Basic beer style parameters Quality Control by basic means

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1 EBC Symposium SEP 2016, Wroclaw Basic beer style parameters Quality Control by basic means Axel G. Kristiansen Deputy Director DTU Diplom Danish Technical University

2 This presentation serves to put Quality as a disciplin on the agenda for small breweries as the Quality function is some times perceived: Requiring special staff Time consuming Costly Claim 1: Quality Control can be implemented rather cheaply. Claim 2: But missing quality however can be costly! 2

3 The Quality Hierarchy of documentation

4 Useful Terminology for any brewmaster Sample Plan Recipe Process Description Analysis Specification List os samples taken during process: The sampling plan is maybe the most important element of the Quality System! List of all ingredients incl. amounts. Does not explain the manufacturing process. Manufacturing guide to produce a defined amount of product on a defined plant. A laboratory s measurements of contents of certain contents in the product or in raw materials. Does not explain the used recipe or process. The brewery s limits for which analytical values, we will accept for selected parameters. Production log Declaration The brewery s registrering of the used process for each batch, i.e. temperatures, times and volumes. The Brewery s garanti to the consumers and the authorities about certain contents in the final product. Does not explain the used recipe nor process. 5

5 Content of Sampling Plan Sample ID normally a number Sample type e.g. boiled wort Sampling point e.g. wort kettle Sample amount Frequency e.g. daily Responsible for sampling e.g. Production or Laboratory Sampling method e.g. microbiological sample Analysis verbal description, e.g. iodine test Analytical method e.g. EBC Analytica Responsible for the analysis e.g. Production or Laboratory Logging and reporting of results reference to QM-system Recipients the direct responsible for action/approval as well as copied 6

6 Example of Chemical Sampling Plan Process Area PRODUCT TYPE SAMPLE TYPE SAMPLE FREQUENCY ANALYSIS METHOD SAMPLE SIZE Fermentation Beer. Fermenting Extract Chemical Daily Saccharometer 750 ml Beer. Fermenting VDK or Diacetyl Chemical Each CCT, end of fermentation EBC ml Beer. Fermenting OE Alcohol ph Colour Bitterness Chemical Each CCT, start of cooling EBC 9.4 EBC EBC 9.35 EBC 9.6 EBC ml BBT Filtered beer OE (+RDF) alcohol ph Colour CO 2 Dissolved oxygen Haze Taste Chemical, sensory Each BBT EBC EBC EBC 9.35 EBC 9.6 EBC EBC 9.37 EBC ml 7

7 Example of Microbiological Sampling Plan Process Area PRODUCT TYPE SAMPLE TYPE SAMPLE FREQUENCY ANALYSIS METHOD SAMPLE SIZE Packaging, glass bottles Beer before filler Total counts (CFU) Beer spoilage organisms Microbiological Dynamic DEV Nähragar NBB bouillon Min. 8 l container Packaged beer flash pasteurised only Shelf life Total counts (CFU) Beer spoilage organisms Microbiological At start and each change Daily Weekly Check DEV Nähragar NBB bouillon Visual control of sample after storage 2 contain. 1 contain. 1 contain. Washed bottles for aseptic filling Total counts (CFU) Beer spoilage organisms Microbiological Weekly Dynamic DEV Nähragar NBB bouillon 8 contain. CIP Last rinse water Total counts (CFU) or Beer spoilage organisms Microbiological Weekly Dynamic DEV Nähr agar 100 ml 8

8 Example of the simplest production log: Brewhouse log 9

9 Example of a production log: Idealised fermentation chart Fermentation control: Temperature and % Plato during fermentation: Allows the Brewer to spot slow fermentations and when fermentation has ended. Cost and effort: Almost nothing % P Days fermented 10

10 More completed Fermentation Chart: Included some Carlsberg actions Operation Manual and some QC data Fermentation/Maturation Record Beer Brew No. CB 5-9 Tank Tank Vol. Aeration No. hl (A) DO ppm type gen 4 Yeast Pitching target pitching vol, cells, 10 6 /ml (B) % viabil. (C) hl (D) rate, 10 6 /ml (E) Pitching control pitched vol, hl (J) 25.0 cells, 10 6 /ml (F) 1560 Yeast Cropping vol, hl (G) 55 growth (H) = 0 C -- = %P -- = ph -- = cells Start cooling when Diacetyl is below 50ppb In case of 2 Tank fermentation: Transfer through plate heat exchanger to treatment tank Day Date 23-Jun 24-Jun 25-Jun 26-Jun 27-Jun 28-Jun 29-Jun 30-Jun 01-Jul 02-Jul 03-Jul 04-Jul 05-Jul 06-Jul 07-Jul 08-Jul 09-Jul 10-Jul 11-Jul 12-Jul 13-Jul 14-Jul 15-Jul 16-Jul 17-Jul 18-Jul 19-Jul 20-Jul 21-Jul 22-Jul Temperature, 0 C % Plato ph Operation Yeast Count, 10 6 /ml Diacetyl, ppb y c/d* c/d* d Day Zero is calculated from "Pitched volume". Yeast Count = (J x B x C) / (A x 100) Comments: d* = draining day choosen depending on yeast sedimentation behaviour (i.e. at 4 C usually typical). Must be continued periodically until Tank is emptied. 11

