Microorganisms in the brewery:
|
|
- Jacob Clarke
- 5 years ago
- Views:
Transcription
1 Nebraska Grower and Brewery Conference January 5-6, 2017 Microorganisms in the brewery: From Acetobacter to Zymomonas and most everything in-between Bob Hutkins
2 The beer microbiome
3 The brewery microbiome
4 For microbiologists, each brew is a microbiology experiment 1. Medium preparation 2. Inoculation and incubation 3. Separate organisms from medium 4. Aseptic packaging
5 Why brewers should care about brewery microorganisms: 1. Safety 2. Compliance 3. Quality 4. Opportunity
6 Manufacturing Safety Accidents Physical, chemical and biological hazards Product Allergens Microbiological
7 Compliance GMPs, HACCP, FDA, and FSMA All food producers must register with the FDA This includes breweries
8 Compliance GMPs, HACCP, FDA, and FSMA All food producers must register with the FDA This includes breweries This means that breweries must have GMPs FSMA is also headed your way
9 Good Manufacturing Practices (GMPs) Good Manufacturing Practices for Craft Brewers (GMPCB), developed by the Brewers Association) GMPs consists of SOPs and other minimum requirements that all food manufacturers must meet to assure products are of high quality and do not pose any risk to the consumer or public. GMPs are prerequisite for HACCP GMP compliance is required (or else)
10 Good Manufacturing Practices ensure products are safe in the Brewery Why? ensure a clean and safe environment for employees ensure sanitary conditions are in place. reduce risks associated with pest infestations reduce risks associated with public health hazards reduce all other risks that could harm a business (including being shut down or action by civil suits)
11 Good Manufacturing Practices ensure products are safe in the Brewery Why not? ensure a clean and safe environment for employees ensure sanitary conditions are in place. reduce risks associated with pest infestations reduce risks associated with public health hazards reduce all other risks that could harm a business (including being shut down or action by civil suits)
12 Implementing Good Manufacturing Practices Use quality raw materials Perform lab tests that can be validated and/or trusted Establish SOPs Educate and train staff Recognize and account for deviations in product quality Create a good quality management system Package and label the product according to guidelines Meet (or exceed) sanitary and processing requirements
13 The beer and brewery microbiome Who is there, from where do they come, and what are they doing?
14 The beer and brewery microbiome 1. Who is there? yeast Saccharomyces non-saccharomyces Brettanomyces Kloeckera Pichia lactic acid bacteria Lactobacillus Pediococcus acetic acid bacteria Enterobacteriacae Staphylococci wild yeast and fungi Zymomonas
15 Lactic acid bacteria A C B 5.0 µm D E D A, Lactobacillus delbrueckii; B. Lactobacillus brevis; C, Pediococcus pentosaceus; D, Pediococcus damnosus; E, Lactobacillus helveticus
16 Genera of lactic acid bacteria Aerococcus Carnobacterium Enterococcus Lactobacillus Lactococcus Leuconostoc Oenococcus Pediococcus Streptococcus Tetragenococcus Vagococcus Weissella
17 Lactic acid bacteria important in fermented foods Lactobacillus Lactococcus Leuconostoc Oenococcus Pediococcus Streptococcus Tetragenococcus
18 Lactic acid bacteria important in beer Lactobacillus Pediococcus
19 Diagnostic characteristics of lactic acid bacteria Fermentative Low mol% G + C Non-sporeforming Gram positive rods and cocci Facultative anaerobes Catalase negative Non-motile Acid-tolerant
20 Practical characteristics of beer LAB Homofermentative (e.g., Pediococcus damnosus) Heterofermentative (e.g., Lactobacillus brevis) Hop tolerance Ethanol tolerance Produce diacetyl Produce biofilms Produce biogenic amines
21 The beer and brewery microbiome 2. From where do they come? ingredients malt hops water yeast adjuncts flavors environment air, water equipment personnel kettles, fermenters, tanks, fillers worker hands, clothing
22 The brewery microbiome
23 Source of microorganisms in the brewery Ingredients barley Fusarium toxins gushing malt hops yeast water air/gas LAB, fungi important during steeping and germination important for antimicrobial activity, not as a source of m/o source of starter strains, but also contaminants
24 Source of microorganisms in the brewery Equipment milling, mashing, lauter tun kettle and whirlpool separator fermentor maturation, bright tanks packaging
25 !!!!!!!!!!!!!!!!!!!!! Potential sources of microbiological contamination (!) during the brewing process. Vaughan et al., J. Inst. Brew. 111:
26 Abundance of micoorganisms in the brewery Source: Bokulich et al. elife 2015;4:e04634
27 Distribution of bacteria in the brewery Source: Bokulich et al. elife 2015;4:e04634
28 The beer and brewery microbiome 3. What are they doing? yeasts anaerobic maltose, other sugars ethanol + CO fermentation 2 glycerol diacetyl phenolics acetic acid acetaldehyde butanediol esters, higher alcohols amino acids H 2 S, SO 2, other sulfites
