAT IS BOOTLEG BIOLOGY? Open- source yeast (and wild bugs) project Goal: Create the most diverse library of microbes for the creation of fermented foods and beverages All microbes captured only from bootleg sources Backyard s, kombucha, yogurt, honey, fruit, bottle dregs, and whatever else we can get our hands on Bootleg Biology is the first organization to pioneer the collection and cultivation of official yeast strains for specific postal codes as part of the Local Yeast Project, beginning with the original S. Arlingtonesis (22204) Enthusiasts across the globe contribute yeast or house cultures to the project, with the shared goal of enriching everyone s access to diverse & local microbes 4

5 WHY BE BOOTLEG? OUR ETHOS: INDEPENDENT We are brewers, we like doing things our way No one/corp can tell us what to like or how something should be made LOCAL Fresh Food belongs to a specific place and has a local character Made by our family, friends and neighbors: craft brewers, local farmers CREATIVE Food should be unique and have a personality Prepared by someone who cares RESPECT FOR THE PAST Preserving cultural legacy Honoring traditions EDUCATION & LEARNING 5

6 LOCAL YEAST PROJECT The French call it terroir, we call it the Local Yeast Project. If you want to make a beer truly local, you ve got to have a local yeast Every city and region across the globe is chock full of wild yeast ready and willing to make a lunch out of your barley sugar The characteristics of local yeast strains will impart a taste and aroma unlike any other, creating beer styles that are totally unique to the sourced region Bootleg Biology already has 40+ local strains with more in the pipeline: States: AZ, CO, KS, KY, LA, NC, TN, TX, VA, WA Countries: Bulgaria, Italy, Portugal, England, Ireland, Costa Rica, United Kingdom, (Antarctica?) 6

7 7

8 EXPERIMENTERS Perrin Brewing (MI) Modern Times (CA) Libertine Pub (CA) Highland Park Brewery (CA) Natty Greene s (NC) Forrest & Main (PA) 8

9 WHAT ARE WE DRINKING? PART 1 9

10 Saison du Backyard Demo beer for showing off the clean flavors possible with isolated wild yeast strains Simple grain bill: mostly pilsner malt, small amounts of wheat malt, caravienne and candi syrup Fermented solely with original Bootleg Biology backyard yeast strain, S. arlingtonesis 10

11 DIYeast: How to Wrangle Yeast tools of the trade capture yeast create agar plates streak plates isolate yeast 11

12 TOOLS OF THE TRADE 12

13 CAPTURE: THREE METHODS Wild Yeast Starter Jar: Tricky but romantic Fruit & Flora: Easier Sterile Cotton Swabs: Easiest 13

14 WILD YEAST STARTER JAR Day 1: Fill jar w/pre- boiled gravity, slightly- hopped, acidified wort Cover w/cheesecloth/lid and set outside overnight Day 2: hrs later, bring inside, cover w/foil or pour in growler w/air lock Leave in warm, dark place Next 2-4 weeks: Watch for/verify signs of fermentation Wort must fully ferment before proceeding Toss jars w/abnormal colored liquid/non- mold masses Refrigerate for a few days to force yeast to flocculate Sensory Evaluation: Scrape off any mold Smell good? OK! Use pipette to remove sample from below surface Taste?: It ll be bitter or sour 14

15 FRUIT & FLORA Hand- pick wild or backyard fruit/flora Don t use fruit already on ground Pick the ripest or decomposing fruit Flowers: look for pollen or nectar (like honeysuckle) If travelling, put in empty test tube At home, insert fruit/flora into test tube w/ gravity wort (ideally sterile) Shake it up a couple times a day! Rest in warm, dark place for roughly 2 weeks Yeast sediment will form at bottom 15

16 STERILE COTTON SWABS Rub sterile swab on any surface with wild yeast Large fruit, plants with dusting (that dust on grapes/plums is yeast!) Foedres/barrels, yeast rich brewing environments Place swab sample in empty test tube for storage Once back home, treat swab like fruit (e.g. dunk in wort, shake, rest) 16

17 - BOOTLEG INTERLUDE - Yeast & Bug Porn Quiz #1 17

18 WHICH OF THESE IS BRETTANOMYCES ON AGAR?

19 AND THE ANSWER IS ALL OF THEM YOU RE ALL WINNERS! 19

20 REMAINING STEPS MUST OCCUR IN DRAFT-FREE AREA 20

21 WHAT IS A DRAFT-FREE AREA? Any enclosed space with solid, non- porous surfaces: Ideal: Cabinets, Closets, Closed Rooms without open vents Better than nothing: Card board box on its side Don t forget to disinfect all surfaces: Ideal: Lysol (quats/alcohol), rubbing alcohol Better than nothing: Star San Bonus: Room with HEPA filtered air 21

22 CREATING AGAR PLATES Recipe: 300ml low- grav wort/5g agar/dash yeast nutrient Boil until evenly mixed Let pot cool for ~5 minutes, but not long enough for liquid to set Pour liquid evenly into petri dish bottoms Set in draft- free area with lids off until agar cools and has set (solid enough to stick to plate, gelatin- like) Once cooled, put lids back on and turn upside down Cut 1/2 inch length strips of ParaFilm Remove paper side, and stretch over edge where lid and agar- filled bottom meet Always keep agar plates upside down to prevent contamination Place upside- down dishes inside a re- sealable bag in the refrigerator until ready to use Some plates will mold! 22

