Dr. Stefanos D. Koundouras Assistant Professor

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Dr. Stefanos D. Koundouras Assistant Professor Aristotle University of Thessaloniki Faculty of Agriculture, Forestry and Natural Environment School of Agriculture Laboratory of Viticulture University campus 541 24 Thessaloniki Greece T +30 2310 998650 F +30 2310 998665 M +30 6947 802931 Email: skoundou@agro.auth.gr skype: koundouras.stefanos

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

In ancient Greece, wine was an important part of everyday life. Ancient Greeks loved to organize intellectual gatherings called "symposia" where they would eat and talk about philosophical subjects while drinking wine. In these events, wine was served by "oenochooi" (Greek for sommelier). Because wine was mixed with water before consumption, special mixing containers where developed, the "kraters". Wine was then decanted into "oenochoes" and served in drinking cups, the most common were the "kantharos" and "kylix". krater oenochoe kantharos kylix

Wine trade was important between Greek city-states but it was also exported to Southern Europe. Greeks sealed and traded their wines throughout the ancient world inside "amphorae". A wine legislation was also developed in many cities in order to protect the producers from fraud. Ancient Greeks also realized the important influence of the local ecosystem on the characteristics of wine and even created their own Appellations of Origin: Arioussios Oinos from the island of Chios, Thassios from Northern Greece, Mendeous Oinos from Mende of Chalkidiki, Phliassios Oinos in Peloponnese and Maronian Oinos from Thrace.

NAOUSSA First written testimonies of Xinomavro wines produced in the area of Naoussa date to the 16 th century. Naoussa wines were known since the 17 th century. According to Cousinery: Le vin de Naoussa est dans la Macédoine ce que le vin de Bourgogne est en France. Later (1826) Pouqueville documents: These are thick, acidic, tannic wines, consumed after 4-5 years from production, considered to be among the best of the Ottoman Empire. By the end of the 19th century, the wine from Naoussa was exported to Central and Eastern Europe, Egypt and Eastern Mediterranean. In 1913 Naoussa had 1.200 ha of vines.

Barrels containing authentic Naoussa wine had the label of the community of Naoussa and were sealed with wax. Decline of the Naoussa vineyard after the outburst of phylloxera, the Balkan and World Wars and the introduction of new cultivations. Creation or recognition of the PDO Naoussa region in 1971 leads to the revival of the Naoussa vineyard.

AMYNDEON Amyndeon took his name from king Amyndas the 4 th, cousin of Alexander the Great. Archeological excavations discovered an ancient village north of Lake Petres that dates from the Iron Age. The first organized town was reported around the mid-18 th century, when people from the nearby mountains moved to the fertile plains surrounding the lakes. Amyndeon was established as one of the most important commercial centers of Western Macedonia by the end of the 19 th century.

Winemaking in the area of Amyndeon dates to the 3 rd century BC (wine produced in the ancient city of Kella was particularly famous). Wine production also continued during Turkish occupation and at the end of the 19 th century. The wine of the Amyndeon area was highly praised in the early 20 th century and was exported to Monastiri, Belgrade and Vienna. Revival of the Amyndeon vineyard after the Second World War, when the winery of the Union of Agricultural Cooperatives of Amyndeon was created (1959). Remained neglected until the creation of PDO zone in 1972 which led to the revival of the Amyndeon vineyard. A red dry wine and a rose sparkling wine were designated by the Greek VQPRD legislation and, in 1995, a rose (still) wine became the only PDO Greek rose wine.

GREEK VITICULTURE STRUCTURE Area under vines: 120.000 ha Winegrapes area: 70.000 ha Wine production: 3.000.000 hl High fragmentation of vineyards properties: 31.4 % are lower than 0.2 ha 64.0 % are lower than 0.5 ha Over 2 ha are 11,780 exploitations, or 7,000 out of 100,000 grape growers (8% of the total). Only 50 properties are over 9 ha, which is the French average. Variety of ecosystems (mainly mountainous, coastal, island).

