NSave Nature to Survive

ISSN: 0974-0376 NSave Nature to Survive : Special issue, Vol. VI: 479-483: 2014 AN INTERNATIONAL QUARTERLY JOURNAL OF ENVIRONMENTAL SCIENCES www.theecoscan.in Studies on the effect of foliar application of nutrients and GA 3 on fruit yield and quality of winter season guava Brijesh Gaur et al., Keywords Borax Guava Total sugar Aacidity Fruit retention Proceedings of National Conference on Harmony with Nature in Context of Environmental Issues and Challenges of the 21 st Century (HARMONY - 2014) November 28-30, 2014, Udaipur, Rajasthan organized by Department of Environmental Sciences, Faculty of Earth Sciences, M. L. Sukhadia University, Udaipur - 313 001 (Rajasthan) in association with National Environmentalists Association, India www.neaindia.org 479

NSave Nature to Survive QUARTERLY Brijesh Gaur, Karma Beer, Tejraj Singh Hada*, Neeharika Kanth, M.M. Syamal Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi - 221 005, (U.P.), InDIA e-mail: banatshada0404@gmail.com Abstract The present investigation entitled studies on the effect of foliar application of nutrients and GA3 on fruit yield and quality of winter season guava was carried out in the Fruit Research Farm, Horticulture Unit, B.H.U., Varanasi, UP during the year 2010-2011. The experiment was laid out in Randomized Block Design with eleven treatment and replication thrice with a unit of one plant in each replication of a treatment and results shows that the maximum fruit retention (57.27%), minimum fruit drop (42.43), the maximum fruit length (6.07cm) width (5.92cm) and fruit weight (98.48g) were recorded with Borax (0.4%) application which was closely followed by higher concentration of Zinc sulphate (0.8%). Significantly maximum yield (48.63kg/tree) was recorded with Boron 0.4 per cent which was at par with Zinc sulphate 0.8 per cent, Boron 0.2 per cent and Potassium sulphate 2 per cent. Total soluble Solids (11.70 B) was maximum with minimum acidity (0.30%) and higher total sugar were recorded with foliar application of Borax 0.4 per cent along with highest ascorbic acid content of guava fruit. Foliar spray of GA3 50ppm caused 45.75 5% fruit set, 39.87% fruit retention. Hence, it can be concluded that foliar application of borax 0.4 per cent and GA3 50ppm can be recommended to guava grower to obtaining better yield and quality of winter season guava fruit. *Corresponding author INTRODUCTION Guava (Psidium guajava L.), the apple of the tropics, is one of the most popular fruits grown in tropical, sub-tropical and some parts of arid regions of India.Guava is an evergreen, shallow-rooted shrubs or small tree 9 m tall with spreading branches. The bark is smooth, mottled green or reddish brown and peels off in thin flakes to reveal the attractive bony aspect of its trunk. Fruits may be round, ovoid or pear-shaped, 2-4 inches long, and have 4 or 5 protruding floral remnants (sepals) at the apex. The fruit (Berry) is an excellent source of vitamin C (210-305mg / 100g fruit pulp) and pectin (0.5-1.8%) but has low energy (66cal /100g). The ripe fruits contain 12.3-26.3% dry matter, 77.9-86.9% moisture, 0.51-1.02% ash, 0.10-0.70% crude fat, 0.82-1.45% crude protein and 2.0-7.2% crude fiber. The fruit is also rich in minerals like Phosphorus (22.5-40.0mg / 100g pulp), Calcium (10.0-30.0mg / 100g pulp) and Iron (0.60-1.39mg / 100g pulp) as well as vitamins like Niacin (0.20-2.32mg / 100g pulp), Panthotenic acid, Thiamine (0.03-0.07mg / 100g pulp), Riboflavin (0.02-0.04 mg / 100g pulp) and vitamin A (Mitra and Bose, 2001). Yadav (2011) studied that with the spray of micronutrients and plant growth regulators the physical, chemical and yield parameters of the guava fruit were improved. In subtropical climate, there are three distinct periods of growth and fruiting. These three distinct periods are, Ambe bahar- February to March flowering and fruit ripens in July- August, Mrig bahar- June to July flowering and fruit ripens October to December and Hasta bahar- October to November flowering and fruit ripens in February to April (Shukla et al., 2008). The foliar application of nutrients and GA 3 play vital role in improving the quality and comparatively more effective for rapid recovery of plants under high ph condition. Most of micronutrients are rendered unavailable at high ph condition. The foliar feeding of fruit trees has gained much importance in recent