Breeding PD Resistant Winegrapes Andy Walker, Alan Tenscher, Summaira Riaz, Cecilia Agüero, Rong Hu, Nina Romero Funding from CDFA PD/GWSS Board, AVF, and the Louis P. Martini and Louise Rossi Endowed Chair Funds
Pierce s Disease
PD Resistance Breeding Lenoir (Jacquez, Black Spanish) a V. aestivalis x V. vinifera hybrid 100s of years of breeding hindered by multigenic resistance Blanc du Bois We discovered single dominant gene for resistance in V. arizonica (b43-17) b43-17 was collected by Olmo in Monterrey Mexico and we stumbled upon it!
Walker Grape Breeding Program Olmo gave me seeds from 12 populations of V. rupestris x M. rotundifolia Tested for resistance to phylloxera, dagger nematode, root-knot nematode, PD Plants had small amounts of tomentum on internodes and petioles Strong resistance, but odd ratios of resistant to susceptible
rupestris x rotundifolia 2002 - began mapping these resistances in sibling matings first with RAPD and AFLP markers and then in 2006 with SSR markers First discovery they were not rupestris x rotundifolia! Used DNA markers to fingerprint all possible pollen sources rupestris x Mexican Vitis spp. AJEV (2007) 58:494-498
PD Resistance of Olmo s Mexican Collections 8R,0I,0S 5R 4I 1S 4R,2I,1S 9R 7I 8S
First grape collecting lesson leaves of three, let it be
Southwest Vitis germplasm
V.#girdiana# V.#arizonica# V.#berlandieri,#V.#cinerea# V.#mustangensis# V.#riparia,#V.#acerifolia# V.#rupestris# Hybrid' Claire Heinitz
Mapping and Characterizing PD Resistance Summaira Riaz V. arizonica/candicans b43-17 has single dominant gene for resistance to PD and it s homozygous All progeny from crosses to b43-17 are resistant to PD Genetically mapped PD resistance (PdR1), to chromosome 14. Linked markers have been used for marker-assisted selection (MAS)
5.95 8.22 3.96 4.75 3.40 11.87 3.33 2.81 5.54 0.42 3.67 2.80 1.38 4.96 0.98 3.40 0.97 0.38 0.14 3.69 3.18 0.21 0.29 0.34 1.64 1.04 8.25 F8909-17 9621 SCU15 VMCNg1e1 UDV050 VMC9c1 VVIO32 VMC1e12 VVC34 VMC9f4-1 VVIP22 VrZAG112 VMC6c10 VMC5b3 UDV33 VMC2a5 VVIV69 VMCNg2b7.2 VMCNg3h8 VVCh14-29 VVCh14-70 PdR1a A010 VVCh14-56 UDV95 VVIN64 ctg1025882 VVIS70 VVIP26 VMC6e1 VMCNg1g1.1 UDV025 ctg1010193 0.98 3.40 0.97 0.38 0.14 3.69 VMCNg2b7.2 VMCNg3h8, VVCh14-29 VVCh14-70 PdR1a VVCh14-56. A010 UDV95, VVIN64 6.22 3.15 1.74 7.41 0.42 5.01 7.07 4.36 3.99 3.38 4.78 3.47 1.09 4.62 4.31 0.26 3.66 0.23 0.39 0.45 6.12 0.77 0.53 0.03 0.81 0.54 2.40 0.98 1.33 F8909-08 04190 population VMCNg1e1 VVC62 VVIP05 VVIQ32 UDV050 VMC9c1 VMC1e12 VVC34 VVIP22 VrZAG112 VMC6c10 VMC5b3 UDV033 VMC2a5 VVIV69 ctg1026876 VMCNg2b7.2 VVCh14-27 VVCh14-28 VVCh14-29 VVCh14-30 VVCh14-70 PdR1b VVCh14-02 VVCh14-10 VVCh14-09 UDV95 UDV025 ctg1025882 VVIS70 VVIP26 VMC6e1 VMCNg1g1.1 VVIN94 ctg101093 VVIN70 Krivanek et al. 2005. Theor Appl Genet 111:110-119 Riaz et al. 2006. Theor Appl Genet 113:1317-1329 0.26 3.66 0.23 0.39 0.45 6.12 VMCNg2b7.2, VVCh14-27 VVCh14-30 VVCh14-28 VVCh14-29, VVCh14-70 PdR1b VVCh14-02 VVCh14-10 UDV095, VVCh14-09 UDV025
Testing PdR1 Cecilia Agüero New gene constructs were prepared with grape promoters, and under testing.
