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Report No 4 ^) \ * -^ ' '^; A REPOR ON RATTAN SHOO PROCESSNG ECHNQUES by Ms Pannee Denrungruang PD 24100 Rev 10) : Promotion of Sustainable Utilization of Rattan from Plantation in Thailand Royal Forest Department Bangkok 10900 THAILAND November 2002

I CONTENTS Page Abstract Introduction Objective Materials and Methods Results and Discussions Acknowledgement References 2 3 3 6 12 12

RATTAN SHOOT PROCESSING TECHNIQUES by Pannee Denrungruang comments by Shoot Processing Consultant ABSTRACT There are more than 60 types of young rattan in Thailand but the only Calamus sp is one of the most favorable food in the Northeast region The most popular rattan in Sakon Nakhon province is Calamus sp or locally called "Wai Dong" This species have medium stern diameter about 2-25 cm Due to that qualities the study about rattan shoots processing in canning is set in order to presenttheirfood preservation qualities and also extension approach to the people who are the owner of rattan shoot plantation or interested in rattan shoot production The study conducted by using glass bottle size 6 0z in brine of 2 percents and syrup 30 percents concentration adjusting ph below 45 by citric acid The products of rattan shoots in brine and syrup will be storage in room temperature for microorganism examination for 3 and 6 months There is no microorganism founds in rattan product The detailis shown in table I The nutritive value of rattan products showed little change from fresh rattan shoot in protein and fat The energy (Kcal) of rattan shootin syrup is more than rattan shootin brine and fresh rattan Because sucrose in syrup give energy The dehydration product is better in texture and color The result of sensory evaluation between two treatments rattan shoot in brine and rattan shoot in syrup are shown in table 3 From the table 3 means of sensory evaluation is between 369 to 490 So the result is between neutral to like slightly It means that the products is accepted by panelists Key words : Calamus sp rattan shoots processing rattan shoots in brine rattan shoots in syrup

2 INTRODUCTION Rattans are climbing palms that have been utilized for centuries in several Asian countries and their economic important is second to the forest trees Rattans are mainly used to make furniture house hold utensils and home decorators However the use of rattan shoots as vegetable has been mentioned in many areas According to Renuka C (2001) young leaves roots and shoottips of rattan has been used as medicines and as a vegetable by some indigenous people in Bangladesh Recently rattan shoot are popularly consumed and sold in some local areas Instead of waiting for 6-7 years to obtain the cane for utilization rattan shoot can be harvested just within the second year after planted Using rattan shoot as an ingredient in many kinds of food is becoming popular especially in the Northern Northeastern and Central part of Thailand Moreover Lao and Vietnam " are some of our neighboring countries that consume plenty of rattan shoots and demands for rattan shoot seem to be increased in nearly future It is can be foreseen that local market and international market of rattan shoots are tending to be expanded The most popular rattan species planted for shoots in Thailand are Calamus siamens/'s (Wai Nam Rung) and C vi'minalis (Wai Dong) that are bitter tasted At present fresh shoot of rattan become a popular souvenir from Northeastern while people in Central started to know how to cook these shoots for food Rattans are very useful in pharmaceutical Some types of rattans for example the ashes of stem of Calamus ornatus Blume are used in treating chromic or returning yaws An infusion of the root is drunk to lessen pain in child-birth and ethanol (50%) extract of aerial parts antianeer (Burkill I H 1966) Calamus L acathophyllus Becc is prescribed for treating malaria and jaundice; the roots are part of a compound preparation against nausea (Perry L M 1980) In central and south India found that the Calamus rotang Linn the roots are astringent acrid

" 3 bitter cooling expectorant alexeteric antidysenteric hypotensive depurative antiinflammatory diuretic febrifuge and tonic The leaves are acrid bitter cooling astringent and laxative and are useful in vitiated conditions of pitta skin diseased leprosy and pruritus The seeds are astringent acrid sour depurative and expectorant and are useful in vitiated conditions of kapha cough skin diseases and pruritus The sprouts are acrid sweet and thermogeni and are useful in tithed condition of vata and kapra (Sala 1994) OBJECTIVES To develop techniques for processing packaging and chemical properties of rattan shoot products MATERIALS AND METHODS Raw material Calamus sp (vaidong) shoots Chemicals Sodium metabisulphite (Na S 0 ) citric acid calcium chloride (CaCl ) sodium chloride (NaCl) and sugar Equipment Oven autocave kitchen utensils gas cooker glassware etc Test methods Microorganism examination of rattan shoot in brine and syrup products after 3 and 6 months period analyses by Department of Medical Science Ministry of public Health (table I) The Nutritive value analysis of rattan shoot and rattan products analyses by Nutrition Division Department of Health Ministry Public Health Bangkok (table 2) Physical properties test after 6 months (table 3)

