Copyright Advanced Viticulture, Inc. Mark Greenspan, Ph.D., CPAg, CCA Advanced Viticulture, Inc.

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Mark Greenspan, Ph.D., CPAg, CCA Advanced Viticulture, Inc. www.advancedvit.com

Irrigation Management Water Management Floor Management Weather Frost, Cooling, other. Strategy

Physiology of vine water stress Water management practices and strategies Irrigation minimization Wine style manipulation Irrigation management Scheduling Efficient water use Measurement & monitoring techniques Copyright Advanced Viticulture, Inc. www.advancedvit.com

Water is held within soil matrix by adhesive properties of water More water thicker film and water available from larger pores As soil dries, water film thins out and water nearer to surfaces requires more energy for extraction Ψ m -0.1 bar to -0.3 barskpa) (-30 cbars: Field Capacity Ψ m -3 bars (-300 cbars, kpa): mid-range, slightmoderate stress Ψ m -15 bars (-1500 cbars, kpa): Permanent Wilting Copyright Advanced Viticulture, Inc. www.advancedvit.com

Water flow in vine primarily via transpiration Evaporation of water in sub-stomatal cavity Water is pulled through the vine by cohesion of water molecules Plant water stress is from either or both: Drying soil, so lower (more negative) Ψ m Higher evaporative demand (VPD) As transpiration exceeds uptake: Cell turgor declines Cell volume declines Solute concentration within cells increases Ψ π of cell decreases (more negative, higher magnitude) Moderate water stress: Ψ leaf = -12 to -15 bars Severe water stress: Ψ leaf < -15 bars Copyright Advanced Viticulture, Inc. www.advancedvit.com

Stomata close to prevent water loss and prevent xylem cavitation Xylem cavitation (embolism) is a common occurrence in grapevine Not an adaptation Copyright Advanced Viticulture, Inc. www.advancedvit.com

Abscisicacid (ABA) and cytokininsare primary hormones affected by water stress ABA production stimulated rapidlyin response to water stress Cytokininproduction declines during water stress, but slowly Root ABA may be delayed in getting to leaves, but hydraulic signal (declining turgor) induces rapid production of ABA in the leaves ABA: stomatalclosure; reduces auxineffects in stimulating vegetative growth; effect on ripening Cytokinin: cell division; leaf abscission Copyright Advanced Viticulture, Inc. www.advancedvit.com

Plan to manipulate vine water status during the growing season Strategies may be imposed to change wine style in any given vineyard Strategies may be imposed to make efficient use of limited water resources Red varieties are more amenable to water deficit strategies than are white varieties Copyright Advanced Viticulture, Inc. www.advancedvit.com

Budbreak Control shoot growth Avoid stress during fruit set Avoid excess shoot growth Affect vegetative character of wines Bloom & Fruit Set Veraison Maturity Control berry size Skin:Pulp ratio Stimulate ripening enzymes Avoid heat stress Affect tannin and structure of red wines Maintain and stimulate ripening process Avoid heat stress Avoid berry shrivel Affect ripe fruit and jammy wine characteristics Copyright Advanced Viticulture, Inc. www.advancedvit.com

Vegetative growth very sensitive to water availability Do not provide too much water to over-stimulate shoots Do not over-stress vines. May cause poor fruit set Too much stress (LWP < -11 bars) Too little stress (LWP > -8 bars) * poor to very poor fruit set * insufficient canopy growth * excessive vegetative growth * vegetative flavors in wine * elevated disease risk * additional farming costs Copyright Advanced Viticulture, Inc. www.advancedvit.com

In wet winter climates, the soil profile is full at budbreak Prolong the uptake of water in the soil by delaying irrigation for as long as possible Avoid luxurious growth of canopy by appropriate cover crop management In a wet winter climate, biggest mistake is to irrigate too early with drip irrigation!!! Copyright Advanced Viticulture, Inc. www.advancedvit.com

Growing Slowing Stopped Dead Tip

Shoot growth most sensitive process to water stress

Delayed Irrigation Early Irrigation

Deficit irrigation: applying less water via irrigation than is being transpired, this reducing soil moisture reserves Control shoot growth before veraison Root growth less sensitive than shoot growth to water deficits Control berry size between set and veraison Stimulate ripening processes at and after veraison** ABA stimulates enzymes involved with ripening processes Elevated ABA is desired at veraisonin red varieties Maintain moderate stress during ripening without excessive stress Copyright Advanced Viticulture, Inc. www.advancedvit.com

Shoot Tip Stem Xylem Flow Phloem Flow Transpiration Width of line indicates relative amount of flow Berry Leaf

Shoot Tip Stem Xylem Flow Phloem Flow Transpiration Width of line indicates relative amount of flow Berry Leaf

Water relations and water status Berry size less sensitive to short-term water deficits; hence less yield impact Severe water stress can: retard sugar production and accumulation reduce canopy effectiveness; possible defoliation trigger premature berry desiccation Insufficient water stress can: dilute phloem sap and retard sugar accumulation reduce effect of veraison stress possibly induce vegetative growth Copyright Advanced Viticulture, Inc. www.advancedvit.com

