MUSSELING UP MATT MILLER NZ FATS AND OILS NOV 2016
RE-VALUING THE MUSSEL WITH HVN The aim to increase the value of Greenshell Mussel (GSM) based food export products. This will be achieved by determining the effect of these GSM products (and bioactives within) on improving joint and bone health while reducing inflammation Cawthron Institute and Sanford Limited business led research Through 4 objectives, we aim to develop the evidence dossier to make a general health claim for GSM food products We will deliver at least 4 new, high-value functional food products for export
GREENSHELL MUSSEL INDUSTRY EXPORTS 2015 Value (NZD$ - FOB) 2015 Volume (Kg) Price ($/KG) Greenshell Mussels $256.7m 27,877,370 ~$8-9 Mussel Oil $32.7m 10,958 >$3000 Freeze Dried Mussel Powder $12.1m 255,192 ~$50
SCIENCE PLAN Objective 1: To characterise known and novel active components of GSM using new high-throughput analytical capability. Objective 2: To demonstrate the efficacy of GSM foods/ingredients using in vivo and in vitro models of inflammation, joint and bone health.
SCIENCE PLAN Objective 3: To develop targeted consumer-focused functional formulated foods containing GSM ingredients and to develop an anti-inflammatory activity index to assess the efficacy for GSM food products. Objective 4: To determine the comparative nutrikinetics including the bioavailability of orally administered specific active ingredients from whole mussels, mussel extracts and novel functional foods in humans.
NIR NEAR INFRARED SPECTROSCOPY
COMPOSITIONAL ANALYSIS OF GSM Proximate analysis (i.e. fat, protein, glycogen, moisture) Provides insight into: physiological status or condition quality (sensory properties, consumer acceptance) Further lipid analysis: EPA, DHA, other fatty acids Applied to studies (to mussels) on: reproductive conditioning physiology and nutrition ecology genetics sensory analysis (taste, market acceptance)
PROXIMATE ANALYSIS (I.E. PROTEIN, FAT, GLYCOGEN, MOISTURE PLUS OILS) Standard (chemical) lab techniques: Food lab Well established methods Precise and reproducible However Costly ($500 per sample) Slow turnaround (eg. week) Rapid NIR analytical techniques: Fast (10 seconds), cheap, high throughput Method development ($$) Doesn t need precision of lab methods Can determine Fat, protein, glycogen, moisture, ash? EPA? DHA?
INTRODUCTION TO NIRS NIR absorption: associated with molecular vibration NIR (700 to 2500 nm) NIR absorption of cookie dough
