KENTUCKY TRIP REPORT Kentucky Plant Exploration Trip. September 15-22, 2016

KENTUCKY TRIP REPORT 2016 Kentucky Plant Exploration Trip September 15-22, 2016

TABLE OF CONTENTS INTRODUCTION 2 DAILY ITINERARY 3 TRIP MEMBERS 3 MAPS 4 TRIP JOURNAL 6 LIST OF TAXA 18 FIELD NOTES 19 Trip report prepared by: Jenna Zukswert Front Image: Oxydendrum arboreum at the Natural Arch Scenic Area, Whitley City, KY (Photo: J. Zukswert) 1

INTRODUCTION The southeastern United States is a notably biodiverse region, providing a variety of habitats for thousands of temperate plant species. For this reason, the American southeast is considered a plantcollecting hotspot for the Arnold Arboretum s Campaign for Living Collections, a 10-year initiative formally launched in 2015 with the goal of collecting nearly 400 taxa from temperate forest regions to add to the Arnold s collections (Friedman et al., 2016). These include taxa that are new to the Arnold, new provenances of taxa that the Arnold collections already contain, taxa that were unsuccessfully propagated at the Arnold in the past, and taxa for which the Arnold does not currently have a wildcollected representative (Friedman et al., 2016). The Daniel Boone National Forest in eastern Kentucky therefore made a satisfactory location to collect for the Campaign. In addition, it is home to northern populations of Stewartia ovata, a shrub native to the southern Appalachians. The genus Stewartia is of particular interest to the Polly Hill Arboretum in West Tisbury, MA as they host the nationally accredited Stewartia collection, which includes nine cultivars named by Polly Hill herself (Polly Hill Arboretum, 2016). Kentucky therefore was also a good place to locate and collect Stewartia ovata in its northern range. In 2016, the Arnold Arboretum partnered with the Polly Hill Arboretum as well at the Mt. Cuba Center in Hockessin, DE, Gainesway Farm in Lexington, KY, and the Arboretum, State Botanical Garden of Kentucky in Lexington on a collaborative, multi-institution expedition to the Daniel Boone National Forest, in search primarily of Stewartia ovata, but also a selection of species targeted by the Arnold Arboretum s Campaign. Expedition team members affiliated with arboretums include Tim Boland (Executive Director, Polly Hill Arboretum), Catherine Meholic (Plant Recorder, Mt. Cuba Center), Jenna Zukswert (Living Collections Fellow, Arnold Arboretum), Phillip Douglas (Director of Horticulture, Gainesway Farm), Todd Rounsaville (Curator, The Arboretum, State Botanical Gardens of Kentucky) and his three colleagues Laura Baird, Robert Castlen, and Michael Patton. Kentucky botanist Julian Campbell and Georgia naturalist Jack Johnston provided valuable local expertise and guided us to many of our collections. In search of Stewartia ovata and the Campaign species, the team explored moist cove forests and the banks of the Cumberland River, among other forest habitats, and were granted permission to collect on private property as well. The trip yielded 44 accessions, 43 from which we obtained seed and 18 of which were Stewartia ovata. These accessions consisted of 21 taxa in total, 11 of which were listed in the desiderata for the Campaign for Living Collections, for which we collected seed from 10. Both objectives of the expedition to locate and collect Stewartia ovata as well as a selection of Campaign species were well met as a result of the kind collaboration of the entire team. Citations Friedman WE, Dosmann MS, Boland TM, Boufford DE, Donoghue MJ, Gapinski A, Hufford L, Meyer PW, Pfister DH. 2016. Developing an Exemplary Collection: A Vision for the Next Century at the Arnold Arboretum of Harvard University. Arnoldia 73: 2-18. Polly Hill Arboretum. 2016. Stewartia Collection. Obtained 5 October 2016 from: <http://www.pollyhillarboretum.org/stewartia-collection/> 2

DAILY ITINERARY Date Location Members Activity 9/15/16 Cumberland Falls State Resort Park, T. Boland, J. Campbell, Travel, Corbin, KY P. Douglas, C. Meholic, Field Collecting 9/16/16 McCreary and Whitley counties, KY (Parkers Lake, Pine Knot, Whitley City) 9/17/16 McCreary and Whitley counties, KY (Cumberland Falls, Yahoo Falls) 9/18/16 Laurel, McCreary, and Whitley counties, KY (Bark Camp Creek, Marsh Branch Rd, The Gulf) 9/19/16 McCreary and Pulaski counties, KY J. Zukswert T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert T. Boland, J. Johnston, C. Meholic, J. Zukswert T. Boland, C. Meholic, (Bauer Rd, Blue Heron Overlook) J. Zukswert 9/20/16 Lexington, KY T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert 9/21/16 Lexington, KY: The Arboretum, State Botanical Garden of Kentucky and Gainesway Farm T. Boland, R. Castlen, P. Douglas, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert Field Collecting Field Collecting Field Collecting Field Collecting Travel, Collection Processing Local Arboretum Visits TRIP MEMBERS Tim Boland Polly Hill Arboretum Julian Campbell Botanist, Kentucky Phillip Douglas Gainesway Farm Jack Johnston Naturalist, Georgia Catherine Meholic Mt. Cuba Center Todd Rounsaville The Arboretum, State Botanical Garden of Kentucky Jenna Zukswert Arnold Arboretum Laura Baird The Arboretum, State Botanical Garden of Kentucky Robert Castlen The Arboretum, State Botanical Garden of Kentucky Michael Patton The Arboretum, State Botanical Garden of Kentucky 3

MAPS Map 1. Ecoregional section map of Kentucky. Map produced by J. Campbell, 2003. 4

Map 2. Locations of the 44 accessions obtained within Kentucky s Daniel Boone National Forest (in green) on this expedition. Stewartia ovata collection locations are in red, and those of all other taxa are in blue. Map produced by J. Zukswert using Google Maps, 2016. 5

TRIP JOURNAL Prepared by Jenna Zukswert Thursday, September 15 Our journey began around 1:00 PM on Thursday, September 15 th in Lexington s Blue Grass Airport. After flying from Boston, Tim and I met up with Catherine (Cat), Phillip (Phil), and Julian near the rental car counter. Upon obtaining our minivan, the vehicle of choice for botanical exploration, soon Tim, Cat, and I were following Phil and Julian on a journey from Lexington to our base camp for the week: Cumberland Falls State Resort Park in Corbin. Spotting several of our target taxa from the highway as we botanized at 60 miles per hour, we felt more confident that our trip would be a success. In a quick change of plans, we decided to start collecting locally that day instead of starting on Friday. Almost immediately after embarking on a narrow trail on the side of Cumberland Falls Road, we stumbled across our primary target species: Stewartia ovata (001). Not far along the trail, we climbed up to a scenic overlook at a stone gazebo, where we found nuts from three different Carya species (002-004) as well as a snake skin, serving as a chilling reminder of the venomous creature that had slithered where we were standing not long before our arrival. We then crossed county lines into McCreary for a short walk along the Cumberland River, where we found more Stewartia ovata (005 and 008), Trauvetteria caroliniensis (006), and Itea virginica (007). Near our last Stewartia collection, we took a moment to look out over the Cumberland River and admire our beautiful surroundings. Eight accessions, with two opportunities for scenic overlooks not bad for the very first day! Snake skin at our first collection site (Photo: T. Boland) Thursday night marked our first of many buffets at the Cumberland Falls State Resort Park restaurant and a gathering in the lodge to process the herbarium specimens, with plenty of pawpaw fruits and plums from Julian to sustain us. Later that night, Tim, Cat and I drove down to the Cumberland Falls proper and witnessed a moonbow, a subtle arc of moonlight hovering over rocks at the bottom of the waterfall that is only visible during full moons a perfect way to end a successful first day. Collections Made 001 Stewartia ovata 005 Stewartia ovata 002 Carya tomentosa 006 Trautvetteria caroliniensis 003 Carya glabra 007 Itea virginica 004 Carya ovata 008 Stewartia ovata 6

