Report of the ISTA Proficiency Test Committee Günter Müller Year of the Review 2013
Proficiency Test Committee Members Chair - Günter Müller Vice - Didier Demilly Members - Laura Carlson - Sharon Davidson - Sinikka Köylijärvi - Gillian McLaren - Mari Jürmann - Zita Ripka - Håkon Tangerås - Benno Voit - Ruojing Wang - Gisela Wustmann - Rita Zecchinelli
Content Assessment of the regular Proficiency Test Rounds 2013 on Phalaris canariensis, Pisum sativum, Brassica napus and Pinus sylvestris seed - Test leader s introduction - In-round ratings of accredited and voluntary participants - Troubleshooting for poor results - Presentation of the different methods for germination testing used by participants Results of the third Seed Identification Training Test Round Scheduled Proficiency Test Rounds 2014-2015
PT 13-1 Phalaris canariensis Test Leader: Ruojing Wang Scope: P, OSD, G Samples prepared: 3 x 160 Time spent: 236 hours Money spent: 210 $ Date - Presenter Name
PT 13-1 Phalaris canariensis Scope Purity Analysis Accredited Labs N=81 2% 2% Voluntary Labs N=42 5% 2% 7% 96% 86% A B C BMP Date - Presenter Name
PT 13-1 Phalaris canariensis Scope OSD Accredited Labs N=81 Voluntary Labs N=42 5% 2% 26% 33% 31% 62% 17% 24% A B C BMP Date - Presenter Name
PT 13-1 Phalaris canariensis Lot 1 Seeds retrieved and identified Species Seeds added Retrieval Rate (%) Hordeum jubatum 3 86.7 Sporobolus cryptandrus 2 29.3 Eragrostis curvula 2 87.8 Panicum maximum 4 63.8
PT 13-1 Phalaris canariensis Lot 2 Seeds retrieved and identified Species Rapistrum rugosum Seeds added Retrieval Rate (%) 2 95.5 Holcus lanatus 4 73.0 Phalaris arundinacea 4 87.0 Eragrostis tef 3 83.5
PT 13-1 Phalaris canariensis Lot 3 Seeds retrieved and identified Species Alopecurus aequalis Seeds added Retrieval Rate (%) 4 84.3 Galium aparine 3 97.0 Helianthus annuus Lapsana communis 3 93.2 2 91.9
PT 13-1 Phalaris canariensis Scope Germination Testing Accredited Labs Voluntary Labs 1% N=80 N=43 15% 4% 2% 14% 7% 80% 77% A B C BMP Date - Presenter Name
PT 13-1 Phalaris canariensis Germination Test Results Lot 1 2 3 % Normal Seedlings Standard Deviation % Abnormal Seedlings 78.5 89.1 78.7 3.5 2.2 3.2 2.9 2.4 3.3
PT 13-1 Phalaris canariensis Normal Seedlings Distribution 70 60 Frequency 50 40 30 20 10 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-1 Phalaris canariensis Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP 19 +1.1 +0.6 0 +1.7 PP 18-1.1 0-0.7-1.8 TP 55 0-0.2 +0.3 +0.1 O 1-7.4-0.8-0.7-8.9 PT Mean Values 78.4 88.8 78.7
PT 13-1 Phalaris canariensis Applied Germination Test Methods (Temperature) Temp. C Users (accr. Labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 15<=>25 7 +0.2 +1.3 +2.1 +3.6 20 2 +7.6 +1.2 +03 +9.1 20<=>30 84-0.2-0.1-0.2-0.5 PT Mean Values 78.4 88.8 78.7
PT 13-1 Phalaris canariensis Applied Germination Test Methods (Pre-treatment) Pre-treatment Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total KNO3 25-0.1 0.4-0.2 +0.1 Light 2-1.9-2.8-2.7-7.4 Not Treated 29 +0.9 +0.8 +0.9 +2.6 Pre-chill 37-0.6-0.6-0.5-1.7 PT Mean Values 78.4 88.8 78.7
PT 13-2 Pisum sativum Test Leader: Sylvia Zanetti Scope: G Samples prepared 3 x 196 Time spent: 48 hours Money spent:? (no info) Date - Presenter Name
PT 13-2 Pisum sativum Scope Germination Testing Accredited Labs N=89 Voluntary Labs N=66 16% 4% 20% 8% 43% 80% 29% A B C BMP Date - Presenter Name
PT 13-2 Pisum sativum Germination Test Results Lot 1 2 3 % Normal Seedlings Standard Deviation % Abnormal Seedlings 77.5 98.8 93.4 2.7 0.7 1.8 17.1 1.3 5.6
