Developing Machine-Harvestable Fresh Market Tomatoes; and other Highlights from the UF Breeding Program

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Developing Machine-Harvestable Fresh Market Tomatoes; and other Highlights from the UF Breeding Program S.F. Hutton, J.W. Scott, B.M. Santos 813-633-4137 sfhutton@ufl.edu

Comparison of once-over harvest yields of compact growth habit (CGH) tomato inbreds grown at GCREC, Balm, spring 2012 z. Genotype Total (25 lb box/a) XL (25 lb box/a) Fla. 8916 2514 a 1076 a Fla. 8607 2296 ab 750 b Fla. 8834 2139 bc 274 c Fla. 8107 2008 bc 658 b Fla. 8914 1965 c 362 c z Split-plot design with varying nitrogen rates as main plot; 10 plants per plot at 1-ft spacing; mean separation by LSD

Once-over harvest yield of compact growth habit (CGH) tomato inbreds compared to Florida 47 on a grower farm, winter 2013 z. Genotype 25 lb box/acre 25 lb box/bin Florida 47 490 33.26 Fla. 8916 1359 32.36 Fla. 8916a 1476 30.76 Fla. 8916b 1482 31.87 Fla. 8915 900 25.72 Fla. 8914 490 24.50 Fla. 8834 660 33.00 z 8916, 8916a, 8916b grown on 0.31 A, other CGH lines grown on 0.1 A, Florida 47 grown on 11.25A.

Spring 2013

2014-2015 Specialty Crop Block Grant Main Objectives: 1 Improve jointless, CGH germplasm 2 Determine best cultural practices 3 Assess labor efficiency of mechanical harvest 4 Evaluate feasibility/profitability of adopting CGH varieties and mechanical harvesting

2014-2015 Specialty Crop Block Grant Main Objectives: 1 Improve jointless, CGH germplasm 2 Determine best cultural practices 3 Assess labor efficiency of mechanical harvest 4 Evaluate feasibility/profitability of adopting CGH varieties and mechanical harvesting

2014-2015 Specialty Crop Block Grant Breeding Objectives: Develop improved parents and hybrids Integrate key traits TYLCV, FCR, TSWV resistances Heat-tolerance Graywall resistance

Program Highlights Short term goals: Improved inbreds and hybrids Yield, size, % marketable, firm, etc. With key resistances (TYLCV, FCR, TSWV) Heat tolerance Long term goals: Bacterial spot and bacterial wilt resistance TYLCV gene discovery and characterization Graywall resistance Whitefly resistance Compact growth habit, jointless-pedicel

Program Highlights Short term goals: Improved inbreds and hybrids Yield, size, % marketable, firm, etc. With key resistances (TYLCV, FCR, TSWV) Heat tolerance Long term goals: Bacterial spot and bacterial wilt resistance TYLCV gene discovery and characterization Graywall resistance Whitefly resistance Compact growth habit, jointless-pedicel

F 1 Fla. 8383 Fla. 7776 Ty-6 Mapping Genotyping through SolCAP Community Project Subset of 384 SNPs optimized for processing and fresh market 158 polymorphic SNPs for (7776 x 8383) population

F 1 Fla. 8383 Fla. 7776 120 100 80 13/13 markers P <.0001 F Value 60 40 20 0 Chr: 1 2 3 4 5 6 7 8 9 10 12

Chromosome 6 Ty-1 and Ty-3 46 Mb Ty-1, Ty-3 Solanum chilense introgressions Ty-3 minimal introgression

Marketable yield, extra-large yield, fruit size and culls for tomato inbreds and control hybrids in spring 2013 at GCREC Marketable yield (25 lb. box/a) Fruit size Culls Entry Total Extra-large (oz.) (% by wt.) 8872B 1904 a z 1648 a 8.1 a 31 bc 7781 1884 a 482 d 4.9 f 27 c E228 bwt 1777 a 825 b-d 5.9 c-e 22 c E249 TSWV 1743 a 981 b-d 6.2 cd 25 c 8869 1700 a 924 b-d 6.1 cd 37 a-c Solar Fire 1639 ab 608 cd 5.3 ef 30 bc 8921 (sister) 1611 ab 642 cd 5.6 de 33 a-c 8923B 1513 ab 1308 ab 7.9 a 32 a-c 8925 1472 ab 1116 bc 7.0b 35 a-c Sanibel 1366 ab 981 b-d 6.5 bc 44 ab Sebring 1057 b 664 cd 6.2 cd 45 ab Florida 47 1028 b 544 d 5.7 de 48 a z Mean separation in columns by Duncan s multiple range test at P < 0.05.

Marketable yield, extra-large yield, fruit size and culls for tomato inbreds and control hybrids in spring 2013 at GCREC Marketable yield (25 lb. box/a) Fruit size Culls Entry Total Extra-large (oz.) (% by wt.) 8872B 1904 a z 1648 a 8.1 a 31 bc 7781 1884 a 482 d 4.9 f 27 c E228 bwt 1777 a 825 b-d 5.9 c-e 22 c E249 TSWV 1743 a 981 b-d 6.2 cd 25 c 8869 1700 a 924 b-d 6.1 cd 37 a-c Solar Fire 1639 ab 608 cd 5.3 ef 30 bc 8921 (sister) 1611 ab 642 cd 5.6 de 33 a-c 8923B 1513 ab 1308 ab 7.9 a 32 a-c 8925 1472 ab 1116 bc 7.0b 35 a-c Sanibel 1366 ab 981 b-d 6.5 bc 44 ab Sebring 1057 b 664 cd 6.2 cd 45 ab Florida 47 1028 b 544 d 5.7 de 48 a z Mean separation in columns by Duncan s multiple range test at P < 0.05.

Total marketable yield, extra-large marketable yield, fruit size and culls for tomato hybrids in spring 2013 at GCREC. Marketable yield (25 lb. box/a) Fruit size Culls Entry Total Extra-large (oz.) (% by wt.) 8904 2524 a z 1673 a 6.6 a 14 e 8314 Ty1 2493 a 913 e-h 5.2 cd 19 de 8884 2388 ab 1609 ab 6.5 ab 18 de Tasti-Lee Sw7 2307 a-c 783 f-h 5.1 cd 20 de 8905 BWT 2238 a-d 1403 a-c 6.0 a-c 13 e Phoenix 2206 a-d 1304 b-d 6.2 a-c 20 de E126 2159 a-e 1150 c-e 5.7 a-d 28 a-d 8897 Ty1 2152 a-e 893 e-h 5.4 b-d 20 de E126 Ty1, Frl 2148 a-e 950 d-h 5.5 a-d 18 de 8897 2075 a-e 995 d-f 5.5 a-d 25 b-e Tasti-Lee 1820 b-f 739 g-h 5.3 b-d 21 de Tasti-Lee Ty1 1687 c-f 343 i 4.8 d 22 c-e Solar Fire 1658 d-f 675 hi 5.4 b-d 30 a-d 8314 1652 d-f 770 f-h 5.5 a-d 38 a Florida 47 1548 e-g 965 d-g 6.1 a-c 34 a-c Tygress 1426 fg 873 e-h 5.8 a-c 37 ab Sebring 986 g 632 hi 6.2 a-c 40 a z Mean separation in columns by Duncan s multiple range test at P < 0.05.

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