29 The beer and brewery microbiome 3b. What are they doing? bacteria maltose, other sugars anaerobic fermentation lactic acid diacetyl LAB aerobic oxidation acetic acid AAB amino acids biogenic amines LAB
30 Microbiological quality control in the brewery Same principles apply for beer as for any other perishable food Thus, for beer (and other fermented foods), it s a very thin line between fermented and spoiled
31 Spoilage versus Success: a matter of control Exert control by: Acidity and ph Temperature Moisture Salt Substrate availability
32 In beer, control is by: Low ph Temperature Ethanol Hops Absence of oxygen Substrate availability
33 Beer spoilage should be rare, because: low ph, high ethanol, iso-alpha acids beer is attenuated, few nutrients anaerobic, aerobes cannot grow kettle boil (kill step) stainless steel everywhere But: acid-, ethanol-, and hop-tolerant organisms exist in nature, including breweries wort is nutrient-rich exposure not insignificant (with plenty of opportunities for contamination
34 Beer spoilage organisms: from field to glass barley in field/ malting Aspergillus Fusarium Adapted from Vaughan et al., J. Inst. Brew. 111: mashing/ wort separation Pediocuccus Klebsiella Bacillus Citrobacter fermentation Pediococcus Selenomonas Zymophilus Obesumbacterium stabilization/ packaging Pediocuccus Lactobacillus Pectinatus Megasphaera Zymomonas Pectinatus Micrococcus Megasphaera Pediocuccus Lactobacillus finished beer Acetobacter Gluconacetobacter Pediocuccus Lactobacillus dispense
35 Controlling microbial defects in beer defect diacetyl lactic acid acetic acid sulfur haze cause Pedicococcus Lactobacillus lactic acid bacteria (LAB) acetic acid bacteria (AAB) LAB, AAB, others LAB, yeasts control cleaning sanitation GMP cleaning sanitation GMP cleaning sanitation GMP cleaning sanitation GMP cleaning sanitation GMP
36 Other strategies to prevent microbial defects mash acidification acid addition lactic acid fermentation
37 Viable counts of lactic acid bacteria during a typical lager mash LAB cfu per ml Vaughan et al., J. Inst. Brew. 111:
38 Other strategies to prevent microbial defects mash acidification fermentation packaging/distribution acid addition lactic acid fermentation alpha acid-rich hops pasteurization filtration
39 There are always new trouble-makers Pectinatus and Megasphaera strict anaerobes offensive rotten egg aroma extensive turbidity problematic in late stages of brewing Hard-to-culture LAB Viable But Not Culturable (VBNC) Hop-resistant LAB
40 Strategies for preventing microbial spoilage (works for food, beer, anything) 1. Keep out (asepsis) 2. Remove them 3. Prevent/delay growth 4. Kill/destroy 5. Combinations (Hurdles)
41 Hurdle Concept in Beer organism A organism B ethanol hops ph Eh T
42 Synergistic Effect of Hurdles ethanol hops ph Eh T ethanol hops ph Eh T
43 !!!!!!!!!!!!!!!! Filler!!!!! Potential sources of microbiological contamination (!) during the brewing process. Vaughan et al., J. Inst. Brew. 111:
44
45 Contamination hotspots: Fitting into tight spots Beer Bacteria LAB AAB Yeast
46 The filling station is critically important, as this is where beer is especially vulnerable to contamination This short gap, both in space and time, is sufficient for all sorts of organisms to gain access
47 Surveying for microbes in the brewery 1. Microscopic 2. Cultural 3. Indirect 4. Molecular
48 Microscopy for counting: Easy, informative, inexpensive
49 Cultural Can be qualitative or quantitative Can be general purpose or group-specific These depend on the methods and media Selective v. Non-selective Detection v. Enumeration Detection v. Identification MRS UBA
50 Traditional Methods Also informative, but can take 3 5 days
51 Rapid, qualitative tests ATP Luminescence Can be used for rinse water or equipment (swabs) Easy, rapid, informative Nearly real-time Relative Light Units (RLU) can be scored as pass/fail
52 Source: Hill, A Brewing Microbiology: Managing Microbes, Ensuring Quality and Valorising Waste
53 Modern methods
54
55 ID to species level
56 How to know, before it s too late, that you have a problem? This is the key reason for having a HACCP plan. HACCP, after all, is intended to prevent problems Thus, regular assessment of ingredients, equipment, and processing is the best way to minimize risks of contamination
57
58
59 Consider the following scenarios: What is the tolerance for a mistake? Soldiers deployed to Afghanistan with three week rations Shuttle astronauts at the Space Station for 3 months Caterer to Olympic Athletes in Rio Infant food manufacturer New craft brewer with investors Common goal = zero defects
60
61 Sanitation basics brewing = 90% cleaning + 10% paperwork HACCP Hygiene in the brewery choice of cleaners and sanitizers dealing with biofilms and chronic problems Assessment and testing
62
63
64 Biofilms in the brewery Biofilms can form on equipment, even stainless steel Bacteria within biofilms are more resistant to heat, antimicrobials, and cleaning and sanitizing agents Biofilms will continue to shed bacteria into the food
65