Left: View of the Cumberland River from our second collection site of the day (Photo: J. Zukswert) Right: Top Cat Meholic collects Trautvetteria caroliniensis seed (006). Bottom Julian empathizes with a tree possessing an enormous burl (Photos: T. Boland) Left: Herbarium voucher of Stewartia ovata (005, Photo: J. Zukswert) Top: Phil and Tim tighten the straps of the herbarium press after assembling the vouchers (Photo: J. Zukswert) 7

Friday, September 16 After a breakfast buffet at the resort, Tim, Phil, Julian, Cat, and I piled in the minivan and drove through the town of Sawyer to reach Good Spring Farm. Good Spring Farm is a bed and breakfast owned by Peg Taylor, wife of the late Norman L. Taylor from the University of Kentucky, who had been one of Julian s thesis committee members. Peg welcomed us into her lovely home and granted us permission to collect fruit from her Stewartia ovata trees in the forest on her property (009 and 010). Shortly after, we walked along a path off route 700 along Indian Creek, where we found a small population of Stewartia ovata (011). After driving along winding roads, passing the Blazin Bluegrass festival, and stopping for lunch at Subway, we visited a private property in Pine Knot. This property, belonging to Pam Meadors, was one of the few areas where Julian had seen Malus angustifolia in the wild. After asking permission of her nephew, we collected fruits from the tree (012) and walked a little further down the road to collect from another (013). Upon returning from our short venture, we discovered that Pam was now home, did indeed remember Julian, and gave us permission to collect Quercus velutina (014), Quercus falcata (015), and Quercus montana (016) from her yard as well. Given the size of the trees and the age of the house, it was reasonable to assume that these oaks in her yard were wild. She assisted in acorn gathering as well, meriting an official designation as a collector. Tempted to get another Q. falcata specimen, since the tree from which we collected acorns was too tall to obtain an herbarium voucher, we found another (017) at Laurel Cemetery, where we also admired moss blanketing the cemetery, an abundance of yucca, and a large Quercus marilandica next to our Q. falcata collection. Following that collection, we spotted Magnolia macrophylla while botanizing from the van, prompting us to turn around on route 478 and collect from these roadside trees (018). After an ultimately unsuccessful but pleasant hike through a roadside hemlock-rhododendron habitat, we chose Natural Arch Scenic Area as our final destination for the day. Delighted to see Carya pallida in the parking lot at the overlook, we were disappointed to find that weevils had discovered all of the nuts before we did, and so we could not collect viable seed. Despite the lack of fruit, we collected an herbarium voucher and recorded the location of this species for future reference. Our day ended successfully, though, as we collected seed from Oxydendrum arboreum with gorgeous, deep red foliage within sight of the Natural Arch (see front cover). Later that night, after our customary buffet dinner, we met up with Jack Johnston, who arrived with a trunk-load of Magnolia macrophylla fruit from Alabama. While repacking our herbarium specimens and typing up field notes, we enjoyed another night of winding down at the lodge after a busy day of exploring. Collections Made 009 Stewartia ovata 015 Quercus falcata 010 Stewartia ovata 016 Quercus montana 011 Stewartia ovata 017 Quercus falcata 012 Malus angustifolia 018 Magnolia macrophylla 013 Malus angustifolia 019 Carya pallida 014 Quercus velutina 020 Oxydendrum arboreum 8

Left: Kentucky-themed embroidery at Good Spring Farm in McCreary county (Photo: T. Boland) Right: Top Phil, Cat, and Jenna process a Stewartia ovata collection (009) at Good Spring Farm. Bottom close-up of Stewartia ovata fruit collected at Good Spring Farm (Photos: T. Boland) Left: Quercus marilandica, a large tree observed adjacent to Quercus falcata (017) at Laurel Cemetery (Photo: T. Boland) Top: Julian and Phil look on as Tim regrets his decision to taste Malus angustifolia (012, Photo: J. Zukswert) 9

Saturday, September 17 Our group grew on Saturday as we met up with Todd Rounsaville of The Arboretum, State Botanical Garden of Kentucky through the University of Kentucky, and three of his colleagues: Laura Baird, Michael Patton, and Robert Castlen. Our group of ten spent the morning exploring the acidophilic, Rhododendron maximum- and Kalmia latifolia-filled banks of the Cumberland River, collecting nine accessions, including one Stewartia ovata (025). We admired many of the herbaceous plants along this trail, including Galax urceolata (024) and Goodyera pubescens (028). At one point on the trek, Julian, who had ventured ahead of us, ran back to our group to announce that he had found a copperhead snake! Half of the group split to join him and see the snake, while the other half, myself included, took that as our cue to exit the trail and return to the parking lot. Our cautious group did encounter a copperhead slithering past us as we exited the trail, but that was much fewer than the four that Julian s group of courageous explorers found upon returning to the location of the first copperhead. Yikes! After a picnic lunch at Cumberland Falls, we drove to the moist cove forest of Yahoo Falls and took the loop trail. We saw Pachysandra procumbens, though it did not have seed, as well as Cladrastis kentukea, the species featured on the cover of our field notebook. This too did not have seed. The first Cladrastis we saw reached down to us from the top of a rock outcrop, literally living on the edge. It is amazing just how resilient many of these plants can be, the ones that live on the side of rocky slopes in these cove forests. We were lucky that we had a steep metal staircase to help us confront the steep slopes and rock faces that we encountered, enabling us to admire the colossal Fagus grandifolia trees and abundance of Rhododendron maximum and Kalmia latifolia of the cove forest as we descended. Even though this dry summer had caused the falls to become barely a trickle, Yahoo Falls was impressive. We spent some time exploring the rock house behind the falls, admiring the colors and formations of the rocks. Further on, we did encounter another Stewartia ovata (030) and collected from it. When we encountered a light rain as the time approached 4:30 PM, we decided to stop for the day. The group disbanded after another productive day, with Phil, Julian, and the University of Kentucky group returning to Lexington and Tim, Jack, Cat, and I returning to the resort. Upon returning and indulging in the usual buffet, Tim and Cat replaced the newspaper in the herbarium specimens with fresh, dry ones (while keeping a close eye on the Michigan State vs. Notre Dame football game) while Jack and I mapped out locations to collect Stewartia ovata the next day. The evening concluded with an engaging discussion of the cultivar trade as well as Jack s incredible, heartrending stories from his job as an ER nurse in Northern Georgia. Collections Made 021 Liquidambar styraciflua 026 Rhododendron maximum 022 Itea virginica 027 Rhododendron maximum 023 Rhododendron maximum 028 Goodyera pubescens 024 Galax urceolata 029 Kalmia latifolia 025 Stewartia ovata 030 Stewartia ovata 10

Field Notes Left: The team descends the steep metal staircase at Yahoo Falls (Photo: J. Zukswert) Right: Top Phil, Cat, and Jenna make a collection of Liquidambar styraciflua at Cumberland Falls. Bottom The group explores Yahoo Falls (Photos: T. Boland) Left: Todd Rounsaville observes a grape vine near the Cumberland River (Photo: T. Boland) Top: Cladrastis kentukea on the edge (Photo: J. Zukswert) 11

