This document contains presentations to be made at the fifty-second session of the Technical Working Party for Vegetables (TWV), as follows:

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International Union for the Protection of New Varieties of Plants E Technical Working Party for Vegetables Fifty-Second Session Beijing, China, September 17 to 21, 2018 Original: English Date: September 4, 2018 EXPERIENCES WITH NEW TYPES AND SPECIES Document prepared by the Office of the Union Disclaimer: this document does not represent UPOV policies or guidance This document contains presentations to be made at the fifty-second session of the Technical Working Party for Vegetables (TWV), as follows: - Annex I: Presentation on Solanum sisymbriifollium Lam. by an expert from France - Annex II: Presentation on Solanum torvum Sw. by an expert from France - Annex III: Presentation on a new type of Kale in Japan by an expert from Japan [Annexes follow]

ANNEX I GEVES DUS Expériences NEW Species Solanum sisymbriifollium Lam. is commonly known as Sticky Nightshade, the Fire and Ice plant, Litchi Tomato, or Morelle de Balbis Presentation at the TWV 52 September 2018 Some general information 1/2 included in http://eol.org/pages/581198/details https://en.wikipedia.org/wiki/solanum_sisymbriifolium Plant: annual, up to 1.5 m tall. covered of sticky hairs that are barely visible to the naked eye and give the plant its common name of viscid nightshade or sticky nightshade. Leaves : highly dissected and both sides are covered with sharp prickles. These are sometimes called spines or thorns, but technically they are prickles as they are outgrowths of the epidermis and not modified branches (spines). Inflorescence : in cyme of five to ten flowers, yellow stamens protruding Flowers: large (diameter of about 5 cm), white, sometimes tinged with purple, with 5 yellow anthers held in the centre. Fruits: enclosed by a prickly calyx, until they are ripe, when they turn bright red and the calyx peels back to expose the fruit to birds and other animals which eat them and disperse the seeds. A edible red and globose berry at maturity. It grows in clusters of 10 to 12 units and looks like cherry tomato but its sweet sour taste is between sour cherry, lychee and tomato. It contains many seeds about 2 mm in diameter often resembling naturally.

Annex I, page 2 Some general information 1/2 included in http://eol.org/pages/581198/details https://en.wikipedia.org/wiki/solanum_sisymbriifolium Possible use a trap crop or companion crop to protect potatoes from potato cyst nematode. The stems and leaves contain solasodine (poisonous alkaloid chemical compound which makes the plant very resistant to many pests and diseases. With the exception of potato beetles and tomato worms. It can also be used as a hedge plant to keep animals out of a garden, because it is covered with prickles. GEVES Experiences on the matter Definition of a National protocol in 2018 To finalize DUS test for National listing (then UE listing). - sowing: mi- April - planting: end of May Clearly inspired by characteristics of the Eggplant TG/ 117/4 Close to the S. torvum National protocol [Annex II follows]

ANNEX II GEVES DUS Expériences NEW Species Solanum torvum Sw. also known as turkey berry, prickly nightshade, shoo shoo bush, wild eggplant, pea eggplant, pea aubergine and many Saisissez other names votre texte Presentation at the TWV 52 September 2018 Some general information 1/2 included in https://en.wikipedia.org/wiki/solanum_torvum a bushy, erect and spiny perennial plant used horticulturally as a rootstock for eggplant. The plant: usually 2 or 3 m in height and 2 cm in basal diameter, Saisissez but votre may texte reach 5m in height and 8 cm in basal diameter. The shrub usually: a single stem at ground level, but it may branch on the lower stem. The stem bark is gray and nearly smooth with raised lenticels. The inner bark has a green layer over an ivory color. The plants growing on firm soil, have weak taproots and well-developed laterals. The roots are white. Foliage is confined to the growing twigs. The twigs are gray-green and covered with star-shaped hairs. The spines are short and slightly curved and vary from thick throughout the plant, including the leaf midrib, to entirely absent. The leaves are opposite or one per node, broadly ovate with the border entire or deeply lobed. The petioles are 1 to 6 cm long and the blades are 7 to 23 by 5 to 18 cm and covered with short hairs.