MODEL DEVELOPMENT (CALIBRATION) Predicted Spectral data + Chemical data Absorbance Units 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Sample code % Fat % Protein % Carbs % Moisture A 1.4 13.8 2.8 80.3 B 1.9 7.9 3.0 83.7 C 2.5 12.1 4.0 76.5 D 4.7 12.5 1.2 80.6..etc..etc.etc.etc.etc 12000 11000 10000 9000 8000 7000 Wavenumber cm-1 6000 5000 4000 Mussels_HVN-001_F_20160419.0 Mussels_HVN-002_F_20160419.0 Mussels_HVN-003_F_20160419.0 Mussels_HVN-004_M_20160419.0 Mussels_HVN-005_M_20160419.0 Mussels_HVN-006_M_20160419.0 Mussels_HVN-007_F_20160419.0 Mussels_HVN-008_F_20160419.0 Mussels_HVN-009_F_20160419.0 Mussels_HVN-010_M_20160419.0 Mussels_HVN-011_M_20160419.0 Mussels_HVN-012_M_20160419.0 Mussels_HVN-013_MIX_20160419.0 Mussels_HVN-014_MIX_20160419.0 Mussels_HVN-015_MIX_20160419.0 Mussels_HVN-016_MIX_20160419.0 Mussels_HVN-017_MIX_20160419.0 Mussels_HVN-018_MIX_20160419.0 Mussels_HVN-019_MIX_20160419.0 Mussels_HVN-020_MIX_20160419.0 Mussels_HVN-021_MIX_20160419.0 Mussels_HVN-022_MIX_20160419.0 Mussels_HVN-023_MIX_20160419.0 Mussels_HVN-024_MIX_20160419.0 Mussels_HVN-025_MIX_20160419.0 Mussels_HVN-026_MIX_20160419.0 Mussels_HVN-027_MIX_20160419.0 Mussels_HVN-028_MIX_20160419.0 Mussels_HVN-029_MIX_20160419.0 Mussels_HVN-030_MIX_20160419.0 Mussels_HVN-031_MIX_20160419.0 Mussels_HVN-032_MIX_20160419.0 Mussels_HVN-033_MIX_20160419.0 Mussels_HVN-034_MIX_20160419.0 Mussels_HVN-035_MIX_20160419.0 Mussels_HVN-036_MIX_20160419.0 Mussels_HVN-037_MIX_20160419.0 Mussels_HVN-038_MIX_20160419.0 Mussels_HVN-039_MIX_20160419.0 Mussels_HVN-040_MIX_20160419.0 Mussels_HVN-041_MIX_20160419.0 Mussels_HVN-042_MIX_20160419.0 Mussels_HVN-043_MIX_20160419.0 Mussels_HVN-044_MIX_20160419.0 Mussels_HVN-045_F_20160518.0 Mussels_HVN-046_F_20160518.0 Mussels_HVN-047_F_20160518.0 Mussels_HVN-048_M_20160518.0 Mussels_HVN-049_M_20160518.0 Mussels_HVN-050_M_20160518.0 Mussels_HVN-051_F_20160518.0 Mussels_HVN-052_F_20160518.0 Mussels_HVN-053_F_20160518.0 Mussels_HVN-054_M_20160518.0 Mussels_HVN-055_M_20160518.0 Mussels_HVN-056_M_20160518.0 Mussels_HVN-057_F_20160616.0 Mussels_HVN-058_F_20160616.0 Mussels_HVN-059_F_20160616.0 Mussels_HVN-060_M_20160616.0 Mussels_HVN-061_M_20160616.0 Mussels_HVN-062_M_20160616.0 Mussels_HVN-063_F_20160616.0 Mussels_HVN-064_F_20160616.0 Mussels_HVN-065_F_20160616.0 Mussels_HVN-066_M_20160616.0 Mussels_HVN-067_M_20160616.0 Mussels_HVN-068_M_20160616.0 Mussels_HVN-069_F_20160719.0 Mussels_HVN-070_F_20160719.0 Mussels_HVN-071_F_20160719.0 Mussels_HVN-072_M_20160719.0 C:\Users\Mattm\Desktop\Temp folder\calibration validation\raw Data Files\HVN Mussels_HVN-073_M_20160719.0 Mussels_HVN-074_M_20160719.0 Mussels_HVN-075_F_20160719.0 Mussels_HVN-076_F_20160719.0 Mussels_HVN-077_F_20160719.0 Mussels_HVN-078_M_20160719.0 Mussels_HVN-079_M_20160719.0 Mussels_HVN-080_M_20160719.0 Page 1/1 HVN-001;F;;HVN HVN-002;F;;HVN