Cherry breeding at Dresden-Pillnitz Mirko Schuster Institute for Breeding Research on Horticultural and Fruit Crops

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1 Cherry breeding at Dresden-Pillnitz Mirko Schuster Institute for Breeding Research on Horticultural and Fruit Crops

2 Results - new cultivars Sour cherry Sweet cherry Achat Coralin Spinell Narana Jade Jachim Hamid Rubellit Boas Habunt PiSue192

3 Sour cherry Pillar growing type The pillar type is characterized by a natural distinctive upright growth habit with shorter internode length, narrow branch angles and a fruit set on short shoots (spurs). 1. Narrow branch angles 2. Short internode length 3. Fruit set on spurs

4 Cross populations inheritance pillar type Parents Seedlings Jachim self 210 Jachim o.p. 204 Jachim Oblacinska 84 Jachim B7,7,55 52 Vowi Jachim 22 Internode length: 'Jachim': 0,8 2,7 cm 'Boas': 0,6-2,3 cm 'Schattenmorelle': 1,1 2,1 cm 'Safir': 0,9 2,4 cm Greenhouse 15. May 2012

5 Resistance breeding Cherry leaf spot Blumeriella jaapii Blossom blight Monilinia laxa

6 Cherry leaf spot Sweet cherry New breeding material (2x) 'Namati' x clone 18,167 [P. avium x P. canescens] (susceptible) (resistant) F 2 progenies F 3 - crosses

7 Cherry leaf spot Sweet cherry F 2 cross population: 'Namati' (susceptible) x clone 18,167 (resistant) Resistant seedling (DK1,2,42) Susceptible seedling (DK1,2,16) Inheritance F 2 -population: resistant : susceptible 1 : 1 one dominante factor Cooperation with Michigan State University, East Lansing molecular marker for leaf spot resistance are developed

8 Cherry leaf spot Sour cherry New breeding material (4x)

9 Cherry leaf spot Sour cherry combination resistant susceptible total clone 3,105 x 'Fanal' , clone 3,106 x 'Fanal' , 'Schattenmorelle' x clone 3, , 'Schattenmorelle' x clone 3, , Inheritance of LS resistance F 2 seedling population (4x) resistant : susceptible 1 : 1 c 2 1 : 1 P % Hypersensitive reaction

10 Blossom blight (Monilia laxa) Sour cherry Rating scale (1 9) scale: 3 Jade 4 Achat 5 Köröser G. scale: 7 Ciganus 8 Mari Timpurii 9 F5,12,100

11 Blossom blight sour cherry cultivars Sorte Monilia 1-9 Krupnoplodnaja 1 Tschernokorka 1 Namati 1 P. avium 'Napoleon' 1 P. incisa 1 P. maackii 2 P. serrulata var. spontanea 2 P. incisa 'Incana' 2 P. dawyckensis 2 Wischnja 2,5 Jade 3 Schwäbische Weinweichel 3 Studenskaja 3 Naprumi 3 Sunburst 3 Vinka 3 P. avium 'Charger' 3 P. nipponica 3 Leitzkauer 3 Pandy 2 3 Tschernokorka 3 Timpurii de Pites. 3 Ferracida 3,5 Achat 4 Erdi nagy gymösteü 4 Eugenie imperatrice 4 Röhrigs Weichsel 4 Csengödi 4,5 Köröser Gierstädt 5 Ujfehertoi fürtös 5 Sorte = 9 cultivars Monilia 1-9 Morina 6 Pieraszt 6 Safir 6 Tarina 6 Fanal 6 Hartaer 6 Ludwigs Frühe 6 Leopolds Kirsche 6 Lara 6 Monilia Sorte 1-9 Ciganus 59 7 Debr. bötermö 7 Kantorjanosi 7 Karneol 7 Korai Pipacsmeggy 7 Korund 7 P. kurilensis 7 Mari Timpuri 8 Oblacinska 8 Pamjatch Vavilova 8 Spinell 8 Topas 8 Vowi 8 P. canenscens Pill 8 P. canescens EM 8 Hartei 8 Favorit 8 Korall 8 P. sargentii 'Klon 34' 8,5 Schirpotreb 8,5 Dropia 9 E1,3,33 9 F5, 12,100 9 F5, 13,122 9 Gerema 9 Kistiewa 9 Meteor korai 9 Pitic de Dragoslaven 9 Pitic de Jasi 9 Rubellit 9 Shukowskaja 9 Turgenjeva 9 Schattenmorelle 9 Haaks Dunkelsaftige 9 Nana 9 Paljus 9 Almas 9 = 17 cultivars = 33 cultivars Tolerant cultivars: Jade Schwäbische Weinw. Achat Ujfehertoi fürtös Morina Safir Fanal

12 Blossom blight (Monilia laxa) evaluation of seedling populations ( ) Resistant Monilia-blossom blight ( - ) Unclear (few symptoms) Monilia-blossom blight ( 0 ) Susceptible Monilia-blossom blight ( + )

13 Monilia-blossom blight evaluation of seedling populations ( ) Results of evaluation Monilia-bloosom blight Description: resistant susceptible tolerant? Combination number of trees Segregation total resistant susceptible tolerant r : a :? Schattenmorelle x Schwäbische Weinw : 1 : 1 Morina x Schattenmorelle : 1 : 1 Morina x Schwäbische W : 1 : 1 Morina x Stevnsbear Birgitte : 1 : 2 Morina x Stevnsbear Viki : 1 : 1 Jade x Stevnsbear Viki : 1 : 1 Ujfehertoi fürtös x Stevensbear 'Viki' : 1 : 1 Gerema x Schwäbische Weinw : 1 : 1

14 Fertility S-alleles studies Results PCR with 2 nd intron Primer bp K S 7 S 7 S 7 S 7 S 7 S 7 S 7 S 7 S 7 S 7 S 2 bp S 5? S 5? S 4 S 4 S 4 S 4 S 4 S 4 S 4 S 4 S 4 S 4 S A S A S 3 S S S 1 S 1 S 1 S 1 S 3 1 S 1 S 1 1 S 1 S 6 S 6 S 14 S 14 S K S Summary of 734 S-genotypes of sweet cherry In: Schuster, M., Incompatible (S-) genotypes of sweet cherry cultivars (Prunus avium L.). Scientia Horticulturae (2012 in press)

15 Antje Zakostelecky Christine Grafe Henryk Flachowsky Thank you for your attention

16

17 Breeding goals Fruit quality Fruit set Ripening time (early + late) Resistance to biotic and abiotic stress Self-fertility / Fertility Fruit cracking

18 Breeding material Sweet cherry: 3257 seedlings 173 breeding clones (1 st selektion) 11 breeding clones (2 nd selektion) Sour cherry: 3522 seedlings 54 breeding clones (1 st selektion) 3 breeding clones (2 nd selektion)

19 Fruit size Sweet cherry Cell number Cell size mean of 2x 3 countings Mesocarp size / cell number

20 Fruit size / cracking Cell number Cell number Krupnoplodnaja - high cell number (Mesocarp) Kordia Hudson - small cell number (Mesocarp) Regina Regina Hudson Kordia Krupnoplodnaja 28,9 mm 29,1 mm 29,2 mm 27,5 mm

21 Fruit size /cracking Cell size Cell size Krupnoplodnaja - small cell size (Mesocarp) Kordia Hudson - large cell size (Mesocarp) Regina Regina Hudson Kordia Krupnoplodnaja 28,9 mm 29,1 mm 29,2 mm 27,5 mm

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