Annex II, page 2 Some general information 2/2 included in https://en.wikipedia.org/wiki/solanum_torvum The flowers are white, tubular with 5 pointed lobes, and grouped in corymbiform cymes. They are shed soon after opening. The fruits are berries that grow in clusters of tiny green spheres (ca. 1 cm in diameter) that look like green peas. They become yellow when fully Saisissez ripe. They votre texte are thin-fleshed and contain numerous flat, round, brown seeds. Reproduction Flowering and fruiting: continuous after the shrubs reach about 1 to 1.5 m in height. The seedlings are common in recently disturbed ground. Frugivorous birds eat the fruits and spread the seeds. It can be propagated vegetatively by placing branch cuttings, with or without leaves, in a mist chamber for one month. Hybrids crossed with eggplant in an attempt to incorporate genes for resistance to Verticillium wilt into the vegetable. GEVES Experiences on the matter Definition of a National protocol in 2012 DUS test for National listing (then UE listing). - sowing: mi-march - planting: end of May Clearly inspired by some characteristics of the Eggplant TG/ 117/4 [Annex III follows]]

Annex III New type of Kale in Japan and study about Kale Takayuki NISHIKAWA Plant Variety Protection Office, Intellectual Property Division, Ministry of Agriculture, Forestry and Fisheries (MAFF) Japan New type of Kale Variety in Japan Denomination: PURPLE VARIE Registration number: 26981 Special characteristic: Variegation of leaf Process of Breeding : Mutant of Curry Kale Scarlet Usage: Ornamental Propagation method: vegetative propagation 1

Annex III, page 2 New type of Kale Variety in Japan Useful characteristics for describe PURPLE VARIE 1 Variegation of leaf 2 Incision of margin(development of lobes) of leaf Purple Varie Kitchen Red Kitchen form UPOV TG 3 2 Study about Kale 1. DUS examination for Kale in Japan 2. Basic Information about Kale 3. Possibility to expand the application range of current UPOV TG for Kale 4. Useful new characteristics for current UPOV TG for Kale 5. Interspecific/intergeneric hybrid including Kale in Japan 4 3

Annex III, page 3 DUS examination for Kale in Japan Grouping for examination Botanical name Brassica oleracea L. var. acephala DC. is used for Kale in Japan. We divide Kale to 2 groups as Habotan(Traditional Ornamental Kale) and Kale(Vegetable Kale) for DUS examination. Habotan(Traditional Ornamental Kale) National TG had been developed in 1981. 27 varieties had been registered till the end of 2017. Kale(Vegetable Kale) National TG had been developed in 2004 based on UPOV TG. 6 varieties had been registered till the end of 2017. 4 DUS examination for Kale in Japan The application range of the National TG for Kale includes all variants in below table. UPOV code Botanical name (USDA_GRIN) common name(usda_grin) BRASS_OLE_ALB Brassica oleracea var. alboglabra Chinese broccoli, Chinese kale BRASS_OLE_COS BRASS_OLE_GAM BRASS_OLE_GAR BRASS_OLE_GAS BRASS_OLE_GAV Brassica oleracea L. var. costata DC. Brassica oleracea L. convar. acephala (DC.) Alef. var. medullosa Thell. Brassica oleracea L. var. medullosa Thell. Brassica oleracea L. var. ramosa DC. Brassica oleracea L. convar. acephala (DC.) Alef. var. sabellica L. Brassica oleracea L. var. sabellica L. Brassica oleracea L. convar. acephala (DC.) Alef. var. viridis L. Brassica oleracea L. var. viridis L. Bedford cabbage, braganza, Portuguese cabbage, Portuguese tree kale, seakale cabbage, Tronchuda cabbage; Tronchuda kale Marrow-stem Kale branching bush kale, branching cabbage, bush kale, perennial kale, perpetual kale, thousand-head kale borecole; curled kitchen kale, curly kale, Scotch kale, ornamental cabbage, ornamental kale Collards; Cow cabbage; Fodder Kale; Kale; Portuguese kale; Spring-heading cabbage, Tree kale Brassica oleracea L. var. palmifolia DC. giant Jersey kale, Jersey kale, palm kale, palm-tree kale, 6 tree kale 5