66 Lambic, coolhouse and sour beers Perhaps the original beer produced by humans Initiated by spontaneous or wild fermentation In Belgium, lambics are seasonal, fall to spring This is because the boiled wort needs to cool overnight to about 70 F in a shallow open vessel called a koelschip The U.S. version, called coolship ale, mimics the traditional lambic process
67 Microbiology of lambic-style beers Fermentation is a classic ecological succession One group makes conditions right for the next Four general distinct, but overlapping phases: 1. Enterobacteriacae 2. Primary alcoholic fermentation 3. Acid fermentation 2. Attenuation
68 ACA fermentation profile yeast bacteria ph plato Bokulich et al., PLOS ONE
69 Bacterial diversity of traditional spontaneously fermented lambic beer Spitaels et al., PLOS ONE
70 Yeast diversity of traditional spontaneously fermented lambic beer Spitaels et al., PLOS ONE
71 Brewhouse microbiota are responsible for fermentation of American Coolship Ale Bokulich et al., PLOS ONE
72 Questions
When Good Bugs Go Bad Detection of Beer Spoiling Microorganisms in a Mixed Fermentation Environment
When Good Bugs Go Bad Detection of Beer Spoiling Microorganisms in a Mixed Fermentation Environment Kate Steblenko Jack s Abby Brewing The Beginning Established 2011 Volunteer staff 5,000 sq feet 100 BBLs
More informationGetting To Know Your Lacto. Josh Armagost and Dan Ramos The Brewing Science Institute 2016 Rocky Mountain Micro-Brewers Symposium
Getting To Know Your Lacto Josh Armagost and Dan Ramos The Brewing Science Institute 2016 Rocky Mountain Micro-Brewers Symposium Overview What Is Lacto? Uses in the food industry Metabolism Uses in brewing
More informationAnalysing the shipwreck beer
Analysing the shipwreck beer Annika Wilhelmson, John Londesborough and Riikka Juvonen VTT Technical Research Centre of Finland Press conference 10 th May 2012 2 The aim of the research was to find out
More informationSOUR WORTING. Rick Seibt 1/4/2016
SOUR WORTING Rick Seibt 1/4/2016 Definition Creating sour wort by innoculating wort with souring bacteria, prior to standard beer production (boiling & fermentation). More commonly known as Kettle Souring.
More informationIntro to Professional Brewing Quality Assurance. Rick Blankemeier Quality Assurance Manager Stone Brewing Co
Intro to Professional Brewing Quality Assurance Rick Blankemeier Quality Assurance Manager Stone Brewing Co What is Quality? Quality the degree of excellence of something Keeping our beer consistently
More informationyeast-derived flavours
yeast-derived flavours Positive flavour in some beers - offflavour in others Produced by yeast during fermentation Critically affected by wort [Zn] and yeast health Can also be produced by contaminant
More informationChoosing the Right Yeast
San Diego California June, 2011 Choosing the Right Yeast Chris White and Jamil Zainasheff Yeast Chapters Part One: The Importance of Yeast and Fermentation Part Two: Biology, Enzymes, and Esters Part
More informationVWT 272 Class 15. Quiz Number of quizzes taken 25 Min 6 Max 30 Mean 24.0 Median 26 Mode 30
VWT 272 Class 15 Quiz 13 14 Number of quizzes taken 25 Min 6 Max 30 Mean 24.0 Median 26 Mode 30 Class 15 Bacteria: the Good, the Bad, and the Ugly What you see is that the most outstanding feature of life's
More informationMICROBES MANAGEMENT IN WINEMAKING EGLANTINE CHAUFFOUR - ENARTIS USA
MICROBES MANAGEMENT IN WINEMAKING EGLANTINE CHAUFFOUR - ENARTIS USA WEBINAR INFORMATION 35 minute presentation + 10 minute Q&A Save Qs until the end of the presentation Use chat box for audio/connection
More informationilocos region Food Safety Team
6/26/2012 1 1 Food Hazards Employee Training Health, Hygiene & Hand Washing Cross Contamination Food Preparation Process Controls 6/26/2012 2 2 To understand the basic knowledge of Good Manufacturing Practices
More informationJuice Microbiology and How it Impacts the Fermentation Process
Juice Microbiology and How it Impacts the Fermentation Process Southern Oregon Wine Institute Harvest Seminar Series July 20, 2011 Dr. Richard DeScenzo ETS Laboratories Monitoring Juice Microbiology: Who
More informationIntroduction to MLF and biodiversity
Introduction to MLF and biodiversity Maret du Toit DEPARTMENT OF VITICULTURE AND OENOLOGY INSTITUTE FOR WINE BIOTECHNOLOGY Stellenbosch University E-mail: mdt@sun.ac.za Microbiology of wine your perpsectives
More informationQualifications. The Certificate in the Fundamentals of Brewing and Packaging of Beer (FBPB) Full Examination Syllabus
Qualifications The Certificate in the Fundamentals of Brewing and Packaging of Beer (FBPB) Full Examination Syllabus Institute of Brewing and Distilling 2011 A qualification for the validation of the training
More informationTHE MICROBIOLOGY OF FERMENTATION. Curt Wittenberg for Society of Barley Engineers August 3, 2016
THE MICROBIOLOGY OF FERMENTATION Curt Wittenberg for Society of Barley Engineers August 3, 2016 Brewer s make wort; Yeast make beer. Where are we going from here? Practical Yeast Biology Yeast growth Fermentation
More informationViniflora Oenos. Product Information. Description. Packaging. Physical Properties. Application. Storage and handling. Version: 7 PI-EU-EN
