Fragaria vesca L. ssp. americana (Porter) Staudt

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Plant Propagation Protocol for Fragaria vesca ESRM 412 Native Plant Production Protocol URL: https://courses.washington.edu/esrm412/protocols/frve.pdf TAXONOMY Plant Family Scientific Name Rosaceae Common Name Rose family Species Scientific Name Scientific Name Fragaria vesca L. Varieties Fragaria vesca L. var. americana Porter Fragaria vesca L. var. bracteata (A. Heller) R.J. Davis Fragaria vesca L. var. crinita (Rydb.) C.L. Hitchc. Fragaria vesca L. var. alba (Ehrh.) Rydb. Sub-species Fragaria vesca L. ssp. americana (Porter) Staudt Fragaria vesca L. ssp. bracteata (A. Heller) Staudt Fragaria vesca L. ssp. californica (Cham. & Schltdl.) Staudt Fragaria vesca L. ssp. vesca Cultivar None Common Synonym(s) None Common Name(s) Woodland strawberry, Woods strawberry, California strawberry Species Code (as per USDA Plants FRVE database) GENERAL INFORMATION Geographical range

Ecological distribution Climate and elevation range Local habitat and abundance Plant strategy type / successional stage Plant characteristics Distribution maps from the USDA Plants Database. [1] Forests, woods Chaparral Stream banks Meadows [2,3,4,5,6] Moist areas below 7000 m. across North America and Europe [2,3,4,5,6] Widely abundant in moist woods and stream banks of low/mid elevation. [2] Self-propagates via rhizomes and stolons Invasive ground-covering behavior [6] Herbaceous, ground-covering perennial Reproduces via seed and self-cloning Leaves are serrate, basal, and pubescent. Leaflets appear in triplets. Petioles grow to 3-12 cm. Flowers are perfect with 5 white petals, many pistils, and 20-15 stamens. Fruit is a red, fleshy receptacle covered in achenes. [2,6,7] PROPAGATION DETAILS Vegetative Propagation Method Plugs [4,5,6] Ecotype Propagation Goal Plants Propagation Method Vegetative Product Type Container (plug) Stock Type 2-4 inch pot Time to Grow 3 months or less Target Specifications Plugs with well established roots. Propagule Collection Instructions Identify plants that have put out healthy runners and plantlets. Take 4-16 inch cuttings in spring, summer, or fall. Propagule Processing/Propagule Keep cuttings in a cool, moist place.

Characteristics Pre-Planting Propagule Treatments Growing Area Preparation / Annual Practices for Perennial Crops Strike cuttings 1.5 inches deep in sheets of 1:1 perlite to vermiculite mixture. Grow cuttings in a greenhouse with a misting system. Keep plants well-watered. Establishment Phase Details Length of Establishment Phase Active Growth Phase Length of Active Growth Phase Hardening Phase Length of Hardening Phase Harvesting, Storage and Shipping Length of Storage Guidelines for Outplanting / Performance on Typical Sites After 90 days or less, transplant cuttings into individual 2-4 inch containers. Use standard potting mix. Cover roots and stems. 90 days or less. None. 12 weeks. Plugs may be stored in a shadehouse. Up to 4 months. Prepare an area with full sunlight and loose soil. When fall/winter rains begin, plant plugs 10 inches apart. Average transplant survival is around 85%. Plants may be fertilized lightly. Fragaria vesca can exhibit invasive ground-covering behavior; prune back runners regularly. Plant vigor will decline after 3-4 years. Other Comments Vegetative Propagation Method Bareroot [4] Ecotype Propagation Goal Plants Propagation Method Vegetative Product Type Bareroot plants; field-grown Stock Type Bareroot plants Time to Grow 1 year Target Specifications Plants with well-established roots; 5 cm in height. Propagule Collection Instructions Plant healthy, mature individuals on a raised bed. Propagule Processing/Propagule Characteristics Pre-Planting Propagule Treatments Growing Area Preparation / Annual Practices for Perennial Crops The raised bed can contain equal parts of rotted manure, sand, and soil. Establishment Phase Details None. Length of Establishment Phase 2 weeks. Active Growth Phase Water regularly. Keep soil moist. Length of Active Growth Phase 12 weeks. Hardening Phase Length of Hardening Phase Harvesting, Storage and Shipping Harvest runners and plantlets after one year, in Spring or Fall. Cuttings can be immediately outplanted in a