Annex III, page 4 DUS examination for Kale in Japan This table shows the number of Kale varieties which had examined and are examining in Japan. Botanical name is checked by the parent varieties. Botanical name number of registration number of under variety examination variety Brassica oleracea L. var. sabellica L. 1 1 Brassica oleracea L. var. viridis L. 0 3 Brassica oleracea L. var. costata DC x viridis L. Brassica oleracea L. var. palmifolia DC x viridis L. 2 0 1 0 Brassica oleracea L. var. sabellica L. x viridis L. 0 1 (Brassica oleracea L. var. costata DC x viridis L.) x var. palmifolia DC 1 0 details unkown 1 0 6 Basic cultivation way of examination for Kale at the Unzen station Type of cultivation: open field DUS examination for Kale in Japan Cultivation period: August to November (4 months) Sowing: Sowing to tray (128 cells) at the end of July Transplanting: Transplanting to 7.5cm pot about 10days after sowing Planting: Planting at the beginning of September about 30days after sowing Number of plant: seed propagation variety: 32, vegetative propagation variety: 17 Spacing: 1row, interval:60cm, bed width:100cm Observation: Observation at the beginning of November, when the plants grow enough for harvest and leaf color is still green. 7

Annex III, page 5 Basic Information about Kale The range of Kale is very wide Life cycle: annual~perennial Height: 0.3~4m Flowering: 1st year ~ 3rd year Main Use part: leaf 9 http://treecollards.blogspot.com/ 8 GRIN Taxonomy for Brassica oleracea L. and rape kale Botanical name Brassica oleracea var. alboglabra Brassica oleracea var. botrytis Brassica oleracea var. capitata Brassica oleracea L. var. costata DC. Brassica oleracea var. gemmifera Brassica oleracea var. gongylodes Brassica oleracea var. italica Brassica oleracea var. medullosa Brassica oleracea var. oleracea Brassica oleracea var. palmifolia Brassica oleracea var. ramosa Brassica oleracea var. sabauda Brassica oleracea var. sabellica Commonname Chinese broccoli, Chinese kale cauliflower cabbage, red cabbage, white cabbage Bedford cabbage, braganza, Portuguese cabbage, Portuguese tree kale,seakale cabbage, Tronchuda cabbage; Tronchuda kale Brussels sprouts cabbage turnip, kohlrabi, asparagus broccoli, broccoli, sprouting broccoli marrow-stem kale wild cabbage giant Jersey kale, Jersey kale, palm kale, palm-tree kale, tree kale branching bush kale, branching cabbage, bush kale, perennial kale, perpetual kale, thousand-head kale Savoy cabbage borecole; curled kitchen kale, curly kale, Scotch kale,ornamental cabbage, ornamental kale Brassica oleracea var. viridis Collards; Cow cabbage; Fodder Kale; Kale; Portuguese kale; Spring-heading cabbage, Tree kale Brassica napus L. subsp. napus var. pabularia (DC.) Hanover-salad, rape kale, Siberian kale Alef. 10 9

Annex III, page 6 Possibility to expand the application range of current UPOV TG for Kale It would be difficult to make a common TG for Kale because the range of Kale is very wide. But if it is possible to set limited cultivation for DUS examination it is possible to cover wider range of kale by one TG. We never cultivate tomato for over one year for DUS examination. 11 http://blog.ecorinvillage.com/staffblog/2014/11/ 10 Possibility to expand the application range of current UPOV TG for Kale 12 11