" 4 Methods I The rocessin bottled rattan shoots in brine solution Rattan shoots (Calamus v/'minalis) (fig I) were bought from the farmers Rattan shoots are peeled the leaf-sheaths and skin by knife (fig 2) Rattan shoots are opaque white colour They are cut in pieces long about 3 % inches and diameters are varies from I ^ - }^ inch depend on size of raw material In this cutting process rattan shoots were in 01 % sodium metabisulphite The prepared rattan shoots were boiled for 10 minutes in blanching solution (fig 3) The blanching solution contained 01% sodium metabisu!phite and 02% citric acid in water followed by cooling in 01 percent sodium metabisulphite solution Rattan shoots were put in the 6 0z glass bottles (fig 4) and the filled with boiling brine solution The brine solution contained 2% salt 05% citric acid and 01% calcium " " chloride in water The headspace is about I ^ inch in the top of the glass bottle Samples were exhausting process the open glass bottles were heated in boiling water bath about 15 minutes and closed the lids (fig 5) After exhausting put the o glass bottles into boiling water (100 C) about 30 minutes (fig 6) The water must boil continuously during the entire processing The bottled rattan shoot industry the processing should be done in an autoclave under the steam pressure of 10 pounds per inch (fig 7) in 15 minutes for 6 0z bottle instead of taking 30 minutes in processing under boiling water Storage in room temperature (fig 8) - L

5 2 The rocessin bottled rattan shoots in s ru solution Rattan shoots (Calamus viminal^^) were bought from the farmers Rattan shoots are peeled the leaf-sheaths and skin by knife Rattan shoots are opaque white colour They are cut in pieces long about 3 I ^ inches and diameters are varies from I ^ - }^ inch depend on size of raw material In this cutting process rattan shoots were in 01 % sodium metabisulphite The prepared rattan shoots were boiled for 10 minutes in blanching solution The blanching solution contained 01% sodium metabisulphite and 02% citric acid in water followed by cooling in 01 percent sodium metabisu!phite solution Rattan shoots were put in the 6 0z glass bottles and the filled with syrup solution contained 30% sucrose 05% citric acid and 01% calcium chloride in water The headspace is about I ^ inch in the top of the glass bottle Samples were exhausting process the open glass bottles were heated in boiling water bath about 15 minutes and closed the lids After exhausting the sterilized process is used by putthe glass bottles into boiling water o (100 C) about 30 minutes The water must boil continuously during the entire processing Storage in room temperature The bottled rattan shootindustry the processing should be done in an autoclave under the steam pressure of 10 pounds perlnch in 15 minutes for 6 0z bottle instead of taking 30 minutes in processing under boiling water L

6 3 The rocessin sun-d in and dried rattan shoot Rattan shoots (Calamus viminalis) were bought from the farmers Rattan shoots are peeled the leaf-sheaths and skin by knife Rattan shoots are opaque white colour They are cut in pieces long about I }^ inches and divide to small part depend on size of raw material In this cutting process rattan shoots were in 01 % sodium metabisulphite The prepared rattan shoots were boiled for 10 minutes in blanching solution The blanching solution contained 01% sodium metabisulphite and 02% citric acid in water followed by cooling in 01 percent sodium metabisulphite solution After cooling the shoots are drained and spread on mats or trays and keep in the sun for drying The sun-drying product will take three to four days to be dried up However dehydration of rattan shoots at 70 C take about 10 hours (fig 9) And the product is better in texture and color (fig 10) RESULTS AND DISCUSSIONS Rattan shoot which is used for glass bottle sun-drying or dehydration is the soft edible part of the shoot after removing the hard covering part It is white or cream in color with a little bitter characteristic taste The taste which is accepted by consumers According to the result of 3 methods the processing can illustrate shown in scheme I 2 and 3 There is no microorganism founds in bottled rattan shoots in brine and syrup solution after keep it for 3 months and 6 months ~

" 7 Rattan shoots Peeling the leaf-sheaths and skin Cutting Boiling in solution for 10 minutes <-- sodium metabisulphite 01% citric acid 02% Cooling in 01% sodium metabisulphite solution Packing in glass jar Filling solution <- Brine solution Salt 2% Cacj* 01' Citric acid 05% Exhausting for 15 minutes Cooking in boiling water for 30 minutes Storage in room temperature * cacl =Calcium chloride Scheme I The bottled rattan shoots in brine solution