GREEK GRAPE VARIETIES More than 300 indigenous varieties are cultivated in Greece Variety Area (ha) (%) 1. SAVATIANO 12,641 17.80 2. RODITIS 9,742 13.72 3. AGIORGITIKO 3,637 5.12 4. XINOMAVRO 2,389 3.36 5. LIATIKO 2,112 2.98 6. CABERNET SAUVIGNON 1,743 2.45 7. ASSYRTIKO 1,704 2.40 others 37,038 52.16 TOTAL 71,010 100.00

PDO for Red Dry Wines in Greece PDO Amyndeon Florina, Macedonia (cv Xinomavro) PDO Archanes Irakleio, Crete (cv Kotsifali, Mandilaria) PDO Goumenissa Kilkis, Macedonia (cv Xinomavro, Negoska) PDO Daphnes Irakleio, Crete (cv Liatiko) PDO Limnos North Aegean (cv Limnio) PDO Messenicola Karditsa, Thessaly (cv Mavro Messenicola, Syrah, Carignan) PDO Naoussa Imathia, Macedonia (cv Xinomavro) PDO Nemea - Corinth, Peloponnesus (cv Agiorgitiko) PDO Paros Cyclades (cv Mandilaria, Monemvasia) PDO Peza Irakleio, Crete (cv Kotsifali, Mandilaria) PDO Cotes de Meliton Chalkidiki, Macedonia (cv Limnio, Cabernet-Sauvignon, Cabernet franc) PDO Rapsani Larissa, Thessaly (cv Xinomavro, Krassato, Stavroto) PDO Rodos Dodecanese (cv Mandilaria) PDO Siteia Lassithi, Crete (cv Liatiko, Mandilaria) PDO Candia Irakleio, Crete (cv Kotsifali, Mandilaria)

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

Xinomavro is the main and noblest indigenous red grape variety of northern Greece (one of the four flagship Greek grape varieties). Its name reveals its basic features: high in acidity (Xino-, sour or acid), red skin color (- mavro, black), with powerful tannins. Medium sized clusters of variable density (depends on vine vigor and clone), medium to high berry size. It ripens in late September to mid-october. Mutates easily (high genetic variability): clonal selection has identified 5 clones.

LOCALISATION Dominates Northern Greece: It is grown in Central and West Macedonia, in North Greece. Main PDO areas: Naoussa and Amyndeon, But also Rapsani and Goumenissa. Grown also in many other areas such as: Velventos, Kozani, Siatista, Gianitsa, Trikomo, Ossa etc. Amyndeon Naoussa

Krassato XINOMAVRO BLENDS With Negoska (PDO Goumenissa) or Krassato and Stavroto (PDO Rapsani) Xinomavro is also blended with Merlot and Syrah, which round-out and provide depth and complexity to Xinomavro wines. Stavroto Naoussa Amyndeon Goumenissa Rapsani Negoska

THE VINE Fickle and difficult to grow, Xinomavro is a very vigorous and productive variety. Sensitive to warm weather and drought. Sensitive to powdery and downy mildiou and botrytis. Sensitive to potassium deficiency (leading to high acidities, often > 7.5 g/l). Quality of ripening depends greatly on climate (spatial and temporal variations) and soil conditions. Canopy management practices are very important for grape attributes (vigor control cluster microclimate). Yield control very important.

FLAVOR PROFILE Fruity: strawberry, sour cherry Vegetal: tomato, olive, mushroom, tobacco, tar Aged: dried plum, fig, dried tomato, olive pate, truffle. PHENOLIC PROFILE The wines have pale to purplish-red color and characteristic dry, chalky and angular tannins. This combination of high acidity and phenolic richness results in wines with the capacity to age for many years, sometimes decades: the highest ageing potential of all Greek wines.

Pinot noir: pale, fast developing color, high acidity, but more exotic red-fruit nose. Nebbiolo: similarly firm structure and high acidity, together with a similar truffle-like, earthy character. However, Xinomavro is unique: Pinot noir Nebbiolo Idiosyncratic and authentic aromatic profile (tomato, olive) Rare: only 2,000 hectares producing about 85,000 hl/year, in a very specific area (NW Macedonia, North Greece) Versatile: red, rosé, even blanc de noir, still and sparkling, dry and sweet wines.

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

One of the world s most difficult grapes to grow Sensitive to suboptimal conditions and disease outbreaks, under the right conditions it can give wines of unique character. In traditionally made Xinomavro wines, the character of tomato and cabbage is exacerbated, the characteristic tannic and acid palate is persistent. Also, oak ageing decreases color intensity and advances the pale hue of the wines.

PHENOLIC RIPENESS IMPORTANT * Seed ripening monomers Seed ripening procyanidins * - During ripening, seed tannins decline due to an oxidative procedure. - Seed tannin extractability is decreased during ripening..