23 STREAKING VIDEO 23

24 STREAK PLATES Prepare: Grab agar plate from fridge, remove ParaFilm Unbend paper clip Tip: Practice w/ pure brewer s yeast before trying with wild yeast cultures Sterilize end under flame until it gets red hot (hold with clothespin if you can t take the heat) Let cool for roughly a minute Streak: Dip sterilized end of paper clip into liquid containing yeast Flip agar plate over and remove lid Drag p.c. lightly from edge to edge of agar plate 3 times Sterilize paper clip, let cool Drag 3 lines again but crossing over end of previous lines; sterilize Repeat 2 more times until 4 sets of lines are drawn Store: Put lid back on plate. Turn upside down (agar side up) Incubate in dark area until large, distinct round, whiteish colonies appear (2 days, week, longer) 24

25 ISOLATING YEAST Snag a new plate from the fridge and remove ParaFilm Grab your previously streaked plate. It should be a cornucopia of microbes (including mold). This is ok! Sterilize the paper clip under a flame Look for medium- sized, white to off- white, nicely round, individual colonies Pick up a small amount from one colony w/paper clip Restreak new plate (continue restreaking plates until you have one where all the colonies growing have the same shape and size) Select one colony from uniform plate using sterile p.c., dunk into vial with 10ml of sterilized low- gravity wort (1.020 or less) Congrats! You now have the world s smallest yeast starter Build up to pitchable quantity (10ml to 100ml to 1L) and toss into a test batch of homebrew or bank for later use 25

26 26

27 YOU HAVE THE KNOWLEDGE. NOW WHAT? Shameless plug alert. 27

28 BACKYARD YEAST WRANGLING TOOL KIT Everything the experimental homebrewer needs to capture wild bugs, create agar plates, and isolate wild yeast! 28

29 INCLUDED IN THE KIT 29

30 CONTRIBUTE & BECOME A PART OF BOOTLEG BIOLOGY For each new viable local isolated strain provided to Bootleg Biology, you get: One- to- one exchange of any culture Bootleg Biology offers with free shipping If you have a wild culture and don t want to try isolating, send it to us, and if we isolate a viable strain we ll send one back to you Recognition as the Wrangler of your strain(s) on the Big Board, with a link to your preferred web presence (Blog, Instagram, Twitter, etc) One time 25% discount on Bootleg Biology merch and swag 2 of our homebrew Contributors have gone on to start their own breweries 30

31 - BOOTLEG INTERLUDE - Yeast & Bug Porn Quiz #2 31

32 WHICH IS LACTOBACILLUS UNDER MAGNIFICATION?

33 AND THE ANSWER IS #2! 33

34 WHAT ARE WE DRINKING? PART 2 34

35 Berliner Bear Take on a Berlinerweisse with added stout grain bill Pilsner/wheat malt base mashed, drained into carboy Soured with Lactobacillus isolated from my homemade kimchi for 48 hours at ~40C/105F Racked into kettle, dark grains steeped 10 minutes, wort boiled for 20 min w/ small amount of hops Fermented out with S. arlingtonesis 3.2 ph, 3% ABV 35

36 BOOTLEG BREW SCIENCE F A Q 36

37 WHAT IS THE DIFFERENCE BETWEEN STERILE & SANITARY? Sterile = Absence of microbial life, not exposed to the naked air. Commonly achieved through wet heat (autoclave/pressure cooker) or flame (bunsen burner/gas range). Boiling does not produce sterile conditions Sanitary = Acceptable number of microbes or inhibition of growth. Does not mean the absence of life. 37

38 HOW DO I STERILIZE AT HOME? Pressure cooker = bootleg brewer s best friend To greatly increase chances of isolating and maintaining pure cultures, anything that comes in contact with yeast culture should be sterilized (starter flask, stir bar, test tubes) 38

39 HOW DO I KNOW WHAT I VE ISOLATED? Microbe Portrait Gallery on bootlegbiology.com Pictures of Brewer s Yeast, Brettanomyces, Lactobacillus Growth on agar, under magnification (yeast 800X, bacteria 2000X) 39

40 HOW DO I KNOW I M WORKING WITH A PURE CULTURE? Microscopes are essential in knowing if your culture is pure That being said, just double- check your work: Streak plates, restreak plates and streak them again Keep streaking until you see uniform colony morphology on a plate Sterilize equipment and use aseptic technique 40

41 MORE YEAST WRANGLING INFO bootlegbiology.com/diy you saw that coming Eureka Brewing eurekabrewing.wordpress.com BKYeast bkyeast.wordpress.com Sui Generis suigenerisbrewing.blogspot.ca Brew Science 41

42 SOME THINGS IN LIFE REALLY ARE FREE First 100 people to like us on Facebook, Instagram, or Twitter and come by the GRiST booth (#27) at Club Night Free Training Loop & Agar Plate: Do your first test streaking on us before investing in kit or full 42

43 THANK YOU! Q & A Time. 43