MUSSELING UP MATT MILLER NZ FATS AND OILS NOV 2016

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1 MUSSELING UP MATT MILLER NZ FATS AND OILS NOV 2016

2 RE-VALUING THE MUSSEL WITH HVN The aim to increase the value of Greenshell Mussel (GSM) based food export products. This will be achieved by determining the effect of these GSM products (and bioactives within) on improving joint and bone health while reducing inflammation Cawthron Institute and Sanford Limited business led research Through 4 objectives, we aim to develop the evidence dossier to make a general health claim for GSM food products We will deliver at least 4 new, high-value functional food products for export

3 GREENSHELL MUSSEL INDUSTRY EXPORTS 2015 Value (NZD$ - FOB) 2015 Volume (Kg) Price ($/KG) Greenshell Mussels $256.7m 27,877,370 ~$8-9 Mussel Oil $32.7m 10,958 >$3000 Freeze Dried Mussel Powder $12.1m 255,192 ~$50

4 SCIENCE PLAN Objective 1: To characterise known and novel active components of GSM using new high-throughput analytical capability. Objective 2: To demonstrate the efficacy of GSM foods/ingredients using in vivo and in vitro models of inflammation, joint and bone health.

5 SCIENCE PLAN Objective 3: To develop targeted consumer-focused functional formulated foods containing GSM ingredients and to develop an anti-inflammatory activity index to assess the efficacy for GSM food products. Objective 4: To determine the comparative nutrikinetics including the bioavailability of orally administered specific active ingredients from whole mussels, mussel extracts and novel functional foods in humans.

6 NIR NEAR INFRARED SPECTROSCOPY

7 COMPOSITIONAL ANALYSIS OF GSM Proximate analysis (i.e. fat, protein, glycogen, moisture) Provides insight into: physiological status or condition quality (sensory properties, consumer acceptance) Further lipid analysis: EPA, DHA, other fatty acids Applied to studies (to mussels) on: reproductive conditioning physiology and nutrition ecology genetics sensory analysis (taste, market acceptance)

8 PROXIMATE ANALYSIS (I.E. PROTEIN, FAT, GLYCOGEN, MOISTURE PLUS OILS) Standard (chemical) lab techniques: Food lab Well established methods Precise and reproducible However Costly ($500 per sample) Slow turnaround (eg. week) Rapid NIR analytical techniques: Fast (10 seconds), cheap, high throughput Method development ($$) Doesn t need precision of lab methods Can determine Fat, protein, glycogen, moisture, ash? EPA? DHA?

9 INTRODUCTION TO NIRS NIR absorption: associated with molecular vibration NIR (700 to 2500 nm) NIR absorption of cookie dough

