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Fresh-cut Fruit & Vegetables Examples of products Physiology of fresh-cut products Preparation and handling Raw material and quality Marita Cantwell micantwell@ucdavis.edu Postharvest Short Course June 2014 FRESH CUT PRODUCTS DEFINITION "Fresh cut produce" is defined as any fresh fruit or vegetable that has been physically altered from its original form, but remains in a fresh state. It has been trimmed, peeled, washed and/or cut into 100% usable product that is subsequently packaged to offer consumers high nutrition, convenience and value while still maintaining freshness. Not all products in this category are cut into pieces: Leafy salad greens, peeled garlic, individual grapes However, all products in this category are alive and respire Minimally processed, lightly processed, partially processed, pre cut, valueadded 1

Estimated Value-added Produce Sales in Select US Supermarkets*,$4.7 Billion, 2012 *Excludes club stores, supercenters, part of conventional grocery and other alternative formats. Overall Value-Added: Retail is about 40% Food Service about 60% Sources: Estimated by Roberta Cook from various sources. Fresh-cut Vegetables Lettuces: cleaned, chopped, shredded Spinach, leafy greens, washed & trimmed Broccoli & cauliflower florets Cabbage, shredded Carrots, baby, sticks, shredded Celery sticks Onions, whole peeled, slices, diced Potatoes & other roots: peeled, sliced, diced Mushrooms sliced Jicama, Squash, cucumber slices, dices Garlic, fresh peeled, slices Tomato and pepper slices In 2012 Bolthouse bought by Campbells 2

Simple lettuce to meal salad Pear Gongonzola Kit Single To 3 component http://www.freshexpress.com Romaine lettuces, green leaf lettuce and red leaf lettuce, lolla rosa, green tango lettuce, green oak leaf lettuce and red oak leaf lettuce, arugula, mizuna, tatsoi, baby spinach, radicchio, dried pears, frosted almonds, and pear gorgonzola vinaigrette. Ingredients may vary. Sweetpotato for Food service Many garlic products Reduced prep timecleaned and trimmed Open bag and add seasonings Reseal (zip lock) and microwave 2011 PMA Product Innovation Award Strips taro, celeriac, sweetpotato Steam in pouch Brussel sprouts and asparagus 3

Prepared Vegetables for Home Cooking/Grilling Most of snacking is NOT from fruits/vegs Opportunities for Fresh-cut/fresh prepared Fruits & Veggies From Snacking in America, NPD report, 2013 4

U.S. Supermarket Fresh-cut Fruit Category Shares (% of sales) Fruit Mix 21.1% Melons 21.6% Apples 18.8% Mango 2.2% Other 3.4% Trays 18.4% Pineapple 14.6% 2009. Source: Roberta Cook; Perishables Group Examples fresh-cut fruit products 5

Lettuce Salad Preparation Harvest Trim, core, defect removal Cool and/or MA Dump, mechanical cut Cooling, disinfection Drying, centrifugation Component blending Weigh and package Metal detector, pack, palletize Temporary cold storage 6

Trend to automated lines Minimize personnel at low temperatures Lower temperature for products 3 separate areas of cleanliness Many large volume (lettuces) products are mechanically cut, but manual preparation generally results in superior quality cutting romaine by hand; eliminate defects manually peeled garlic vs compressed air peeled broccoli and cauliflower florets manually trimmed manually trimmed and cut melons, pineapples 7

Fresh-cut Products Food Safety Requirements Meticulous cleanliness of equipment, employees and product Constant monitoring of sanitizer activity Rigid maintenance of refrigerated temperatures Complete integrity of packages Strict adherence to product use by dates & handling instructions SANITARY PLANT DESIGN: Product, People, Maintenance Focus: Implementation of GMP s Design efficient, easy to clean & sanitize processing plants Mobility of equipment 2-3 areas to separate production steps Vision systems for defect removal Quick release belts for cleaning Cutting systems Rudi Groppe; http://www.heinzen.com/products/ 8

BABY LEAF LINE Sanitation vs Production What is CLEARLY wrong here? Maintain Quality & Safety of Fresh-cut Vegetable Products Use highest quality raw material Minimize mechanical damage; sharp knives Rinse cut surfaces; remove excess water Maintain strict sanitation; chlorinated water Use appropriate package and atmosphere Maintain product temperature at 1-2 C 9

Effects of Fresh-cut Processing Physical Mechanical shock, remove protective layers Cell fluids on cut surface, gas diffusion Exposure to microbial and chemical contamination Physiological Increased respiration, ethylene rates Increases in other biochemical reactions Discoloration and Color Texture Aroma and Flavor Nutritional quality Main strategy to minimize changes is to use low temperature Product cooled before cutting Product prepared in cold room Abrasion peeling of carrots leads to fragmented cell walls that dry out and result in white blush ; can rehydrate carrots. New equipment peels and then cuts the carrots; have less problem with white blush Diagrams from Saltveit, UC Davis 10

Respiration (µl CO2. g -1 h -1 ) 12 Yellow Onion 11 10 9 8 7 6 5 4 3 2 1 0 Intact Low Peeled temperature Diced minimizes wound response Diced onions discolor, decay, soften and lose fluid more readily than whole peeled onions 0 C 5 C 10 C Storage Temperature Respiration rate (µl CO 2.g -1 h -1 ) 140 120 100 80 60 40 20 0 Green Onions Intact Chopped, Manual Chopped, Commercial 0 C 32 F 5 C 41 F 10 C 50 F Temperature 15 C 59 F 20 C 68 F More cutting, higher respiration rates Low temperature minimizes respiration Dices discolor, decay, soften and lose fluid more readily than whole peeled onions Wounds induce phenolic metabolism Leading to unsightly brown pigments Enzymatic Browning PAL Phenylalanine cinnamic acid other phenolics PPO + O 2 (o-diphenol oxidase) (Laccase) Complex brown polymers quinones PAL = phenylalanine ammonia-lyase PPO = polyphenol oxidase 11

Discoloration Rating Scale for Romaine 1=none 2=slight 3=moderate 4=mod. severe 5=severe Impact of Temperature on Broccoli Floret Quality 9 A. Visual Quality cv Marathon 130 B. Floret Color 8 9=excellent, 1=unusable 7 6 5 4 3 2 1 LSD.05 0 C 32 F 2.5 C 36 F 5 C 41 F 7.5 C 45 F 10 C 50 F Color, Hue 120 110 100 90 80 LSD.05 0 C 32 F 2.5 C 36 F 5 C 41 F 7.5 C 45 F 10 C 50 F 118 C. Cut end Color 21 20 D. Total Sugars 116 19 Color, Hue 114 112 110 LSD.05 mg/g fresh weight 18 17 16 15 14 LSD.05 108 13 0 3 6 9 12 15 18 Days 0 3 6 9 12 15 18 Days MA to retard browning Cantwell, UC Davis 12

Prevention of enzymatic/oxidative browning Refrigeration (slows enzymatic reactions) Exclusion of oxygen (CA, MAP, edible films) Inhibition of PAL (lettuces & vegs) Inhibition of PPO (fruits) Use of reducing agents (ascorbic acid, etc.) Other chemical agents Examples of benefit of MA and chemical treatments Romaine 1 Romaine 2 AIR 6 days at 5 C MA (0.7%O 2 + 8%CO 2 ) C.A.+A.A. =citric acid + ascorbic acid 13

Blade and waterjet cutting Minimize damage at cutting and minimize discoloration 1=none, 2=slight, 3=moderate 3.5 3.0 2.5 2.0 1.5 Discoloration Used and resharpened knives New knives Foodgrade nozzle, fast, 35k psi Foodgrade nozzle, slow, 55k psi LSD.05 1.0 0 2 4 6 8 10 12 14 16 18 20 Days at 2.5C Cantwell et al., 2012 Package, no MA; 8 days at 2.5C in air Packaging is a key enabling technology Resealable bags Single serve Snack packs Steam in Bag Rigid containers and bowls Active packaging Intelligent/Smart Packaging Biobased packaging PLA resin 14

Fresh-cut Packaging Bags (LDPE) with holes No modified atmosphere Bags with microperforations Often PP Permeability of O2:CO2 =1:1 Differentially permeable films Layered plastics Co-extruded plastics Gas permeability ratios vary Permeable patches Effect of O2 on Respiration Often lower O2 and increase CO2 A too extreme atmosphere will lead to fermentation Temperature abuse leads to undesirable change in atmosphere An inappropriate atmosphere is worse than no modified atmosphere Effect of CO2 on Respiration 15

Temperature Effects on Retail Packaged Salads Percent 0.20 0.15 0.10 0.05 Oxygen 0 C 32 F 5 C 41 F 10 C 50 F 15 C 59 F 20 C 68 F µl/kg 120 100 80 60 40 Acetaldehyde 9=excellent, 3=poor 9 8 7 6 5 Visual Quality 20 4 Percent 0.00 0 3 25 Carbon dioxide Ethanol Off-odors 3000 5 20 2500 4 15 2000 1500 3 10 1000 2 5 500 1 0 0 0 4 8 12 16 20 24 0 4 8 12 16 20 24 0 4 8 12 16 20 24 µl/kg Days Days Days Peiser and Cantwell, UC Davis 1=none, 5=severe Cut Vegetable Quality and Modified Atmospheres grape tomato celery sticks baby carrots broccoli florets sugar snap peas Vegetable trays - want 18 day shelf-life Products in tray and compatibility issues - raw material sourcing and handling before prepare - shelf-life of individual products in tray varies - temperature; 5 C too low for grape tomatoes - modified atmospheres not good for all products in tray 16

Lettuce Salad Quality Parameters Fresh appearance No decay No discoloration Crisp texture Good aroma and flavor Good nutritional value To date, these quality components have been undervalued Current package atmospheres cause loss in all three Cut Lettuce Alternatives Intact Baby size Clean Whole leaves Hearts of romaine Small mixed lettuces Organic Spring Mix Rely on temperature Control; No MA used 17

MA not allowed on mushroom, Clostridium botulinin risks Chilling sensitive produce needs to be held at low temperature once cut 18

Relative Importance of Temperature and Modified Atmospheres for Fresh-cut melon 7.5 C (45 F) 10 days 5 C (41 F) 2.5 C (36 F) Air 1% O2 Air + 10% CO2 1% O2 + 10% CO2 IMPORTANCE OF TEMPERATURE Commercial prepared red onion rings and dices stored 9 days. Notice the quality at 0 C 0 C 5 C 10 C 19

0 C (32 F) 5 C (41 F) 10 C (50 F) 8d 12d 16d Temporary Storage, Transport and Distribution TEMPERATURE TEMPERATURE TEMPERATURE Good temperature control throughout distribution Temperatures increase during distribution Vertical air flow; ice is a problem What is the temperature of this product? 20

Products Potential post-cutting storage life at 2 to 5ºC VEGETABLES Days Baby carrots, peeled onions, peeled garlic >21 Lettuce salads, whole lettuce leaves, mixes small leaves 14-18 (spring mix, mesclun),spinach leaves, peeled potatoes; sliced root mixtures Broccoli & cauliflower florets, shredded cabbage, shredded 10-14 lettuce, shredded broccoli stem, celery & carrot sticks Pepper and tomato dices, cucumber slices, squash slices, 4-9 mushroom slices, jicama sticks FRUITS Apple wedges, pineapple chunks, pomegranate arils 10-14 Strawberry slices, melon chunks, mango cubes, citrus 2-9 segments, kiwi, peach & pear slices, grape berries For quality and shelf-life: All cut products benefit from low temperature, some benefit from modified atmospheres, and a few benefit from additional treatments Flavor and nutritional life is about 2/3 shelf-life (appearance) Applies to whole products Applies to fresh-cut products Problem: processors and handlers of freshcut products focus too much on shelf-life, not enough on flavor quality But we need more critical data! 21

Quality of Fresh-Cut Fruits and Vegetables Standardization and Inspection There are no U.S. grade standards for fresh-cut products; raw product standards apply; http://www.ams.usda.gov The following booklet provides guidelines and definitions for inspection: USDA. 1998. Fresh-cut produce: shipping point and market inspection instructions. Fresh products branch, Fruit & Vegetable Division, Agricultural Marketing Service, U.S. Department of Agriculture, Washington, D.C. This publication is currently being updated Best if used by dates Factors affecting the nutritional quality of fresh-cut products 1. Genotypic variation 2. Preharvest factors 3. Maturity and ripeness stage 4. Fresh-cut operations 5. Storage conditions Generally, post-cutting life based on visual quality ends before significant losses of nutritional content occur (Gil and Kader) 22

High Quality Raw Material is Necessary for High Quality Fresh-cut Product Brassicas have higher respiration rates than lettuces. Freshness of color and flavor ingredients extremely important. Raw material quality Some important factors-not always known or controlled Cultivar selection Nitrogen, fertilization Water, irrigation Climate and season Maturity Damage on commercial washed and packaged spinach (cv Space) 23

It is hard to underestimate the importance of varieties Baby Carrots and Variety Selection Uniform, bright orange color Small or no core High sugars with no harshness/bitterness Smooth exterior to minimize peeling loss No green should or green core problems Strong tops for mechanical harvest Balance between juicy texture and resistance to shatter Immature IMPORTANCE OF VARIETY Fresh-cut Peach & Nectarine Romaine 1 Romaine 2 Mature Romaine 1 Romaine 2 Overmature 4D 5 C Air Next Level Fresh Fruit Cuts, a division of Fruit Dynamics, Inc., Fresno, CA, has announced that after 5 years of product development, they have identified the proper cultivars, processes and packaging necessary to commercialize fresh cut peaches and nectarines, in many cases with a shelf life exceeding 15 days. 400 varieties evaluated Flavor profile Resistance to discoloration Shelf-life requirement http://www.californiafarmer.com/story.aspx/ fresh/cut/peaches/go/commercial/9/41540; Aug 2010 24

Fresh-cut Fruit Challenges Labor Intensive Production High Cost per pound-yields and price Fruit availability Sourcing domestic and offshore Storage history Stage of ripeness ideal for cutting Maturity at harvest Stage of ripeness; texture Perishable cut product Flavor softening, browning microbial Flavor quality Fresh-cut tomato for food service Shelf-life vs quality Importance of initial ripeness Importance of ripening conditions Color Texture Composition-flavor Difference in juice purge of 2 tomato cultivars 25

High Quality Fresh-cut Products Maintain Quality and Safety Highest quality raw material Minimal cutting damage Meticulous sanitation Low temperature always and MA if needed Less shelf-life for better flavor 19 th Annual The Magazine for Value-Added Produce http://www.freshcut.com/ Annual guide to suppliers equipment, etc. Tuesday-Thursday September 23-25, 2014 At the Buehler Alumni & Visitors Center 3rd International Conference on Fresh Cut Produce September 13-17, 2015 UC Davis http://postharvest.ucdavis.edu http://postharvest.ucdavis.edu 26