Annex III, page 7 Possibility to expand the application range of current UPOV TG for Kale Merit to promote international harmonization for kale to make it easy to choice TG for unknown variant varieties for intervariant hybrid varieties Demerit Difficult to make TG (we need long time and much information) Completeness lowers and the needs to revise becomes higher If we agree with above demerit and limited cultivation approach, I would like to make a draft TG for kale. 13 12 Possibility to expand the application range of current UPOV TG for Kale Our experience of examination for Kale Brassica oleracea L. var. sabellica L.: 34 registration varieties (include NL:24) Brassica oleracea L. var. viridis L.: 5 registration varieties (GB) Brassica oleracea L. var. medullosa Thell. 5 registration varieties(include DE:3) Data from Pluto database 14 13

Annex III, page 8 Possible range to discuss to apply the TG for kale based on our experience UPOV code Botanical name (USDA_GRIN) common name(usda_grin) common name(upov_genie) BRASS_OLE_ALB Brassica oleracea var. alboglabra Chinese broccoli, Chinese kale Chinese broccoli; Chinese kale BRASS_OLE_COS Brassica oleracea L. var. costata DC. Bedford cabbage, braganza, Portuguese cabbage, Portuguese tree kale,seakale cabbage, Tronchuda cabbage; Tronchuda kale Bedford cabbage; Braganza; Portugese cole; Portuguese kale; Seakale cabbage; Tronchuda cabbage; Tronchuda kale BRASS_OLE_GAM Brassica oleracea L. convar. acephala (DC.) Alef. var. medullosa Thell. Brassica oleracea L. var. medullosa Thell. Marrow-stem Kale Marrow-stem Kale BRASS_OLE_GAR Brassica oleracea L. var. ramosa DC. branching bush kale, branching cabbage, bush kale, perennial kale, perpetual kale, thousand-head kale branching bush kale; branching cabbage; perennial kale; perpetual kale; thousand-head kale BRASS_OLE_GAS Brassica oleracea L. convar. acephala (DC.) Alef. var. sabellica L. Brassica oleracea L. var. sabellica L. borecole; curled kitchen kale, curly kale, Scotch kale, ornamental cabbage, ornamental kale Borecole; Curly Kale; Dwarf Siberian kale; Kitchen kale; Scotch kale BRASS_OLE_GAV Brassica oleracea L. convar. acephala (DC.) Alef. var. viridis L. Brassica oleracea L. var. viridis L. Collards; Cow cabbage; Fodder Collards; Cow cabbage; Fodder Kale; Kale; Kale; Kale; Spring-heading cabbage; Portuguese kale; Spring-heading cabbage, Tall kale; Tree kale Tree kale Brassica oleracea L. var. palmifolia DC. giant Jersey kale, Jersey kale, palm kale, palm-tree kale, tree kale 15 BRASS_NAP_PAB Brassica napus L. subsp. napus var. pabularia (DC.) Alef. Hanover-salad, rape kale, Siberian kale Hanover-salad, rape kale, Siberian kale 14 Useful new characteristics for current UPOV TG for Kale At least following characteristics are necessary to be added to current TG for Curry Kale. 1. Variegation of leaf for assessment of PURPLE VARIE 2. Incision of margin(development of lobes) of leaf 16 Purple Varie Kitchen Red form UPOV TG 15

Annex III, page 9 Interspecific/intergeneric hybrid including Kale in Japan Brussels sprouts Habotan TG: Habotan Broccoli Kale TG: Broccoli for DUS examination Daikon Kale TG: (Pending) for Commercial Brussels sprouts Kale TG: Brussels sprouts Kale Wild rocket TG: Kale provisional Cabbage Kale TG: Kale 16 Thank you for your attention [End of Annex III and of document]