PT 13-2 Pisum sativum Normal Seedlings Distribution Frequency 100 90 80 70 60 50 40 30 20 10 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-2 Pisum sativum Abnormal Seedlings Distribution 140 120 Frequency 100 80 60 40 20 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-2 Pisum sativum Non-germinated Seeds Distribution 160 140 120 Frequency 100 80 60 40 20 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-2 Pisum sativum Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP 43-0.1-0.2-0.1-0.4 PP 6-0.3-0.6-1.4-2.3 TP 1 +2.4-1.6-0.6 +0.2 Sand 53-0.2 +0.3 +0.3 +0.4 Soil 1 +5.4 +0.4 +0.4 +6.2 PT Mean Values 77.6 98.6 93.6
PT 13-1 Pisum sativum Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 20 103-0.1 0 0-0.1 25 1 4.4 0.4 1.4 6.2 PT Mean Values 77.6 98.6 93.6
PT 13-3 Brassica napus Test Leader: Günter Müller Scope: P, OSD, G, TZ, Moi Samples prepared: 6 x 210 Time spent: 259 hours Money spent: 48 Euro Date - Presenter Name
PT 13-3 Brassica napus Scope Purity analysis Accredited Labs N=114 1% 3% Voluntary Labs 9% N=43 14% 77% 96% A B C BMP Date - Presenter Name
PT 13-3 Brassica napus Scope OSD 16% Accredited Labs N=114 2% 4% 23% Voluntary Labs N=43 45% 9% 78% 23% A B C BMP Date - Presenter Name
PT 13-1 Brassica napus Lot 1 Seeds retrieved and identified Species Seeds added Retrieval Rate (%) Galium aparine 4 95.5 Sinapis arvensis 6 63.0 Stellaria media 5 88.8 Thlaspi arvense 5 96.9
PT 13-1 Brassica napus Lot 2 Seeds retrieved and identified Species Chenopodium album Seeds added Retrieval Rate (%) 4 92.8 Lactuca sativa 2 98.7 Phacelia tanacetifolia Vaccaria hispanica 3 93.4 3 45.6
PT 13-1 Brassica napus Lot 3 Seeds retrieved and identified Species Fallopia convolvulus Seeds added Retrieval Rate (%) 2 90.1 Raphanus sativus 3 91.9 Rumex crispus 3 97.7 Sinapis alba 4 91.1
PT 13-3 Brassica napus Scope Germination Testing Accredited Labs 5% 4% N=112 Voluntary Labs 17% N=46 13% 7% 56% 20% 78% A B C BMP Date - Presenter Name
PT 13-3 Brassica napus Germination Test Results Lot 1 2 3 % Normal Seedlings 88.9 90.1 82.8 Standard Deviation % Abnormal Seedlings 2.5 2.5 3.7 9.0 6.5 14.7
PT 13-3 Brassica napus Normal Seedlings Distribution Frequency 100 90 80 70 60 50 40 30 20 10 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-3 Brassica napus Abnormal Seedlings Distribution Frequency 90 80 70 60 50 40 30 20 10 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-3 Brassica napus Non-germinated Seeds Distribution 140 120 Frequency 100 80 60 40 20 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-3 Brassica napus Applied Germination Test Methods (Substrate) Substrate Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total BP 14-2.5-3.1-3.3-8.9 PP 26 +0.5-0.2 +1.6 +1.8 TP 74 +0.4 +0.7 +0.4 +1.6 Sand 1-8.5-6.9-24.2-39.6 O 1-2.5 +5.1-3.2-0.6 PT Mean Values 88.5 89.9 82.2
PT 13-3 Brassica napus Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 20 51-0.5 +0.3-0.9-1.1 20<=>30 64 +0.7-0.1 +1.0 +1.6 25 1-19.5-9.9-18.2-47.6 PT Mean Values 88.5 89.9 82.2
PT 13-3 Brassica napus Applied Germination Test Methods Pre-treatment Pretreatment Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total KNO3 23 +0.3 +0.3 +1.6 +2.3 Light 1 +0.5 +0.1 +2.8 +3.4 Not Treated 61 0 0-0.5-0.5 Pre-chill 31-0.2-0.4-0.3-0.9 PT Mean Values 88.5 89.9 82.2
PT 13-3 Brassica napus Scope Viability Testing Accredited Labs N=49 Voluntary Labs N=56 4% 4% 7% 7% 16% 16% 76% 70% A B C BMP Date - Presenter Name
