J. Dairying, Foods & H.S., 29 (2) : 92-96, 2010 AGRICULTURAL RESEARCH COMMUNICATION CENTRE www.arccjournals.com / indianjournals.com STUDIES ON PREPARATION OF FLAVOURED MILK FROM COW MILK BLENDED WITH SAFFLOWER MILK K.C. Repate, V.J. Kamble, Hassan Bin Awaz and B.M. Thombre* Department of Animal Husbandry and Dairy Science, Marathwada Agricultural University, Parbhani 431 402, India ABSTRACT The preparation of flavoured milk from different proportion of cow milk blended milk safflower milk 100:0 ( ), 80:20 ( ), 70:30 ( ), 60:40 ( ) and 50:50 (T 5 ) was prepared and studied the acceptability. On an average the flavoured milk of treatment and contained fat 3.0 per cent, protein 3.20, 3.08, 2.91, 2.78 and 2.65 per cent, ash 0.72, 0.68, 0.66, 0.62 and 0.59 per cent total solids 15.21, 14.59, 14.03, 13.58 and 13.10 per cent, respectively. The sensory score overall acceptability of flavoured milk of treatment were 8.94, 8.66, 8.01, 7.30 and 6.37. The overall accetability score of has accetability close to control ( ). The overall acceptability score of and were also rated between like moderately to like very much. The cost of production of one litre flavoured milk of treatment was Rs. 12.63, Rs.11.63, Rs.10.64 and 9.65. Thus this proved that the cost of flavoured milk could be minimized by using safflower milk and cow milk blended and blending could be done to the maximum proportion of 50:50. Key words : Flavoured milk, Safflower milk, Cow milk. INTRODUCTION Milk production of India has risen gradually from a level of 17.0 million tonnes in 1950-51 to a level of 90.7 million tonnes in the year 2004-05. As per scientific recommendations of ICMR 280 g of milk per day per capita for consumption, however per capita availability of milk in India is only 232 g during the year 2004-05 (Tripathy, 2006). India ranks first in the milk production which account for about 10 per cent of the worlds milk production. The milk production in India is on a small scale and scattered, more over milk is perishable article and requires being disposed of immediately, either fluid or for preparation of products or for both. Half of the milk produced in the country is utilized at fluid milk and rest is concerted into tradional milk products (Rao and Raju 2003). From these some part of milk is used for making special milk like flavoured milk, soft curd milk, fermented milk standardized milk, and recombined milk are humanized milk. Flavoured milk a beverage in which sugar flavouring agents colouring material are added milk it contains all the constituents of milk. It is good *Corresponding author source of proteins, cabrohydrates and mineral. Provides energy and water to digest the food, regulate body temperature and prevent dehydration. Vegetative proteins from non legumes, particularly safflower occupied a unique and important position in the diet because they form a major and cheap source of vegetable proteins, fat, minerals and calories. Safflower milk does not contain, chloesterol and is rich in polysaturated fatty acids and have been shown to prevent an increase in serum cholesterol on a high fat diet and thus considered anti antherogenic. Safflower milk seems to be useful for lactose intolerant people and infants allergic to cow and buffalo milk. The cost of production of safflower milk is very less as compared to cow and buffalo milk. So looking to the improtance of safflower milk, efforts have been made to prepare flavoured milk from cow milk blended with safflower milk. MATERIAL AND METHODS Safflower seeds were used for preparation of safflower milk 50 gm dehusked safflower seeds was weighed and warned with hot water and then
it was ground with addition of water and filtered through muslin cloth (Seed : water ratio 1:10). So as to have consistency as that of milk. To improve heat stability sodium hexameta phosphate @ 0.2 per cent, common salt @ 0.05 per cent and sugar @ 0.2 per cent were added to enhance its taste and acceptability. The milk as then boiled. The milk so obtained had creamy colour nutty flavour, Sweet taste and consistency as that of cow milk Fig 1. Flow diagram for preparation of safflower milk dehusked safflower seeds (50 gm) Seeds washed with hot water Grinding of seed (in small amount of water) Filtration (Final seed to water ratio 1:10) Addition of sodium hexameta - phosphate (@ 0.2 per cent) Addition of common salt (@ 0.05 per cent) Heating of milk Safflower milk (500 ml) Standardization of