Sunday, September 18 After a leisurely breakfast at the resort, the small group consisting of Tim, Cat, Jack, and I set out for a daylong search for Stewartia ovata populations, which Jack and I had mapped out the night before. The first stop of the day was along Bark Camp Creek. Right upon exiting the minivan and entering the trail off the side of the Forest Service road, Jack immediately spotted our target species. This small tree did not have fruit, so we ventured on in the rain, careful not to slip in the clay and unnerved by the abundance of possible snake holes we saw that reminded us of the copperheads we had observed by the river the day before. Jack spotted another tree, this time with more fruit, and so we collected from it (031), admiring and making note of the small Stewartia ovata seedlings surrounding it that suggest the beginnings of a promising future population. Just as we got in the minivan and prepared to head onwards, Jack spotted another Stewartia ovata, this time right by the road. We collected it (032) then walked further along the road, down the valley closer to Bark Camp Creek, and found two more accessions of Stewartia ovata beyond that (033 and 034), as well as the largest Stewartia ovata Jack had seen in this part of Kentucky. We were pleased to have made four Stewartia ovata collections at our very first stop of the day! Our next stop was Holly Bay Campground, where Jack knew of a Stewartia ovata location up the ravine near the boat ramp. Confronted by a gate, we spoke with a kind woman who gave us a 15-minute visitor pass, and spoke among ourselves of her Southern hospitality as we drove through the campground. We discovered that this hospitality was quite ephemeral as we sat in the parking lot eating our peanut butter sandwiches when we were approached by the same woman, this time driving up to us in a white truck to warn us that our 15 minutes were up and that we had to leave immediately. While we did not make a collection here, it was a nice place to enjoy a quick lunch. Further north, we stopped along the side of Marsh Branch road, north of the boat ramp, and entered the oak-dominated forest. I tried my best to avoid the Smilax and tried not to think of the snakes that may be lurking in the decomposing logs beneath my feet as I made sure to keep up with Jack s pace, realizing I was a bit disoriented and may become lost if I fell behind. As Jack promised, we did find two more Stewartia ovata populations here (035 and 036). After being incredibly misdirected by the minivan s built-in GPS system, which took us to a deadend road in seemingly the middle of nowhere instead of the Bee Creek Market we sought, we refueled our minivan and drove on to The Gulf. Almost immediately after starting along the trail, we discovered a large population of Stewartia ovata in a relatively open area perhaps our best collecting location of Stewartia ovata yet (037). At one more stop on the way back to Cumberland Falls, in a roadside forest full of poison ivy, we collected our last of eight Stewartia ovata accessions that day (038), and then returned yet again to the lodge to process the herbarium specimens and type up the field notes. Collections Made: 031 Stewartia ovata 035 Stewartia ovata 032 Stewartia ovata 036 Stewartia ovata 033 Stewartia ovata 037 Stewartia ovata 034 Stewartia ovata 038 Stewartia ovata 12

Field Notes Left: Tim and Jack collect fruit from Stewartia ovata at Marsh Branch Road (036, Photo: J. Zukswert) Right: The high-tech, button-filled interior of the minivan, complete with a low-tech, banana-based method for propping up the iphone GPS (Photo: J. Zukswert) Left: Jack and Cat search for a good way to enter the forest along Louie Tucker Road in search of Stewartia ovata (038, Photo: J. Zukswert) Right: Trail at The Gulf, where we found our largest Stewartia ovata population (037, Photo: J. Zukswert) 13

Monday, September 19 Following breakfast with Jack, the team for the day, which consisted of Tim, Cat, and myself, embarked on a search for the elusive Cladrastis kentukea. We first tried to navigate to a location that Julian had provided us. We did find Cladrastis kentukea, though again with no seed, and passed some interesting houses in the neighborhood with prominent, decorative banana plants outside as we searched. We gave up our search at this location after tromping through remarkably sticky clay that required the use of sticks to remove it from our boots and decided to turn to the map of Cladrastis kentukea herbarium vouchers that Arnold Arboretum fellow Robert Dowell had prepared for us. Our first voucher site at Sloane Valley was also fruitless, we discovered as we drove through a small neighborhood and clearly agitated the dogs keeping watch in doing so, and so decided to try another near Jasper Bend, closer to the Cumberland River. On the road to Jasper Bend, we stopped to collect Pinus virginiana (039) as well as Diospyros virginiana (040), which we identified while botanizing at 20 miles per hour from the minivan. In the neighborhood at Jasper Bend, I did spot from the minivan a small Cladrasitis kentukea tree in the location Robert had indicated, but there was no seed, and we decided not to investigate further so as to not unnecessarily alarm the neighborhood residents who were out and about. Although we were satisfied with the 40 accessions we had obtained, we decided to choose one more last location to explore after lunch, just to see what we could find. This spot ended up being the Blue Heron trails in Stearns, Kentucky. A large black snake, perhaps a cottonmouth, snoozing on the middle of the trail deterred us from venturing far along the Blue Heron Tipple Trail, so we decided to drive a bit further to the Blue Heron Overlook for one last walk and exploration. Along this walk, I unexpectedly stumbled across a Stewartia ovata tree (041), hidden under a heap of grapevines, next to an Ilex opaca (which had been present near almost every Stewartia ovata we had found on the trip). Tim then found several more populations (including 042), and Cat found a nice Oxydendrum arboreum further along (043). After collecting these, and a hardy Vaccinium arboreum found in a relatively exposed area, we enjoyed the views provided by the Blue Heron Overlook, satisfied with our findings. The day ended with a drive past an interesting plant community along a railroad and further field note and herbarium processing in the lodge that night. Collections Made: 039 Pinus virginiana 042 Stewartia ovata 040 Diospyros virginiana 043 Oxydendrum arboreum 041 Stewartia ovata 044 Vaccinium arboreum 14

Field Notes Left: Diospyros virginiana (040) found on the way to Jasper Bend (Photo: J. Zukswert) Right: Top The view from Blue Heron Overlook. Bottom Vaccinium arboreum (044, Photos: T. Boland) Left: Jenna and Tim pose by the unexpected Stewartia ovata (041) discovery (Photo: C. Meholic) Right: Cat prepares to collect Oxydendrum arboreum (043) at Blue Heron Overlook (Photo: J. Zukswert) 15

Tuesday, September 20 Field collecting was over, and it was time to reunite in Lexington to end the expedition. On Tuesday morning Tim, Cat and I checked out of Cumberland Falls resort, with some difficulty relating to computer problems, and drove to our Airbnb location in Lexington, which was within walking distance of the Northside neighborhood and not far from downtown Lexington. We indulged in dessert before lunch by visiting North Lime Coffee & Donuts before eating at Minton s, a local restaurant. We then met up with Phil and Julian back at our new Airbnb headquarters and divided the seed between our institutions, using the coffee table in the living room as our work surface. After everything was shipped back north through FedEx, and the minivan seemed emptier than ever, Tim, Cat and I joined Phil and his wife for a dinner at Willie s BBQ, and were able to spend a night not processing herbarium specimens or typing up field notes. Wednesday, September 21 On our last full day in Kentucky, we had the privilege of touring both The Arboretum, State Botanical Garden of Kentucky and Gainesway Farm. Todd, Michael, and Robert greeted Tim, Cat, Phil, and me at the visitor center for the Arboretum and took us on a walking tour through the diverse plant ecosystems of Kentucky, which are all represented on the grounds at the Arboretum. We admired oaks dating back to the 1700s as well as the new plantings that will, over time, become impressively large trees themselves. Gainesway Farm was our next stop, where Phil led us around the impressive landscape. We explored the award-winning barns, gawked at horses worth millions, and admired the beautiful plantings and collections on the grounds, including the incredible oak collection The day, and expedition itself, ended with dinner at Mi Pequeña Hacienda and then the parting of ways that night and the next morning. The Kentucky group remained in their state, while Tim headed to Michigan, I headed to Tennessee for a subsequent plant-collecting trip, and Cat headed back to Delaware. Conclusion On this expedition, we collected 44 accessions, which includes 21 different taxa. Meeting our primary objective of locating and collecting Stewartia ovata, we managed to collect 18 separate accessions of this target species. In addition, we were able to find viable seed from 10 of the taxa listed in the Arnold Arboretum s desiderata for the Campaign for Living Collections, and obtained herbarium vouchers for 11. This trip was a success due most importantly to the kind collaboration and diverse expertise of our multi-arboretum team. Plant-collecting expeditions have a tendency to showcase a generous and remarkably collaborative side to conservation and curation, and this expedition was no exception. Many thanks to everyone who participated and made this trip possible. 16

