Little Things That Make A Big Difference: Yeast Selection. Yeast selection tasting

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1 Little Things That Make A Big Difference: Yeast Selection Yeast selection tasting

2 Wine Aroma PRIMARY AROMAS Grape-derived Monoterpenes (floral, fruity) Norisoprenoids (floral, perfumy) Methoxypyrazines (bell pepper, asparagus) Thiols (fruity aromas) Aliphatic phenylpropanoids Aging & Oak derived Furan derivatives (toasty and caramel) Lactones(coconut, woody, sweet, vanilla) TERTIARY AROMAS SECONDARY AROMAS Fermentation & yeast derived Esters and higher alcohols (fruity aromas) Volatile phenols (leather, barnyard, medicinal, spicy) Sulfur (asparagus, corn, molasses, cabbage, onion, rubber)

3 About the Project Evaluating the impact of different yeast selections on Sauv blanc wine style EC1118 Saccharomyces cerevisiae/bayanus, isolated in Champagne, high alcohol tolerance and ferment at low temp, strong competitive character Neutral character CY3079 Saccharomyces cerevisiae, isolated in Burgundy, selected to complement aromatic expression of typical white Burgundy styles Early yeast autolysis develop yeast derived characters such as toasted bread, honey, hazelnut and almond as well as roundness in mouth VL3 Zymaflore, isolated in Bordeaux, expresses varietal character in Sauv blanc Releases thiols from odorless precursors (boxwood, broom, citrus and passion fruit) QA23 Saccharomyces cerevisiae/bayanus, isolated in Portugal, thiol converter to develop varietal character in Sauv blanc Passion fruit

4 Fruit Processing Sauvignon blanc from Loasa Vineyard in Big Valley AVA Juice Data Analysis Date Tank/Variety/Blend Brix ph Unit TA (g/l) Malic Acid (mg/l) NOPA 9/21/ SB03SBYEAST missing 220 Direct to Press Yield gallons per ton equivalent ~35 minute press cycle 50 mg/l Sulfur Dioxide Cold settled overnight at 5 C Racked Split to 2 x 13 gallon reps Yeast = EC1118/CY3079/VL3/QA23 Ferment at 15 C until dry (~1 month)

5 Yeast selection wine fermentation curves 25 17SB03SBYEAST EC Brix 10 5 EC1118 A EC1118 B 0 9/22/ /2/ /12/ /22/ /1/ Time 25 17SB03YEASTLC CY Brix 10 5 CY3019 A CY3109 B Time

6 Yeast selection wine fermentation curves SB03SBYEAST VL3 15 Brix 10 5 VL3 A VL3 B 0 9/22/ /2/ /12/ /22/ /1/ /11/ Time 25 17SB03SBYEAST QA Brix 10 5 QA23 A QA23 B 0 9/22/ /2/ /12/ /22/ /1/ Time

7 Wine Data Wine Data Analysis Date Tank/Variety/Blend Alcohol (%) ph Unit TA (g/l) Residual Sugar (g/l) Malic Acid (mg/l) Acetic Acid (g/l) 4/5/ SB03YEASTLC EC1118-A /5/ SB03YEASTLC EC1118-B /5/ SB03YEASTLC QA23-A /5/ SB03YEASTLC QA23-B /5/ SB03YEASTLC CY3079-A /5/ SB03YEASTLC CY3079-B /5/ SB03YEASTLC VL3-A /5/ SB03YEASTLC VL3-B

8 Aroma profiles of different yeast selection Sauv blanc wines Biplot (axes F1 and F2: %) 5 hexanol trans-3-hexen-1-ol F2 (17.98 %) beta farnescene -10 CY3079_2 isobutyric acid beta-citronellol cis-3-hexen-1-ol CY3079_1 VL3_1 vanillin isobutanol acetoin beta-linalool VL3_2 geraniol hexyl acetate QA23_1 QA23_2 beta-demascenone ethyl isobutyrate trans-2-hexene-1-ol nerol oxide ethyl acetate hexanoic acid alpha-terpinene isoamyl alcohol ethyl butanoate ethyl diacetyl hexanoate beta-myrcene isoamyl acetate ethyl-3-methylbutyrate acetic octanoate acid ethyl lactate ethyl dihydrocinnamate 2-phenylethyl alcohol EC1118_2 1-octen-3-ol benzyl alcohol p-cymene ethyl decanoate phenethyl octanoic acid acetate nerolidol EC1118_1 camphor ethyl-2-methylbutyrate limonene F1 (54.25 %) Principal component analysis (PCA) biplot of volatiles Grape varietal aroma compounds Fermentation aromas QA23 Fermentation esters sweet fruity, banana, green apple CY3079 and VL3 Terpenes giving floral, fruity citrus aroma, green apple Alcohols and fatty acid more herbal green leafy aromas EC1118 Fermentation esters, higher alc, phenols low levels give floral, sweet fruity, waxy with some green undertones Active variables Active observations

9 Thank You to Our Event Partners

10 Thank You to Our Sponsors Ag Unlimited Encore Glass Novavine Scott Laboratories Umpqua Bank

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