ZAIKA I.V. 1, SOZINOV A.A. 2, 3, KARELOV A.V. 2, KOZUB N.A. 2, FILENKO A.L. 4, SOZINOV I.A. 2 1

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1 11. McNeil M.D., Kota R., Paux E., Dunn D., McLean R., Feuillet C., Li D., Kong X., Lagudah E., Zhang J.C., Jia J.Z., Spielmeyer W., Bellgard M., Apples R. BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs // Mol. Breeding, V. 22. P ,..,..,..,..,..,.. - cssr2 //... «:» ( ), ,..,..,..,..,..,..,..,..,.. - Lr34 // , ZAIKA I.V. 1, SOZINOV A.A. 2, 3, KARELOV A.V. 2, KOZUB N.A. 2, FILENKO A.L. 4, SOZINOV I.A. 2 1 National Science Center «Institute of Agriculture NAAS» Ukraine, 08162, Kiev region, Kievo-Sviatoshinskij district, Tchabany, Mashinobudivnikiv str., 2b, zaika-@mail.ru 2 Institute of plant protection NAAS Ukraine, 03022, Kiev, Vaselkivska str., 33, sia1@i.com.ua 3 Institute of food biotechnology and genomics Ukraine, 04123, Kiev, Osipovskogo, 2a 4 Laboratory of guiding biotechnology «Neogene» Ukraine, 04112, Kiev, Oleni Teligi, 4, info@neogene.com.ua POLYMORPHISM OF THE MODERATE NON-RACE-SPECIFIC DISEASE RESISTANCE GENE Sr2/Lr27 AND Lr34/Yr18/Pm38 IN BREAD WHEAT CULTIVARS OF NSC «INSTITUTE OF AGRICULTURE NAAS» BREEDING Aims. The object of our investigation is allelic state identification of Sr2/Lr27 and Lr34/Yr18/Pm38 gene in bread wheat cultivars of Polissia. Methods. Allelic state of the Lr34 gene was identified with the moleculargenetic marker caisbp1 use and marker for Sr2/Lr27 locus was cssr2. Results. In five cultivars (13,5 % of the total number) was detected the «Marquiz-allele» and rest had the «null allele» of Sr2 locus. The «resistant» allelic state of Lr34 gene (Lr34+) was identified in 11 cultivars (39 %). The «susceptible» allelic state of Lr34 was identified in 9 wheat cultivars (32 %) and 8 (29 %) cultivars showed polymorphism at the Lr34 locus. Conclusions. It is necessary to involve in wheat breeding process the Lr34 and Sr2 gene, because it is valuable source of disease resistance. Key words: stem rust, Ug99, bread wheat, resistance, Sr2, Lr ,.. 1,.. 1,.. 2,.. 3,.. 1,.. 2, , ,,., 3,; olgmur1@yandex.ru 2, ,,., 27, v.lemesh@igc.bas-net.by , ,,.,, vik_volovik@mail.ru (Brassica napus oleifera D.C.) (Brassicaceae). (B.napus oleifera annua Metzg.) (B. napus oleifera biennis Metzg.). (2n=38, ) - 44

2 ., - (Brassica oleracea L., 2n=18, - ) - (Brassica rapa L., 2n=20, A- ) c - [10]. - :, -, [1]. - ( - ).,, -,. 13,, (. - ) «-» (., ) (.). -, -. - DAPI - (1,53 3,3 ) [4] - - [8]. FISH- [6, 9, 5]., DAPI- 26S 5S., FISH 26S 5S, - [3, 7]. Olimpus BX61 - CoolSnap («Roper ScientificInc», C ). 38, -, - [8] - - (,..).,., Olin-Fatih Heneen (1992),,, : (7 ), (6 ), 6 (6 ). - C-. C-,,, -. - [7]. - 45,, - -9 (9- ), - ( ). -, - -., 14,, -, - -., - - [2]., -, C., - ( 8 10 ), -,

3 -. -, -. FISH 26S 5S. - FISH - DAPI-, -. -, 26S / 5S,.. FISH 26S 5S S 26S , S S, S S S S, S, 14 5S. 26S 5S -, - 4, 5, 6, 8, 10, 14, 15, ,. - 5S S 5S , - 4, 5, 6, S. 46