safflower milk and cow milk : The safflower milk thus prepared as shown in Fig. 1 was adjusted the fat level of 3.0 per cent in the milk by adding safflower seed extract. The cow milk was standardized to 3.0 per cent fat and 9.0 per cent SNF. Standardization was done by adding skim milk and skim milk powder. Preparation of flavoured milk : For preparation of flavoured milk the procedure given by De (1982) was followed with slight modification. The measured quantity of cow milk/ safflower milk was taken. The milk was filtered through muslin cloth. The cow/ safflower milk was standardized to 3.0 per cent fat and 9.0 per cent SNF. Milk was heated to 75 O C. Then it was allow to cool upto 5 O C after cooling, Vol. 29, No. 2, 2010 93 colour and flavours were added in milk. Milk was filled in bottle and capping milk was stored in refrigerator. Fig.2 : Flow diagram fro preparation of flavoured milk Receiving milk (Cow milk/blended milk) Filtration /clarification Standardization (3.0 per cent fat and 9.0 per cent SNF) Addition of sugar @ 8.0 per cent sugar Heating of milk (Upto 78 O C) Cooling to 50 O C Addition of colour and flavour Bottle filling Capping Storage in refirigrator Flavoured milk Chemical analysis: The samples from various treatment combiantions were chemically analyzed for fat (IS; 1224, Part-I, 1997), Protein (A.O.A.C. 1965), total solids (IS: 1479, Part II, 1961). The carbohydrate was estimated by subtraction method i.e. Carbohydrate = Total solids - (Fat + Protein + Ash). Sensory evaluation: The flavoured milk prepared under different treatment combinations were suspected to sensory evaluation by a panel of judges for colour and appearance, flavour consistency and mouth feel. The scoring was done on 9 point hedonic scale as described by (Gupta 1976). Statistical analysis: The data contained were analyzed statistically by using completely randomized design as per method prescribed by Panse and Sukhatme (1985).
94 J. DAIRYING, FOODS & H.S. Cost of production of flavoured milk: The various constituents required for manufacture of falvoured milk was rated as per prevailing market price and cost per litre of flavoured milk was worked out. RESULTS AND DISCUSSION Milk used for preparation of flavoured milk Flavoured milk was prepared with cow milk blended with safflower milk. The flavoured milk prepared from cow milk served as control. The chemical composition of cow milk and safflower milk used for preparation of falvoured milk is given in Table 1. It can be seen from table that protein, carbohydrate, ash and total solids content of cow milk was significantly higher than safflower milk. Chemical composition: The chemical composition of flavoured milk is presented in Table 2. From the table 2 it is observed that the fat content of flavoured milk in all the treatment combination was same. As the cow milk and safflower milk used for preparation of flavoured milk was decreased from 3.20 ( ) to 2.65 ( ). It might be due to decreasing level of cow milk which contained more protein than safflower milk. Rajesh Kumar et. al. (1989) observed protein content of falvoured milk like beverage as 3-13 per cent. The carbohydrate content of was lowest (6.85) and that of was highest (8.29). As the proportion of safflower milk in the blended milk increased there was decreases in the carbohydrate content of flavoured milk. Dhanwade (2000) observed same trend for kalakand prepared from safflower milk blended with buffalo milk. The ash content was also found to be decreased from 0.72 ( ) to 0.59 ( ). This decreasing trend of ash content was due to increased proportion of safflower milk in the blend. The results recorded are comparable with Choi and Kosikowski (1985) who reported the ash content of sweetened flavoured carbonated yoghurt as 0.5 per cent. The total solids content in was highest (15.21%) and lowest in (13.10). There was decrease in total solids content in flavoured milk with increased level of safflower milk. There were significant differences in total solids content of flavoured milk. There results were in agreement with the findings of Rajesh Kumar et al.