Growing vines in sites infested with Xiphinema index

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UCCE Sonoma County Grape Day Growing vines in sites infested with Xiphinema index UCCE Sonoma County Grape Day Rhonda Smith UCCE Viticulture Farm Advisor Sonoma County Plant parasitic nematodes Non segmented, microscopic roundworms X. index: Vector of grapevine fanleaf virus, the causal agent of grapevine fanleaf disease Xiphinema index Virus is bound to esophagus lining in X. index Virus particles are lost at molt, do not pass through egg stage do not replicate inside nematode Image: courtesy of H. Ferris 1

Sauvignon blanc with fanleaf degeneration Abnormal leaf shapes ( fanleaf deformation ) Small internodes resulting in shorter shoots and bushy appearance Three leaf symptoms 1. Vein banding 2. Fan shaped leaf blades Photos: A. Walker 2

UCCE Sonoma County Grape Day 3. Yellow mosaic leaf symptoms in the spring Yellow mosaic 3

Reduced fruit set General guidelines: Sampling for nematodes in an established vineyard Collect soil samples that contain feeder roots In the vine row; 12 18 from the trunk Sample depth is at least 18. Remove the top 3. Divide the vineyard block into zones that have similar vine growth or soil texture. Within each zone, collect soil + roots from each of 5 randomly selected vines; a composite sample should be ~1 Qt (1 liter) Use a separate plastic bag for each composite sample Label each bag; call the lab for specifics 4

UCCE Sonoma County Grape Day Sample timing and challenges Mid October to mid February (highest populations) Moist soil; ideally after soil has drained Large variability in population density exists within a vineyard block at any time during the year. Nematodes can aggregate and vary between two adjacent vines. UC ANR Publication 3343, Grape Pest Management, 3 rd edition Once a vineyard has fanleaf degeneration, it will always be diseased and crop will be reduced each year When diseased vines are pulled, remaining roots provide a reservoir for the virus Complete decay of remnant roots is thought to take many years and X. index can survive on those roots When the site is replanted, the new vines will become infected and eventually show disease symptoms 5

Sonoma County vineyard fumigation permits Records search for Telone use permits in past six years (January 1, 2012 to February 2, 2018) Average of 8 applications of Telone per year Average treatment area is 11 acres per application. No Methyl Bromide applications have occurred for at least 10 years. Source: Sonoma County Agricultural Commissioner GRN Rootstocks Root lesion Nematode Citrus Nematode Ring Nematode Phylloxera Nodosities GRN-1 MR R R HR GRN-2 MR MS MS HR GRN-3 MR MR MR R GRN-4 MR MR MR R GRN-5 MR MR R MS All GRN rootstocks are resistant to Xiphenema index and 3 strains of root-knot nematodes Ferris, Zheng and Walker. 2012. Journal of Nematology 4(4) 6

UCCE Sonoma County Grape Day GRN Rootstock Parentage GRN1 GRN2 V. rupestris x V. rotundifolia Cowart (V. rufotomentosa x (Dog Ridge x Riparia Gloire)) x Riparia Gloire GRN3 (V. rufotomentosa x (Dog Ridge x Riparia Gloire)) x V. champinii c9038 GRN4 (V. rufotomentosa x (Dog Ridge x Riparia Gloire)) x V. champinii c9038 GRN5 (Ramsey x Riparia Gloire) x V. champinii c9021 Rootstock Trial in Cabernet Sauvignon Site: South end of Geyserville Planted: 2012 Soil: Yolo loam Spacing: 10 ft x 7 ft Trellis: Modified vertical shoot positioned Training: Bi-lateral cordon trained, spur pruned Design: Randomized complete block, 8 replications of 11 rootstocks using 5-vine plots 7

Rootstock Trial, Alexander Valley, 2017 Yolo sandy loam, overwash 1.5 to 1.7 inches available water per foot Rootstock Trial, Alexander Valley, 2017 Trial design: 8 replications of 11 rootstocks Replication 8 Replication 1 8

UCCE Sonoma County Grape Day GRN rootstocks can become infected with grapevine fanleaf disease. The question: Will they show disease symptoms? 9

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UCCE Sonoma County Grape Day 11

Thank you 12