HVN-003;F;;HVN HVN-007;F;;HVN HVN-008;F;;HVN HVN-004;M;;HVN HVN-009;F;;HVN HVN-005;M;;HVN HVN-006;M;;HVN HVN-010;M;;HVN HVN-011;M;;HVN HVN-012;M;;HVN HVN-013;MIX;;HVN HVN-014;MIX;;HVN HVN-015;MIX;;HVN HVN-016;MIX;;HVN HVN-017;MIX;;HVN Mussels HVN-018;MIX;;HVN HVN-019;MIX;;HVN HVN-020;MIX;;HVN HVN-021;MIX;;HVN HVN-022;MIX;;HVN HVN-023;MIX;;HVN HVN-024;MIX;;HVN HVN-025;MIX;;HVN HVN-026;MIX;;HVN HVN-027;MIX;;HVN HVN-028;MIX;;HVN HVN-029;MIX;;HVN HVN-030;MIX;;HVN HVN-031;MIX;;HVN HVN-032;MIX;;HVN HVN-033;MIX;;HVN HVN-034;MIX;;HVN HVN-035;MIX;;HVN HVN-036;MIX;;HVN HVN-037;MIX;;HVN HVN-038;MIX;;HVN HVN-039;MIX;;HVN HVN-040;MIX;;HVN HVN-041;MIX;;HVN HVN-042;MIX;;HVN HVN-043;MIX;;HVN HVN-044;MIX;;HVN HNV-045;F;;HVN HVN-046;F;;HVN HVN-047;F;;HVN HVN-048;M;;HVN HVN-049;M;;HVN HVN-050;M;;HVN HVN-051;F;;HVN Mus 19/04/2016 HVN-052;F;;HVN HVN-053;F;;HVN HVN-054;M;;HVN HVN-055;M;;HVN HVN-056;M;;HVN 057;F;;HVN 058;F;;HVN 059;F;;HVN 060;M;;HVN 061;M;;HVN 062;M;;HVN 063;F;;HVN Mussels 18/05/2016 064;F;;HVN 065;F;;HVN 066;M;;HVN 067;M;;HVN 068;M;;HVN 069;F;;HVN 070;F;;HVN 071;F;;HVN 072;M;;HVN 073;M;;HVN 074;M;;HVN 075;F;;HVN Mussels 076;F;;HVN 077;F;;HVN 078;M;;HVN 079;M;;HVN Mussel HVN - 080;M;;HVN 16/06/2016 19/07/2016 Optimising the model : - define scan region - mathematical processing - define # of factors - outlier removal Chemometric modelling Actual Develop model
80 GSM MUSSEL SAMPLE SPECTRA 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 12000 11000 10000 9000 8000 Wavenumber cm-1 7000 6000 5000 4000 Mussels_HVN-001_F_20160419.0 Mussels_HVN-002_F_20160419.0 Mussels_HVN-003_F_20160419.0 Mussels_HVN-004_M_20160419.0 Mussels_HVN-005_M_20160419.0 Mussels_HVN-006_M_20160419.0 Mussels_HVN-007_F_20160419.0 Mussels_HVN-008_F_20160419.0 Mussels_HVN-009_F_20160419.0 Mussels_HVN-010_M_20160419.0 Mussels_HVN-011_M_20160419.0 Mussels_HVN-012_M_20160419.0 Mussels_HVN-013_MIX_20160419.0 Mussels_HVN-014_MIX_20160419.0 Mussels_HVN-015_MIX_20160419.0 Mussels_HVN-016_MIX_20160419.0 Mussels_HVN-017_MIX_20160419.0 Mussels_HVN-018_MIX_20160419.0 Mussels_HVN-019_MIX_20160419.0 Mussels_HVN-020_MIX_20160419.0 Mussels_HVN-021_MIX_20160419.0 Mussels_HVN-022_MIX_20160419.0 Mussels_HVN-023_MIX_20160419.0 Mussels_HVN-024_MIX_20160419.0 Mussels_HVN-025_MIX_20160419.0 Mussels_HVN-026_MIX_20160419.0 Mussels_HVN-027_MIX_20160419.0 Mussels_HVN-028_MIX_20160419.0 Mussels_HVN-029_MIX_20160419.0 Mussels_HVN-030_MIX_20160419.0 Mussels_HVN-031_MIX_20160419.0 Mussels_HVN-032_MIX_20160419.0 Mussels_HVN-033_MIX_20160419.0 Mussels_HVN-034_MIX_20160419.0 Mussels_HVN-035_MIX_20160419.0 Mussels_HVN-036_MIX_20160419.0 Mussels_HVN-037_MIX_20160419.0 