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Pulse crop variety update January 30, 31 February 1, 2 2017 Bunyamin Tar an, Bert Vandenberg, Tom Warkentin, Sabine Banniza, Kirstin Bett Goals in Pulse Breeding Increase yield and stability Grower satisfaction/lower production cost disease resistance, lodging resistance, herbicide tolerance, etc Consumer satisfaction colour, size, appearance, nutrition, reasonable cost Rapid introduction of new technologies and ideas that allow us to increase/maintain genetic AND economic gains 1

LENTIL VARIETIES LENTIL and FABA BEAN Breeding Program Changes All new varieties have PBR applied for status since 2014. All lentils will have imidazolinone tolerance from now on. All white-flowered faba bean with low vicine/convicine from 2017 onward 2

LENTIL BREEDING RESEARCH Main limitations: Greenhouse, growth room and seed handling facilities appropriate for scope and scale of breeding objectives Canada produces 50% of global lentils. - More expectations - More problems - IYOP increase demand for seed overseas 2016 LARGE GREEN (% ACREAGE IN SK, TOTAL 708,000 ACRES) 2016 LENTIL OTHERS (% ACREAGE IN SK, TOTAL 290,000 ACRES) PLATO CDC 2 IMPRESS CL CDC 2 LAIRD IMPROVE CDC 3 8 PERIDOT CDC CL 2 GREENSTAR CDC IMPOWER CDC CL 11 18 ESTON VICEROY CDC 8 9 Not reported GREENLAND CDC 21 35 Not reported IMVINCIBLE CDC CL 18 56 2016 RED LENTIL (% ACREAGE in SK; TOTAL: 2.43M ACRES) IMPACT CDC PROCLAIM CDC CRIMSON KING 1 1 1 1 IMAX CL CDC 5 DAZIL CDC 22 Not reported 24 MAXIM CDC 41 Data Courtesy of SCIC (2016) 3

Market class Variety Newer Red Lentils Herbi cide Toler ance Years Tested * Yield % CDC Maxim CL Area 1 & 2 Area 3 & 4 Maturity Rating Resistance to: Ascochyta Blight Anthra cnose Race 1 Seed Weight (g/1000) Small red CDC Maxim CL 10 100 100 E/M G G 40 CDC Dazil CL 9 104 98 E/M G F 35 CDC Impulse CL 7 108 95 E/M G G 44 CDC Proclaim CL 6 105 101 E/M G G 40 CDC Redmoon 6 115 102 E/M G G 41 Extra small red CDC Roxy 6 103 102 E/M G G 32 * Co-op and Regional Trials in Saskatchewan since 2006. Comparisons to the check variety, small red lentil CDC Maxim CL. CL indicates Clearfield variety. Maturity ratings: Normal maturity range in days based on May 1 seeding is E = 100, VL = 110 but maturity can be much earlier in dry years, much later in cool wet years. See Page 2 for more information on maturity range in lentil. LENTIL PERFORMANCE 2015 vs 2016 2015 super dry 2016 super wet Need to take this into account, more prudent to use 3 year or longer data! 4

CDC Maxim CDC Redmoon Year of release: 2015 Market Class: Small Red (Conventional type) Variety name: (Registration Test ID: 3646-4) (CDC Redmoon) Yield advantage: 113 % of CDC Maxim ( 37 sites) 2015 8 sites 112% of CDC Maxim; 2016-8 sites, 110% of CDC Maxim still looking good. Maturity rating: early to medium Disease ratings: Good for ascochyta and anthracnose Quality attributes: similar to CDC Maxim (40 g), seed a bit more plump Other agronomic traits: similar to CDC Maxim Year of release: 2014 CDC Maxim CDC Proclaim Market Class: Small Red ( Clearfield type) Variety name: (Registration Test ID: IBC 550) CDC Proclaim Yield advantage: 105% of Maxim ( 47 sites 5 years, consistent across years) In 2015 8 sites, 103% of CDC Maxim; 2016-8 sites, 108% of CDC Maxim Maturity rating: early to medium Disease ratings: Similar to CDC Maxim Quality attributes: seed size 40g same as CDC Maxim 5

Year of release: 2014 CDC Impulse CDC Maxim Market Class: Small Red (Clearfield type) Variety name: (Registration Test ID: IBC 479) CDC Impulse Yield advantage: 108 % of CDC Maxim ( 44 site-year total) In 2015, 110% of CDC Maxim; 2016-8 sites, 99% of CDC Maxim Disease ratings: Similar to CDC Maxim Quality attributes: seed size (44 g) 10% bigger than CDC Maxim Other agronomic traits: slightly taller than Maxim Year of release: 2014 CDC Roxy CDC Maxim Extra Small Red (Conventional type) Variety name: (Registration Test ID: 3959-6 ) CDC Roxy Yield advantage: 104% of CDC Maxim ( 45 site-year) In 2015 108% of CDC Maxim over 8 sites; 2016-8 sites, 97% of CDC Maxim Quality attributes: 31 mg seed size, similar to CDC Rosetown Disease ratings: Good for ascochyta and anthracnose Maturity rating: early to medium Other agronomic traits: a little later to flower than CDC Maxim 6

Market class Variety Newest Green Lentils.. Herbi cide Toler ance Years Tested * Yield % CDC Maxim CL Area 1 & 2 Area 3 & 4 Height (cm) Maturi ty Rating Resistance to: Ascoch Anthra yta cnose Blight Race 1 Seed Weight (g/1000) Small green CDC Imvincible CL 10 96 83 33 E G G 34 CDC Kermit 7 106 102 36 E/M G G 34 Extra small green CDC Asterix 9 99 101 30 E G F 26 Medium CDC Impress green CL 6 87 71 34 M G P 52 CDC Imigreen CL 7 78 71 44 M G VP 57 Large green CDC Greenland 7 89 70 38 M/L G VP 64 CDC Greenstar 8 101 78 40 M/L G F 73 CDC Impower CL 9 85 68 41 M/L G VP 64 All have green seed coat and yellow cotyledons CDC Viceroy CDC Kermit Year of release: 2014 Market Class: Small Green (Conventional type) Variety name: (Registration Test ID: 3592-13) CDC Kermit Yield advantage: 106% of CDC Maxim ( 37 site-year) (2015 8 sites 107% of CDC Maxim); 2016-8 sites, 98% of CDC Maxim Maturity rating: early to medium Disease ratings: Good for ascochyta and anthracnose Quality attributes: 33 g similar to CDC Imvincible Other agronomic traits: similar to CDC Imvincible, bit taller? 7

Crop Kind: Lentil Market Class: Small Red (Conventional type) Variety name: (Registration Test ID: 3674-15 ) CDC??? Yield advantage: 111% of CDC Maxim ( 52 sites) CONSISTENT! Maturity rating: early to medium Review of 2016 releases Disease ratings: Similar to CDC Maxim Quality attributes: seed size similar to CDC Maxim Other agronomic traits: a little later to flower than CDC Maxim Year of PGDC Recommendation: 2013 2014 - PBR applied for LENTIL PERFORMANCE (wet vs dry years) 3674-15 small red Yield No. of Years % Maxim Sites 2011-16 111 % 52 2016 111 % 8 8

Crop Kind: Lentil Market Class: Small Red ( Clearfield type) Variety name: (Registration Test ID: IBC 975) CDC??? Yield advantage: 97% of Maxim ( 31 sites 3 years) NOT CONSISTENT Maturity rating: early to medium Disease ratings: Similar to CDC Maxim Review of 2016 releases Quality attributes: seed size same as CDC Maxim Year of Recommendation: February 2016 PGDC Year of Release:?? 2016 PBR applied for RECOMMENDATION TO DROP IT LENTIL PERFORMANCE (wet years) IBC 975 small red (CL) Yield No. of Years % Maxim Sites 2014 111 % 18 2015 97 % 31 2016 72 % 8 Inconsistent in high water stress year Better to drop it...better ones coming 9

Crop Kind: Lentil Market Class: Large Green (Clearfield type) Variety name: (Registration Test ID: IBC 937 (CDC????) Yield advantage: 88 % of CDC Maxim (46 sites) Best Clearfield large green to date Maturity rating: same as large Disease ratings: good for ascochyta Quality attributes: seed size (75 g) okay for large greens Other agronomic traits: slightly taller than Viceroy Year of Registration/Release: Recommended at PGDC Feb 2016 PBR applied for 2016 LENTIL PERFORMANCE IBC 937 Large green Yield No. of Years % Maxim Sites 2013-16 88 % 46 2016 72 % 8 10

Crop Kind: Lentil Market Class: Green Cotyledon (Clearfield type) Variety name: (Registration Test ID: IBC 839 (CDC????) Yield advantage: 91 % of CDC Maxim (46 sites) Best green cotyledon type to date Maturity rating: same as small reds Disease ratings: good for ascochyta Quality attributes: seed size 35g Other agronomic traits: slightly taller than Maxim Year of Registration/Release: Recommended at PGDC Feb 2016 PBR applied for 2016 LENTIL PERFORMANCE IBC 839 Green Cotyledon type Yield No. of Years % Maxim Sites 2013-16 91 % 46 2016 78 % 8 11

CHICKPEA VARIETIES Current cultivars NAME TYPE Long term yield % Amit Asco. Blight (0-9 scale) Seed weight (g/1000 seeds) 2015 Days to maturity Amit K 100 4.5 258 110 CDC Consul D 112 (9) 4.0 303 110 CDC Orion K 108 (10) 5.1 434 109 CDC Leader K 109 (11) 4.5 390 107 ` CDC Palmer K 109 (6) 4.9 418 108 Percent acreage in SK (Source SCIC) 2014 2015 2016 CDC Orion 39 52 35 CDC Leader 4 31 43 CDC Frontier 41 5 8 CDC Palmer 0 0 1 Amit 13 6 11 CDC Luna 2 0 0 12

CDC Palmer CDC Consul CDC Palmer Kabuli Chickpea High yielding medium-large seeded kabuli. Predominantly 9-10 mm seed size similar to CDC Orion. Earlier maturing than CDC Orion. Moderately resistant to ascochyta blight similar to CDC Orion. CDC Consul Desi Chickpea Desi cultivar that combines high yield, improved ascochyta blight resistance, and good seed quality. Plump, medium seed size with a light tan seed coat colour suitable for whole, dehulled/split seed markets and milling. Medium maturity. Main goals chickpea breeding program : Competitive advantage for farmers: Yield (x seed size) x price Yield stability Reduced risk and minimized production cost: ascochyta blight resistance; early maturity; frost tolerance Ease of management: herbicide tolerance; growth habit/plant stature 13

FABA BEAN VARIETIES FABA BEAN BREEDING Green manure types White flower (extraction) Large seeded coloured flower (traditional market) ALL varieties need to be low vicine ALL seed producers should exceed CSGA isolation requirements?? Outcrossing concerns. 14

Tannins: associated with flower colour Seed Growers- make sure seed crops are isolated - faba bean is partially outcrossing. Tannins (polyphenolics) Concentrated in seed hulls Eliminated by either of 2 genes zt1 and zt2 Co-expression of white flower trait Seed coats of coloured flower type: 4 to 8 % tannin Seed coat of white flower type: 0 to 0.6 % tannin better digestibility of protein and a higher proportion of available energy Zero tannin 310 mg Normal tannin 700 mg CDC Released Faba bean Variety Years Tested* Yield % CDC Fatima COLOURED FLOWER (NORMAL TANNIN) Maturity Lodging ¹ (1-9) Rating (days) Seed Weight (g/1000) CDC Fatima 11 100 3.8 105 520 FB9-4 9 92 3.7 104 680 CDC SSNS-1 10 91 3.4 105 335 186S-11 6 106 3.1 106 749 247-13 4 107 3.4 106 620 WHITE FLOWER (ZERO TANNIN) CDC Snowdrop 8 94 2.6 104 335 ¹ Lodging score (1-9) where 1 = completely upright, 9 = completely lodged 15

Crop Kind: Faba bean (potential release) Market Class: white flower, small-seeded Variety name: Registration Test ID: 219-16 Yield advantage: 99% of Snowbird (46 sites 6 years) (Snowdrop = 90%) Maturity rating: similar to Snowbird Seed weight: smaller than Snowbird; slightly larger than Snowdrop Quality attributes: (under evaluation) NOT LOW VICINE Agronomic traits: lodging score better than checks; similar height as checks Year of Registration/Release: 2015 registration recommendation 2016 release? NOT ENOUGH SEED 2017 release? DEPENDS ON SEED SUPPLY NOTE: PROGRAM SWITCHING TO LOW VICINE ONLY AS QUICKLY AS POSSIBLE 436 g/1000 seeds 360 g/1000 seeds 335 g/1000 seeds 16

Acknowledgments: Acknowledgments: CSFL Pulse Breeding Staff Kernen Breeder Seed Facility Staff Pulse Genetic Lab Pulse Pathology Research Group Cooperators 17