Description is a freeze-dried pure culture of Oenococcus oeni. It is a heterofermentative malolactic bacteria which has been selected to ensure a fast and safe malolactic fermentation when inoculated directly
More informationLiving Factories. Biotechnology SG Biology
Living Factories Biotechnology SG Biology Learning Outcomes 1 State that the raising of dough and the manufacture of beer and wine depend on the activities of yeast. Identify yeast as a single celled fungus,
More informationRESOLUTION OIV-OENO
RESOLUTION OIV-OENO 462-2014 CODE OF GOOD VITIVINICULTURAL PRACTICES IN ORDER TO AVOID OR LIMIT CONTAMINATION BY BRETTANOMYCES THE GENERAL ASSEMBLY, Considering the actions of the Strategic Plan of the
More informationSPOILAGE OF CANNED PRODUCTS - BIOCHEMICAL, ENZYMATIC AND MICROBIAL SPOILAGE SPOILAGE OF CANNED PRODUCTS
SPOILAGE OF CANNED PRODUCTS - BIOCHEMICAL, ENZYMATIC AND MICROBIAL SPOILAGE SPOILAGE OF CANNED PRODUCTS Enzymatic spoilage Many reactions in plant and animal tissues are activated by enzymes. The changes
More informationIT HAD BETTER NOT BE MY FAULT
IT HAD BETTER NOT BE MY FAULT AN ANALYSIS OF WINE GONE BAD CSU Assoc. Prof. of Enology Stephen Menke WINE QUALITY, GREATNESS, AND FAULTS Not all of us agree on the definitions of wine quality, as it can
More informationENARTIS NEWS WANT TO PRODUCE A WINE WITH LOW OR ZERO SO 2
ENARTIS NEWS WANT TO PRODUCE A WINE WITH LOW OR ZERO SO 2 ADDITION? SO 2 is one of the most controversial additives currently used in the wine industry. Numerous attempts have been made to find alternatives
More informationLACTIC ACID BACTERIA (OIV-Oeno , Oeno )
LACTIC ACID BACTERIA (OIV-Oeno 328-2009, Oeno 494-2012) 1. OBJECT, ORIGIN AND FIELD OF APPLICATION Lactic acid bacteria are used in oenology to perform malolactic fermentation. The lactic acid bacteria
More informationIn pursuit of flavor
In pursuit of flavor A presentation that aims to contribute to a greater understanding of sour beer production. *What s NOT Included? How to make sour beer Too many variables! Kettle sour acid is easy
More informationMICROBIOLOGICAL CONTROL IN THE PROCESS OF BEER PRODUCTION KONTROLLI MIKROBIOLOGJIK NE PROCESIN E PRODHIMIT TE BIRRES
ISSN 2073-2244 www.alb-shkenca.org Copyright Institute Alb-Shkenca AKTET Journal of Institute Alb-Shkenca Revistë Shkencore e Institutit Alb-Shkenca MICROBIOLOGICAL CONTROL IN THE PROCESS OF BEER PRODUCTION
More informationPaper No.: 02. Paper Title: Principles of The food processing & preservation. Module 21: Food Fermentation
Paper No.: 02 Paper Title: Principles of The food processing & preservation Module 21: Food Fermentation Paper Coordinator: Dr. P. Narender Raju, Scientist, National Dairy Research Institute, Karnal, Haryana
More informationMICROBES MANAGEMENT IN WINEMAKING EGLANTINE CHAUFFOUR - ENARTIS USA
MICROBES MANAGEMENT IN WINEMAKING EGLANTINE CHAUFFOUR - ENARTIS USA WEBINAR BASICS Presentation will proceed from beginning to the end without interruption by questions. During the presentation, the chat
More informationBrewing Process all grain
Brewing Process all grain Water treatment Chlorine/Chloramine removal Mineral composition Brewing salts Acid additions Mash PH stabilizer Grist Building Milling Importance of milling Mashing PH Temperature
More information8/05/2017. Fermented beverages as an alternative for soft drinks. Water kefir. Water kefir
Fermented beverages as an alternative for soft drinks Non- and low-alcoholic beverages with acetic acid bacteria as an overlooked contributor ir. Jonas De Roos Studiereeks Micro-Organismen: Part 1 - The
More informationFood Microbiology. Microorganisms in Food Food Preservation Food-borne Illness Fermented Foods
Food Microbiology Microorganisms in Food Food Preservation Food-borne Illness Fermented Foods Microorganisms in Food Factors affecting microbial growth in food composition ph presence and availability
More informationMousiness, Brettanomyces, Cork Taints
Mousiness, Brettanomyces, Cork Taints Thomas Henick-Kling Brett Effect in Wine Loss of fruit, floral aromas Increase in Spice, Smoke Increase in overall complexity Increase in Creosote, Plastic Increase
More informationPractical actions for aging wines
www.-.com Practical actions for aging wines document. Professional use not allowed (training, copy, publication, commercial document, etc.) without written D. s authorization Thirteen main key-points for
More informationCo-inoculation and wine
Co-inoculation and wine Chr. Hansen Fermentation Management Services & Products A definition of co-inoculation Co-inoculation is the term used in winemaking when yeasts (used to manage alcoholic fermentations
More informationFD-DVS Viniflora CiNe Product Information
Description Viniflora CiNe is a freeze-dried pure culture of Oenococcus oeni. It is a heterofermentative malolactic bacteria which has been selected to ensure a fast and safe malolactic fermentation when
More informationWhen life throws you lemons, how new innovations and good bacteria selection can help tame the acidity in cool climate wines