Left: Bridge at the Arboretum, State Botanical Garden of Kentucky (Photo: J. Zukswert) Right: Top The oak collection at Gainesway Farm. Bottom: Cat and Jenna prepare to meet their new equine friends at Gainesway Farm (Photos: T. Boland) Left: Cat, Tim, and Phil divide a collection of Stewartia ovata capsules (Photo: J. Zukswert) Right: The minivan, loaded with boxes of seed, herbarium specimens, and supplies to ship back to the northeast (Photo: J. Zukswert) 17

LIST OF TAXA ARNOLD ARBORETUM Species Collection Number(s) Date(s) Collected New Species New Provenance Finally Wild Carya glabra TUCK16-003 15-SEPT Carya ovata TUCK16-004 15-SEPT NA NA NA Carya pallida TUCK16-019 16-SEPT NA NA NA Carya tomentosa TUCK16-002 15-SEPT Diospyros virginiana TUCK16-040 19-SEPT NA NA NA Galax urceolata TUCK16-024 17-SEPT NA NA NA Goodyera pubescens TUCK16-028 17-SEPT NA NA NA Itea virginica TUCK16-007, 022 15-SEPT, 17-SEPT Kalmia latifolia TUCK16-029 17-SEPT Liquidambar styraciflua TUCK16-021 17-SEPT Magnolia macrophylla TUCK16-018 16-SEPT NA NA NA Malus angustifolia TUCK16-012, 013 16-SEPT Oxydendrum arboreum TUCK16-020, 043 16-SEPT, 19-SEPT Pinus virginiana TUCK16-039 19-SEPT Quercus falcata TUCK16-015, 017 16-SEPT Quercus montana TUCK16-016 16-SEPT NA NA NA Quercus velutina TUCK16-014 16-SEPT Rhododendron maximum TUCK16-023, 026, 17-SEPT 027 Stewartia ovata TUCK16-001, 005, 008, 009, 010, 011, 025, 030, 031, 032, 033, 034, 035, 036, 037, 038, 041, 042 15-SEPT, 16-SEPT, 17-SEPT, 18-SEPT, 19-SEPT Trautvetteria caroliniensis TUCK16-006 15-SEPT NA NA NA Vaccinium arboreum TUCK16-044 19-SEPT NA NA NA Red = seed missing or not enough seed after collecting trip 18

FIELD NOTES Soils data from USDA Natural Resource Conservation Service; photos by T. Boland and J. Zukswert Field Collection Number: TUCK16-001 Scientific Name: Stewartia ovata Date: 15 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 1262 ft Latitude: 36⁰ 50.623 N Longitude: 84⁰ 19.812 W Habitat Notes: From side of path through forest near Cumberland Falls Rd, two trees were collected from on either side of the road Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Sequoia complex, 20-35 % slopes, rocky (sandstone/shale bedrock) Plant Description: One accession for four trees, trees cut back from the path Height: 12-15 ft DBH: 2 in Bark: Gray, striped Leaves: Green, ovate, hairs on underside and petiole Fruit: Brown and green capsules, hairs on capsules Seed Collected from: 4 plants Herbarium Specimens: 3 Associated Species: Stewartia ovata, Ilex opaca, Kalmia latifolia, Cornus florida, Fagus grandifolia, Sassafras albidum, Magnolia macrophylla, Liriodendron tulipifera Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA (Arnold Arboretum) Accession Number: 798-2016 PHA (Polly Hill Arboretum) Accession Number: 2016-79 19

Field Collection Number: TUCK16-002 Scientific Name: Carya tomentosa Date: 15 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 1316 ft Latitude: 36⁰ 50.647 N Longitude: 84⁰ 19.787 W Habitat Notes: Found at the top of the trail in the forest near Cumberland Falls Rd, nuts collected from the ground Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Sequoia complex, 20-35 % slopes, rocky (sandstone/shale bedrock) Plant Description: One tree Height: Not recorded DBH: 10 in Bark: Gray, more deeply furrowed than C. glabra Leaves: Not recorded Fruit: Segmented husk Biomass Type: Seed Seed Collected From: ground near 1 plant Herbarium Specimens: 3 Associated Species: Nyssa sylvatica, Oxydendrum arboreum, Liquidambar styraciflua, Quercus velutina, Quercus rubra, Carya pallida, Carya glabra, Carya ovata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 799-2016 PHA Accession Number: N/A 20

Field Collection Number: TUCK16-003 Scientific Name: Carya glabra Date: 15 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 1316 ft Latitude: 36⁰ 50.647 Longitude: 84⁰ 19.787 Habitat Notes: Found at the top of the trail in the forest near Cumberland Falls Rd, nuts collected from the ground Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Sequoia complex, 20-35 % slopes, rocky (sandstone/shale bedrock) Plant Description: Tree Height: Not recorded DBH: 8 in Bark: Gray Leaves: Green, compound pinnate, no fall color Fruit: Dark brown Seed Collected from: Ground near 1 tree Herbarium Specimens: None Photograph: No Associated Species: Nyssa sylvatica, Oxydendrum arboreum, Liquidambar styraciflua, Quercus velutina, Quercus rubra, Carya pallida, Carya glabra, Carya ovata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 800-2016 PHA Accession Number: N/A 21

Field Collection Number: TUCK16-004 Scientific Name: Carya ovata Date: 15 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 1316 ft Latitude: 36⁰ 50.647 N Longitude: 84⁰ 19.787 W Habitat Notes: Found at the top of the trail in the forest near Cumberland Falls Rd, nuts collected from the ground Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Sequoia complex, 20-35 % slopes, rocky (sandstone/shale bedrock) Plant Description: Didn t see tree Height: Not recorded DBH: Not recorded Bark: Not recorded Leaves: Not recorded Fruit: Not recorded Seed Collected from: Ground Herbarium Specimens: None Photograph: No Associated Species: Nyssa sylvatica, Oxydendrum arboreum, Liquidambar styriciflua, Quercus velutina, Quercus rubra, Carya pallida, Carya glabra, Carya ovata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 22

Field Collection Number: TUCK16-005 Scientific Name: Stewartia ovata Date: 15 September 2016 County: McCreary Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 899 ft Latitude: 36⁰ 50.245 N Longitude: 84⁰ 20.723 W Habitat Notes: On the side of Eagle Falls trail, by Cumberland River Slope: Not recorded Aspect: Not recorded Soil Type: Rigley-Alticrest complex, 20-80% slopes, very rocky (sandstone/shale bedrock) Plant Description: One multi-stemmed tree Height: 20 ft DBH: 2 in Bark: Gray, striped Leaves: Green, some leaves have purple spots, hairs on leaf underside and petiole, lustrous and waxy adaxial leaf surface Fruit: Brown and green capsules, hairs Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Alnus serrulata, Kalmia latifolia, Apios americana, Carpinus caroliniana, Rhododendron arborescens, Acer rubrum, Fagus grandifolia, Asimina triloba Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 801-2016 PHA Accession Number: 2016-80 23

Field Collection Number: TUCK16-006 Scientific Name: Trautvetteria caroliniensis Date: 15 September 2016 County: McCreary Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 899 ft Latitude: 36⁰ 50.245 N Longitude: 84⁰ 20.723 W Habitat Notes: On the side of Eagle Falls trail, by Cumberland River Slope: Not recorded Aspect: Not recorded Soil Type: Rigley-Alticrest complex, 20-80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Multi-stemmed, herbaceous Height: 5 ft DBH: 0.25 in Bark: No bark Leaves: Light green Fruit: Not recorded Seed Collected from: 1 plant Herbarium Specimens: None Photograph: No Associated Species: Alnus serrulata, Kalmia latifolia, Apios americana, Carpinus caroliniana, Rhododendron arborescens, Acer rubrum, Fagus grandifolia, Asimina triloba Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 24