10 MODEL DEVELOPMENT (CALIBRATION) Predicted Spectral data + Chemical data Absorbance Units Sample code % Fat % Protein % Carbs % Moisture A B C D etc..etc.etc.etc.etc Wavenumber cm Mussels_HVN-001_F_ Mussels_HVN-002_F_ Mussels_HVN-003_F_ Mussels_HVN-004_M_ Mussels_HVN-005_M_ Mussels_HVN-006_M_ Mussels_HVN-007_F_ Mussels_HVN-008_F_ Mussels_HVN-009_F_ Mussels_HVN-010_M_ Mussels_HVN-011_M_ Mussels_HVN-012_M_ Mussels_HVN-013_MIX_ Mussels_HVN-014_MIX_ Mussels_HVN-015_MIX_ Mussels_HVN-016_MIX_ Mussels_HVN-017_MIX_ Mussels_HVN-018_MIX_ Mussels_HVN-019_MIX_ Mussels_HVN-020_MIX_ Mussels_HVN-021_MIX_ Mussels_HVN-022_MIX_ Mussels_HVN-023_MIX_ Mussels_HVN-024_MIX_ Mussels_HVN-025_MIX_ Mussels_HVN-026_MIX_ Mussels_HVN-027_MIX_ Mussels_HVN-028_MIX_ Mussels_HVN-029_MIX_ Mussels_HVN-030_MIX_ Mussels_HVN-031_MIX_ Mussels_HVN-032_MIX_ Mussels_HVN-033_MIX_ Mussels_HVN-034_MIX_ Mussels_HVN-035_MIX_ Mussels_HVN-036_MIX_ Mussels_HVN-037_MIX_ Mussels_HVN-038_MIX_ Mussels_HVN-039_MIX_ Mussels_HVN-040_MIX_ Mussels_HVN-041_MIX_ Mussels_HVN-042_MIX_ Mussels_HVN-043_MIX_ Mussels_HVN-044_MIX_ Mussels_HVN-045_F_ Mussels_HVN-046_F_ Mussels_HVN-047_F_ Mussels_HVN-048_M_ Mussels_HVN-049_M_ Mussels_HVN-050_M_ Mussels_HVN-051_F_ Mussels_HVN-052_F_ Mussels_HVN-053_F_ Mussels_HVN-054_M_ Mussels_HVN-055_M_ Mussels_HVN-056_M_ Mussels_HVN-057_F_ Mussels_HVN-058_F_ Mussels_HVN-059_F_ Mussels_HVN-060_M_ Mussels_HVN-061_M_ Mussels_HVN-062_M_ Mussels_HVN-063_F_ Mussels_HVN-064_F_ Mussels_HVN-065_F_ Mussels_HVN-066_M_ Mussels_HVN-067_M_ Mussels_HVN-068_M_ Mussels_HVN-069_F_ Mussels_HVN-070_F_ Mussels_HVN-071_F_ Mussels_HVN-072_M_ C:\Users\Mattm\Desktop\Temp folder\calibration validation\raw Data Files\HVN Mussels_HVN-073_M_ Mussels_HVN-074_M_ Mussels_HVN-075_F_ Mussels_HVN-076_F_ Mussels_HVN-077_F_ Mussels_HVN-078_M_ Mussels_HVN-079_M_ Mussels_HVN-080_M_ Page 1/1 HVN-001;F;;HVN HVN-002;F;;HVN HVN-003;F;;HVN HVN-007;F;;HVN HVN-008;F;;HVN HVN-004;M;;HVN HVN-009;F;;HVN HVN-005;M;;HVN HVN-006;M;;HVN HVN-010;M;;HVN HVN-011;M;;HVN HVN-012;M;;HVN HVN-013;MIX;;HVN HVN-014;MIX;;HVN HVN-015;MIX;;HVN HVN-016;MIX;;HVN HVN-017;MIX;;HVN Mussels HVN-018;MIX;;HVN HVN-019;MIX;;HVN HVN-020;MIX;;HVN HVN-021;MIX;;HVN HVN-022;MIX;;HVN HVN-023;MIX;;HVN HVN-024;MIX;;HVN HVN-025;MIX;;HVN HVN-026;MIX;;HVN HVN-027;MIX;;HVN HVN-028;MIX;;HVN HVN-029;MIX;;HVN HVN-030;MIX;;HVN HVN-031;MIX;;HVN HVN-032;MIX;;HVN HVN-033;MIX;;HVN HVN-034;MIX;;HVN HVN-035;MIX;;HVN HVN-036;MIX;;HVN HVN-037;MIX;;HVN HVN-038;MIX;;HVN HVN-039;MIX;;HVN HVN-040;MIX;;HVN HVN-041;MIX;;HVN HVN-042;MIX;;HVN HVN-043;MIX;;HVN HVN-044;MIX;;HVN HNV-045;F;;HVN HVN-046;F;;HVN HVN-047;F;;HVN HVN-048;M;;HVN HVN-049;M;;HVN HVN-050;M;;HVN HVN-051;F;;HVN Mus 19/04/2016 HVN-052;F;;HVN HVN-053;F;;HVN HVN-054;M;;HVN HVN-055;M;;HVN HVN-056;M;;HVN 057;F;;HVN 058;F;;HVN 059;F;;HVN 060;M;;HVN 061;M;;HVN 062;M;;HVN 063;F;;HVN Mussels 18/05/ ;F;;HVN 065;F;;HVN 066;M;;HVN 067;M;;HVN 068;M;;HVN 069;F;;HVN 070;F;;HVN 071;F;;HVN 072;M;;HVN 073;M;;HVN 074;M;;HVN 075;F;;HVN Mussels 076;F;;HVN 077;F;;HVN 078;M;;HVN 079;M;;HVN Mussel HVN - 080;M;;HVN 16/06/ /07/2016 Optimising the model : - define scan region - mathematical processing - define # of factors - outlier removal Chemometric modelling Actual Develop model