HARVEST TIME IMPORTANT XINOMAVRO s AVERAGE MATURITY DATES (asynchronous ripening) 09/29 09/25 10/01 10/04

GROWING XINOMAVRO IS A CHALLENGING TASK Only the selection of sites with distinct soil and climate characteristics, and the application of appropriate viticultural and winemaking techniques, lead to the production of wines with distinguished characters: complex wines with deep color, riper tannins and long ageing potential, early-drinking, fruity, elegant wines with lower acidity and tannins.

SITE SELECTION Vine cycle and regional climate must be correctly matched (both Amyndeon and Naoussa) Hot year 9/20 Normal year 9/30 Cool year 10/15 Growth season millesime Xinomavro cycle Full ripening <variations due to year>

Κλίµα mesoclimate Cool Climate In most wine producing countries, correct match corresponds to harvest situated between 15 September and 15 October (mild climatic conditions) for optimum aromatic and phenolic ripening (slow ripening process): Pinot noir in Burgundy Riesling in Alsace Merlot in Bordeaux Sangiovese in Tuscany Nebbiolo in Piemonte Tempranillo in Rioja Xinomavro in Amyndeon and Naoussa

VINEYARD MANAGEMENT Permanent ground cover Vine balance low to moderate vigor low to moderate yield Mainly by - low fertility soils - deficit irrigation - limited fertilization - ground cover - bunch thinning. Green harvest around veraison

VINEYARD MANAGEMENT Xinomavro is particularly sensitive to drought stress. Mild water deficit is desirable but water supply should be enough to allow a steady ripening process (i.e. regulated deficit irrigation RDI). Budburst Ψs (bar) Απουσία No deficitstress 5 Light Μικρό deficit stress 8 Mild Ήπιο deficit stress 11 Moderate Μέτριο stress deficit 14 Έντονο Severe deficit stress Berry set Veraison Harvest Leaf fall

VINEYARD MANAGEMENT Grape microclimate Adequate light penetration in the cluster zone Mainly by - slope aspect - trellis system - row orientation - leaf stripping. Guyot training system Late season leaf removal

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

The long history of Xinomavro cultivated in regions with different soil, climate and viticultural techniques resulted in great genetic variability with respect to its morphological and technological characteristics. Goumenissa Rapsani Different biotypes between areas of cultivation Amyndeon Naoussa

CLONAL SELECTION OF XINOMAVRO Dr. Haroula Spinthiropoulou Clonal selection procedures started in 1990 including a) marking of existing biotypes grown in different Xinomavro cultivation areas, b) virus detection (ELISA, multiplex nested RT-PCR and indexing), c) genetic identification and discrimination, using molecular methods (RAPD-PCR), d) establishment of a study collection of all virus-free biotypes (clones), e) enological evaluation, f) installation of experimental vineyard for comparative agronomic and enological evaluation and g) multiplication of the most interesting clones.

Xinomavro clones present significant differences in most vine and grape attributes (cluster and leaf size and shape, berry color and size etc.) as well as in wine characteristics. Only five (V1, V2, V3, V6, V11), out of 20 initially selected biotypes with stable characteristics, seem to bear distinct enological features and fulfill the requirements to be recognized as clones of Xinomavro, with the most interesting being V3 and V6. Table 1. Yield and must composition of the 5 Xinomavro clones Clone V1 V2 V3 V6 V11 Yield (kg/vine) Potential Alcohol (%) ph 5 4.8 5.5 5 5.5 11.5 11.8 12.9 12.7 11.8 3.65 3.64 3.55 3.56 3.61 Table Titratable 2. Tasting Acidity evaluation (g/l) of the wines 6 of the 5 Xinomavro 6.5 clones 7.3 7.3 6.3 Clone General Impression Ageing Potential V1 15 8 V2 14 10 V3 18 13 V6 17 14 V11 15 10

Xinomavro most important clones V3 Red fruit aroma V6 Typicity (tomato, olive) Population - Biodiversity 234

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

Naoussa Latitude 40-41 o N The viticultural zone of Naoussa lies at an altitude ranging between 350 to 1,500 ft at the foot of Mount Vermion in Central Macedonia. East-facing slopes mainly The climate is Mediterranean with continental influences. Mount Vermion offers protection from cold winds and adequate rainfall.