years, as nutrient applied through soil are needed in higher quantities because some portion leaches down and some portion become unavailable to the plant due to soil complex reaction.thus keeping above facts in view the present investigation was undertaken with objectives susch as to see the influences of micronutrients and GA 3 on flowering and fruiting of guava fruits, to find out the effects of micronutrient and GA 3 on yield of guava fruits and to study the effects micronutrients and GA 3 on the quality of guava fruits. MATerials AND METHODS The present investigation entitled Studies on the effect of foliar application of nutrients and GA 3 on fruit yield and quality of winter season guava was carried out in the Fruit Research Farm, Horticulture Unit, B.H.U., Varanasi, U.P. during the year 2010-2011. Selection of variety Guava variety L-49 also known as sardar guava was selected for the present 480

EFFECT OF FOLIAR APPLICATION study. This variety was evolved through selection from open pollinated seedlings of Allahabad safeda at Pune (MH) and also known as Sardar guava. It is Semi dwarf 2.3 to 3.4 meter in height, heavy branching type with flat crown, leaves are large 12.8 to 13.2cm long, 6.8cm broad, elliptic-ovate to oblong in shape. Fruit is roundish ovate in shape, skin colour promise yellow with occasional red rot on the skin. The taste is good and keeping quality is excellent. The investigation was conducted on 6 years old guava plants planted at 6 6m apart under square system of planting. In order to assess the effects of various treatments, all the plants were subjected to uniform cultural practice during the period of experimentation. Experimental details The experiment was laid out in Randomized Block Design with three replications with a unit of one plant in each replication of a treatment Abbreviation Treatment Strength / Concentration of treatment T 1 Zinc sulphate 0.40% T 2 Zinc sulphate 0.80% T 3 Borax 0.20% T 4 Borax 0.40% T 5 Calcium Nitrate 0.40% T 6 Calcium Nitrate 0.80% T 7 GA 3 50ppm T 8 GA 3 100ppm T 9 Potassium sulphate 1.00% T 10 Potassium sulphate 2.00% T 11 Distilled water Control Where: Zinc sulphate (ZnSO 4 ), Borax (Na 2 B 4 O 7.10H 2 O), Calcium Nitrate (Ca (NO 3 ) 2 ), Potassium sulphate (K 2 SO 4 ) Preparation and Method of Application of Nutrient Solution The requirement of spray liquid for spraying a tree was assessed to be two liters. The quantity of micronutrient at the rate of 1g/L was used to make the solution concentration of 0.1 per cent. Similarly Zinc sulphate at the rate of 4g per liter and 8g /L make a solution of 0.4 per cent and 0.8 per cent, respectively. Observations on various characters of plant i.e. fruit set, fruit retention, fruit drop and yield worked out as per standard procedures. Observations recorded The observation were recorded on fruit retention (%), fruit drop (%), fruit length (cm) fruit width (cm) fruit weight (g), fruit yield (kg/tree), TSS, Acidity (%), Sugar (%) and ascorbic acid mg / 100g of pulp. Statistical analysis Panse and Sukhatme (1985) suggested the statistical analysis of the data obtained in the different set of experiment. The critical difference (C.D.) was calculated to assess the significance of difference between treatments and the results were found significant through F test. CD at 5 % level of significance was determined. RESULTS AND discussion Fruit retention and fruit drop (%) Fruit retention and fruit drop percentage was influenced significantly with different nutrients as compared to control. The maximum fruit retention (57.27%) and minimum fruit drop (42.43) was recorded by foliar application of Borax (0.4%) which was followed with lower concentration of Borax (0.2%) and ZnSO 4 (0.8%) and decreased in fruit drop. Borax response was more positive due to boron which play an important role in translocation of carbohydrates, auxin synthesis and increased pollen viability and fertilization. The maximum fruit drop was recorded under control. Similar result were also observed by Sinha et al. (1999) and Sarkar et al. (1984) in litchi, Singh and Vashistha (1997) is ber, Kundu and Mitra (1999) in guava Fruit Length, Width and Weight It has been observed that foliar application of different treatments proved beneficial in increasing the length, width and weight of Fruit in comparison to control. The maximum fruit length (6.07cm) width (5.92cm) and fruit