(1989) who reported total solids content of flavoured milk like beverage as 15.13 per cent. Sensory evaluation : It was observed from Table 3 that treatment which is control scored the highest score followed by (6.65). This indicated that increase in proportion of safflower milk in the blend, decreased the score of colour and appearance of flavoured milk. There results were in agreement with the findings of Narwade (1999) and Dhanwade (2000). The score in respect of flavour ranged between 6.41 to 8.94 for and treatment combinations. Significant differences were observed in the flavour score. In case of consistency the score recorded seem to be highest for (8.88) and lowest in as Table 1. Average chemical composition of cow milk and safflower milk used for preparation of flavoured milk Sr.No. Type of milk Fat (%) Protein (%) Carbohy-drate (%) Ash (%) Total solids (%) 1. Cow milk 3.00 3.38 4.90 0.72 12.00 2. Safflower milk 3.00 2.18 2.08 0.56 7.82 Table 2 : Chemical composition of flavoured milk Treatments Constituents (Per cent) Fat Protein Carbohydrate Ash Total Solids 3.00 3.20 8.29 0.72 15.21 3.00 3.08 7.95 0.68 14.59 3.00 2.91 7.45 0.66 14.03 3.00 2.78 7.17 0.62 13.58 3.00 2.65 6.85 0.59 13.10 S.E. + 0.00 0.032 0.053 0.0074 0.068 CD at5 % 0.00 0.096 0.15 0.021 0.20
Vol. 29, No. 2, 2010 95 Table 3 : Sensory score of flavoured milk Treatments Sensory score Colour and appearance Flaovur Consistency Mouthfeel Overall acceptability 9.00 8.94 8.88 8.98 8.94 8.91 8.46 8.85 8.73 8.66 8.30 7.80 7.78 8.21 8.01 7.26 7.16 7.05 7.76 7.30 6.65 6.41 6.15 6.31 6.37 S.E. + 0.095 0.077 0.087 0.093 0.053 CD at5 % 0.028 0.22 0.025 0.27 0.15 Particulars of input Table 4 : Cost structure of flavoured milk Items used under Cow milk (ml) 100 ml (Rs.12.00) 800 ml (9.60) 700 ml (8.40) 600 ml (7.20) 500 ml (6.00) Safflower milk (ml) 200 ml (0.41) 200 ml (0.41) 300 ml (0.61) 400ml (0.82) 500 ml (1.03) Sugar (g) 80(1.20) 80(1.20) 80(1.20) 80(1.20) 80(1.20) Colour and flavour 0.5 ml (0.42) 0.5 ml (0.42) 0.5 ml (0.42) 0.5 ml (0.42) 0.5 ml (0.42) Miscellaneous 1.00 1.00 1.00 1.00 1.00 Cost per kg of flavoured milk 14.62 12.63 11.63 10.64 9.65 Figures in parenthesis are cost (Rs.) of items. 6.15. The significant differences were found amongst the treatment combinations. Incase of mouth feel the mean score ranged from 6.31 to 8.98. It was lowest in and highest in. There were significant differences in the mouth feel score of treatment. The results obtained were in agreement with the findings of Narwade (1999). The overall acceptability score for treatment were 8.94, 8.66, 8.01, 7.30 and 6.37 respectively. Treatment was significantly superior over rest of the treatments. The flavoured milk of treatment and were rated in between like very much and like extremely. Flavour milk of were rated in between like moderately and like very much, while the flavoured milk of was rated in between like slightly and like moderately. The results obtained were in agreement with the results documented by Dhanwade (2000). Cost : The figures in Table 4 revealed that the cost of product so workout was 14.62 for pure flavoured milk ( ) where as cost of (flavoured milk blended with safflower milk) treatment combination. was Rs. 12.63, 11.63, 10.64 and 9.5 respectively. The cost of flavoured milk of in comparatively less than rest of the treatments. This milk was liked by the consumers. CONCLUSION It may be conducted that low cost flavoured milk could be preferred by using safflower milk blended with cow milk with addition of 8 per cent sugar and rose flavour. The cost of production of flavoured milk of was Rs. 9.65 only. REFERENCE A.O.A.C. (1965). Official Method of Analysis 11th Edn. Assoc. Official Chemists, Washington; 4 DC. USA. Choi, H.S. and Kosikowski, F.V. (1985). Sweetened plain flavoured carbonated yoghurt beverages. J. Dairy Sci., 68(3):613-619. De, S. (1982). Outlines of Diary Technology Oxford Univ. Press, Bombay, Calcultta, Madras pp: 98-99. Dhanwade, S.S. (2000). Studies on preparation of Kalakand from safflower milk blended with buffalo milk. M.Sc. (Agri.) Thesis M.A.U., Parbhani. Gupta, S.K. (1976). Sensory evaluation in food industry. Indian Dairyman, 28(7):: 293-295. ISI (1961). IS 1479 Determination of total solids Part - II.
96 J. DAIRYING, FOODS & H.S. ISI (1981). Indian Standard Institute. Hand Book for Food Analysis Part XI, Dairy Products, SP. 18 (Part XI). Narwade, S.G. (1999). Studies on preparation of kheer from safflower milk blended with buffalo milk. M.Sc. (Agri.) Thesis, M.A.U., Parbhani Panse, V.G. and Sukhatme, P.V. (1967). Statistical Methods for Agricultural Workers, 2nd Edn, ICAR, New Delhi. Rajeshkumar, Patil, G.R. and Rajor, R.B. (1989). Development of formulation of flavoured milk like beverages from Cheese whey. Asian J. Dairy Res., 6(3):121-124.