Field Collection Number: TUCK16-007 Scientific Name: Itea virginica Date: 15 September 2016 County: McCreary Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 850 ft Latitude: 36⁰ 50.255 N Longitude: 84⁰ 20.726 W Habitat Notes: On the side of Eagle Falls trail, by Cumberland River Slope: Not recorded Aspect: Not recorded Soil Type: Rigley-Alticrest complex, 20-80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Shrub Height: 4 ft DBH: 0.25 in Bark: Grayish brown, new stems are green Leaves: Green, bristles on leaf edge, glabrous underside Fruit: Green and brown racemes Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Ilex opaca, Rhododendron maximum Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 802-2016 PHA Accession Number: 2016-110 25

Field Collection Number: TUCK16-008 Scientific Name: Stewartia ovata Date: 15 September 2016 County: McCreary Local Name: Daniel Boone National Forests: Cumberland Falls State Park Altitude: 1031 ft Latitude: 36⁰ 50.362 N Longitude: 84⁰ 20.761 W Habitat Notes: Eagle Falls trail, mid/upper-slope, in a hemlock forest Slope: Not recorded Aspect: Not recorded Soil Type: Rigley-Alticrest complex, 20-80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Single tree Height: 20 ft DBH: 3 in Bark: Gray, striped Leaves: Green, ovate, hairs along edge, waxy adaxial leaves Fruit: Brown capsules, green capsules, hairs on edge Biomass Type: Seed Seed Collected From: 1 plant Herbarium Specimens: 3 Associated Species: Tsuga canadensis, Asimina triloba, Carya palllida?, Liquidambar styraciflua, Quercus velutina, Quercus alba Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 803-2016 PHA Accession Number: 2016-81 26

Field Collection Number: TUCK16-009 Scientific Name: Stewartia ovata Date: 16 September 2016 County: McCreary Local Name: Good Spring Farm Altitude: 1053 ft Latitude: 36⁰ 53.116 N Longitude: 84⁰ 19.715 W Habitat Notes: Side of trail behind Good Spring Farm (bed and breakfast owned by Peg Taylor). Several Stewartia ovata seedlings (about 2 ft tall) were observed beneath Slope: About 10 ⁰ (slight, hardly any slope) Aspect: NNE Soil Type: Rigley-Alticrest complex, 20 to 80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Small trees, possibly clonal collected 2 full bags of seed Height: 15 ft DBH: 0.75 in Bark: Gray, striped Leaves: Green, ovate, lustrous adaxial side, hairy underside Fruit: Green and brown capsules, hairs Biomass Type: Seed Seed Collected From: About a dozen plants Herbarium Specimens: 3 Associated Species: Kalmia latifolia, Gaultheria procumbens, Quercus montana, Acer rubrum, Gaylussacia baccata, Pinus virginiana, Amelanchier arborea, Quercus alba Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 804-2016 PHA Accession Number: 2016-82 27

Field Collection Number: TUCK16-010 Scientific Name: Stewartia ovata Date: 16 September 2016 County: McCreary Local Name: Good Spring Farm Altitude: 1122 ft Latitude: 36⁰ 53.110 N Longitude: 84⁰ 19.701 W Habitat Notes: Side of trail behind Good Spring Farm (bed and breakfast owned by Peg Taylor). Several Stewartia ovata seedlings (about 2 ft tall) were observed beneath Slope: About 10⁰ (very slight) Aspect: NNE Soil Type: Rigley-Alticrest complex, 20 to 80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Multiple small trees, had been cut back and sprouted, surrounded by many small Stewartia seedlings (1 full bag) Height: 15 ft DBH: 0.75-1 in Bark: Gray, striped Leaves: Green, lustrous adaxial side Fruit: Brown and green capsules, hairy Seed Collected from: 5 plants Herbarium Specimens: 3 Associated Species: Quercus alba, Tsuga canadensis, Acer rubrum, Ilex opaca, Fagus grandifolia, Carya tomentosa, Quercus montana, Carya glabra Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 805-2016 PHA Accession Number: 2016-83 28

Field Collection Number: TUCK16-011 Scientific Name: Stewartia ovata Date: 16 September 2016 County: McCreary Local Name: Indian Creek Altitude: 964 ft Latitude: 36⁰ 48.094 N Longitude: 84⁰ 22.450 W Habitat Notes: On side of trail near Indian Creek, Rt. 700 Slope: 0-10⁰, hardly any slope Aspect: SW to river (slight slope) Soil Type: Rigley-Alticrest complex, 20 to 80% slopes, very rocky (sandstone/shale bedrock) Plant Description: Multiple trees sporadic throughout area - collected seed from multiple trees, collected herbarium specimens from one Height: 10-15 ft DBH: 1 in Bark: Gray, striped Leaves: Green, lustrous adaxial leaf surface, hairy underside Fruit: Brown and green capsules, hairy Seed Collected from: 4 plants Herbarium Specimens: 3 Associated Species: Hamamelis virginiana, Magnolia macrophylla, Acer rubrum, Quercus alba, Ilex opaca, Fagus grandifolia, Sanguinaria canadensis, Nyssa sylvatica, Tsuga canadensis Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 806-2016 PHA Accession Number: 2016-84 29

Field Collection Number: TUCK16-012 Scientific Name: Malus angustifolia Date: 16 September 2016 County: McCreary Local Name: Pam Meador s property Altitude: 1459 ft Latitude: 36⁰ 40.571 N Longitude: 84⁰ 24.991 W Habitat Notes: Side of Rt 92, on Pam Meadors's property (age of tree vs. date the home was built allowed us to comfortably say this is indeed a wild specimen) Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Single tree Height: 15 ft DBH: 5 in Bark: Gray, striped Leaves: Green, some yellowing and browning of foliage, glabrous Fruit: Green, bitter taste Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Pinus echinata, Liriodendron tulipifera, Cercis canadensis, Ligustrum sinense, Smilax rotundifolia, Sassafras albidum Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 807-2016 PHA Accession Number: N/A 30

Field Collection Number: TUCK16-013 Scientific Name: Malus angustifolia Date: 16 September 2016 County: McCreary Local Name: Roadside, along 2792 Altitude: 1429 ft Latitude: 36⁰ 40.586 N Longitude: 84⁰ 24.748 W Habitat Notes: Roadside, east of TUCK16-012, disturbed roadside site with lots of invasives Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Two trees, looks like it has scab Height: 12 ft DBH: 3 in Bark: Gray, lenticels Leaves: Green, spots, largely defoliated Fruit: Green, small, some spots Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Pinus echinata, Acer rubrum, Carya glabra, Diospyros virginiana, Fraxinus americana Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 808-2016 PHA Accession Number: N/A 31

Field Collection Number: TUCK16-014 Scientific Name: Quercus velutina Date: 16 September 2016 County: McCreary Local Name: Pam Meador s property Altitude: 1447 ft Latitude: 36⁰ 40.574 N Longitude: 84⁰ 25.019 W Habitat Notes: Front yard of Pam Meadors's property (age of tree vs. date the home was built allowed us to comfortably say this is indeed a wild specimen) Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Single tree Height: 70 ft DBH: 30 in Bark: Dark gray, deep furrows Leaves: Yellow-ish petiole, glabrous green leaf, pubescence on petiole Fruit: Not recorded Seed Collected From: 1 plant Herbarium Specimens: 3 Associated Species: Carya tomentosa, Malus angustifolia, Cornus florida, Liquidambar styraciflua Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert, P. Meadors AA Accession Number: 809-2016 PHA Accession Number: N/A 32

