INTERNATIONAL UNION FOR THE PROTECTION OF NEW VARIETIES OF PLANTS TECHNICAL WORKING PARTY FOR ORNAMENTAL PLANTS AND FOREST TREES. Forty-Fifth Session

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ORIGINAL: English DATE: July 10, 2012 INTERNATIONAL UNION FOR THE PROTECTION OF NEW VARIETIES OF PLANTS Geneva E TECHNICAL WORKING PARTY FOR ORNAMENTAL PLANTS AND FOREST TREES Forty-Fifth Session Jeju, Republic of Korea, August 6 to 10, 2012 ADDENDUM VARIETY DESCRIPTION DATABASES Document prepared by an expert from France The Annex to this document contains a copy of a presentation, prepared by an expert from France for the forty-fifth session of the Technical Working Party for Ornamental Plants and Forest Trees, concerning reference collections and grouping characteristics, using the example of Pea. [Annex follows]

ANNEX Reference Collection and grouping characteristics Example of the Pea species 1 Monterey (USA, 2011) : TWV/45/24 Partial Revision of the Test Guidelines for Pea (TG/7/10), TWV/45/6 Variety Descriptions Databases TWV/45/13 Concept of a Database Containing Pea Variety Descriptions Goals : to define the theoretical collection of varieties of common knowledge to collect a maximum of descriptions of these varities to define reliable characteristics for use as excluding characterisitics = strong and discriminant charactéristics Aim :To obtain a Common Working Data Base Helpful to chose reference varieties in DUS examination. 2

Variétal denomination EU CPVO UPOV OECD PMA EU Actual Maintainer EU 1st Registration EU 1st Maintainer EU Last withdrawal EU Last Maintainer EU Additional extension ) CPVO Applicant CPVO Status UPOV Applicant UPOV 1983.11.22 Cebeco Handelsraad (NL) FR #5147 DARK SKIN UPOV 1975.11.13 Canners Seed Corporation US PERFECTION #6060 FREEZER UPOV 1975.11.14 Canners Seed Corporation US #7025 EARLY SWEET UPOV 1975.11.10 Canners Seed Corporation US #8221 EARLY UPOV 1975.06.30 Canners Seed Corporation US PERFECTION #8615 EARLY UPOV 1980.03.04 Canners Seed Corporation US PERFECTION #8617 EARLY UPOV 1975.05.20 Canners Seed Corporation US PERFECTION OECD Applicant PMA Applicant CPVO Application date UPOV Application date UPOV Country OECD Appli. N PMA Application date PMA Status TWO/45/6 Add. Annex, page 2 Reference collection OECD Pea theoretical collection = 3 127 varieties 3 FRANCE-UPOV TWV, BMT, Venlo Brasilia 2012 2011 envisaged for excluding Current grouping characteristics i.e. anthocyanin coloration, number of nodes up to and including first fertile node, hilum color New potential grouping characteristics i.e. fasciation, time of flowering, resistance 2 excel files have sent in order to know information (synonym, market #9888 FREEZER UPOV 1978.04.24 Nunhems Zaden B.V. US If the variety should appear in the technical collection, If description or/and seeds are available. The note of each characteristic for each variety. 4

Annex, page 3 Argentina, Australia, Bulgaria, Canada, China, Czech Republic, Germany, Denmark, Estonia, France, United Kingdom, Hungary, Japan, Kenya, Republic of Korea, Republic of Moldova, Netherlands, New Zealand, Poland, Portugal, European Union, Romania, Russian Federation, Slovakia, Ukraine, United State of America & South Africa have been contacted. At this time, 11 countries (2/5) answered this study and sent us descriptions in the following proportion : 5 887 descriptions received, concerning 2467 varieties more than 78% of the theoretical collection 5 Properties of individual characters Use Number of time this characterisric is described / total number of described varieties Discriminating power % of excluded varieties on the basis of the character Distortion Number of differences between descriptions concerning the same variety 6

Annex, page 4 Use of the characteristics 7 Discraminating power Qualitative characteristics Quantitative characteristics 8

Annex, page 5 Distortion (1/2) Analysis performed only on varieties for which we received more than one description. Qualitative characteristic Number of varieties with # notes Total number of varieties Percentage of distortion 1 Anthocyanin coloration 11 1798 0.61 52 Color of cotyledon 149 1784 8.35 8 Presence of leaflets 33 1796 1.84 41 Pod extremity 108 1784 6.05 58.1 Fusarium 1 81 1126 7.19 60 Resistance to Ascochyta 12 328 3.66 Obviously, some differences are due to errors in notes transcriptions, using wrong guidelines or observations of wrong variety. Contact will be made to clarify some issues 9 Distortion (2/2) Quantitative characteristic Number of varieties with # notes Total number of varieties Percentage of distortion Difference between varieties 1 note 5 Number of Nodes 1271 1378 92.24 900 65.31 4 Stem lengh 1206 1381 87.34 805 58.29 42 Pod curvature 1110 1351 82.16 511 37.82 57 Seed weight 1135 1383 82.07 660 47.72 47 Immature seed color 25 Number max of flower 830 1255 66.14 473 37.69 457 1325 34.49 164 12.38 10 2 notes 369 26.78 409 29.62 174 12.88 260 18.80 104 8.29 60 4.53 3 notes 93 6.75 174 12.60 44 3.26 92 6.65 50 3.98 For these continuous characteristics, special attention must be paid in term of distances between varieties 31 2.34