When life throws you lemons, how new innovations and good bacteria selection can help tame the acidity in cool climate wines Dr. Sibylle Krieger-Weber R&D Bacteria, Lallemand Germany VitiNord August 2
More informationYeast- Gimme Some Sugar
Yeast- Gimme Some Sugar Taxonomy: Common yeast encountered in brewing The main cultured brewers yeast is genus Saccharomyces Saccharomyces means sugar fungus S. cerevisiae is ale yeast S. pastorianus is
More informationQualifications. The General Certificate in Brewing (GCB)
Qualifications The General Certificate in Brewing (GCB) Examination Syllabus 2014 Introduction. The General Certificate in Brewing (GCB) gives international recognition of a basic, under-pinning knowledge
More informationBACTERIA USED IN FOOD FERMENTATION
BACTERIA USED IN FOOD FERMENTATION INTORODUCTION ALL ARE CURRENTLY CLASSIFIED IN ONE OF THREE PHYLA ( 門, DIVISION): Proteobacteria: Gram negative bacteria involved in vinegar fermentation Firmicutes: Lactic
More informationAlcohols, Acids, and Esters in Beer. Matt Youngblut BAM Members Meeting October 13th, 2016
Alcohols, Acids, and Esters in Beer Matt Youngblut BAM Members Meeting October 13th, 2016 What are Alcohols, Acids, and Esters? Alcohols Any organic molecule with a hydroxyl group (X~OH) that s attached
More informationPilot Malting and Brewing Trials with 2011 Crop CDC Meredith Barley
2012 Pilot Malting and Brewing Trials with 2011 Crop Barley 7/5/2012 Pilot Malting and Brewing Trails with 2011 Crop Samples of Barley Executive summary CMBTC conducted several pilot malting and brewing
More informationMIC305 Stuck / Sluggish Wine Treatment Summary
Page: 1 of 5 1. BEFORE reinoculating 1.1 Check yeast viability with methylene blue. If < 25 % of yeasts are viable, rack off yeast lees and skip to reinoculation method below. If there are many live cells,
More informationINTRODUCTION probiotics Fermentation
INTRODUCTION Food microbiology is the study of the microorganisms that grow in or contaminate the foods that humans consume. In general, food microbes could be considered either beneficial or a nuisance
More informationAugust Instrument Assessment Report. Bactest - Speedy Breedy. Campden BRI
August 2013 Instrument Assessment Report Campden BRI food and drink innovation Bactest - Speedy Breedy Assessment of the suitability of Speedy Breedy as a rapid detection method for brewing contaminants
More informationSilage Forage Quality Using Inoculants and Packing. Dr. Dan Undersander University of Wisconsin
Silage Forage Quality Using Inoculants and Packing Dr. Dan Undersander University of Wisconsin Inoculants Silage additives whose main ingredients are lactic acid producing bacteria Purpose of Inoculants
More informationDr.Nibras Nazar. Microbial Biomass Production: Bakers yeast
Microbial biomass In a few instances the cells i.e. biomass of microbes, has industrial application as listed in Table 3. The prime example is the production of single cell proteins (SCP) which are in
More informationSour Beer A New World approach to an Old World style. Brian Perkey Lallemand Brewing
Sour Beer A New World approach to an Old World style. Brian Perkey Lallemand Brewing History & Styles of Sour Beers Sour beer styles have existed for centuries What do we mean by Sour beer? History and
More informationACETALDEHYDE High amount of fermentable sugars
ALCOHOLIC overpowering alcohol flavor, acetone, paint thinner, sharp ACETALDEHYDE High amount of fermentable sugars High fermentation temperature green apples, grassy, acetic(vinegar), cidery Underpitching
More informationFresh Beer, Fresh Ideas
123rd MBAA Anniversary Convention Fresh Beer, Fresh Ideas Alastair Pringle Pringle Scott LLC Objective and Outline Objective Identify practical solutions for keeping beer as fresh as possible. Outline
More informationMALOLACTIC FERMENTATION QUESTIONS AND ANSWERS SESSION
MALOLACTIC FERMENTATION QUESTIONS AND ANSWERS SESSION ML SCHOOL September 2016 University Stellenbosch QUESTIONS Why should I care about specific wine lactic acid bacteria? Why should I pay if MLF comes
More informationPRODUCTION OF BEER Page 1
PRODUCTION OF BEER Beer is an alcoholic beverage made from malted grains, hops, yeast and water. The grain is usually barley or wheat. Fruits, herbs and spices may also be used for special styles. The
More informationMicrobial Ecology Changes with ph
Microbial Ecology Changes with ph Thomas Henick-Kling Director, Viticulture & Enology Program Professor of Enology Winemaking Involves Different Population of Microorganisms Kloeckera / Hanseniaspora Schizosaccharomyces
More informationMolecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia)
Molecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia) T. Kuchta1, D. Pangallo2, Z. Godálová1, A. Puškárová2, M. Bučková2, K. Ženišová1, L. Kraková2
More informationPaul Smith 22-Jan-01
Paul Smith 22-Jan-01 1. Assignment Topic Before taking up your duties in a new microbrewery capable of producing up to 50 hl of cask-conditioned beer per week, you have been asked to organize a small laboratory.