The present-day PDO Naoussa vineyard has a total area of about 500-600 ha and about 20 wineries. 100-120 ha of other varieties (mainly Syrah, Merlot but also Roditis, Prekniariko etc.) Vines used to be trained in gobelet in dense plantations. Now, cordon-trained, spur-pruned vineyards with approximately 3000-4000 vines per ha. Main rootstocks are 110 R and 41B (140Ru and 1103P were recently introduced). 90% of the vineyards are irrigated. 20% under organic farming. Budburst in early April, harvest in late September to mid October (harvest spans across 3 weeks in the whole region) Legal limit of PDO is 70 hl/ha (half is required for quality wines)

NAOUSSA PDO MAIN XINOMAVRO GROWING AREAS AMYNDEON Mount Vermion (6,735 ft) NAOUSSA Strantza Yianakohori Ramnista Marina Polla Nera TO EDESSA " Gastra Fytia Stenimachos Trilofos TO VERIA

6 CLIMATIC ZONES Zone 1 2 3 4 5 6 Altitude(ft) 400 500 700 600 900 1,100 Aspect E-NE E-NE S-SE S-SE S-SE NE-SE Huglin Index (Apr Sept) 2,715 2,630 2,610 2,585 2,430 2,350 Average Season Temperature (Mar Oct) ( C) 19.40 19.90 18.80 18.90 18.30 18.00 Mount Vermion (6,735 ft) No days with T>30 54.6 52.6 46.5 46.3 39.9 35.3 Rainfall (Mar Oct) (in) 10.5 9.5 9.8 9.8 9.6 14.0 NAOUSSA 3 Strantza 5 Yianakohori Ramnista Marina 4 Polla Nera Gastra 2 Fytia 6 1 Stenimachos Trilofos

5 MAIN SOIL TYPES Best wines with ageing potential are associated with calcareous clay soils leading to good drainage and root aeration and medium vine vigor. Κ1: Loamy soils (SCL, L), without CaCO 3 (23% of total) Κ2: Loamy soils (SCL, L), with CaCO 3 > 4% (15% of total) Κ3: Clay soils (SC, SL, C) without CaCO 3 (25% of total) Κ4: : Clay soils (SC, SL, C) with CaCO 3 > 4% (30% of total) Κ5: Acidic soils (ph<6) (7% of total) Κ4 Wine attributes K1 K2 K3 K4 K5 Color Dark red Medium red Medium red Dark red Pale red Tomato olive aroma Medium Intense Medium Intense Intense intense intense Dry fruit aroma Intense, Medium Medium Intense Medium elegant intense intense intense Structure and body Average to Average Average to Rich and Low good good balanced Ageing potential Average Average Average High Low

13 different crus inside PDO Naoussa showing significant differences in vine performance and grape attributes Fytia NAOUSSA Trilofos Gastra Strantza Yianakohori Ramnnista Variability in soil and climate results in differences in vine vigor and, hence, in berry weight and composition Average berry weight Average shoot weight

XINOMAVRO CHARACTERISTICS AMONG ZONES At lowest altitude level, in Trilofos-Stenimachos, on deep soils, the harvest is earlier and grapes suitable for light red wines while Fytia is the latest ripening areas at the highest altitude (>1000 ft). The central zone of the PDO region (600 to 800 ft) contains some of the best crus like Stranza, Ramnista and Gastra with trademark wines of tannic Xinomavro wines with ageing potential, mostly on clay soils with high lime percentage (high ph). Ramnista Gastra

Presentation Layout 1 History & Statistics 4 The Clones 2 The Varietal 5 Overview Naoussa 3 Viticulture 6 Overview Amyndeon

Amyndeon Latitude : 40,39 N, at the cooler edge of Greek viticulture. The viticultural zone of Amyndeon lies at an altitude of 1,800 to 2,500 ft in Northwest Macedonia (west of Naoussa). Generally poor, sandy or loamy soils on calcareous bedrock.

The area is dominated by 2 connected lakes, among which lake Vegoritis is the biggest. The ecosystem of the lakes, together with the surrounding mountains of Voras (N), Vitsi (W) and Vermio (SE), is protected by the European network NATURA 2000. The lakes of the area regulate the extremities of the climate (especially during winter). Soils around the lakes unsuitable for viticulture (rich in water reserves and organic matter).

The coldest winemaking zone of Greece. It is a mountainous area with a mild continental climate, with fresh summers (18.3 o C season temperature) and a heat summation lower than 1,600 GDD(comparable to Barolo). Cool night temperatures (lower than 16 o C on average for July and August) Moderately warm daytime temperatures (lower than 30 o C on average for July and August but close to 24 o C during September). Growth season rainfall lower than 6 in. Cool N-NE winds at a speed of 2.5 m/s during summer. Cooler and drier than Naoussa climate. Air temperature (oc) 80 60 40 20 Degree Days 1/4-30/9: 1500-1600 0 1 2 3 4 5 6 7 8 9 10 11 12 month of year