Field Collection Number: TUCK16-015 Scientific Name: Quercus falcata Date: 16 September 2016 County: McCreary Local Name: Pam Meador s property Altitude: 1427 ft Latitude: 36⁰ 40.577 N Longitude: 84⁰ 25.033 W Habitat Notes: Backyard of Pam Meador s property Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Single tree Height: 80 ft DBH: 35 in Bark: Gray, furrowed, lichen Leaves: Glabrous, green, turkey foot Fruit: Small, brown acorns Seed Collected from: 1 plant Herbarium Specimens: None Associated Species: Quercus alba, Quercus coccinea, Quercus montana, Quercus stellata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert, P. Meadors AA Accession Number: 810-2016 PHA Accession Number: 2016-85 33

Field Collection Number: TUCK16-016 Scientific Name: Quercus montana Date: 16 September 2016 County: McCreary Local Name: Pam Meador s property Altitude: 1427 ft Latitude: 36⁰ 40.577 N Longitude: 84⁰ 25.033 W Habitat Notes: Backyard of Pam Meador s property Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Single tree, dead top Height: 60 ft DBH: 25 in Bark: Gray, furrowed Leaves: Green, scalloped edges, orange petioles Fruit: Green, larger acorn Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Quercus alba, Quercus coccinea, Quercus montana, Quercus stellata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert, P. Meadors AA Accession Number: N/A PHA Accession Number: 2016-86 34

Field Collection Number: TUCK16-017 Scientific Name: Quercus falcata Date: 16 September 2016 County: McCreary Local Name: Laurel Cemetery, 10556 Williamsburg Rd Altitude: 1294 ft Latitude: 36⁰ 43.175 N Longitude: 84⁰ 22.359 W Habitat Notes: Center of cemetery Slope: About 15⁰ Aspect: SE Soil Type: Shelocta-Sequoia complex, 20 to 35% slopes, rocky Plant Description: One tree, trunk split Height: 50 ft DBH: 52 in Spread: 75 ft Bark: Gray, furrowed, lichen Leaves: Green, glabrous, turkey foot Fruit: Small brown acorns Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Quercus marilandica, Quercus velutina, Pinus echinata, Quercus stellata, Nyssa sylvatica Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: 811-2016 PHA Accession Number: 2016-87 35

Field Collection Number: TUCK16-018 Scientific Name: Magnolia macrophylla Date: 16 September 2016 County: McCreary Local Name: Rt. 478 Altitude: 1095 ft Latitude: 36⁰ 43.700 N Longitude: 84⁰ 24.374 W Habitat Notes: On side of Rt. 478 Slope: About 45⁰ Aspect: S Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Four trees Height: 20-25 ft DBH: 2-4 in Bark: Smooth, tan Leaves: Large, green, glabrous, some brown and yellow spots Fruit: Large capsule with red seed Biomass Type: Seed Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Liriodendron tulipifera, Quercus alba, Carya glabra, Cornus florida, Diospyros virginiana Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 36

Field Collection Number: TUCK16-019 Scientific Name: Carya pallida Date: 16 September 2016 County: McCreary Local Name: Natural Arch Scenic Overlook Altitude: 1329 ft Latitude: 36⁰ 50.453 N Longitude: 84⁰ 30.701 W Habitat Notes: Parking lot of Natural Arch area Slope: About 15⁰ Aspect: NNE Soil Type: Wernock-Sequoia complex, 12 to 25% slopes (siltstone bedrock) Plant Description: Single tree, but there are several in this area Height: 50 ft DBH: 9 in Bark: Gray, furrowed Leaves: Compound, pinnate, silvery scales and orange glands on underside Fruit: Brown not viable, so not collected Biomass Type: None Seed Collected From: None Herbarium Specimens: 3 Associated Species: Carya pallida, Cornus florida, Pinus virginiana, Diospyros virginiana, Quercus alba, Nyssa sylvatica, Pinus echinata Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 37

Field Collection Number: TUCK16-020 Scientific Name: Oxydendrum arboreum Date: 16 September 2016 County: McCreary Local Name: Natural Arch Scenic Overlook Altitude: 1279 ft Latitude: 36⁰ 50.557 N Longitude: 84⁰ 30.749 W Habitat Notes: Natural Arch overlook area, on edge facing arch Slope: Maybe 50⁰? Very steep Aspect: N Soil Type: Wernock-Sequoia complex, 12 to 25% slopes (siltstone bedrock) Plant Description: Two small trees Height: 8-9 ft DBH: 0.75 in Bark: Gray, reddish new growth with lenticels Leaves: One tree has reddish fall color, the other has green foliage Fruit: Yellow-green racemes Seed Collected from: 2 plants Herbarium Specimens: 3 Associated Species: Acer rubrum, Cornus florida, Desmodium viridiflorum, Liriodendron tulipifera, Rubus sp. (blackberry), Quercus velutina, Sassafras albidum, Quercus montana, Rhus copallinum Collectors: T. Boland, J. Campbell, P. Douglas, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: 2016-89 38

Field Collection Number: TUCK16-021 Scientific Name: Liquidambar styraciflua Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 827 ft Latitude: 36⁰ 50.198 N Longitude: 84⁰ 20.356 W Habitat Notes: Parking lot of Cumberland Falls State Park Slope: 0⁰ Aspect: N/A Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: One tree (three trunks) Height: 60 ft DBH: 25 in Bark: Gray, striped Leaves: Green, glabrous, star-shaped Fruit: Spiky brown gum ball Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Quercus alba, Fraxinus americana, Liriodendron tulipifera, Platanus occidentalis Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 812-2016 PHA Accession Number: 2016-117 39

Field Collection Number: TUCK16-022 Scientific Name: Itea virginica Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 839 ft Latitude: 36⁰ 50.311 N Longitude: 84⁰ 19.960 W Habitat Notes: On banks of Cumberland River, on side of trail Slope: About 20⁰ down to river Aspect: S Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Shrubs Height: 2 ft DBH: <0.25 in Bark: Green, new growth is red Leaves: Green, lots of foliar damage from insects Fruit: Brown racemes Seed Collected from: Mass of plants Herbarium Specimens: 3 Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 813-2016 PHA Accession Number: 2016-111 40

Field Collection Number: TUCK16-023 Scientific Name: Rhododendron maximum Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 851 ft Latitude: 36⁰ 50.323 N Longitude: 84⁰ 19.941 W Habitat Notes: On trail along banks of Cumberland River Slope: About 10⁰ (slight) Aspect: S Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Large shrub Height: 10 ft DBH: 1 in Bark: Tan, striped, moss and lichen on bark Leaves: Green, waxy, shrub largely defoliated Fruit: Brown capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis, Galax urceolata Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 814-2016 PHA Accession Number: 2016-105 41

Field Collection Number: TUCK16-024 Scientific Name: Galax urceolata Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 851 ft Latitude: 36⁰ 50.323 N Longitude: 84⁰ 19.941 W Habitat Notes: On trail along banks of Cumberland River Slope: About 10⁰ (slight) Aspect: S Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Herbaceous Height: 1 ft DBH: <0.25 in Bark: None Leaves: Green, glabrous, round Fruit: Not recorded Seed Collected from: Mass of plants Herbarium Specimens: None Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: N/A PHA Accession Number: 2016-112 42

Field Collection Number: TUCK16-025 Scientific Name: Stewartia ovata Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 851 ft Latitude: 36⁰ 50.323 N Longitude: 84⁰ 19.941 W Habitat Notes: On trail along banks of Cumberland River Slope: About 10⁰ (slight) Aspect: S Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Multi-stemmed tree Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, slightly lustrous, ovate Fruit: Brown capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis, Galax urceolata Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 815-2016 PHA Accession Number: 2016-90 43

Field Collection Number: TUCK16-026 Scientific Name: Rhododendron maximum Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 859 ft Latitude: 36⁰ 50.329 N Longitude: 84⁰ 19.936 W Habitat Notes: Wet draw stream out of mountain Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Shrub Height: Not recorded DBH: Not recorded Bark: Tan, striped Leaves: Green, waxy Fruit: Capsules Seed Collected from: 2 plants Herbarium Specimens: None Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 816-2016 PHA Accession Number: 2016-118 44

Field Collection Number: TUCK16-027 Scientific Name: Rhododendron maximum Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 859 ft Latitude: 36⁰ 50.329 N Longitude: 84⁰ 19.936 W Habitat Notes: Wet draw stream out of mountain Slope: About 45⁰ to stream Aspect: SE Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Shrub Height: 15 ft DBH: 1.5 in Bark: Tan, striped Leaves: Green, waxy Fruit: Capsules Seed Collected from: 1 plant Herbarium Specimens: None Associated Species: Ostrya virginiana, Tsuga canadensis, Magnolia macrophylla, Quercus velutina, Kalmia latifolia, Acer rubrum, Cephalanthus occidentalis, Ilex opaca, Liquidambar styraciflua Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: N/A PHA Accession Number: 2016-118 45

Field Collection Number: TUCK16-028 Scientific Name: Goodyera pubescens Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 857 ft Latitude: 36⁰ 50.338 N Longitude: 84⁰ 19.913 W Habitat Notes: Trailside, banks of Cumberland River Slope: 20-30⁰ Aspect: SW Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: Herbaceous Height: 1 ft DBH: <0.25 in Bark: White stalk Leaves: Mint green leaves with distinct white pattern Fruit: On single spike Seed Collected from: 1 plant Herbarium Specimens: None Associated Species: Tsuga canadensis, Kalmia latifolia, Magnolia macrophylla, Liquidambar styraciflua, Quercus montana, Liriodendron tulipifera Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: N/A PHA Accession Number: 2016-104 46

Field Collection Number: TUCK16-029 Scientific Name: Kalmia latifolia Date: 17 September 2016 County: Whitley Local Name: Daniel Boone National Forest: Cumberland Falls State Park Altitude: 927 ft Latitude: 36⁰ 50.290 N Longitude: 84⁰ 19.786 W Habitat Notes: Wet draw stream down to Cumberland River, along path Slope: Not recorded Aspect: SE Soil Type: Shelocta-Highsplint-Sequoia complex, 30 to 80% slopes, rocky (sandstone/shale bedrock) Plant Description: One shrub Height: 10 ft DBH: 2.5 in Bark: Brown, striped Leaves: Green, glabrous, ovate Fruit: Green Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Liquidambar styraciflua, Rhododendron maximum, Hamamelis virginiana, Amelanchier arborea, Clethra acuminata, Quercus montana, Fagus grandifolia, Acer rubrum Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 817-2016 PHA Accession Number: 2016-119 47

Field Collection Number: TUCK16-030 Scientific Name: Stewartia ovata Date: 17 September 2016 County: McCreary Local Name: Yahoo Falls Altitude: 929 ft Latitude: 36⁰ 46.327 N Longitude: 84⁰ 31.175 W Habitat Notes: Stream side at Yahoo Falls, by loop trail Slope: 40-45⁰ slope to stream Aspect: E Soil Type: Shelocta-Bouldin complex, 30-75% slopes, extremely stony, very rocky (sedimentary bedrock) Plant Description: Single tree Height: 10 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, lustrous adaxial surface Fruit: Brown capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Rhododendron maximum, Liquidambar styraciflua, Clethra acuminata, Acer rubrum, Quercus alba, Tsuga canadensis, Magnolia macrophylla, Kalmia latifolia Collectors: L. Baird, T. Boland, J. Campbell, R. Castlen, P. Douglas, J. Johnston, C. Meholic, M. Patton, T. Rounsaville, J. Zukswert AA Accession Number: 818-2016 PHA Accession Number: 2016-91 48

Field Collection Number: TUCK16-031 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Whitley Local Name: Bark Camp Creek Altitude: 958 ft Latitude: 36⁰ 54.209 N Longitude: 84⁰ 16.947 W Habitat Notes: Trail on side of Bark Camp Creek, off of Daniel Boone Forest Rd 193 Slope: 40-45⁰ Aspect: SW Soil Type: Shelocta-Highsplint-Sequoia complex, 30-80% slopes, rocky (sandstone/shale bedrock) Plant Description: Single tree Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate Fruit: Capsules Seed Collected From: 1 plant Herbarium Specimens: 3 Associated Species: Rhododendron maximum, Tsuga canadensis, Oxydendrum arboreum, Magnolia tripetala, Kalmia latifolia, Liriodendron tulipifera, Ilex opaca, Quercus rubra, Smilax sp., Acer rubrum Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 49

Field Collection Number: TUCK16-032 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Whitley Local Name: Bark Camp Creek Altitude: 973 ft Latitude: 36⁰ 54.266 N Longitude: 84⁰ 16.863 W Habitat Notes: Roadside (by Daniel Boone Forest Rd 193), on top of rock outcrop, near Bark Camp Creek, in Daniel Boone National Forest Slope: Not recorded Aspect: Not recorded Soil Type: Shelocta-Highsplint-Sequoia complex, 30-80% slopes, rocky (sandstone/shale bedrock) Plant Description: Single tree, hanging over rock Height: 15 ft DBH: 1 in Bark: Gray, striped, lichen Leaves: Green, ovate Fruit: Very large fruits! Green capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Tsuga canadensis, Liriodendron tulipifera, Fagus grandifolia, Quercus alba, Hamamelis virginiana, Hydrangea arborescens, Betula lenta Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 819-2016 PHA Accession Number: 2016-92 50

Field Collection Number: TUCK16-033 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Whitley Local Name: Bark Camp Creek Altitude: 950 ft Latitude: 36⁰ 54.355 N (Jenna doesn t trust this value) Longitude: 84⁰ 15.750 W (Jenna doesn t trust this value) Habitat Notes: Roadside of Daniel Boone Forest Rd 193 Slope: Not recorded Aspect: E Soil Type: Shelocta-Highsplint-Sequoia complex, 30-80% slopes, rocky (sandstone/shale bedrock) Plant Description: Small grove of trees by Bark Camp Creek Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Capsules Seed Collected from: 6 plants Herbarium Specimens: 3 Associated Species: Acer rubrum, Liriodendron tulipifera, Magnolia macrophylla, Tsuga canadensis, Quercus velutina, Quercus alba, Oxydendrum arboreum Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 820-2016 PHA Accession Number: 2016-93 51

Field Collection Number: TUCK16-034 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Whitley Local Name: Bark Camp Creek Altitude: 1025 ft (Jenna doesn t trust this value) Latitude: 36⁰ 54.483 N Longitude: 84⁰ 16.684 W Habitat Notes: Bark Camp Creek side, down from road in valley, near largest Stewartia ovata Jack Johnston has seen in Kentucky Slope: About 20⁰ Aspect: ESE Soil Type: Shelocta-Highsplint-Sequoia complex, 30-80% slopes, rocky (sandstone/shale bedrock) Plant Description: One tree Height: 15 ft DBH: 1 in Bark: Gray, striped, lichen Leaves: Green, ovate, hairy underside Fruit: Green capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Magnolia tripetala, Tsuga canadensis, Betula lenta, Kalmia latifolia, Rhododendron maximum, Vaccinium arboreum Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 821-2016 PHA Accession Number: 2016-94 52

Field Collection Number: TUCK16-035 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Laurel Local Name: Marsh Branch Rd Boat Ramp Altitude: 1257 ft Latitude: 37⁰ 01.013 N Longitude: 84⁰ 14.274 W Habitat Notes: Marsh Branch Rd, in forest north of power line, lots of Smilax Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: One tree Height: 15 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Green capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Smilax sp., Acer rubrum, Ilex opaca, Sassafras albidum, Liriodendron tulipifera, Quercus sp. Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 822-2016 PHA Accession Number: 2016-95 53