More informationInnovations and Developments in Yeast. Karen Fortmann, Ph.D. Senior Research Scientist
Innovations and Developments in Yeast Karen Fortmann, Ph.D. Senior Research Scientist A Little Bit About White Labs Why I m Standing Here in Front of You White Labs Motto Committed to being the best yeast
More informationDRAFT TANZANIA STANDARD
TBS/AFDC 13 (5361) P3 Rev TZS 1245:2010 ICS: 67.160 DRAFT TANZANIA STANDARD Draught beer Specification TANZANIA BUREAU OF STAND TBS 2010 Second Edition 2010 TANZANIA STANDARD TBS/AFDC 13(5361) P3 / Rev.
More informationRaw materials. MALT (Malted cereal, usually barley) WATER
Jelena Stojanovic Some history In Babylonia 6000 B. C first recorded document about beer By 4000 B. C there was 16 different types of beer made from barley and honey Imperial Egypt, 4000 B. C had 4 types
More informationAn overview of beer flavour and sensory training
An overview of beer flavour and sensory training Cara Technology, UK Presented by Tim Godfrey Newlands Spring Brewing Co. 11 August 2016 overview Beer flavour overview Flavours from malt Flavours from
More informationFOOD PRODUCTION - BEVERAGES Demonstrate knowledge of brewhouse operations and wort production
1 of 7 level: 3 credit: 6 planned review date: August 2008 sub-field: purpose: entry information: accreditation option: moderation option: Food and Related Products Processing People credited with this
More informationMalting and Brewing Trials with 2011 Crop Barley Samples of CDC PolarStar and AC Metcalfe
2012 Malting and Brewing Trials with 2011 Crop Barley Samples of and 7/10/2012 Malting and Brewing Trials with 2011 Crop Barley Samples of and Summary Pilot malting and brewing trials were conducted at
More informationPaper 6 Food Biotechnology F06FB24 Fermented Milk Products Curd (Indian Dahi), Buttermilk and Cheese
Paper 6 Food Biotechnology F06FB24 Fermented Milk Products Curd (Indian Dahi), Buttermilk and Cheese 6.24.1 Introduction This module deals with the variety of fermented milk products like curd (Indian
More informationUnit code: A/601/1687 QCF level: 5 Credit value: 15
Unit 24: Brewing Science Unit code: A/601/1687 QCF level: 5 Credit value: 15 Aim This unit will enable learners to apply knowledge of yeast physiology and microbiology to the biochemistry of malting, mashing
More informationYEAST Wrangling The Many Flavors of Brewing Yeast CURT WITTENBERG FOR SOCIETY OF BARLEY ENGINEERS OCTOBER 4, 2017
YEAST Wrangling The Many Flavors of Brewing Yeast CURT WITTENBERG FOR SOCIETY OF BARLEY ENGINEERS OCTOBER 4, 2017 Please distribute cups and beer Please keep beers in numerical order: 1-6. Please do not
More informationA d v a n c e d B a k i n g a n d P a s t r i e s ( 1 2 D )
8 9 9 8 A d v a n c e d B a k i n g a n d P a s t r i e s ( 1 2 D ) 40S/40E/40M A Baking and Pastry Arts Course 8 9 9 8 : A d v a n c e d B a k i n g a n d P a s t r i e s ( 1 2 D ) 4 0 S / 4 0 E / 4
More information01/04/57. Food Microbiology_Katekan Dajanta 1. Microbes used in food industry. Microflora in fermented foods
1 Microbes used in food industry Nature uses microorganisms to carry out fermentation processes, and for thousands of years Fermentation in food processing is the conversion of carbohydrates to alcohols
More informationMicro-brewing learning and training program
Micro-brewing learning and training program (LdV Beer School) Program izobraževanja v mikro-pivovarstvu (projektno gradivo) Beer styles Raw materials Brewhouse technology - mashing and mashing in - lautering
More informationCase Histories: Biocide Interactions and. Tolerance/Resistance to Biocides in the Paper Making Process
Case Histories: Biocide Interactions and Development of Microbial Tolerance/Resistance to Biocides in the Paper Making Process Philip M. Prichard, Ph.D Philip M. Prichard Consulting LLC PaperCon 2011 Page
More informationCase Study I Soy Sauce. Scenario:
Case Study I Soy Sauce. Scenario: Brewing soy sauce is one of the original biotech industries. Soy sauce was shipped in barrels within Asia over 500 years ago, and in bottles to Europe by the 1600s. Now
More informationPractical management of malolactic fermentation for Mediterranean red wines
Practical management of malolactic fermentation for Mediterranean red wines Author : Dominique DELTEIL, ICV This article presents the main points discussed in a paper presented by D. Delteil at a Lallemand
More informationHACCP. Hazard Analysis Critical. For The Food Service Worker
ALABAMA A&M AND AUBURN UNIVERSITIES HE-726 HACCP For The Food Service Worker Hazard Analysis Critical Control Point (HACCP) is a systematic approach to food production as a means of assuring food safety.
