Řepka R., Veselá P. & Mráček J. (2014): Are there hybrids between Carex flacca and C. tomentosa

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Řepka R., Veselá P. & Mráček J. (2014): Are there hybrids between Carex flacca and C. tomentosa in the Czech Republic and Slovakia? Preslia 86: 367 379. Electronic Appendix 1. Comparison of morphological characters of plants preliminarily identified as C. danielis and their putative parental species. The origins of the studied populations are given in Table 1. The data on total variations of C. flacca and C. tomentosa were based on specimens from the whole of the Czech Republic (see Materials and methods for details). The characters stem length and leaf width were measured in the central part of the organs.

Character/population C. flacca 1a C. flacca 1b C. flacca 1c Number of studied plants 7 11 10 Rhizome creeping, shoots 40 75, strong creeping, shoots 40, strong creeping, shoots 20 30 Stem lenght* 33 69 cm 68 69 cm 39 56 cm Stem diameter 0.8 1.3 mm 1.15 1.25 mm 1.0 1.3 mm Leaves (l w)* 28 47 cm x 2.4 4.1 mm 28 47 x 2.2 3.4 mm 20 39 x 2.2 4.0 mm Leaf colouring bicoloured matt: on obverse greenish grey, bicoloured matt: on obverse greenish grey, bicoloured matt: on obverse greenish grey, Length of inflorescence 54 115 mm 77 99 mm 48 111 mm Length of inflorescence lower bract shorter, as as or slightly exceeding the inflorescence as as or exceeding inflorescence, 49 99 shorter than inflorescence, rarely as Male spike size (l w) 12 30 x 1.8 2.6 mm 29 40 x 2.0 2.5 mm 19 36 x 2.0 3.4 mm Male spike shape, colour narrow spindle clavate, brown purple brown narrow clavate strip like, brownish purple strip like narrowly spindle shaped, dark purple dull reddish brown Female spike number, position (1 )2, both upright and pendulous 2, both the upright and pendulous 2( 3), both the upright and pendulous Female spike size (l w) 20 31 x 4.3 6.0 mm 20 27 x 5.5 6.0 mm 9 29 x 4 6 mm Female spike length of peduncle 9 23( 40) mm 7 20 mm 2 36 mm

Female spike glume length usually exceeds the perigynium, rarely as as or shorter than it as as or slightly er then the perigynium slightly shorter than the perigynium or same length Perigynium shape and colour obovate, with 3 2 edges, green purple to dark purple obly obovate, obtusely triangular, black purple obovate ovate, 2 ribs indicated, inflated, purple with pale whitish eye or greenish with purple eye Perigynium size (l w) (2.3 )2.8 3.3 x (1.3 )1.5 1.7 mm (3.0 )3.2 3.6 x (1.3 )1.4 1.7( 1.8) mm 2.7 3.5 x 1.4 2.0 mm Perigynium hairs white hairs simple, erect, scattered only in the upper half, 0,1 or covered with papillae only 0.02 almost without papillae or only sparsely scattered flattened white hairs in the upper half, up to 0.1 translucent hairs covering the upper 1/3 to 2/3 of perigynium, sparsely n. densely arranged in all directions, from 0.05 to 0.1 Perigynium beak seeded, obtuse, truncate, as though twotoothed, from 0.1 to 0.2 seeded, obtuse, truncate, as though twotoothed, from 0.1 to 0.2 seeded, truncate, 0,1 0,3 Fertility perigynium with fully developed achenes perigynium with fully developed achenes perigynium with fully developed achenes