Mussels_HVN-038_MIX_20160419.0 Mussels_HVN-039_MIX_20160419.0 Mussels_HVN-040_MIX_20160419.0 Mussels_HVN-041_MIX_20160419.0 Mussels_HVN-042_MIX_20160419.0 Mussels_HVN-043_MIX_20160419.0 Mussels_HVN-044_MIX_20160419.0 Mussels_HVN-045_F_20160518.0 Mussels_HVN-046_F_20160518.0 Mussels_HVN-047_F_20160518.0 Mussels_HVN-048_M_20160518.0 Mussels_HVN-049_M_20160518.0 Mussels_HVN-050_M_20160518.0 Mussels_HVN-051_F_20160518.0 Mussels_HVN-052_F_20160518.0 Mussels_HVN-053_F_20160518.0 Mussels_HVN-054_M_20160518.0 Mussels_HVN-055_M_20160518.0 C:\Users\Mattm\Desktop\Temp folder\calibration validation\raw Data Files\HVN Mussels_HVN-056_M_20160518.0 Mussels_HVN-057_F_20160616.0 Mussels_HVN-058_F_20160616.0 Mussels_HVN-059_F_20160616.0 Mussels_HVN-060_M_20160616.0 Mussels_HVN-061_M_20160616.0 Mussels_HVN-062_M_20160616.0 Mussels_HVN-063_F_20160616.0 HVN-001;F;;HVN HVN-002;F;;HVN HVN-003;F;;HVN HVN-007;F;;HVN HVN-008;F;;HVN HVN-004;M;;HVN HVN-009;F;;HVN HVN-005;M;;HVN HVN-006;M;;HVN HVN-010;M;;HVN HVN-011;M;;HVN HVN-012;M;;HVN HVN-013;MIX;;HVN HVN-014;MIX;;HVN HVN-015;MIX;;HVN HVN-016;MIX;;HVN HVN-017;MIX;;HVN Mussels HVN-018;MIX;;HVN HVN-019;MIX;;HVN HVN-020;MIX;;HVN HVN-021;MIX;;HVN HVN-022;MIX;;HVN HVN-023;MIX;;HVN HVN-024;MIX;;HVN HVN-025;MIX;;HVN HVN-026;MIX;;HVN HVN-027;MIX;;HVN HVN-028;MIX;;HVN HVN-029;MIX;;HVN HVN-030;MIX;;HVN HVN-031;MIX;;HVN HVN-032;MIX;;HVN HVN-033;MIX;;HVN HVN-034;MIX;;HVN HVN-035;MIX;;HVN HVN-036;MIX;;HVN HVN-037;MIX;;HVN HVN-038;MIX;;HVN HVN-039;MIX;;HVN HVN-040;MIX;;HVN HVN-041;MIX;;HVN HVN-042;MIX;;HVN HVN-043;MIX;;HVN HVN-044;MIX;;HVN HNV-045;F;;HVN HVN-046;F;;HVN HVN-047;F;;HVN HVN-048;M;;HVN HVN-049;M;;HVN HVN-050;M;;HVN HVN-051;F;;HVN Mus 19/04/2016 HVN-052;F;;HVN HVN-053;F;;HVN HVN-054;M;;HVN HVN-055;M;;HVN HVN-056;M;;HVN 057;F;;HVN 058;F;;HVN 059;F;;HVN 060;M;;HVN 061;M;;HVN 062;M;;HVN HVN 063;F;;HVN Mussels Mussels 18/05/2016 16/06/2016
104 GSM MUSSEL PROVISIONAL RESULTS
80 GSM MUSSEL PROVISIONAL RESULTS
g/100g RESULTS - MUSSEL COMPOSITION g/100g Female Site 1 Male Site 1 April May June July August September October April May June July August September October
mg/100g RESULTS - OMEGA 3 CONTENT mg/100g Female Site 1 Male Site 1 April May June July August September October April May June July August September October
MUSSELING UP PROJECT Still early days (8 months in to a 3 year program) In vitro and In vivo trials (Just starting) Clinical trial (starting late 2017) Novel food products for export markets
10 TH AAOCS BIANNUAL MEETING BIOTECHNOLOGY, LIPIDOMICS AND NUTRITION CHÂTEAU TANUNDA, BAROSSA VALLEY SA DATE 11-13 TH SEPTEMBER 2017 FOR MORE INFORMATION CONTACT: MATT.MILLER@CAWTHRON.ORG.NZ