PT 13-3 Brassica napus Viable Seeds Distribution 70 60 Frequency 50 40 30 20 10 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-3 Brassica napus Scope Moisture Content Determination Accredited Labs N=100 Voluntary Labs N=47 17% 3% 6% 26% 4% 47% 74% 23% A B C BMP Date - Presenter Name
PT 13-3 Brassica napus Moisture Content Distribution 35 30 Frequency 25 20 15 10 5 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT 13-3 Pinus sylvestris Test Leader: Sergio Pasquini Scope: G Samples prepared: 3 x 100 Time spent: 65 hours Money spent: 714 Date - Presenter Name
PT13-3 Pinus sylvestris Scope Germination Testing Accredited Labs N=24 Voluntary Labs N=25 8% 21% 28% 64% 8% 13% 58% A B C BMP Date - Presenter Name
PT13-3 Pinus sylvestris Germination Test Results Lot 1 2 3 % Normal Seedlings Standard Deviation % Abnormal Seedlings 92.3 86.0 90.3 2.5 5.6 2.2 2.2 3.1 2.8
PT13-3 Pinus sylvestris Normal Seedlings Distribution 35 30 Frequency 25 20 15 10 5 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT13-3 Pinus sylvestris Abnormal Seedlings Distribution 35 30 Frequency 25 20 15 10 5 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT13-3 Pinus sylvestris Non-germinated Seeds Distribution 30 25 Frequency 20 15 10 5 0-4,5-4 -3,5-3 -2,5-2 -1,5-1 -0,5 0 0,5 1 1,5 2 2,5 3 3,5 4 4,5 >5 Z-Scores
PT13-3 Pinus sylvestris Applied Germination Test Methods (Substrate) Substrate Not reported Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 1 +2.1 +5.2-2.8 +4.6 PP 3 +0.5-7.5-0.1-7.1 TP 20-0.2 +0.9 +0.2 +0.8 PT Mean Values 92.4 80.1 90.2
PT13-3 Pinus sylvestris Applied Germination Test Methods (Temperature) Temp. C Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 20 3-0.9 +4.2-2.4 +0.9 20<=>30 21 +0.1-0.5 +0.3-0.1 PT Mean Values 92.4 80.1 90.2
PT13-3 Pinus sylvestris Applied Germination Test Methods (Pre-treatment) Pretreatment Not Treated Users (accr. labs) Germinated Seeds (deviation from mean) S1 S2 S3 Total 13-0.5-6.5-0.9-7.9 Pre-chill 11 +0.6 +7.6 +1.1 +9.3 PT Mean Values 92.4 80.1 90.2
Seed Identification Training Test Round Test Leader: Günter Müller Scope: OSD Samples prepared: 150 Time spent: 55 hours Money spent: 25 Date - Presenter Name
Seed Identification Training Test Round 17% Voluntary Labs 3% N=119 3% 77% A B C BMP Date - Presenter Name
Seed Identification Training Test Round (Part 1) Species Seeds added Retrieval Rate (%) Pascopyrum smithii 15 16.8 Crotalaria pallida 7 34.2 Luffa acutangula 11 54.9 Carica papaya 10 59.0 Momordica charantia 11 88.2 Holcus lanatus 10 89.1
Seed Identification Training Test Round (Part 2) Species Trifolium resupinatum Seeds added Retrieval Rate (%) 14 89.3 Sinapis alba 8 93.6 Plantago major 12 93.8 Festuca rubra 10 94.7 Bromus sterilis 11 95.2 Cynoglossum officinale 10 95.3
Seed Identification Training Test Round (Part 3) Species Seeds added Retrieval Rate (%) Bromus sterilis 11 95.2 Geranium dissectum 13 96.4 Aethusa cynapium 8 96.7 Trifolium repens 10 97.3 Lapsana communis 11 97.3 Agrosthemma githago 15 98.1
Seed Identification Training Test Round (Part 4) Species Seeds added Retrieval Rate (%) Rumex acetosella 12 98.3 Convolvulus arvensis Bromus hordeaceus Alopecurus myosuroides 9 98.7 8 98.9 9 99.0 Glycine max 7 99.0
Scheduled Proficiency Test Rounds 2014-2015 Round # Species 14-3 Poa pratensis Scope P, OSD, G Seed Identification Training Test Round 15-1 Glycine max G, M 15-1 Fagus sylvatica P, G, TZ, M, OIC 15-2 Trifolium pratense P, OSD, G, TZ, OIC
Thank you very much for your attention! Date - Presenter Name