Southeastern Grape Improvement and Distribution Program

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1 Southeastern Grape Improvement and Distribution Program PRESENTED BY PD Violeta Tsolova Florida Agricultural and Mechanical University Center for Viticulture and Small Fruit Research, College of Agriculture and Food Sciences Tallahassee, Florida.

2 Our Program The Southeastern NCPN Regional Clean Center for Grape is serving the viticulture region of Florida and neighboring Gulf States positioned in the PD s (Pierce s Disease) zone of the American Southeast. The mission of our Center is to provide high quality clonallypropagated grapevines regionally adapted muscadines, and PD s tolerant American natives hybrids free of targeted transmittable, diseases through testing, diagnosis, and therapy. Website: Plant(NCPN)CenterforGrape

3 Florida A&M University s Center for Viticulture and Small Fruit Research is maintaining for industry use the disease free G1/G2 nuclear planting stock of single copy origin grapevines and 4.5 acres Foundation Vineyard (G2) which currently is planted with 25 economically important muscadine and Florida native hybrid varieties. RESEARCH TEAM Dr. Violeta Tsolova- Professor, Viticulture and Developmental Biology, Program Leader: (850) ; violeta.tsolova@famu.edu Dr. Anthony. Ananga- Assistant Professor, Food Biotechnology: (850) ; anthony.ananga@famu.edu Dr. Islam El-Sharkawy - Assistant Professot, Breeding and Crop Production, (850) islam.elsharkawy@famu.edu Dr. James Obuya- Post Doctoral Research Associate, Plant Pathologist: (850) ; james.obuya@famu.edu Our Program

4 Program Partners and Beneficiary We work closely with the current programs of the NCPN, other academic institution in the region, local nurseries, Florida Viticulture Advisory Council, the Florida Wine and Grape Growers Association and the Florida Department of Agriculture through research, methods development, risk management, educational workshops and field days to advance the competitiveness and growth of the regional grape and wine industry and secure disease free planting stock for nurseries and individual growers.

5 Program Objectives Producing high quality disease free muscadine and Florida hybrid grape varieties by in vitro meristem culture. Conduct research and developed rapid methods for diagnostic of muscadine and PD tolerant American native grape varieties for crown gall, viruses and Eutypa dieback. Sustain the foundation block G1. Operate the Diagnostic Facility for American Native Southern Grapes Assist the extension and certification program.

6 Trends in FL Wine Industry Growth of Certified Florida Wineries % % % % % www. FWGGA.org www. tryfloridawines.com

7 Industry Demand Increase demand from nurseries in Florida, Georgia and Texas for licensing of our proprietary muscadine variety Majesty and inclusion of new muscadine varieties into the Southeastern Clean Vine Program due to an interest for exporting muscadine planting stock abroad (China and Brazil).

8 Majesty Patent US Muscadine grape plant named 'Majesty

9 Grant/Onyx FAMU Proprietary Selection

10 Floriana FAMU Proprietary Selection

11 On going projects: New varieties (6) and breeding lines (3) are undergoing disease elimination and to be included in the Clean Vine Foundation Block" by nursery request (partial NCPN funding FY 2015). A rapid highly specific early monitoring program for Eutypa dieback in muscadine grapes using molecular diagnostics ( in state funding FY 2015) Surveying GLRaV-1 in southern vineyards and in house diagnostic capability (in state funding FY 2016 ) To continue to evaluate the healthy status of the 1st generation vines in the Foundation vineyard in harmonization with the accepted guidelines of the Grape NCPN (1 st audit completed in 2015 with partial NCPN FY2015 funding). Two additional multi[purpose labs are under development in the newly designed "Viticulture Field/Head House Facilities Project: Postharvest Evaluation and Plant Pathology (Evans Allen Formula Grant Funding).

12 Deaback in Muscadine Grapes

13 Reasons are complex and poorly understood: OIV Review: International Council on Grapevine Trunk Diseases (ICGTD) April 2016 Several factors (Rubio and Garzón, 2011) could be involved in recent trunk diseases development: i) Increased number of the contaminated asymptomatic stocks in vineyards. ii) Infections brought to vineyards by infected planting material. Grapevine Trunk Diseases. iii) Cultural practices in vineyards. These usually focused to have grape yield during the first years and, giving a poor pruning wound protection.

14 Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines Magnolia variety with GLRaV-1 symptoms

15 Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines Observation and Scouting for Local Vectors

16 Leafroll Disease (GLRaV-1 ) in Muscadine Grapevines 2015 Audit of the Foundation Vineyard Viruses: GLRAV-1 muscadine samples only Grapevine leaf roll associated virus-1 RB Lenoir and Black Spanish cvs foundation material) Grapevine red blotch associated virus

17 Southeastern Vine Improvement and Distribution Program 25 varieties: 19 muscadines+2 rootstocks + 6 FL hybrid var. Disease Elimination: In vitro mm meristem culture Source Block Pathogen Testing: after 6-12 months growth BMP vineyard CV&SFR 21 viruses+eytypa, Agro, PD ( mother vines after 4 years quality observations Pathogen Testing: 18 viruses+eytypa, Agro, PD Foundation vineyard Disease Free G1 Nuclear Planting Stock -1 copy in screen house Southeast Grape Certification Program Certified Planting Stock for the Industry Certified Block in Registered Nurseries

18 In state support FL Ag Commissioner Adam Putnam touring our Center

19 In service of the Industry and Community Viticulture Center Helps Invigorate Muscadine Wine Industry FAMU Hosts Grape Harvest Festival Sweet Florida Native Helps FAMU Enhance its Brand FAMU's Center for Viticulture and Small Fruit Research... Enhancing and promoting research productivity - grantsmanship, publication, and participation in professional organizations.

20 At FAMU, Great Things Are Happening Every Day. established 1887

V. Colova(Tsolova) Center for Viticulture & Small Fruit research College of Engineering Sciences, Technology and Agriculture Florida A & M University

V. Colova(Tsolova) Center for Viticulture & Small Fruit research College of Engineering Sciences, Technology and Agriculture Florida A & M University National Clean Plant Network Program /Tier 2: GRAPE National Clean Plant Network FY2010 Southeastern Vine Improvement and Distribution Program: Disease Free G1 /G2 Planting Stock for Viticulture Industry

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