Field Collection Number: TUCK16-036 Scientific Name: Stewartia ovata Date: 18 September 2016 County: Laurel Local Name: Marsh Branch Rd Boat Ramp Altitude: 1228 ft Latitude: 37⁰ 00.894 N Longitude: 84⁰ 14.205 W Habitat Notes: Marsh Branch Rd, in forest north of power line (within 50 yards), abundant Smilax Slope: 0⁰ Aspect: N/A Soil Type: Lily loam, 5 to 12% slopes (sandstone/shale bedrock) Plant Description: Four trees nearby Height: 15-30 ft DBH: 1-3 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Green capsules Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Smilax sp., Acer rubrum, Ilex opaca, Sassafras albidum, Quercus alba, Magnolia macrophylla, Oxydendrum arboreum Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 823-2016 PHA Accession Number: 2016-96 54

Field Collection Number: TUCK16-037 Scientific Name: Stewartia ovata Date: 18 September 2016 County: McCreary Local Name: The Gulf Altitude: 1313 ft Latitude: 36⁰ 50.443 N Longitude: 84⁰ 31.862 W Habitat Notes: On side of trail at the Gulf, relatively open area, relatively common in this area (large Stewartia population), lots of Gaylussacia brachycera Slope: 0⁰ Aspect: N/A Soil Type: Wernock-Sequoia complex, 12 to 25% slopes (siltstone bedrock) Plant Description: Several trees Height: 15 ft DBH: 1.5 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Green capsules Seed Collected from: 12 plants Herbarium Specimens: 3 (from 1 plant) Associated Species: Kalmia latifolia, Oxydendrum arboreum, Acer rubrum, Vaccinium sp., Pinus virginiana, Ilex opaca, Quercus coccinea, Gaylussacia brachycera Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 824-2016 PHA Accession Number: 2016-97 55

Field Collection Number: TUCK16-038 Scientific Name: Stewartia ovata Date: 18 September 2016 County: McCreary Local Name: Louie Tucker Rd Altitude: 1313 ft Latitude: 36⁰ 49.711 N Longitude: 84⁰ 29.144 W Habitat Notes: On side of Louie Tucker Rd, forest sloping towards side road, Disturbed site, has lots of poison ivy Slope: About 45⁰ Aspect: WNW Soil Type: Shelocta-Highsplint-Sequoia complex, 30-80% slopes, rocky (sandstone/shale bedrock) Plant Description: Three trees Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Green capsules Seed Collected from: 3 plants Herbarium Specimens: 3 (from 1 plant) Associated Species: Magnolia macrophylla, Ilex opaca, Acer rubrum, Kalmia latifolia, Pinus echinata, Sassafras albidum, Smilax sp., Quercus montana, Nyssa sylvatica, Fagus grandifolia, Tsuga canadensis, Oxydendrum arboreum, Viburnum acerifolium, Hamamelis virginiana, Carya tomentosa Collectors: T. Boland, J. Johnston, C. Meholic, J. Zukswert AA Accession Number: 825-2016 PHA Accession Number: 2016-98 56

Field Collection Number: TUCK16-039 Scientific Name: Pinus virginiana Date: 19 September 2016 County: Pulaski Local Name: Bauer Rd Altitude: 1073 ft Latitude: 36⁰ 54.336 N Longitude: 84⁰ 27.900 W Habitat Notes: Side of Bauer Rd, disturbed area Slope: 0⁰ Aspect: N/A Soil Type: Sequoia-Wernock complex, 6-12% slopes (shale/siltstone bedrock) Plant Description: Single tree, curved trunk Height: 30 ft DBH: 15 in Bark: Thin, brown Leaves: Green needles, twisted, in bundles of two Fruit: Cones, 2-3 inches long, mostly groupings of two Seed Collected from: 1 plant Herbarium Specimens: 3 Associated Species: Liriodendron tulipifera, Cornus florida, Acer rubrum, Quercus velutina, Sassafras albidum, Quercus coccinea, Juniperus virginiana, Magnolia macrophylla Collectors: T. Boland, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: N/A 57

Field Collection Number: TUCK16-040 Scientific Name: Diospyros virginiana Date: 19 September 2016 County: Pulaski Local Name: Bauer Rd Altitude: 1093 ft Latitude: 36⁰ 55.479 N Longitude: 84⁰ 27.247 W Habitat Notes: On side of Bauer Rd, disturbed roadside area Slope: 0⁰ Aspect: N/A Soil Type: Sequoia-Wernock complex, 6-12% slopes (shale/siltstone bedrock) Plant Description: Single tree Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, alternate, simple, ovate Fruit: Round, yellow-green, some with orange Seed Collected from: 1 plant Herbarium Specimens: 3 (not pressed due to poor quality of leaves) Associated Species: Liriodendron tulipifera, Acer rubrum, Quercus velutina, Quercus montana Collectors: T. Boland, C. Meholic, J. Zukswert AA Accession Number: N/A PHA Accession Number: 2016-99 58

Field Collection Number: TUCK16-041 Scientific Name: Stewartia ovata Date: 19 September 2016 County: McCreary Local Name: Blue Heron Overlook Altitude: 1228 ft Latitude: 36⁰ 40.367 N Longitude: 84⁰ 32.606 W Habitat Notes: Side of Blue Heron Overlook trail, new Stewartia site Slope: About 30⁰, slight dip in trail Aspect: NE Soil Type: Lily-Gilpin complex, 20 to 35% slopes (sandstone bedrock) Plant Description: Small grove of Stewartia, about 6 of them are covered in grape vines Height: 9 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Green and brown capsules Biomass Type: Seed Seed Collected from: 3 plants Herbarium Specimens: 3 (from 1 tree) Associated Species: Hamamelis virginiana, Oxydendrum arboreum, Liquidambar styraciflua, Quercus velutina, Kalmia latifolia, Ilex opaca, Carya tomentosa Collectors: T. Boland, C. Meholic, J. Zukswert AA Accession Number: 826-2016 PHA Accession Number: 2016-100 59

Field Collection Number: TUCK16-042 Scientific Name: Stewartia ovata Date: 19 September 2016 County: McCreary Local Name: Blue Heron Overlook Altitude: 1242 ft Latitude: 36⁰ 40.355 N Longitude: 84⁰ 32.604 W Habitat Notes: On opposite side of trail from TUCK16-041: group of 6 trees Slope: About 10⁰ (slight) Aspect: NE Soil Type: Lily-Gilpin complex, 20 to 35% slopes (sandstone bedrock) Plant Description: Several trees Height: 12 ft DBH: 1 in Bark: Gray, striped Leaves: Green, ovate, hairy underside Fruit: Brown and green capsules, lots of fruit in various stages of maturity, about 2 of 5 ovules have seed in capsules checked Biomass Type: Seed Seed Collected from: 4 plants Associated Species: Carya tomentosa, Acer rubrum, Oxydendrum arboreum, Quercus montana, Nyssa sylvatica, Fagus grandifolia, Cornus florida, Ilex opaca, Kalmia latifolia, Quercus velutina, Quercus alba Collectors: T. Boland, C. Meholic, J. Zukswert AA Accession Number: 827-2016 PHA Accession Number: 2016-101 60

Field Collection Number: TUCK16-043 Scientific Name: Oxydendrum arboreum Date: 19 September 2016 County: McCreary Local Name: Blue Heron Overlook Altitude: 1222 ft Latitude: 36⁰ 40.300 N Longitude: 84⁰ 32.617 W Habitat Notes: Right at Blue Heron Overlook, behind fence facing NE, very exposed site Slope: 0⁰ Aspect: N/A Soil Type: Lily-Gilpin complex, 20 to 35% slopes (sandstone bedrock) Plant Description: Two small trees Height: 5 ft DBH: 0.5 in Bark: Green, lenticels Leaves: Reddish fall foliage Fruit: Green capsules Seed Collected from: 2 plants Herbarium Specimens: 3 (from 1 tree) Associated Species: Amelanchier arborea, Acer rubrum, Liriodendron tulipifera, Vaccinium arboreum, Liquidambar styraciflua, Pinus virginiana, Quercus velutina, Rhus copallinum Collectors: T. Boland, C. Meholic, J. Zukswert AA Accession Number: 828-2016 PHA Accession Number: 2016-102 61