At present, PDO Amyndeon vineyard has a total area of about 650 ha. 250 ha of other varieties (mainly Syrah, Merlot, Roditis, Malagouzia, Sauvignon, Chardonnay, Gewürztraminer etc.) Modern, cordon-trained vineyards along with clusters of old vineyards. Planting densities at 3000-4000 vines per ha. Main rootstock is 41B but 110 R and 1103P are recently introduced (some old, own-rooted, pre-phylloxera vineyards in gobelet still exist). New vineyards are irrigated (small percentage). Budburst in early April, harvest in late September to mid October. New vertically trained vines Old bush vines

AMYNDEON PDO MAIN GRAPE GROWING AREAS TO FLORINA PETRES AMYNDEON Petres lake AGHIOS 750 m = 2460 ft Vegorotis lake VEGORA TO KOZANI " TO EDESSA "

AMYNDEON PDO MAIN SOIL TYPES Soils on alluvial deposits of the lakes. Rich in mineral elements. Aghios

AMYNDEON PDO MAIN SOIL TYPES Soil conditions vary in relation to a) texture of the topsoil and b) depth of the topsoil. The soft calcareous bedrock also provides water during the summer months. Type I: Sandy soils on soft limestone (sand > 85%) - Mainly on slopes near Lake Petres (alluvial soils) - Low fertility - Limited water reserves - Early sites (ideal for aromatic and elegant Xinomavro wines) Type II: Sandy-loamy soils on soft limestone (sand < 70%) - Mainly in plains at lower altitude - Slightly higher fertility and depth - Late sites (ideal for structured and balanced Xinomavro wines). Type I, shallow topsoil Type I, deeper topsoil Type II

AMYNDEON PDO MAIN SOIL TYPES Shallow sandy soils AGHIOS AMYNDEON Deeper sandy loamy soils VEGORA

The high altitude (1,800 to 2,500 ft), the neighboring water formations, and the various soil types create a unique ecosystem were Xinomavro produces a variety of wine styles. PDO AMYNDEON WINE STYLES Amyndeon Xinomavros show a pronounced red fruit and floral character, higher acidity due to altitude and the cooler climate, and an overall leaner mouth than Naoussa (also due to the different clone). 1. Aghios Style (type I soil) Fruity, elegant wines with lower acidity and tannins, to be drunk more easily. 2. Vegora Style (type II soil) More structured wines with deep color, riper tannins and long ageing potential.

TO FLORINA Petres lake Hedgehog Vegorotis lake AMYNDEON Old vines TO KOZANI " TO EDESSA "

The Alpha Estate Vineyard in Amyndeon Παρέµβαση Άγγελος Δοκιµή κρασιών

ALPHA ESTATE is located at the NW part of Greece, on the Amyndeon plateau, Florina region. Alpha Estate was founded in 1997 by the experienced viticulturist Makis Mavridis and chemist-oenologist Angelos Iatridis. ALPHA ESTATE is located in the heart of PDO Amyndeon, at an altitude of 2,000 to 2,350 ft a total area of 850 ha. Main varieties: Xinomavro, Mavrodaphne, Syrah, Merlot, Pinot Noir, Tannat, Montepulciano, Malagouzia, Assyrtiko, Sauvignon Blanc, Chardonnay & Gewürztraminer.

Planting density at 3,900 vines per ha. Vines are double-cordon trained and pruned to 6 spurs of 2 buds. Vines are cultivated according to Integrated Management System. Ground cover with natural vegetation is used to control vine vigor. Irrigation system is installed (subsoil). Irrigation is decided upon water potential measurements according to RDI (Regulated Deficit Irrigation) principles. Average yield 30-100 hl/ha.

HORIZONTAL VINIFICATORS Specially adapted equipment for the selective removal of Xinomavro seeds during maceration process, based on the principle of perforated grill at the bottom of the tank.

Alpha Xinomavro Single Vineyard Hedgehog 2010 PDO Amyndeon. Hedgehog sub-region. 2,400 ft altitude, with northern exposure. Destemming, light crushing, cold soak - skin contact, alcoholic fermentation at gradual increasing temperatures, maintenance of wine sur lies for 8months. 12 months in medium grain French oak & 12 months in the bottle. Bright purple-red color. Complex, typical bouquet of small berry red fruits and spices. Full mouth, rounded tannins, balanced acidity and well integrated wood. Long aftertaste with intense quince aroma.

Alpha Xinomavro Reserve Old Vines 2009 PDO Amyndeon. Over 93 y.o., ungrafted. Gobelet 100% