Monk fruit (luo han guo) Siraitia grosvenorii. By:! Ye Ren! Catherine Tao Yang! Biyu Lei! YukSin Lo! Rachel Kim!

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Monk fruit (luo han guo) Siraitia grosvenorii By:! Ye Ren! Catherine Tao Yang! Biyu Lei! YukSin Lo! Rachel Kim!

General ideas!! Originally from south China!! Kingdom Plantae!! Order Cucurbitales!! Family Cucurbitaceae!

Appearance!! Either oval or spherical, 4.5-8.5cm long, 3.5-6cm diameter!! Surface brown, green-brown, has dark patches and yellow pubescent, and some contain 6 to 11 vertical lines!! Leaves alternate, petiole length 2-7cm!! Light body, crisp, thin skin, and easy to break!! Fruit flesh (endocarp) spongy, light brown.!! Seed is flat round, around 1.5cm long. 1.2cm width!

Environment for growth!! Cool temperature environment!! Huge temperature difference between day and night!! Humidity!! Need large amount of water due to the branches and leaves!! Like sunlight, but can not suffer strong sunlight, 7-8 hours illumination is enough for its growth!! Soil does not really matter, but prefer humus soil!

Chemical component!! Mogroside V (main component)!! Fructose!! Amino acid!! Flavone!! Vitamin C!! Linoleic acid, palmitic acid, stearic acid, palmitoliec acid, myristic acid, lauric acid in seed!

Advantages of Mogroside V!! High sweet degree. Approximate 250-400 times of sucrose!! Low calories!! Fine light color and fine water soluble!! Good stability. In addition, it can be maintained at a ph value 2.0~10.0 for two years without quality affected!! Safe and dependable. No side effects found during thousands of years of use by people in China!! All natural source!

Application of Mogrosides!! Mogrosides can partially or wholly replace sucrose in the food industry!! Mogrosides (Luo Han Guo Extract) can be blended with some other herbal extracts (Green Tea, Kuduz, etc) to make teas and herbal drinks with possible health benefits!! Mogrosides use in the dairy industry to develop a new product line!! Sugar-Substitute for coffee and tea!

Where? Southern part of China Guilin city, Guangxi

Who? Ø Ø Ø Ø Joint Venture company; partnership with Tate & Lyle Vertically integrated value chain from beg. to end Location: Waikato Innovation Center (NZ) Works with Chinese government

Growing of Monk Fruit! Seedlings are taken from mother plants! DI water cleans seedlings! Sodium hypochlorite or silver chloride disinfect! In next 40-50 days, sprouts placed on agar medium to promote growth of callus tissue! New agar medium for fostering of differentiated plants for another 200-240 days! Moved to another agar medium to promote roots development for 25-35 days! Put in pot with potting mix for 30-40 days

Conditions for growth! Subtropical climate zone of hilly, mountainous areas! Direct plants towards the sun at a 200-600m altitude! Warm, moist, and foggy climate (short-day sunshine)! Difference in day to night temperature

Pest control! Pyrenthreum pesticide is used! Extracted from the Chrysanthemum family of permethrin, fenvalerate, tetramethrin, allethrin, cypermethrin etc.! Enriched with phosphate and potassium throughout the growing cycle! Pesticide residual on monk fruit??

Time March & April: seedlings transferred to the fields April & May: organic pesticide used for pest control August-November: bloom and harvest

Harvest Fruit is picked before ripening Fruit is stored dried to maintain supply of fruit year-round and allow transport of the fruit to distant markets

After Harvest! Harvested plants go under inspection before they are processed.! The excess, if any, are stored in a controlled atmosphere of cool storage from 0-5 deg. Celsius

PROCESSING!

HOW THIS WHOLE FRUIT EXTRACT IS OBTAINED?

PROCESS! 1. SELECT! 2. CLEANING! 3. CRACKING OPEN! Select Stabilizing Separation 4. WHOLE FRUIT EXTRACTION! 5. ENZYME DIGESTION! Cleaning Enzyme digestion Filtration 6. STABILIZING! Cracking Whole fruit extraction Final extracts 7. SEPARATION! 8. FILTRATION!

SELECT! Only pick the fresh and ripe fruits, but not the green ones! Discard decay fruits!

CLEANING! Clean in a moving water bath! Two water bath compartments: Initial and further washing! Help to remove all the dirt! Water should not contain disinfectant or chlorine!

CRACKING OPEN! The outer peel of the fruit is cracked open! Extraction is based on peel and pulp, and not the seed! It is important not to break the seeds!

WHOLE FRUIT EXTRACTION! Boiled in water for 1-2 hours! This increases its sweetness and make the color of the extracts lighter! Water extract components from the fruit! No chemicals!

ENZYME DIGESTION! Contain pectin which are not stable during storage! Pectinase is conditioned (1 hrs, 45C)! ph is adjusted by citric acid solution.! Final product < 5! Pectinase is added to extracted liquids! Fast mixed for 5mins!

STABILIZATION! Contains active pectinase & possibly enzymes and bacteria! Contaminants negatively affect the stability & its shelf life! Remove contaminants by boiling for about 0.5-1 hour!!

SEPARATION! The extracted solid is separated through a draining process! Drains off liquid from the container!

Filtration! Processed by high speed centrifugation! All extracted liquid is combined! Filtered by different mesh! Made into dry powders! Bacterial growth is prevented during the process! Powder

Output-Delight, Cost, and Convenience Rachel Kim

Delight! Superior taste-profile compared to Stevia! Less bitter after-taste! Used to sweeten products like yogurt, juice, desserts, cereals! A major monk-fruit sweetener, Nectresse

Consumer Marketing! Key Method: Increasing awareness and familiarity with the fruit itself.! Focusing on Monk-fruit sweetener as fruit-derived.

! Cost of box of 40 singleserve packets of Monk Fruit In the Raw is $3.49.! More expensive than Stevia Cost

! Convenient Product Monk Fruit to Go by Nevella Company Convenience

Kashi Cereal and Granola Bear Naked Granola

So Delicious Coconut ice cream and Vitalicious Products

Conclusion! Monk fruit is a safe, natural way of sweetening products with out adding sugar or calories.! It is projected to become a new product that will enter the American market and be a major player in the lowcalorie sweetener industry.! Video