C. flacca 3 total variation of C. flacca C. " danielis" 1 C. " danielis" 2 1 93 6 4 creeping, shoots 20 70 creeping, shoots 20 150, with brown scales disintegrating into gross segments similar to C. flacca, but some plants resemble C. tomentosa identical with C. flacca, shoots 30 50 46 52 cm (25 )32 75( 90) 33 40( 53) 43 54 cm 1.15 1.2 mm 1.0 1.5 mm 0.5 1.5 mm 0.95 1.15 mm 14 26 cm x 3.8 4.3 mm (8 )18 52( 65) cm dl.. (2.0 )2.6 5.4( 6.5) mm šir 13 43 cm x 1.9 3.8 mm 37 48 cm x 2.5 4.2 mm bicoloured matt: on obverse greenish grey, bicoloured matt: on obverse greenish grey, on obverse greenish grey or green, on reverse side greenish grey or glaucous on obverse greyish green or glaucousgreenish, 95 101 mm (65 )73 147( 243) mm 45 122 mm 53 74 mm a little shorter than inflorescence, 80 89 shorter, as as or slightly exceeding the inflorescence, (56 )61 177( 205) shorter or as as inflorescence shorter than inflorescence 25 36 x 2 3 mm (8 )11 33( 50) mm x 2 4 mm 14 26 x 2.0 3.5 mm 20 28 x 2.0 2.5 mm strip like, dark purple to redish brown narrow spindle shaped to lanceolate, brownreddish brown narrow clavate shaped to narrow spindleshaped, dark purple to brownish purple narrow clavate shape to narrow lanceolate 2, both the upright and pendulous (1 )2 3( 4), pendulous at least for ripeness 1 2, upright and shortened 1 2, upright and shortened 33 36 x 3.5 4.0 mm (14 )19 50( 65) mm x 4.0 6.0( 8.5) mm 7 24 x 3.5 5.5 mm 16 22 x 5 6 mm 14 22 mm (7 )22 85( 260) mm 3 14 mm 5 10 mm

as as or er then the perigynium obovate, dark purple, with 2 green ribs 2.1 2.3 x 1.5 1.6 mm in the upper part of perigynium sparsely covered erect papillae 0.05 seeded, obtuse, truncate, 0,15 0,25 mm perigynium with fully developed achenes shorter, as as or slightly er than the perigynium oval to obovate, purple or light brown to gray brown, paler below the beak, matt, slightly inflated, with 2 significant lateral edges (2.3 )2.6 3.0( 3.2) mm x (1.3 )1.4 1.7( 2.0) mm in the upper half covered with papillae or very short hairs seeded, narrow, without teeths, 0,1 0,3 mm perigynium with fully developed achenes shorter, as as or slightly er than the perigynium flattened obovate to obly obovate, obtusely triangular variable in its length shorter, as or er than the perigynium obly obovate, biconvex, edges with light strips 2.5 3.1 x 1.4 1.8 mm 3.3 3.5 x 1.7 2.0 perigynium entirely glabrous or only in the upper half having highly dispersed tiny papillae or hairs to 0.5 without beak with a sketch of teeth or 0.1 to 0.3, beak with small teeth or no teeth fully developed or poorly developed achene, rarely missing very variable: some almost without papillae, some in the middle and upper parts with scattered short whitish papillae to 0.05 mm seeded and perigynium continuous narrowing into the beak, length 0.15 to 0.3 mm 2 plants with fully developed achenes, 1 plant with imperfectly developed achenes and 1 sterile plant

C. " danielis" 3 C. " danielis" 4 C. tomentosa 1a C. tomentosa 1b 1 3 3 7 shortly creeping, shoots 15 40 creeping, identicaly with C. flacca creeping, with thin shoots 35 90 creeping, with thin shoots 30 120 27 32 cm 40 50 cm 38 45 cm 29 58 cm 0.65 mm 1.2 1.3 mm 0.65 0.7 mm 0.6 0.8 mm 11 18 cm x 2.3 2.9 mm 36 44 cm x 4.1 4.5 mm 25 40 cm x 1.3 1.8 mm 16 40 ( 61) cm x 2.0 2.08 mm bicoloured, matt: on obverse greenish grey, bicoloured, matt: on obverse greenish grey, green, matt dark green to greyish green, matt 37 40 mm 70 112 mm 32 37 mm 33 49 mm shorter than inflorescence, 28 30 shorter than or slightly exceeding inflorescence always shorter than inflorescence always shorter than inflorescence, 21 43 15 19 x 1.2 1.7 mm 14 20 x 2.2 mm 17 23 x 1.9 2.0 mm 16 25 x 2.1 3.0 mm lanceolate, brownish purple clavate shaped to lanceolate, light brown strip shaped to narrow lanceolate, brownish purple clavate shaped to stripe shaped, purple/greenish 2, upright with short stalk 2 3, upright with short stalk 1 2, upright and sessile 1 2, upright and almost sessile 10 11 x 4.4 4.7 mm 10 15 x 5.5 6.0 mm 9 11 x 4.2 5.0 mm 8 16 x 3.5 5.0 mm 3 9 mm 3 11( 28) mm 1.5 2.5 mm 3.0 5.0 mm

shorter than the perigynium or the same length at most shorter than the perigynium or the same length at most significantly shorter than the perigynium, go back to 1/2 to 2/3 of their length shorter than the perigynium or the same length at most obovate, 3 sideways, green, purple based obovate, with 3 2 edges, pale and deep brownish purple, based on a lighter, whitish to greenish background broad obovate, oval in diameter, yellowish, later grey obovate, obtusely triangular, greenish grey, later pale brownish 2.2 2.4 x 1.2 1.3 mm 2.8 2.9 x 1.7 2.0 mm 1.9 2.5 x 1.4 1.8 mm 1.8 2.1 x 1.2 1.4 mm covered the entire surface of the papillae ca 0.01 to 0.02 densely covered with whitish hairs with enlarged bases (most in its upper half), up to 0.05 the entire surface thickly covered with translucent simple curved upward or straight hairs, from 0.08 to 0.15 over the entire surface covered densely whitish papillae 0.05, or rarely by hairs to 0.15 seeded, truncate, with 2 small blunt teeth, low, green, 0.15 to 0.2 seeded, truncate, toothless or two small low obtuse teeth, dark purple to darker, from 0.1 to 0.2 seeded, broadly conical, obtuse 0.05 to 0.1 broad, with 2 triangular teeth, 0.05 to 0.2, or almost absent perigynium with maturing achenes (cannot decide) perigynium mostly sterile, some form achenes, but do not mature, dries perigynium with fully developed achenes perigynium with fully developed achenes

C. tomentosa 1c C. tomentosa 2 total variation of C. tomentosa 8 1 40 horizontaly creeping with shoots 60 120 mm creeping with shoots 40 113 with, creeping shoots, covered with dark purple matte or shiny not disintegrating scales 28 46 cm 17 24 (12 )17 62( 85) 0.6 0.7 mm 04 0.6 mm 0.5 0.8( 1.0) mm 20 35 cm x 2.2 2.7 mm 9 14 cm x 1.5 1.9 mm (13 )17 53( 65) cm x (1.6 )2.0 3.8( 4.5) mm dark green to greyish green, matt dark green to slightly greyish green bright green to dark green, matt 24 35 mm 16 26 mm (14 )20 58( 90) mm always shorter than inflorescence always shorter than inflorescence, 15 20 mm always shorter than inflorescence, (1 )7 46( 70) 13 17 x 1.8 3.0 mm 12 x 2.3 mm (10 )13 28( 36) mm x 2 3 mm clavate shaped to narrow spindle shaped, later stripe shaped, light brown to light redishbrown narrow clavate shaped, dark purple cylindrical to clavate shaped, purple to dark redishbrown, rare light brown 1 2, vertically or obliquely upright, almost sessile 2, upright, sessile 1 2( 3), vertically or obliquely upright, almost sessile or with short stalk 7 11 x 3.5 4.0 mm 6 x 3.5 4.0 mm 6 25 x 4.0 6.5 mm 1.5 4.0 mm 1 3 mm 3 8 mm

always shorter than the perigynium (about one third) obovate with three blunt edges, brownish above, the rest of the body pale grey greenish significantly shorter than the perigynium, go back to 1/2 to 2/3 of their length obovate, with obtusely lateral ribs, pale greyish green shorter than the perigynium or the same length at most, 2.3 3.0 obovate, with two prominent lateral ribs, pale greenish gray to brown later 1.9 2.4 x 1.3 1.6 mm 2.2 2.3 x 1.4 1.5 mm (2.1 )2.3 2.5 mm x (1.3 )1.5 1.8( 2.0) mm whitish translucent, slightly xiphoid, down broad, flattened, directed in all directions, densely arranged in all directions, in the top third most dense missing or with wide triangular 2 teeth, 0.1 to 0.3 across densely covered with yellowish white 0.1 and the hairs directed in all directions seeded, wide, with wide short dark purplecolored teeth, 0.15 densely covered with 0.1, white to greyish, up pointing hairs seeded, wide, from 0.05 to 0.15, wide and sharp teeth perigynium with fully developed achenes perigynium with fully developed achenes perigynium with fully developed achenes