weight (98.48g) were recorded with Borax (0.4%) T 4 which was closely followed by higher concentration of Zinc sulphate (0.8%) T 2. The minimum fruit length, width and weight were recorded under control. This increase in length and width of guava fruit may be that mineral nutrients (Boron and Zinc) appear to have direct role in hastening the process of cell division and cell elongation due to which size and weight would have improved. These results are in conformity with those reported by Arora and Singh (1972), Rajput and Chand (1976). They reported that boron spray increase fruit weight, length and diameter of guava fruit cv. Allahabad Safeda. Fruit yield kg tree -1 The fruit yield per plant was influenced by different treatment as compared to control. Significantly maximum yield (48.63 Kg tree -1 ) was recorded with Boron 0.4 per cent (T 4 ) which was at par with Zinc sulphate 0.8 per cent (T 2 ), Boron 0.2 per cent and Potassium sulphate 2 per cent. This was due to fact that borax spraying provides boron to the plant. It was believed that boron brings about inactivation of superfluous growth hormone by formation of complex compound. The importance of this element is improving the physiological activities of plant had been released but it is not clear whether it influenced directly or indirectly. These activities improve width length of fruit which ultimately increase the yield of fruit. These results are in close conformity with the findings of Brahmachari and Kumar (1997) in guava, Singh et al. (1993) in guava cv. L-49, Rath et al. (1980) in mango, Arora and Singh (1970) and Kundu and Mitra (1999) in guava. Total Soluble Solids (TSS B) The maximum total soluble Solids (11.70 B) were recorded with foliar application of Borax 0.4 per cent followed by Zinc 481

MALAY BHARTI AND RAM PRAVESH Table: Studies on the effect of foliar application of nutrient and GA 3 on fruit yield and quality parameters of winter season guava Treatment Fruit retention Fruit drop Fruit Fruit Fruit Yield (kg TSS Acidity Total Ascorbic acid (%) (%) length width weight tree -1 ) (%) Sugar (mg/100g of pulp ( B) (cm) (cm) (g) (%) T 1 : ZnSO4-0.4% 47.09 52.51 5.6 5.45 91.9 44.29 10.33 0.33 6.75 165 T 2 : ZnSO4-0.8% 52.82 47.18 5.96 5.66 94.6 47.5 11.46 0.31 7.05 170 T 3 : Borax- 0.2% 47.81 52.19 5.64 5.47 90.16 44.95 10.56 0.32 6.09 170 T 4 : Borax- 0.4% 57.27 42.33 6.07 5.92 98.48 48.63 11.7 0.3 7.51 172 T 5 : Ca (NO3)2-0.4% 41.46 58.54 5.45 5.23 82.3 42.17 9.68 0.37 6.59 156.4 T 6 : Ca(NO3)2-0.8% 46.54 53.46 5.51 5.36 87.68 43.87 9.92 0.36 6.65 158 T 7 : GA3-50 ppm 39.87 60.13 5.64 5.12 78.4 41.45 9.81 0.39 6.45 152.25 T 8 : GA3-100 ppm 42.26 57.74 5.65 5.17 80.79 42.56 9.5 0.38 6.54 154.38 T 9 : K2SO4-1% 46.94 53.06 5.62 5.37 88.6 43.25 9.88 0.35 6.85 165 T 10 : K2SO4-2% 50.56 49.44 5.77 5.32 92.6 46.14 10.65 0.31 6.93 168 T 11 : Control 36.37 63.33 5.15 5.02 69 35 8.5 0.48 6.1 149 SEm± 3.43 3.47 0.17 0.15 3.44 1.25 0.19 0.02 5.09 4.23 CD at 5 % 10.13 10.24 0.5 0.44 10.16 3.7 0.57 0.05 15.11 12.5 sulphate 0.8 per cent. Increase in total soluble solids might be that boron helps in transmembrane sugar transport, which may be the possible cause for improvement in boron sprayed trees. A notable characteristics of Borax is that it directly affect photosynthesis activity of plants (Lal and Patil, 1948). These result are in close conformity with Arora and Singh (1972) and Chaitanya et al. (1997) in guava, Singh and Vashistha (1997) in ber and Singh et al. (2004) in guava. Acidity (%) The present study indicates that acidity content guava fruit was significantly decreased by different treatment. Minimum acidity (0.30%) was found in Borax 0.4 per cent (T 4 ) followed by zinc sulphate 0.8 per cent while it was maximum (0.48%) in control (T 11 ). Acidity present was reduced with borax treated fruits which might be due to early ripening induced by this treatment during which degradation of acid might have occurred. It also appears that total soluble solids increased at the expense of acidity under these fruits. The acid under the influence of borax might have been fastly converted into sugars and their derivatives by the reaction involving the reversal of glycolytic path way or be used in respiration as both similarity. These results are close conformity with Rajput and Chand (1976); Ingle et al. (1993) and Sharma et al. (2013) in guava, Gohlani et al. (2012) and Mishra