5 ng/l 2.5 ng/l June 2002 July ng/l 1 ng/l August 2008 August Cheney MIB Zimmerman et al GC/MS^#

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1 Supplementary tables S1 & S2 and supplementary figures 1 & 2. Harris et al. (216). Combined effects of nitrogen to phosphorus and nitrate to ammonia ratios on cyanobacterial metabolite concentrations in eutrophic Midwestern Supplemental Table S1. Limit of detection (LOD), number (.) of samples below the LOD, and total number of samples in each data set for each reservoir.. Reservoir Analyte Analytical method LOD Half of LOD Date range below LOD. of samples Indy reservoirs* NO 3 EPA 3..2 mg/l.1 mg/l all Indy reservoirs* NH 3 EPA mg/l.1 mg/l all Cheney NO 3 EPA 3..1 mg/l.5 mg/l all Cheney NH 3 APHA 45- NH 3 D.3 mg/l.15 mg/l all All reservoirs Microcystin ELISA $.1 µg/l.5 µg/l all Eagle Creek Geosmin APHA 64D - GC/MS^ 2 ng/l 1 ng/l all 2 37 Geist and Morse Geosmin APHA 64D - GC/MS^ 3 ng/l 1.25 ng/l all 5 8 Eagle Creek MIB APHA 64D - GC/MS^ 2 ng/l 1 ng/l all 2 37 Geist & Morse MIB APHA 64D - GC/MS^ 3 ng/l 1.25 ng/l all 12 8 Cheney Geosmin APHA 64D - GC/MS^ 3 ng/l 1.5 ng/l May 21 May Cheney Geosmin Zimmerman et al GC/MS^# 5 ng/l 2.5 ng/l June 22 July Cheney Geosmin Zimmerman et al GC/MS^# 2 ng/l 1 ng/l August 28 August Cheney MIB Zimmerman et al GC/MS^# 2 ng/l 1 ng/l August 28 August Cheney Geosmin/MIB Zimmerman et al GC/MS^# 1 ng/l.5 ng/l September 212 December Cheney Geosmin/MIB Zimmerman et al GC/MS^# 1 ng/l.5 ng/l September 212 December Cheney MIB Zimmerman et al GC/MS^# 5 ng/l 2.5 ng/l May 21 July # Zimmerman et al. 22 uses similar GC/MS procedures as APHA 64D ^ GC/MS = gas chromatography/mass spectrometry * Indy reservoirs include Eagle Creek, Geist, and Morse reservoirs surrounding Indianapolis, IN $ ELISA = enzyme-linked immunosorbent assay Supplementary material to: Harris et al. (216). Inland Waters 6pp DOI: /IW

2 Supplementary tables S1 & S2 and supplementary figures 1 & 2. Harris et al. (216). Combined effects of nitrogen Supplemental Table S2. Cyanobacteria, and their capability to fix N 2, present in the study. Asterisks indicate species where the capability to fix N 2 is likely strain specific. Genus species N 2 -fixer? Genus species N 2 -fixer? Order: Chroococcales /39 Order: Oscillatoriales 8/12 Aphanocapsa delicatissima Lyngbya contorta Yes Aphanocapsa holsatica Lyngbya limnetica Yes Aphanocapsa incerta Lyngbya sp. Yes Aphanocapsa planctonica Oscillatoria anguina Yes* Aphanocapsa pulchra Oscillatoria limnetica Yes* Aphanocapsa sp. Oscillatoria nitida Yes* Aphanothece clathrata Oscillatoria sp. Yes* Aphanothece minutissima Oscillatoria tenuis Yes* Aphanothece sp. Phormidium sp. Aphanothece stagnina Planktothrix cf. agardhii Chroococcus cf. aphanocapsoides Planktothrix rubescens Chroococcus dispersus Planktothrix sp. Chroococcus limneticus Chroococcus minimus Order: Pseudanabaenales 8/9 Chroococcus minutus Leptolyngbya sp. Yes Chroococcus sp. Limnothrix redekei Chroococcus turgidus Planktolyngbya circumcreta Yes Coelosphaerium dubium Planktolyngbya contorta Yes Coelosphaerium kuetzingianum Planktolyngbya limnetica Yes Coelosphaerium sp. Planktolyngbya sp. Yes Dactylococcopsis fascicularis Pseudanabaena galeata Yes* Gomphosphaeria aponina Pseudanabaena limnetica Yes* Gomphosphaeria lacustris Pseudanabaena sp. Yes* Gomphosphaeria sp. Gomphosphaeria virieuxii Order: stocales 19/2 Merismopedia elegans Anabaena circinalis Yes Merismopedia glauca Anabaena flos-aquae Yes Merismopedia hyalina Anabaena helicoidea Yes Merismopedia sp. Anabaena limnetica Yes Merismopedia tenuis Anabaena planctonica Yes Merismopedia tenuissima Anabaena sp. Yes Microcrocis sp. Anabaena spiroides Yes Microcystis aeruginosa Anabaena viguieri Yes Microcystis flos-aquae Anabaenopsis circularis Yes Microcystis incerta Anabaenopsis elenkinii Yes Microcystis sp. Anabaenopsis sp. Yes Snowella lacustris Aphanizomenon elenkinii Yes Woronichinia karelica Aphanizomenon flos-aquae Yes Supplementary material to: Harris et al. (216). Inland Waters 6pp DOI: /IW

3 Supplementary tables S1 & S2 and supplementary figures 1 & 2. Harris et al. (216). Combined effects of nitrogen Genus species N 2 -fixer? Genus species N 2 -fixer? Order: Synechococcales 1/6 Aphanizomenon sp. Yes Cyanogranis basifixa Cylindrospermopsis raciborskii Yes Rhabdoderma irregulare Cylindrospermopsis sp. Yes Rhabdoderma lineare Cylindrospermum cf. musicola Yes Rhabdoderma sigmoidea Cylindrospermum sp. Yes Synechococcus sp. Yes* Raphidiopsis curvata Synechocystis aquatilis Supplementary material to: Harris et al. (216). Inland Waters 6pp DOI: /IW

4 Supplementary tables S1 & S2 and supplementary figures 1 & 2. Harris et al. (216). Combined effects of nitrogen Cheney Cyanobacterial biovolume (%) Cyanobacterial N 2 fixer (%) TN:TP (a) Eagle Creek (b) Cyanobacterial biovolume (%) Geist Morse (c) (d) TN:TP ratio Supplemental Fig. S1. Temporal dynamics of relative cyanobacterial biovolume, relative cyanobacterial N 2 - fixer biovolume, and the TN:TP ratio (by mass) in the 4 study reservoirs used in the study. Numbers on the x- axis represent the last 2 digits of the year post 2 for (a) Cheney and (b d) Eagle Creek, Geist, and Morse, respectively. Supplementary material to: Harris et al. (216). Inland Waters 6pp DOI: /IW

5 Supplementary tables S1 & S2 and supplementary figures 1 & 2. Harris et al. (216). Combined effects of nitrogen 3.5e+7 Cyanobacterial biovolume (µm 3 /ml) 3.e+7 2.5e+7 2.e+7 1.5e+7 1.e+7 5.e+6 (a) (b) TN:TP NO 3 : NH 3 Supplemental Fig. S2. The relationship between cyanobacterial biovolume and the (a) TN:TP and (b) NO 3 :NH 3 ratio. Supplementary material to: Harris et al. (216). Inland Waters 6pp DOI: /IW