ISO/CEN standards for Campylobacter - recent developments - Enne de Boer Food and Consumer Product Safety Authority (VWA) The Netherlands

ISO/CEN standards for Campylobacter - recent developments - Enne de Boer Food and Consumer Product Safety Authority (VWA) The Netherlands

Development of international standards International Organization for Standardization (ISO) National standardization institutes (e.g. NMKL, NEN, DIN, AFNOR) Technical committee ISO/TC 34 Agricultural food products Subcommittee SC 9 Microbiology convenor: Mr. Bertrand Lombard; Secretary: Mrs. Sandrine Espeillac CRLCampy.2007.ISO 2

Development of international standards Comité Européen de Normalisation (CEN) Technical committee CEN/TC 275 Food analysis - Horizontal methods Working group (WG) 6 Microbial contamination convenor: Mr. Alexander Leclerq; Secretary: Mrs. Sandrine Espeillac CRLCampy.2007.ISO 3

Development of international standards - New work item proposal (NWIP) 3 months vote - Working Document (WD) - Committee Draft (CD) 3 months vote - Draft International Standard (DIS) 5 months vote - Final Draft International Standard (FDIS) 2 months vote - International Standard - Systematic Review after 3 or 5 years; Revision CRLCampy.2007.ISO 4

Development of international standards Meetings ISO/TC 34/SC 9 and CEN/TC 275/WG 6, April 2007, Cairo CRLCampy.2007.ISO 5

ISO/CEN standards for Campylobacter ISO 10272 ISO 10272, first edition, 1995 Microbiology of food and animal feeding stuffs Horizontal method for detection of thermotolerant Campylobacter Enrichment: - Preston broth, 18h 42ºC, microaeroob - Park & Sanders broth, 4h 37ºC microaeroob, + antibiotic, 2h 37ºC, 40-42h 42ºC microaeroob Plating: Confirmation - Karmali agar - 2nd medium CRLCampy.2007.ISO 6

ISO/CEN standards for Campylobacter EN ISO 10272-11 ISO 10272-1, first edto edition, 2006 Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method Enrichment: Bolton broth 4-6h 37ºC, 40-48h 41,5ºC microaeroob CRLCampy.2007.ISO 7

ISO/CEN standards for Campylobacter EN ISO 10272-11 ISO 10272-1, first edto edition, 2006 Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method Plating: -mccd agar -2nd medium of own choice CRLCampy.2007.ISO 8

ISO/CEN standards for Campylobacter EN ISO 10272-11 ISO 10272-1, first edto edition, 2006 Confirmation o Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method - Morphology - Motility - Microaerobic growth at 25ºC - Aerobic growth at 41,5ºC - Oxidase CRLCampy.2007.ISO 9

ISO/CEN standards for Campylobacter ISO/TS 10272-22 ISO/TS S 10272-2, first edto edition, 2006 Microbiology of food and animal feeding stuffs Horizontal method for detection and enumeration of thermotolerant Campylobacter spp. Part 2: Colony-count technique Colony counting on mccd agar CRLCampy.2007.ISO 10

ISO/CEN standards for Campylobacter ISO/TS 10272-3 There e is a need for the quantitative tat assessment e of Campylobacter in monitoring the effect of control measures and in risk assessment studies. Besides direct plating according to ISO/TS 10272-2 2, semi- quantitative detection may be applied to give an estimate of the level of Campylobacter contamination. CRLCampy.2007.ISO 11

ISO/CEN standards for Campylobacter ISO/TS 10272-3 Draft ISO/TS 10272-3 is based on NMKL method No. 119 (3.Ed.,2007): Thermotolerant Campylobacter. Detection, semi-quantitative and quantitative determination in foods and drinking water. This NMKL method is validated in a collaborative study (2005) Rosenquist, H. et al. (2007), Int. J. Food Microbiol. 118, 201-213. The method showed satisfactory results for fresh chicken, but not for milk due to a relatively low sensitivity and selectivity CRLCampy.2007.ISO 12

ISO/CEN standards for Campylobacter ISO/TS 10272-3 ISO/TC34/SC9 Prague, 2006 Resolution 282: SC9 members agreed to base part 3 of ISO 10272 on the semiquantitative NMKL method 119, excluding milk from the scope, and include the results from their collaborative studies in an annex as performance characteristics. Publication is expected in 2008 CRLCampy.2007.ISO 13

ISO/CEN standards for Campylobacter ISO/TS 10272-3 Principle: The test portion and decimal dilutions thereof are inoculated or diluted in Bolton broth and homogenized Incubation: 4-6h 37ºC, 40-48h 41,5ºC, microaerobic Plating on mccd agar, further as in ISO 10272-1 This method is based on qualitative detection in selected dilutions; the result is therefore given in intervals. CRLCampy.2007.ISO 14

ISO/CEN standards for Campylobacter ISO 10272-3 Interpretation of growth in the semi-quantitative assessment of Campylobacter Dilution growth on mccd agar plates 10 1 - + + + + + 10 0 - - + + + + 10-1 - - - + + + 10-2 - - - - + + 10-3 - - - - - + Interpretation (cfu/ml) <0,1 0,1-1- 10-100- 1000-1 10 100 1000 10.000000 CRLCampy.2007.ISO 15

ISO/CEN standards for Campylobacter ISO 10272-4 Part 4 of ISO 10272 will include the detection of Campylobacter in samples from the primary production stage. A first draft will be sent to SC 9 members in 2008. Project leaders: Janet Corry (UK), Wilma Jacobs-Reitsma (NL) CEN/TC275/WG6/TAG5: Working Group Primary Production Stage CRLCampy.2007.ISO 16

CEN mandate M/381 Standardized validated methods are of major importance to ensure uniform application and control of Community legislation in the EU. All reference methods included in EU legislation should be standardized and validated. Therefore a mandate for standardization has been addressed to CEN in the field of methods of analysis of foodstuffs ff concerning food hygiene. CRLCampy.2007.ISO 17

CEN mandate M/381 Elaboration at o of validated CEN standards: ds -Food borne viruses - Pathogenic Vibrio -Enterobacteriacea - Listeria monocytogenes -E E. coli O157 - Campylobacter -Salmonella in faeces - Yersinia enterocolitica -Histamine - Staphylococcal enterotoxin -Bacillus cereus enterotoxin - Enterobacter sakazakii CRLCampy.2007.ISO 18

CEN mandate M/381 Validation of EN ISO 10272-1 and adso/s ISO/TS 10272-2 Project leaders: Wilma Jacobs-Reitsma, Enne de Boer In collaboration with CRL Campylobacter Start: autumn 2008 (expected) CRLCampy.2007.ISO 19

CEN mandate M/381 Outline of inter-laboratory ato studies in the framework of the CEN mandate Quantitative studies -at least 10 participants -4 levels of contamination: blank, low, intermediate, high -2 blind replicates per level -5 different matrices in case of horizontal application of the method -So a total of 40 samples per laboratory CRLCampy.2007.ISO 20

CEN mandate M/381 Outline of inter-laboratory ato studies in the framework of the CEN mandate Qualitative studies -at least 13 participants -3 levels of contamination: blank, low, high -6 blind replicates per level -5 different matrices in case of horizontal application of the method -So a total of 120 samples per laboratory CRLCampy.2007.ISO 21

CEN mandate M/381 Wanted! Participating i labs CRLCampy.2007.ISO 22