Marker-Assisted Selection for PdR1 DNA extracted from seedlings Aggressive growing techniques to get flowers and fruit in year 2 Two year cycle with marker-assisted selection (MAS) Select for lack of symptoms and low bacterial levels F1 = 50% vinifera; BC1 = 75%; BC2 = 88%; BC3 = 94%; BC4 = 97% Optimizes classical breeding not GMOs
Breeding Objectives Develop large seedling populations at the 97% vinifera level in diverse, high quality vinifera winegrape backgrounds Intercross advanced high quality selections with Xf resistance from other resistance sources Use and map multigenic resistances V. arizonica/girdiana b42-26 and others Characterize additional unique resistances to make broadly and durably resistant varieties
Proven Potential of Classical Breeding F8909-08 to 97% vinifera in about 12 yrs From peppery, herbaceous wines with blue-purple pigments to high quality vinifera characters
Field Testing PD Resistant Selections Wines have been made compared with wine from classic vinifera cultivars made at the same small scale 75%, 88%, 94% and 97% vinifera at Beringer/Yountville along the Napa River have been inoculated multiple times Small scale wines have been made since 2010 with Davis and Napa fruit 88% and 94% in Fredericksburg TX, Auburn AL (88%), and Gainesville, FL (94%). 2014 new plots in Temecula, Napa (2X), Texas and Alabama
Napa PdR1b (94% vinifera) vs pure vinifera
07355-075 along Napa River
62.5% Cab Sauv, 12.5% Carig, 12.5% Chard 09338-016 Not yet in large scale field trials Late bloom, midseason ripening Small berries, small clusters Medium productivity
62.5% Cab Sauv, 12.5% Carig, 12.5% Chard 09314-102 Temecula, Sonoma 75, Silverado Early bloom, early ripening Small - medium berries, medium large clusters High productivity
50% Zin, 25% Petite Sirah, 12.5% Cab Sauv 09331-047 Caymus 1125, Temecula, Silverado Late bloom, mid-season ripening Relatively large berries, large clusters Moderate-low productivity
50% Petite Sirah, 25% Cab Sauv 07355-075 Caymus 375, Sonoma, Early bloom, early ripening Relatively large berries, medium large clusters Medium productivity
50% Sylvaner, 12.5% Cabernet Sauvignon, Carignane, Chardonnay 09356-235 Not yet in large scale field trials Mid-season bloom and ripening Large berries, loose medium clusters High productivity
Stacking PD Resistance Lines 2006 & 2008 crossed PdR1a x PdR1b no decrease in mean Xf levels. 2011 crossed 97% vinifera PdR1b x 75% vinifera b42-26 lines to create 86% vinifera 2014 crossed 97% vinifera PdR1b x 88% vinifera b42-26 line to create ~ 92% vinifera Added PdR2 b40-14 from Chihuahua V. arizonica and many other sources
2017 and Beyond Broaden the V. vinifera background acidity, color, tannins, aromatics, ripening profiles Add Powdery Mildew from multiple sources and advanced backcross generations
New Powdery Mildew Resistance Loci Ren2 V. cinerea x V. rupestris Ren3 American spp. (`Regent`) Ren5 Run1 Run2 M. rotundifolia Ren1 V. sylvestris/vinifera Ren4 V. romanetii Ren6 Ren7 V. piazeskii Gene stacking Co-evolving pathogen (?) Different mechanisms (?) Host-adapted PM strains (Musc4) Interactions of these if combined (?)
UC DAVIS VITICULTURE AND ENOLOGY White Winemaking Small lots 15-300 lbs: Goal to express fruit flavors using a reductive style Fruit harvested 22 Brix; juice TA ~8 g/l and wine ~7 g/l; YAN 250ppm to 350 ppm; Generous use of dry ice; 50 ppm SO 2 at crush; Yeast: QA23 Fermentation temp: 52 F; No MLF; DO measured at all steps Racked with 35 ppm SO 2 added; Cold stabilized; Bottled in December using screwcaps with tin liners
UC DAVIS VITICULTURE AND ENOLOGY Red Winemaking Maximize color and balance tannin extraction Fruit harvested 24 to 26 Brix; must ~7.5 g/l and wine ~5.8 g/l.; 25 ppm SO 2 at crush; acid and nutrient additions as necessary Yeast: EC1118; co-inoculation with Viniflora Oenos for MLF; fermentation temp: 85 to 72 F Small lots made within larger research fermentor (TJ s); limited temp control; punched down twice a day; larger lots fermented in TJ s, pump-over 3 times a day; Pressed at dryness (<2 g/l of sugar); Racked, 35 ppm SO 2 added; Bottled in December using screwcaps with Saranex liners
Thanks!
09338-016 Highly resistant to Pierce s disease. Wines from Davis fruit only, well ranked. Small berries, small compact clusters; mod to late bloom, ripens mid-season; moderate productivity. Comments on wine: light straw-gold color, apple-melon, lychee, floral aromas, pineapple, green apple, juicy, well-balanced; Sauvignon blanc like
09314-102 Highly resistant to PD. Early bloom and the fruit ripens early; has small to medium berries and relatively large clusters; it is highly productive. Reminiscent of Sauvignon blanc to some others more like Chardonnay Tasting comments have included: light straw to clear color, citrus, lime, tropical, gooseberry golden delicious apple flavors; bright fruit, slightly bitter.
09331-047 Highly resistant to PD; commercial scale wines have been made Blooms relatively late, but ripens midseason; berries are medium, clusters are well-filled and relatively large. More productive with cane pruning Highly ranked from Davis and Napa fruit. Tasting comments include: medium dark red purple; berry pie, cassis, black olive, herbal, dried hay, coffee, vegetal like Cabernet Sauvignon, moderate tannins, soft finish.
07355-075 Wines with characteristics of both Cabernet Sauvignon and Petite Sirah. Commercial scale wines have been made from Napa. Early budbreak, bloom, and ripening. Berries are relatively large and the wellfilled clusters are medium in size. Highly resistant to PD Only 94% to be released; tasting notes include: dark-red purple color, bright red fruit, raspberry, cherry, ripe, tannic, elegant.