More informationRapid detection and enumeration of spoiler microorganisms from beer mixes with low or 0.0 alcohol content
Rapid detection and enumeration of spoiler microorganisms from beer mixes with low or 0.0 alcohol content Marta Orive Camprubí, EBC Symposium 2014, Wien BIOLOGICAL STABILITY: Ecological aspects +++ high
More informationReal-time PCR beer screening
Real-time PCR beer screening For a quick and GEN-IAL result For yeast propagation, in-process control and/or for online final product control Avoid product recalls Reduce the risk of spoilage R-Biopharm
More informationStuck / Sluggish Wine Treatment Summary
800.585.5562 BSGWINE.COM 474 Technology Way Napa, CA 94558 Stuck / Sluggish Wine Treatment Summary 1. BEFORE REINOCULATING 1.1 Check yeast viability with methylene blue. Mix a sample of must with an equal
More informationMicrobial Faults. Trevor Phister, PhD Assistant Professor
Microbial Faults Trevor Phister, PhD Assistant Professor Overview Wine microbiology Microbial faults Brettanomyces Lactic acid bacteria Cork Taint Controlling microbial faults Sanitation Quality programs
More informationHACCP for Food Safety:
HACCP for Food Safety: Asia-Pacific Economic Cooperation Partnership Training Institute Network Warren Stone, MBA Grocery Manufacturers Association Washington, DC November 6, 2010 Workshop Objectives Introduce
More informationPaper 6 Module 25 Fermentation of Fish and Meat [Year] Paper 6 Food Biotechnology F06FB25 Fermentation of Fish and Meat
Paper 6 Food Biotechnology F06FB25 Fermentation of Fish and Meat 6.25.1 Introduction This module deals with the method of preserving fish and meat so as to retain their colour, flavour and texture and
More informationBENEFITS OF DANISCO KEFIR CULTURES
T M 2 0 8 2-1 e Danisco kefir cultures Kefir grains INTRODUCTION Danisco kefir cultures make it possible to produce traditional kefir as it has been known for centuries. Securing exactly the right characteristics
More informationStreamlining Food Safety: Preventive Controls Brings Industry Closer to SQF Certification. One world. One standard.
Streamlining Food Safety: Preventive Controls Brings Industry Closer to SQF Certification One world. One standard. Streamlining Food Safety: Preventive Controls Brings Industry Closer to SQF Certification
More informationAN OVERVIEW OF THE BREWING PROCESS. Jared Long Head Brewer Altitude Chophouse and Brewery
AN OVERVIEW OF THE BREWING PROCESS Jared Long Head Brewer Altitude Chophouse and Brewery jared@altitudechophouse.com Water Objective: understand your water, but don t obsess over it. ph alone doesn t
More informationBrettanomyces prevention
Brettanomyces prevention Use SO 2 at crush Sanitize or sterilize new barrels Clean surfaces and containers thoroughly Employ microbial monitoring Test all barrels and tanks initially and periodically Filter
More informationViniflora CH11 Product Information
Description Viniflora CH11 is a freeze-dried culture of Oenococcus oeni. It is a heterofermentative malolactic bacteria which has been selected to ensure a fast and safe malolactic fermentation when inoculated
More informationEntry Level Assessment Blueprint Retail Commercial Baking
Entry Level Assessment Blueprint Retail Commercial Baking Test Code: 4010 / Version: 01 Specific Competencies and Skills Tested in this Assessment: Safety and Sanitation Identify causes and prevention
More informationInfluence of yeast strain choice on the success of Malolactic fermentation. Nichola Hall Ph.D. Wineries Unlimited, Richmond VA March 29 th 2012
Influence of yeast strain choice on the success of Malolactic fermentation Nichola Hall Ph.D. Wineries Unlimited, Richmond VA March 29 th 2012 INTRODUCTION Changing conditions dictate different microbial
More informationSTABILIZATION OPTIONS. For Sweet Wines before Bottling
STABILIZATION OPTIONS For Sweet Wines before Bottling Sugar-Sugar Top source of carbon Excellent seller of wine Brings balance to wine with high acidity/astringency Promotes peace, comfort and wellbeing
More informationFermenting on the Flip Side
Fermenting on the Flip Side Mary Izett Co-host of Fuhmentaboudit! on Heritage Radio Network Urban Brewing & Alternative Fermentations: Techniques & beverages for anyone short on space and time but long
More informationIsolating WILD. Yeast Strains. By Mike Lentz ZYMURGY JAzym14_REFwildyeast.indd 54
Isolating WILD Yeast Strains By Mike Lentz 54 54-60 JAzym14_REFwildyeast.indd 54 EDITOR S NOTE: This is the third published experiment from the AHA s Research & Education Fund. For more on the REF and
More information2014 Crop Merit 57 Pilot Malting and Brewing Trials
2014 Crop Merit 57 Pilot Malting and Brewing Trials 3/27/2015 2014 Crop Merit 57 Pilot Malting and Brewing Trials Summary CMBTC conducted a pilot malting and a pilot brewing trial with a 2014 crop barley
More informationMAKING WINE WITH HIGH AND LOW PH JUICE. Ethan Brown New Mexico State University 11/11/2017
MAKING WINE WITH HIGH AND LOW PH JUICE Ethan Brown New Mexico State University 11/11/2017 Overview How ph changes during winemaking Reds To adjust for high ph and how Whites Early harvest due to poor conditions
More informationChristian Butzke Enology Professor.