4 ., - 26S, 5S, 3 [6, 9, 5, 11] 4 [5] S, - 5S., - 26S 5S S. - ( 5 7 ), -.,, - - 9, -, -,. FISH, ( - ) 5S 26S -., 16 26S, -, -,., S 26S. - ( ). (,, ) 26S 4, 5S 26S., - 26S 5S - (.), S 5S, -. 26S 16., - -, 13,, S 5S. - / , «,» // ,..,... Macleaya cordata (Willd.) R. Br. // ,..,..,.. Adenolinum Stellerolinum in situ (FISH) // , Hasterok R., Maluszynska J. Cytogenetic markers of Brassica napus chromosomes // Journal of Applied Genetics. 47

5 2000. Vol. 41. P Hasterok R., Wolny E., Hosiawa M. et al., Comparative analysis of rdna distribution in chromosomes of various species of Brassicaceae // Ann. Bot V. 97. P Kamisugi.Y, Nakayama S., O Neil C.M. et al. Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes // Plant Molecular Biology Vol. 38. P Muravenko O.V., Yurkevich O.YU.,Bolsheva N.L. et al, Comparison of genomes of eight species of sections Linumand Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis // Genetika V. 135, 2. P Olin-Fatih M. and Heneen W.K. C-banded karyotypes of Brassica campestris, B. oleracea and B. napus // Genome Vol. 35. P Snowdon R. J., Friedrich T., Friedt W., Kohler W. Identifying the chromosomes of the A- and C-genome diploid Brassica species B. rapa (syn. campestris) and B. oleracea in their amphidiploid B. napus // Theor. Appl. Genet Vol P U N. Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization // Japanese Journal of Botany Vol. 7. P Xiong Z. and Pires J.C. Karyotype and identification of all homoelogous chromosomes of allopolyploid Brassica napus and its diploid progenitors // Genetics Vol P ZEMTSOVA L.V. 1, AMOSOVA A.V. 1, SAMATADZE T.E. 1, GRUSHETSKAYA Z.E. 2, VOLOVIK V.T. 3, ZELENIN A.V. 1, LEMESH B.A. 2, MURAVENKO O.V. 1 1 Engelgardt Institute of Molecular Biology of RAS Russia, , Moscow, Vavilov str., 32, olgmur1@yandex.ru 2 The Institute of Genetics and Cytology of NAS of Belarus Belarus, , Minsk, Academichnaya str., 27, v.lemesh@igc.bas-net.by 3 All-Russian Williams Fodder Research Institute of RAAS Russia, , Lobnya, Moscow region, vik_volovik@mail.ru CHROMOSOME MARKERS STUDY OF RAPE VARIETIES OF RUSSIAN AND BELORUSSIAN SELECTION Aims. Dependable marking of rape chromosomes is necessary for breeding of agriculturally important varieties. Using chromosome markers the karyotypes of 13 spring and winter rape varieties of Russian and Belorussian selection were studied. Methods. C-, DAPI-banding and FISH with 26S and 5S rdna were used. Results. More informative C/DAPI-banding was obtained by using DNA intercalator 9-aminoacridine, and rape chromosome identification was made. 26S and 5S rdna loci were localized on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intra- and intervarietal polymorphism of these chromosome markers was found. Conclusions. The generalized species karyogram that included all the alternates of C/DAPI-patterns as well as 26S and 5S rdna localization was constructed. Key words: rape varieties, chromosome markers, polymorphism...,..,..,.., ,.,, 71, insect@anrb.ru -3 - ( - ) (9- ) - [1, 2].,.,,, -, : - -,. 48

ALTHOUGH whole-genome duplications (polyploidy)

ALTHOUGH whole-genome duplications (polyploidy) Copyright Ó 2011 by the Genetics Society of America DOI: 10.1534/genetics.110.122473 Karyotype and Identification of All Homoeologous Chromosomes of Allopolyploid Brassica napus and Its Diploid Progenitors