Recent Canola Meal Research with Broilers ( )

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Recent Canola Meal Research with Broilers (2010-11) Eduardo Beltranena and Matt Oryschak Research and Innovation Division Alberta Agriculture and Rural Development Edmonton, AB

Recent AARD broiler research involving CM 1. Nutrient digestibility in conventional, expeller-pressed and extruder-pressed CM 2. Nutrient digestibility in 2 air-classified CM fractions vs. the parent stock CM 3. Nutrient digestibility in B. napus vs. B. juncea meals 4. Performance of broilers fed graded inclusions of B. napus and B. juncea over d 0 to 35

Digestibility Studies

Our approach Basal diet Assay diet Wheat Veg oil Premix Cr 2 O 3 = Test Ingred Basal diet 30% 70% D test D assay D RC basal test D x = digestibility RC x = relative contribution RC basal

Our approach (cont d) Commercial starter diet Test diets d0 d14 d21

Measurements Feed disappearance measured over the experimental period Body weight on d 14 and d 21 Lab analysis of diets, ingredients, digesta and excreta

Nutrient Digestibility in Canola Meal for Broilers: Effect of oil extraction process

Alternative extraction processes (small scale, low capital)

Table 1. Analyzed nutrient content of canola meal types compared in the present study. Nutrient EXP CM EPCM SECM Crude Protein 29.54 35.60 37.92 Total Amino Acids 23.93 32.39 34.57 Crude Fat 17.12 12.75 4.76 Crude Fiber 10.04 6.01 7.24 ADF 22.34 16.08 15.49 NDF 27.79 20.17 24.39 Lysine 1.20 2.11 2.15 Methionine 0.54 0.69 0.74 TSAA 1.24 1.55 1.63 Threonine 1.16 1.52 1.58

Table 2. ATTD of GE and AID of CP and AA in samples of conventional, expeller-pressed and extruder-pressed canola meals. CM type P-value SECM EPCM EXCM SEM CM type Gross energy 48.44 c 56.73 b 67.77 a 1.88 <.0001 Crude protein 71.88 b 68.73 b 79.40 a 1.17 0.0002 Total AA 81.66 a 75.22 b 84.83 a 1.16 0.0005 Lysine 81.79 b 72.52 c 97.33 a 1.23 <.0001 Methionine 88.58 a 81.43 b 91.37 a 0.89 <.0001 Total Sulfur AA 82.20 a 75.79 b 84.92 a 1.17 0.0007 Threonine 73.88 b 67.44 c 85.80 a 1.31 <.0001 Tryptophan 78.86 c 85.08 b 88.86 a 0.99 <.0001

Table 3. AME and ileal digestible CP and AA content in samples of conventional, expeller-pressed and extruder-pressed canola meals (% of ingredient as-fed). CM type P-value SECM EPCM EXCM SEM CM type AME, kcal/kg 1974 c 2699 b 3192 a 87 <.0001 Crude protein 27.26 a 24.47 b 23.45 b 0.42 0.0002 Total AA 28.23 a 24.36 b 20.30 c 0.38 <.0001 Lysine 1.76 a 1.53 b 1.17 c 0.02 <.0001 Methionine 0.66 a 0.56 b 0.49 c 0.01 <.0001 Total Sulfur AA 1.34 a 1.17 b 1.05 c 0.02 <.0001 Threonine 1.17 a 1.03 b 1.00 b 0.02 0.0002 Tryptophan 0.47 a 0.44 b 0.35 c 0.00 <.0001

Nutrient Digestibility in Canola Meal for Broilers: AC canola meal fractions vs. parent stock canola meal

The art of fractionation 30 100 ADF, % 25 20 15 Coarse Fraction Fines Fraction Fraction yield, % 80 60 40 20 Fines Fraction Coarse Fraction 10 500 1500 2500 3500 4500 5500 6500 Air-classifier wheel speed, RPM 0 500 1500 2500 3500 4500 5500 6500 Air-classifier wheel speed, RPM Crude protein, % 45 40 35 Fines Fraction Coarse Fraction 30 500 1500 2500 3500 4500 5500 6500 Air-classifier wheel speed, RPM