11 What to measure? Chemical parameters (Alcohol, Colour, Bitterness) Microbiology (level of infection, pitching yeast standard) Raw material checks (malt, hops, glass bottles) Flavour (positive flavors and off flavors) Packaging quality (fill level, labelling standards) Customer satisfaction (product shelf life) Manufacturing logs: Raw materials used Mashing diagrams Fermentation logs Samples taken / analysed 12

12 Example of Wort chemical Analysis - basic 13

13 Only 7 chemical parameters define our Basic Beer Style and - Quality 1) Original Extract OG [% P] g/100 g 2) Color EBC Units 3) Haze 20 o C EBC Units 4) Real Degree of Fermentation RDF [%] 5) ph [number] 6) Alcohol by Volume ABV [%] ml/100 ml 7) CO 2 [%] g/100 ml 15

14 These 7 chemical parameters can all be measured in the brewery by simple means 16

15 The brewer s choices 1. Basic instruments: Cheap 2. Basic instruments: Costs < Portable instruments: Costs >

16 Crushed Malt after the mill often forgotten Visual examination of crushed malt enables checks for: whole kernels (should not be there!) That all kernels are squeezed Some flour not too much 18

17 Brewhouse test: Iodine test Shows, whether the mash has converted 19 19

18 Extract analysis in spent grains: Shows whether we obtain the extract from the malt Example of a spent grains press Saccharometer 20

19 Refractometer: Shows OG of wort Accuracy: +/- 0.2 % P Obs.: Other limitations; but convenient Microbrewer using refractometer Refractometer, Lab. Bench type Refractometer, hand held 21

20 Saccharometer Shows OG of wort Accuracy: +/- 0.1 % P Saccharometer Selection of Saccharometers Reading af Saccharometer 22

21 Use of Saccharometer in practice as seen still in many breweries Time consuming very manual 23

22 Pycnometer Shows OG of wort Methods with highest accuracy: Densitometry using Pycnometry Accuracy: +/ % P 24

23 ph meter Used for control of mashing and wort boiling ph control Accuracy: +/- 0.1 ph Lab bench ph - meter Students measuring wort ph 25

24 More Brewhouse tests For check of efficiency of wort boil: For check of fermentation start: Break formation measured by an Imhoff cone Wort aeration checked by a simple flow meter 26

25 ...and some packaging tests, simple, yet useful some are rarely seen used Humidity test - labels Air in headspace test Glue test 27

26 CO2 measurement of bottled beer to ensure same pressure in each bottle Fast not so accurate 28

27 Taste testing: A Powerfull Quality and Diagnostic Tool to reveal process defects Taste test scores: 29

28 The brewers choices 1. Basic instruments: Cheap 2. Basic instruments: Costs < Portable instruments: Costs >

29 The microscope cheap, yet powerfull Microscope found in the brewmaster s Office next to his PC. Acetic Acic bacteria found In draft beer from a brewery 31

30 Microscope 32

31 FermentoFlash example of a fast way of obtaining a chemical beer analysis Alcohol (% v/v & w/w) Extract real & apparent Original gravity Density Osmotic pressure Accuracy: +/ % ABV Degassing of sample 10 ml sample Analysed in 1 minute Printer or PC Calibration with reference beer (up to 18 different) Prize: ~ VAT 33

32 The brewer s choices 1. Basic instruments: Cheap 2. Basic instruments: Costs < Portable instruments: Costs >

33 Portable Density Meter to achieve fast and accurate chemical parameters Density Specific gravity Extract Alcohol Viscosity mpa*s Temperature compensated 0 40 C Sample: 2 ml Time: Few seconds Price: Approx

34 Portable CO 2 / DO Measurement to measure Oxygen take up during production Several suppliers Anton Paar CarboQC Dr. Thiedig Digox Pentair s CO 2 Gehaltemeter 36

35 Lab. bench Methods accurate, not cheap High accuracy (do You need it?) Densitometry using automatic apparatuses (most common is PAAR densitometer based on ultrasonic vibrations. The PAAR unit may be a stand-alone or may be built into an automatic complete wort analyzer called a SCAWA) 37

36 Automised micro methods Beckman Coulter: Vi-Cell viabilitet by Trypan Blue staining in flow cell Aber instruments: Lab Yeast Analyzer: Viability by. capasitans metering Chemometec: NucleoCounter viabilitet by Iodine staining 38

37 Customer s satisfaction some times forgotten 1. Do You know, what your customers think of your beers in trade? 2. Winning beer competitions is nice, but your everyday customer pays your income 3. Do You systematically collect complaints statistics and use complaints for process Improvements? 39

38 Conclusion: Quality can be managed and product consistency achieved even on a small Budget and using limited efforts! Thank you for your attention! Axel G. Kristiansen

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