and Khan (1981) in litchi. Sugars (%) The maximum reducing sugar, non-reducing sugar and total sugar was recorded with foliar application of Borax 0.4 per cent followed by Zinc sulphate 0.8 per cent, while it was minimum under control. These results are in close conformity with findings of Stamper et al. (1999) and Rajput and Chand (1976) in guava fruits. Ascorbic acid (mg / 100 g of pulp) The maximum ascorbic acid 172.00 mg / 100 g of pulp was recorded with the higher concentration of Borax 0.4 per cent that was supported by Chandra et al. (1994). REFerenCE Arora, J.S. and Singh, J. R. 1972. Response of guava (Psidium guajava L.) to boron spray J. Jpn. 506. Hort. Sci. B. 3: 239-244. Arora, J.S. and Singh, J. R. 1970. Response of guava (Psidium guajava L.) to boron spray J. Jpn. 506. Hort. Sci. 3: 239-244. Brahmachari, V.S. and Kumar, R. 1997. Effect of foliar spray of mineral nutrients on fruit set, retention and cracking of litchi (Litchi chinensis) fruits. Haryana. J. Hort. Sci. 14(1-2): 1-3. Chaitanya, C.G., Kumar, G., Rana, B.L. and Muthew, A.K. 1997. Effect of foliar application of zinc and boron on yield and quality of guava cv. L-49. Haryana J. Hort. Sci. 26(1-2): 78-80. Chandra, R., Govind, S. and Basuchaudhari, P. 1994. Preharvest sprays of calcium nitrate and Alar on quality and post-harvest behaviour of guava fruits. India J. Hill Farming. 7(1): 51-56. Gohlani, S. and Bisen, B. P. 2012. Effect of different coating material on the storage behavior of custard apple (Annona squamosa L.). The Bioscan. 7(4): 637-640 Ingle, K.G., Khan, M.A.H. and Kshirsagar, R.E. 1993. Effect of foliar application of nutrients on yield and quality of guava (Psidium guajava L.) cv. L-49. P.K.V. Res. J. 17(1): 78-80. Kundu, S. and Mitra, S.K. 1999. Response of guava to foliar spray of copper, boron and zinc. Ind. Agric. 43(1-2): 49-54. Lal, K. N. and Patil, S. 1948. Role of boron in crop production. Science and Culture. 14(3-4): 87-90. Mishra, R.R. and Khan. I. 1981. Effect of 2.4, 5-T. and micronutrient on fruit size, cracking maturity and quality of litchi cv. Rose scented. Prog. Hort. 13(3-4): 87-90. Mitra, S. K. and Bose, T. K. 2001. Fruis: Tropical & Sub tropical VI. Naya Udyog, Kolkata, pp. 610-611. Rajput, C. B. S. and Chand, S. 1976. Effect of boron and zinc on the physico-chemical composition of guava fruits (Psidium guajava L.). J. Nat. Agric. Sci. Ceylon. 13: 49-54. Rath, S. S., Singh, R. L. Singh, B. and Singh, D. B. 1980. Effect of 482

EFFECT OF FOLIAR APPLICATION boron and zinc sprays on the physico-chemical composition of mango fruits. Punjab Hort. J. 20(1-2): 33-35. Sarkar, G. K., Sinha, M. M., Mishra, R. S. and Sriwastava, R. P. 1984. Effect of foliar application of mineral elements on cracking of litchi fruits. J. Haryana Hort. Sci. 13(1-2): 18-21. Sharma, A., Wali, V.K., Bakshi, P. and Jasrotia, A. 2013. Effect of organic and inorganic fertilizers on quality and shelf life of guava (psidium guajava l.) cv. Sardar. The Bioscan. 8(4): 1247-1250. Shukla, A. K., Kaushik, R. A., Pandey, D. and Sarolia, D. K. 2008. In: Guava. Published by Maharana Pratap University of Agriculture and technology, Udaipur. p.7. Singh, R. R., Joon, M. S. and Daulta, B. S. 1993. A not on the effect of urea and boric acid on physico-chemical composition of guava fruits cv. L-49. Haryana J. Hort. Sci. 12(12): 68-70. Singh, R.S. and Vashistha 1997. Effect of foliar spray of nutrients on fruit drop, yield and quality of ber cv. Seb. Haryana J. Hort. Sci. 26(1-2): 20-24. Singh, R., Chaturvedi, O. P. and Singh, R. 2004. Effect of pre-harvest spray of zinc, boron and calcium on the physico-chemical quality of guava fruit (Psidium guajava L.). International Seminar on Recent Trend on Hi-tech Hort and P.H.T. Kanpur, Feb. 4-6 2004, 204. Sinha, A. K., Singh, C. and Jain, B. P. 1999. Effect of plant growth substances and micronutrients on fruit set, fruit drop, fruit retention and creaking of litchi cv. Purvi. Indian J. Hort. 56(4): 309-311. Stampar, F., Hudina M. and Dolence, K. 1999. Influence of foliar fertilizer on yield and quality of apple (Mallus domestica Borkh.) Kumar Academic Publisher, pp. 91-94. Yadav, H. C., Yadav, A. L., Yadav, D. K. and Yadav, P. K. 2011. Effect of foliar application of micronutrients and GA3 on fruit yield and quality of rainy season guava. Plant Archives. 11(1): 147-149. 483

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