11 80 GSM MUSSEL SAMPLE SPECTRA Wavenumber cm Mussels_HVN-001_F_ Mussels_HVN-002_F_ Mussels_HVN-003_F_ Mussels_HVN-004_M_ Mussels_HVN-005_M_ Mussels_HVN-006_M_ Mussels_HVN-007_F_ Mussels_HVN-008_F_ Mussels_HVN-009_F_ Mussels_HVN-010_M_ Mussels_HVN-011_M_ Mussels_HVN-012_M_ Mussels_HVN-013_MIX_ Mussels_HVN-014_MIX_ Mussels_HVN-015_MIX_ Mussels_HVN-016_MIX_ Mussels_HVN-017_MIX_ Mussels_HVN-018_MIX_ Mussels_HVN-019_MIX_ Mussels_HVN-020_MIX_ Mussels_HVN-021_MIX_ Mussels_HVN-022_MIX_ Mussels_HVN-023_MIX_ Mussels_HVN-024_MIX_ Mussels_HVN-025_MIX_ Mussels_HVN-026_MIX_ Mussels_HVN-027_MIX_ Mussels_HVN-028_MIX_ Mussels_HVN-029_MIX_ Mussels_HVN-030_MIX_ Mussels_HVN-031_MIX_ Mussels_HVN-032_MIX_ Mussels_HVN-033_MIX_ Mussels_HVN-034_MIX_ Mussels_HVN-035_MIX_ Mussels_HVN-036_MIX_ Mussels_HVN-037_MIX_ Mussels_HVN-038_MIX_ Mussels_HVN-039_MIX_ Mussels_HVN-040_MIX_ Mussels_HVN-041_MIX_ Mussels_HVN-042_MIX_ Mussels_HVN-043_MIX_ Mussels_HVN-044_MIX_ Mussels_HVN-045_F_ Mussels_HVN-046_F_ Mussels_HVN-047_F_ Mussels_HVN-048_M_ Mussels_HVN-049_M_ Mussels_HVN-050_M_ Mussels_HVN-051_F_ Mussels_HVN-052_F_ Mussels_HVN-053_F_ Mussels_HVN-054_M_ Mussels_HVN-055_M_ C:\Users\Mattm\Desktop\Temp folder\calibration validation\raw Data Files\HVN Mussels_HVN-056_M_ Mussels_HVN-057_F_ Mussels_HVN-058_F_ Mussels_HVN-059_F_ Mussels_HVN-060_M_ Mussels_HVN-061_M_ Mussels_HVN-062_M_ Mussels_HVN-063_F_ HVN-001;F;;HVN HVN-002;F;;HVN HVN-003;F;;HVN HVN-007;F;;HVN HVN-008;F;;HVN HVN-004;M;;HVN HVN-009;F;;HVN HVN-005;M;;HVN HVN-006;M;;HVN HVN-010;M;;HVN HVN-011;M;;HVN HVN-012;M;;HVN HVN-013;MIX;;HVN HVN-014;MIX;;HVN HVN-015;MIX;;HVN HVN-016;MIX;;HVN HVN-017;MIX;;HVN Mussels HVN-018;MIX;;HVN HVN-019;MIX;;HVN HVN-020;MIX;;HVN HVN-021;MIX;;HVN HVN-022;MIX;;HVN HVN-023;MIX;;HVN HVN-024;MIX;;HVN HVN-025;MIX;;HVN HVN-026;MIX;;HVN HVN-027;MIX;;HVN HVN-028;MIX;;HVN HVN-029;MIX;;HVN HVN-030;MIX;;HVN HVN-031;MIX;;HVN HVN-032;MIX;;HVN HVN-033;MIX;;HVN HVN-034;MIX;;HVN HVN-035;MIX;;HVN HVN-036;MIX;;HVN HVN-037;MIX;;HVN HVN-038;MIX;;HVN HVN-039;MIX;;HVN HVN-040;MIX;;HVN HVN-041;MIX;;HVN HVN-042;MIX;;HVN HVN-043;MIX;;HVN HVN-044;MIX;;HVN HNV-045;F;;HVN HVN-046;F;;HVN HVN-047;F;;HVN HVN-048;M;;HVN HVN-049;M;;HVN HVN-050;M;;HVN HVN-051;F;;HVN Mus 19/04/2016 HVN-052;F;;HVN HVN-053;F;;HVN HVN-054;M;;HVN HVN-055;M;;HVN HVN-056;M;;HVN 057;F;;HVN 058;F;;HVN 059;F;;HVN 060;M;;HVN 061;M;;HVN 062;M;;HVN HVN 063;F;;HVN Mussels Mussels 18/05/ /06/2016

12 104 GSM MUSSEL PROVISIONAL RESULTS

13 80 GSM MUSSEL PROVISIONAL RESULTS

14 g/100g RESULTS - MUSSEL COMPOSITION g/100g Female Site 1 Male Site 1 April May June July August September October April May June July August September October

15 mg/100g RESULTS - OMEGA 3 CONTENT mg/100g Female Site 1 Male Site 1 April May June July August September October April May June July August September October

16 MUSSELING UP PROJECT Still early days (8 months in to a 3 year program) In vitro and In vivo trials (Just starting) Clinical trial (starting late 2017) Novel food products for export markets

17 10 TH AAOCS BIANNUAL MEETING BIOTECHNOLOGY, LIPIDOMICS AND NUTRITION CHÂTEAU TANUNDA, BAROSSA VALLEY SA DATE TH SEPTEMBER 2017 FOR MORE INFORMATION CONTACT:

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