Table 4. Analyzed nutrient content of the parent stock canola meal compared to the AC fractions. Nutrient Conventional Canola Meal ACCM Fines' fraction ACCM Coarse' fraction Crude Protein 37.92 38.77 36.52 Total Amino Acids 34.57 33.71 32.69 Crude Fat 4.76 4.28 3.23 Crude Fiber 7.24 0.40 2.04 Acid Detergent Fibre 15.49 15.85 21.31 Neutral Detergent Fibre 24.39 23.21 36.60 Calcium 1.33 0.83 1.27 Phosphorus 1.13 1.09 1.08 Lysine 2.15 2.08 2.03 TSAA 1.63 1.60 1.51

Table 5. ATTD of GE and AID of CP and AA in a sample of solventextracted canola meal and two fractions produced through air classification Ingredient P-value FPS CM Fines Coarse SEM Ingredient Gross energy 76.87 a 49.36 b 48.84 b 3.73 0.0004 Crude protein 101.42 a 91.61 b 96.33 ab 2.18 0.0303 Total AA 92.56 a 86.22 b 89.66 ab 1.47 0.0368 Lysine 88.66 85.89 87.35 1.47 0.4152 Methionine 98.34 92.53 95.48 1.58 0.0655 Total Sulfur AA 93.59 a 85.33 b 87.53 ab 2.20 0.0487 Threonine 82.74 a 74.41 b 79.11 ab 2.57 0.0813 Tryptophan 85.07 81.40 82.43 1.41 0.1899

Table 6. AME and ileal digestible CP and AA content in a sample of solvent-extracted canola meal and two fractions produced through air classification Ingredient P-value FPS CM Fines Coarse SEM Ingredient AME, kcal/kg 3133 a 2046 b 1999 b 153 0.0004 Crude protein 38.46 a 35.52 b 35.18 b 0.82 0.0345 Total AA 32.00 a 29.07 b 29.31 b 0.49 0.0031 Lysine 1.91 a 1.79 b 1.78 b 0.03 0.0172 Methionine 0.73 a 0.68 b 0.66 b 0.01 0.0029 Total Sulfur AA 1.53 a 1.37 b 1.32 b 0.04 0.0038 Threonine 1.31 a 1.15 b 1.17 b 0.04 0.0174 Tryptophan 0.50 a 0.45 b 0.40 c 0.01 <.0001

Nutrient Digestibility in Canola Meal for Broilers: B. Napus vs. B. juncea

Table 7. ATTD of GE and AID of CP and AA in B. juncea meal compared to B. napus meal for broilers. Canola meal type P-value B. juncea B. napus SEM CM type Gross energy 64.75 55.84 3.05 0.0586 Crude protein 76.95 72.40 1.58 0.0558 Total AA 79.89 78.19 1.83 0.4037 Lysine 75.90 77.46 1.79 0.4605 Methionine 85.94 86.51 1.61 0.7374 Total Sulfur AA 78.24 76.80 1.51 0.3670 Threonine 72.44 72.67 2.37 0.9028 Tryptophan 86.16 84.67 1.78 0.4366

Table 8. AME and ileal digestible CP and AA content in B. juncea meal compared to B. napus meal for broilers (% of ingredient as-fed). Canola meal type P-value B. juncea B. napus SEM CM type Gross energy 2944 2543 139 0.0603 Crude protein 29.73 28.37 0.61 0.1079 Total AA 26.77 27.23 0.62 0.5008 Lysine 1.52 1.56 0.04 0.3962 Methionine 0.65 0.64 0.01 0.4169 Total Sulfur AA 1.31 a 1.21 b 0.02 0.0102 Threonine 1.17 1.17 0.04 0.8966 Tryptophan 0.37 0.35 0.01 0.0527

Canola meal inclusion and broiler performance: Effect of graded inclusion of B. Napus vs. B. juncea meals

Our approach Pens of mixed sex broilers (44/pen) d0 Pen weight d11 Pen weight Feed disappearance d22 Pen weight Feed disappearance d35 Pen weight Feed disappearance Starter phase ADG ADFI G:F Grower phase ADG ADFI G:F Finisher phase ADG ADFI G:F

