Increasing the efficiency of conservation of wild grapevine genetic resources in Europe

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European Cooperative Program for Plant Genetic Resources (ECPGR) Institute for Adriatic Crops and Karst Reclamation, Split Croatia 5th July 2016 Increasing the efficiency of conservation of wild grapevine genetic resources in Europe Current status of Vitis sylvestris genetic resources in Germany Erika Maul and Gloria Ledesma Krist

Introduction Linné did not mention Vitis sylvestris in his Systema Naturae. Wild grape is even today an important genetic resource for quality and after the finding of Kishmish Vatkhana and Dzhandzhal Kara can be considered as a likely resource for resistance breeding. Septentrial populations could be of interest in particular due to their cold tolerance. Photo: Norbert R. Kowarsch

Distribution of the European wild grape Vitis vinifera L. subsp. sylvestris (C. C. Gmelin) Hegi (Portugal to Afghanistan) Distribution of Vitis vinifera L. subsp. sylvestris Habitats published between 1998 and 2010

Bibliographical references about Vitis sylvestris occurence in Germany BOCK (1546): abundantly thriving wild grape between Straßburg and Speyer BRONNER (1857): thousands of individuals occur in the Rhine Valley SCHUMANN (1974): found on the Ketsch-Island 39 wild vines (3 of them were dead) KRAMER (1987): in the Rhine Valley between Basel and Worms only 30 individuals survived. SEBALD et al. (1992) announced in Die Farn- und Blütenpflanzen Baden-Württembergs, that the wild grape was reduced in Baden Württemberg to 25 plants on a few sites between Mannheim and Karlsruhe. ARNOLD et al. (2005) reported about 20 individuals existing in Alsace and the Rhine Valley.

Inventory and reintroduction of Vitis sylvestris Model- und Demonstrationsvorhaben (2008 2013) Undertaking serving as an example for other crops Survival of the wild grape (Vitis vinifera L. subsp. sylvestris) in the Rhine flood plains through a directed in-situ maintenance, financed by the Bundesministerium für Ernährung, Landwirtschaft und Verbraucherschutz (BMELV) via the Bundesanstalt für Landwirtschaft und Ernährung (BLE). Project leader: Gloria Ledesma-Krist from the WWF-Auen-Institut, Karlsruher Institut für Technologie (KIT), Project partners: Botanisches Institut I (KIT), Botanischer Garten Karlsruhe (KIT) and Institut für Rebenzüchtung Geilweilerhof (JKI). Objectives: Safeguard of the remaining Vitis sylvestris diversity in-situ and ex-situ Preservation of the wild grape through reintroduction respectively establishment of viable and diverse populations at selected sites Continuation of evolutionary adaptation processes through reproductive linkage of habitats and the selection of appropriate sites.

Inventory of Vitis sylvestris individuals existing in the German Rhine Valley flood plains. (Stand Dezember 2013) The numbers in brackets represent the planted individuals in the scope of the project. Department Area Wild grape total planted spontaneous Mannheim 59 56 (0) 3 Baden- Württemberg Rhein- land- Pfalz Ketsch 82 3 (1) 79 Regierungsbezirk Karlsruhe ca. 165 ca.164 (0) Otterstadt 7 6 (0) 1 1 Speyer 2 2(0) 0 Rheinland-Pfalz Hessen Römerberg 25 25 (22) 0 Lingenfeld 56 56 (56) 0 Hördt 80 78 (39) 2 Wörth- Maximilliansau Stockstadt am Rhein, Riedstadt 5 5(0) 0 121 121 (121)* 0 Duplication sites of Ketsch population: ex-situ: Geilweilerhof Botanical Garden Karlsruhe (KIT) in-situ: Kühkopf/Hessen 602 516 (239) 86

Historical occurence of Vitis sylvestris in the Rhine Valley Die Wildrebe in Mitteleuropa Diplomarbeit Fritz Schumann 1967 Ketsch Island

The wild grape Vitis sylvestris on the Ketsch Island Photo: Norbert R. Kowarsch Photo: Rudolf Becher

The wild grape Vitis sylvestris on the Ketsch Island

History of synonymy, homonymy and misnaming Ketsch 18 Vitis sylvestris climbing up the oak tree September 2011 Photo: Josef Hermann

History of synonymy, homonymy and misnaming Ketsch 18 Supporting oak tree fell down in 2015 Photo: Norbert R. Kowarsch Photo: Norbert R. Kowarsch

Autumn coloration of leaves Ketsch 96 Photo: Norbert R. Kowarsch Photo: Ursula Brühl, JKI

Collection of seeds Photo: Norbert R. Kowarsch September 2011 October 2015

Ketsch 53 uses 11 supporting trees - collection of seeds

Ketsch 53 uses 11 supporting trees - collection of seeds

History of synonymy, homonymy and misnaming Ketsch 51 Tiny canes climb up the oak tree Young developing shoot Photo: Ursula Brühl, JKI

Rescue of a Ketsch individual October 2012 Photo: Ursula Brühl, JKI Photo: Ursula Brühl, JKI

Rescue of a Ketsch individual October 2012 Photo: Ursula Brühl, JKI Photo: Ursula Brühl, JKI Norbert Krotz, Gloria Ledesma-Krist, Ernst Heene Photo: Ursula Brühl, JKI

Thank you for your attention