Christian Butzke Enology Professor butzke@purdue.edu www.indyinternational.org www.indianaquality.org SO 2 & Sorbate Management Oxygen Management Skin Contact Time Residual Nutrients Temperature, ph &
More informationHow yeast strain selection can influence wine characteristics and flavors in Marquette, Frontenac, Frontenac gris, and La Crescent
How yeast strain selection can influence wine characteristics and flavors in Marquette, Frontenac, Frontenac gris, and La Crescent Katie Cook, Enologist, University of Minnesota Fermentation Yeast Saccharomyces
More informationChair J. De Clerck IV. Post Fermentation technologies in Special Beer productions Bottle conditioning: some side implications
Chair J. De Clerck IV Post Fermentation technologies in Special Beer productions Bottle conditioning: some side implications Chair J. De Clerck XIV, september 14 Bottle conditioning: some side implications
More informationMicrobiologist. QA Lab at Boulevard Brewing Co. Production QA Member of A.S.B.C. Sensory Specialist, Beer Judge
Microbiologist Jennifer Helber Lab Science, Etc. QA Lab at Boulevard Brewing Co. Production QA Member of A.S.B.C. Sensory Specialist, Beer Judge Consultant Lab Science, Etc. K.C. Beer Pairing Examiner
More informationHACCP SYSTEMS EXPLAINED
Chapter 2 HACCP SYSTEMS EXPLAINED 1.0 WHAT IS HACCP? 2.0 FOOD SAFETY HAZARDS 2.1 Assessing Hazards 2.2 Cross-contamination 3.0 REQUIREMENTS FOR A HACCP SYSTEM 3.1 Management Commitment 3.2 Selecting the
More informationDETECTION OF CAMPYLOBACTER IN MILK A COLLABORATIVE STUDY
DETECTION OF CAMPYLOBACTER IN MILK A COLLABORATIVE STUDY EURL-Campylobacter workshop 2018 Hanna Skarin CAMPYLOBACTER IN MILK Campylobacter spp. - in the intestine of healthy cattle Risk for fecal contamination
More informationBerliners and Beyond: Sour Mashing and Its Applications. Derek Springer National Homebrewers Conference 2015/ 06/ 13
Berliners and Beyond: Sour Mashing and Its Applications Derek Springer National Homebrewers Conference 2015/ 06/ 13 Vegan Warning! Sample #1: Extract Berliner 23A. Berliner Weisse 100% Bavarian Wheat DME
More informationFood Science and Technology Notes
Food Science and Technology Notes Extension Division Deportment of Food ScienceandTechnology Virginia Polytechnic Institute Blacksburg, Virginia SEP - - 197~ HOMEMADE CULTURED MILK PRODUCTS by P. M. Large,
More informationAdvanced Yeast Handling. BFD education Kai Troester
Advanced Yeast Handling BFD education Kai Troester Agenda Why yeast storage Short term Long term Yeast Harvesting Yeast washing Sterile techniques Yeast propagation Equipment Why yeast storage Yeast is
More informationEvaluation of the Malting and Brewing Performance of the New Canadian Malting Barley Variety Norman
Evaluation of the Malting and Brewing Performance of the New Canadian Malting Barley Variety Norman Summary Malting Performance Water Uptake Good Chitting at end of Steep Good Acrospire Growth Good Malt
More informationCERTIFICATE OF ACCREDITATION
CERTIFICATE OF ACCREDITATION ANSI-ASQ National Accreditation Board 500 Montgomery Street, Suite 625, Alexandria, VA 22314, 877-344-3044 This is to certify that Biogen Laboratory Developments, LLC Food,
More informationSCENARIO Propose a scenario (the hypothesis) for bacterial succession in each type of milk:
Prokaryotic Diversity! and Ecological Succession in Milk Name INTRODUCTION Milk is a highly nutritious food containing carbohydrates (lactose), proteins (casein or curd), and lipids (butterfat). is high
More informationThis place covers: Raw materials used in preparing beer (e.g. malt, hops), and treatment thereof.
CPC - C12C - 2017.08 C12C BREWING OF BEER (cleaning of raw materials A23N; pitching and depitching machines, cellar tools C12L; propagating yeasts C12N 1/14; non-beverage ethanolic fermentation C12P 7/06)
More informationREDUCING SULPHITES CONTENT IN WINES
REDUCING SULPHITES CONTENT IN WINES Consumers and sulphites in wine Roles and impacts of SO 2 in Oenology Bacteria Yeast Oxygene, quinones Tyrosinase, laccase Antiseptic Antioxidant Antioxidasic Oxidised
More informationFERMENTATION. By Jeff Louella
FERMENTATION By Jeff Louella Why Understand Fermentation? Understanding the science behind fermentation can greatly affect the quality of beer made. There are some great products on the market to help
More information