8 Rattan shoots " Peeling the leaf-sheaths and skin Cutting Boiling in solution for 10 minutes ^ sodium metabisulphite 01% citric acid 02% Cooling in 01% sodium metabisulphite solution Packing in glass jar Filling solution < Syrup solution Sucrose 30% Cacj* 01' Citric acid 05% Exhausting for 15 minutes Cooking in boiling water for 30 minutes Storage in room temperature * cacl =Calcium chloride Scheme 2 The bottled rattan shoots in syrup solution

r 9 Rattan shoots Peeling the leaf-sheaths and skin Cutting Boiling in solution for 10 minutes < sodium metabisulfite 01% citric acid 02% Cooling in 01% sodium metabisulfite solution U Spread on mats ortrays for drying Drying in oven at 70 C Sun-drying for 3-4 days aboutlo hours Dried rattan shoot Dried rattan shoot * CaCl = Calcium chloride Scheme 3 Sun-drying and dried rattan shoot

10 T^ b!^ I The analysis of microorganism in the rattan shoot products Name ph Value Bacteria Yeast and Mould n Rattan shoot in syrup 43 Notfound Notfound Rattan shoot in brine 43 Notfound Notfound Note: Acid resistance bacteria (at 37 C and 55 C) o Department of Medical Science Ministry of public Health 2002 o The result of nutritive value offresh rattan and rattan products are shown in table 2 The nutritive value of rattan products showed little change from fresh rattan 11 shoot in protein and fat The energy (Kcal) of rattan shoot in syrup is more than rattan shoot in brine and fresh rattan Because sucrose in syrup give energy The results of the study are good in physical appearance which favorated from 50 random testers moderately marks scale (table 3) Sun-drying and dehydration of the soft edible part of the rattan shoot is about ten percents of the whole rattan shoot The dehydration ratio is 8:1 The rehydration ratio is I :3 Moisture content of the product is 6 percents (U V Moisture tested-

11 Table 2 The Nutritive value analysis of 100 grains edible part " Sample name Fresh Rattan shoot Rattan shoot in brine Rattan shoot In syrup Energy (Kcal) 32 28 80 Moisture (g) 92 92 80 Protein (9) 251 219 320 Fat (9) 062 070 066 Fibre (9) 253 NA NA Vitamin A (RE) 2 3 NA Vitamin Bl (ing) 016 004 NA Vitamin B2 (ing)) 013 007 NA Vitamin C (ing) 14 4 NA I Vitamin E (ing) 045 085 NA Sodium (ing) 108 2240 253 @ Potassium (ing) 361 148 87 Magnesium (ing) 48 33 26 Calcium (ing) 69 40 52 10n (ing) 045 042 021 Zinc (ing) 281 161 151 Copper(ing) 031 014 020 Iodine (microgram) 24 415 44 Note NA = Not Analysis RE = Retinal equivalent(beta-carotene) Nutrition Division Department of Health Ministry Public Health Bangkok 2002

12 Table 3 The sensory evaluation of rattan shoot in brine and rattan shoot in syrup " Product color odor means flavor texture acceptance rattan shoot in brine 380 369 376 380 380 rattan shoot in syrup 398 389 382 409 395 Note : I = dislike very much 2 = dislike slightly 3 = neither like nor dislike 4 = like slightly 5 = like very much U ACKNOWLEDGEMENTS I wish to thank the International Tropical Timber Organization (ITFO) for providing financial support for this project and thank you the Nutrition Division Department of Health Ministry of Public Health Bangkok for the valuable analysis of nutrition REFERENCES I Burkill I H 1966 A Dictionary of the Economic Products of Malay Pennisular Vol I Art Printing works Kuala Lumper 2 Perry L M 1980 Medicinal Plants of East and Southeast Asia : Attributed Properties and uses The MIT Press Massachusetts 620 p L 3 Renuka C 2001 Uriasylva An international of forestry and forest industries Uses of rattan in South Asia V01 52 2001/1 p 7 4 Sala A V 1994 Indian Medicinal Plant Vol I Orient Longman Ltd India 42 p

NDEX 11 - ; " ^> A ~^ $; \ ^ ^ ^ ^"^^^:\ ~ - ~ - t" ^: =S'*ap ^ Fig I Edible rattan shoot Fig 2 Peelthe leaf-sheaths and skin of rattan shoots Fig 3 Blanching rattan shoots Fig 4 Packed rattan shoots in the glass bottles Fig 5 Exhausting rattan shoots product Fig 6 Sterilized rattan shoots product in boiling water

Fig 7 Sterilized rattan shoots product by autoclave Fig 8 Bottled of rattan shoot Fig 9 Dehydration of rattan shoots Fig 10 Dried rattan shoot products by oven