Our approach (cont d) Dietary regimens consisted of phase-specific diets containing 0%, 10%, 20% or 30% of either B. napus or B. juncea All diets were formulated to have similar levels of AME and digestible AA within phase Target energy levels lower than recommended

Table 9. Overall growth performance of mixed-sex broilers fed diets containing graded inclusion levels of B. napus or B. juncea meal (d0-35) Dietary inclusion level of CM, % 0% B. napus B. juncea P-value 10% 20% 30% 10% 20% 30% Diet Wt, d 35 2284 2236 2282 2269 2300 2312 2261 0.4865 ADG 61.9 60.7 62.0 61.7 62.5 62.9 61.4 0.7677 ADFI 106.8 107.1 106.7 107.3 107.5 107.9 108.4 0.9028 GF 0.614 0.607 0.625 0.616 0.621 0.614 0.606 0.7364

Table 10. Carcass wt and dressing % of mixed-sex broilers fed diets containing graded inclusion levels of B. napus or B. juncea meal Dietary inclusion level of CM, % 0% B. napus B. juncea P-value 10% 20% 30% 10% 20% 30% Diet AM Wt, g 2176 2209 2222 2155 2203 2123 2160 0.4903 Carcass Wt, g 1518 1511 1514 1502 1512 1504 1499 0.2617 Dressing, % 0.697 0.694 0.695 0.690 0.694 0.689 0.687 0.2259

Table 10. Yield of saleable carcass components from mixed-sex broilers fed diets containing graded inclusion levels of B. napus or B. juncea meal 0% Dietary inclusion level of CM, % B. napus B. juncea P-value 10% 20% 30% 10% 20% 30% Diet P. major 0.240 b 0.253 a 0.253 a 0.251 a 0.247 ab 0.254 a 0.254 a 0.0119 P. minor 0.050 c 0.053 ab 0.053 ab 0.054 ab 0.053 ab 0.052 bc 0.055 a 0.0066 Thighs 0.177 0.174 0.175 0.177 0.180 0.175 0.178 0.8588 Drumsticks 0.140 0.137 0.139 0.139 0.139 0.139 0.136 0.5934 Wings 0.110 0.110 0.110 0.115 0.110 0.112 0.113 0.8396 Total saleable 0.716 b 0.726 ab 0.729 a 0.736 a 0.730 a 0.733 a 0.737 a 0.0454

Table 11. Income over feed costs for mixed-sex broilers fed diets containing graded inclusion levels of B. napus or B. juncea meal 0% Dietary inclusion level of CM, % B. napus B. juncea P-value 10% 20% 30% 10% 20% 30% Diet $/bird placed 2.64 abc 2.58 c 2.63 bc 2.59 c 2.72 ab 2.74 a 2.59 c 0.0037 $/bird placed (quota-adjusted) 1.92 bc 1.86 c 1.90 c 1.87 c 1.98 ab 2.01 a 1.89 c 0.0009 $/ bird marketed 2.73 bc 2.66 d 2.74 bc 2.70 cd 2.80 ab 2.86 a 2.74 bcd 0.0001 $/ bird marketed (quota-adjusted) 1.98 c 1.92 d 1.98 c 1.96 cd 2.04 ab 2.09 a 2.00 bc 0.0001

So what have we learned 1. Oil extraction process has a large impact on digestible nutrient content of resulting meal Importance of knowing origin of the CM Could extruding meal improve digestibility??? 2. Despite successfully separating CM into two fractions differing in crude fiber, no detectable improvement in digestible nutrient content

So what have we learned 3. Does not appear to much difference in nutrient digestibility b/t B. napus and B. juncea GE / AME exception? 4. B. napus and B. juncea can both be included at up to 30% of broiler diets without adverse impact on performance Opportunity for real cost savings, especially with B. juncea

Acknowledgements Funding: Technical assistance: Emily Graham Breanne Chmilar Krishna Kandel Faculty, staff and students at PRC