Whites benefit less from water deficit than reds Three berry growth periods: Fruit set-near veraison / near-veraison / veraison to harvest Set to veraison deficit: Smaller berries good for tight-clustered cultivars More intense tannin in wines, but can give too much astringency Reduction in vegetative character in large canopies Magic window about 2 weeks before veraison Veraison to harvest deficit: Enhancement / acceleration of ripe characteristics Prevention of canopy growth and slow sugar accumulation Copyright Advanced Viticulture, Inc. www.advancedvit.com

Determine Site Characteristics Rooting Depth Soil Type Install Soil Moisture Sensors to: Determine ideal volume Determine nominal interval Monitor Continuous soil moisture profile Plant water status Check against target levels Soil Moisture Plant water status Copyright Advanced Viticulture, Inc. www.advancedvit.com Interval Adjust Volume

Volume and Interval Need to learn about your soils and your root systems Light Textured and/or gravelly Heavy Textured Shallow (< 24 ) Deep (>36 ) Very Low Vol. & Very Frequent Low Vol. & Frequent Moderate Vol. & Frequent High Volume & Infrequent Copyright Advanced Viticulture, Inc. www.advancedvit.com Hillsides Valley Floor

How much and how often? Evapotranspiration (ET) versus Soil moisture based scheduling

Greenspan, M.D. Integrated Irrigation Management in California. Practical Winery and Vineyard. Part 1: March/April 2005 Part 2: May/June 2005 Irrigation (inches) = ETo (inches) * Kc * Km Where: ETo is reference ET Kc is crop coefficient Km is management coefficient (% full ET) Copyright Advanced Viticulture, Inc. www.advancedvit.com

Uses soil-moisture sensors to determine depth of irrigation and depletion of soil moisture to determine intervals. Copyright Advanced Viticulture, Inc. www.advancedvit.com

Measure at multiple levels to determine depth of irrigation Measure continuously (electronic datalogger)! For drip irrigation, measure close to the dripper (4in., 10cm) Use to determine nominal irrigation volume per application Use to determine nominal irrigation interval between applications Use in conjunction with plant moisture measurements to adjust intervals Copyright Advanced Viticulture, Inc. www.advancedvit.com

emitter 18 in 9 in 0

Soil matric potential sensors Soil water content sensors & probes

Volumetric Matric Potential Advantages Available in multi-level Easy to interpret patterns Getting less expensive True physical measurement Does not require calibration Inexpensive* * Cost is increased with multiple sensors Disadvantages Very difficult to calibrate Therefore, not a physical measurement Discrete sensors not available in multi-level Measuresonly the wetter portion of SM Copyright Advanced Viticulture, Inc. www.advancedvit.com

Portable Fixed Advantages One instrument measures many locations Therefore, lower cost Neutron probe accuracy Continuous measurement Patterns of wetting and depletion can be very useful May be delivered by telemetry Disadvantages Snapshots only More expensivedue to multiple instruments Copyright Advanced Viticulture, Inc. www.advancedvit.com

Set a reference volume for refill point Upper limit of RAW Lower limit of RAW Progressive stress reflected in slope

Pressure Chamber Porometer Petiole Diameter

Pressure chamber Measures suction in the xylem vessels Advantages: Portability; repeatability; ruggedness Disadvantages: No indication of vine physiological response; reading depends on time since last irrigation, grape variety, daytime conditions, etc. Reading can be misleading on its own IMPORTANT: Place leaves in plastic bags immediately before removal and during measurement Copyright Advanced Viticulture, Inc. www.advancedvit.com

Porometer Measures stomatal conductance (facility of pores to transpire water) Advantages: Portability; vine response to water status; indirect indication of photosynthesis Disadvantages: Less rugged instrument; sensitive to environmental conditions; more variability than leaf water potential measurements Copyright Advanced Viticulture, Inc. www.advancedvit.com

What are stomata?

Suzy Y. Rogiers*, Dennis H. Greer, Ron J. Hutton and Joe J. Landsberg Journal of Experimental Botany, Vol. 60, No. 13, pp. 3751 3763, 2009 Copyright Advanced Viticulture, Inc. www.advancedvit.com

500 Units are in mmol/m 2 /s 450 400 350 300 250 200 150 100 50 0 Luxury Mid-Range Slight Mod. Stress Stress Target Range for White varieties High Stress Target Range for Red varieties Extreme Stress Copyright Advanced Viticulture, Inc. www.advancedvit.com

Romero et al., 2010 AJEV 61:300-312

Mark Greenspan, Ph.D., CPAg, CCA Advanced Viticulture, Inc. (707) 838-3805 www.advancedvit.com mark@advancedvit.com Water management Nutrition management General viticulture Vineyard design Vineyard management Vineyard development Copyright Advanced Viticulture, Inc. www.advancedvit.com