Annex, page 6 To conclude, it s possible to characterize each character to help the experts to choose the best For official grouping characters Use Discriminating power Distortion 1 Anthocyanin coloration 95.26 95/5 0.61 5 Number of nodes 78.51 54 ->4/5/6 26.78* 8 Presence of leaflets 97.50 39/61 1.84 20 Flecking on stipules 90.69 2/98 3.09 39 Pod parchment 85.17 7.5/92.5 14.04 40 Pod thickened wall 9.80 84/16 4.57 41 Pod extremity 97.21 24/76 6.05 43 Pod color 93.49 0.2/98/1 2.83 47 Immature seed color 77.36 33 ->4/5/6 8.29* Use Discriminating Distortion power >75% < [60 40] < 10 [60 40] / [80 40% 75% 20] 10 20 <40% >[80 20] >20 49 Type of strach grains 94.24 51/49 3.63 52 Color of cotyledon 94.36 62/38 8.35 53 Seed marbling of testa 57.09 97/3 1.16 54 Pink spots on testa 36.03 91/5/4 14.58 55 Hilum color 89.08 98/2 1.60 58_1 Fusarium 1 57.19 80/20 7.19 *2 notes 11 For candidate grouping characters Use Discriminating power Distortion 3 Fasciation 86.80 99/1 0.57 4 Stem lengh 79.65 49 ->4/5/6 29.62* 6 Color of foliage 86.90 2/93/5 8.07 24 Time of flowering 84.75 68 ->4/5/6 10.07* 25 Max number of flower 74.20 17 ->4/5/6 4.53* Use Discriminating Distortion power >75% < [60 40] < 10 [60 40] / [80 40% 75% 20] 10 20 <40% >[80 20] >20 26 Color of wing 4.09 80/20 5.43 37 Pod length 83.90 16 ->4/5/6 14.66* 38 Pod width 83.01 55 ->4/5/6 42.21* 42 Pod curvature 79.45 27 ->4/5/6 12.83* 44 Pod intensity color 88.77 46 ->4/5/6 13.80* 45 Pod suture strings 18.33 95/5 2.37 48 Seed shape 88.74 37/45/4/14 44.91 57 Seed weight 77.34 47 ->4/5/6 18.80* 59 Resistance to Erysiphae 53.47 87/13 3.39 60 Resistance to Ascochyta 32.87 80/20 3.66 Plant : habit 2.84 70 ->4/5/6 0* Pod : type curvature 23.95 98.9/1/0.1 1.89 Seed : Dimpled 22.12 75/25 44.34 Virus 2 27.45 60/40 15.00 *2 notes 12

Annex, page 7 Among 3127 varieties present in the theroretical collection, 667 ones are without description after this study : 103 from the EU catalogues including 48 non-withdrawn ones countries in charge of the admission should be contacted 13 from CPVO 415 from UPOV (PLUTO database) 108 from OECD 21 are EU Provisional Market Authorization 13 How to go on What is it possible to do? Relation for number of nodes between UK and FR on 875 varieties Number of nodes Percentage of varieties (Difference UK/FR) 6 0.11 5 0.11 4 1.03 3 16.46 2 49.83 1 29.60 0 2.63 1 0.11 2 0.11 ) ) 80 % ) Except for some errors, (to be clarified), more than 80 % of the varieties have one or two notes less in UK than in FR. 14

Annex, page 8 How to go on Correlation between 2 characters can be examined variable graph (pois) Dim 2 (8.61%) -1.0-0.5 0.0 0.5 1.0 X47.Immature.seed.colour X44.Pod.intensity.colour X49.Type.of.strach.grains X8.Presence.of.leaflets Virus.2 X38.Pod.width X48.Seed.shape X37.Pod.length X4.Stem.lengh Seed...Dimpled -1.5-1.0-0.5 0.0 0.5 1.0 1.5 Dim 1 (14.09%) On the basis of the actual data, it seems that 58 % of the variabilité of the characteristic «Time of flowering» could be explained by the characteristic «Number of nodes» X1.Anthocyanin.coloration X5.Number.of.nodes X55.Hilum.colour X54.Pink.spots.on.testa X57.Seed.weight X24.Time.of.flowering X53.Seed.marbling.of.testa X41.Pod.extremity X52.Colour.of.cotyledon Timeflowering 2 4 6 8 R²Pearson=0.5842 15 2 4 6 8 Nbnodes THANK YOU 16