new raised bed or potted in containers for plugs (store in a shadehouse). Length of Storage 4 months if potted for plugs. Guidelines for Outplanting / Performance on Typical Sites New plants must be outplanted on a new raised bed with snowcover. Other Comments Propagation From Seed Method (Least Recommended) [3] Ecotype Propagation Goal Plants Propagation Method Seed Product Type Container (plug) Stock Type Container/tray Time to Grow 3 months. Target Specifications Propagule Collection Instructions Propagule Processing/Propagule Characteristics Pre-Planting Propagule Treatments Seedlings with well-established roots Collect red fruits during mid-summer. Soak fruits in Dybvig seed cleaner and wash well. Strain seeds out with a screen. Seeds can be stored for up to 3 years. Seeds must be cold-moist stratified in media for a minimum of 85 days. A temperature of 21C is required for germination. Seeds will germinate in 2-3 weeks. None. Growing Area Preparation / Annual Practices for Perennial Crops Establishment Phase Details Keep seedlings indoors with moist soil. Length of Establishment Phase 3-4 weeks. Active Growth Phase Keep seedlings indoors with moist soil. Length of Active Growth Phase 12 weeks. Hardening Phase Length of Hardening Phase Harvesting, Storage and Shipping 3-4 weeks after germination, seedlings may be upplanted into pots. Store plugs in a shadehouse or indoors if it is not consistently warm outside. Length of Storage 4 months if up-planted into pots. Guidelines for Outplanting / Outplant only after winter has passed and outside soil Performance on Typical Sites is consistently warm. Plant 10 inches apart. Other Comments Fragaria vesca can be difficult to grow from seed. It is therefore recommended that vegetative propagation methods be used instead. INFORMATION SOURCES References The original 2006 protocol for Fragaria vesca is attached below. 1. USDA Plants Database. "Plants Profile for Fragaria Vesca (woodland Strawberry)." Web. 23 Apr. 2016.

2. Burke Museum. "Fragaria Vesca." WTU Herbarium Collection. Web. 23 Apr. 2016. 3. Luna, Tara. "Fragaria (vesca)." Native Plant Network. Web. 23 Apr. 2016. Seed propagation 4. Luna, Tara. "Fragaria (vesca)." Native Plant Network. Web. 23 Apr. 2016. Vegetative propagation (bareroot) 5. Young, Betty. "Fragaria (vesca)." Native Plant Network. Web. 23 Apr. 2016. Vegetative propagation (plugs) 6. USDA NRCS National Plant Data Center. "Wood Strawberry." Web. 23 Apr. 2016. 7. Hitchcock, C. Leo, and Arthur Cronquist. Flora of the Pacific Northwest; an Illustrated Manual. Seattle: U of Washington, 1973. Print. 8. Dumroese, R. Kasten., Tara Luna, and Thomas D. Landis. Nursery Manual for Native Plants: A Guide for Tribal Nurseries. Vol. 1. Washington, D.C.: U.S. Dept. of Agriculture, Forest Service, 2009. Print. Other Sources Consulted 1) Shulaev, Vladimir, et al. "The Genome of Woodland Strawberry (Fragaria Vesca)." Nature Genetics 43 (2010): 109-16. 26 Dec. 2010. Web. 23 Apr. 2016. 2) US Forest Service. "Fragaria Vesca." Web. 25 Apr. 2016. Protocol Author Kelly Ann Lee Date Protocol Created or Updated April 26, 2016

Plant Data Sheet Copyright 2005 Tim Hagan. All rights reserved. http://www.wnps.org/plants/fragaria_vesca.html http://runeberg.org/nordflor/pics/301.jpg Species: Fragaria vesca, woodland strawberry Range: North America and Europe (1) Climate, elevation: low to subalpine areas, south of 55 N (4) Local occurrence: Iron Goat trail, near Stevens Pass (1) Habitat preferences: Clearings or open forests (4) Plant strategy type/successional stage: low-growing perennial with long stolons (5) Associated species: Cedar/Hemlock forest margin(2) May be collected as: Seeds, stolons with plantlets, or divided plants (2) Collection restrictions or guidelines: Divide plants in spring or fall. Seed is collected when

the fruits turn red in mid summer. Seed can be processed by maceration of the fruit using a Dyb-vig cleaner, followed by washing and screening.(2) Seed germination: Seeds must be stratified for 85 days to break physiological dormancy. (2) Sow indoors 2 months before last frost date. Press into the soil firmly. Cover with paper to exclude light. Keep moist. Germination will occur in 2-3 weeks at an optimum soil temperature of 65-75 F. Up-pot at 3-4 weeks and transplant outside after the danger of frost has passed or direct seed outside as soon as the soil warms. (6) Seed life: 2 years (6) Recommended seed storage conditions: Unknown. Propagation recommendations: Seeds or cuttings Soil or medium requirements: Prefers a fertile, well-drained, moisture retentive soil(3) or moist sterile seeding mix (6) Installation form: Bare root or in 2 or 4 pots (2) Recommended planting density: 8-12 apart (6). Care requirements after installed: Water weekly at least in first year after installation (7). Normal rate of growth or spread;; lifespan: Establishes quickly, but individual plants can lose vigor after three years (3). Sources cited: 1. Washington Native Plant Society: http://www.wnps.org/plants/fragaria_vesca.html http://www.wnps.org/plant_lists/counties/king/documents/irongoattrail.doc 2. Native Plant Network: http://www.nativeplantnetwork.org/network/view.asp? protocol_id=212 3. Plants for a Future database: http://www.pfaf.org/database/plants.php? Fragaria+vesca+'Semperflorens' 4. Plants of the Pacific Northwest Coast: Washington, Oregon, British Columbia &

Alaska. Jim Pojar and Andy Mackinnon. (Vancouver, BC: Lone Pine Publishing, 1994) 5. borealforest.org: http://www.borealforest.org/herbs/herb14.htm 6. Territorial Seed Company: http://www.territorialseed.com/stores/1/strawberry_seed_culture_infor_w73c28.cfm? UserID=16105573&jsessionid=8c305d0d5e14$2B$23Lt 7. BackyardGardener.com: http://www.backyardgardener.com/plantname/pda_2e00.html Data compiled by Deborah Brown, May 7, 2006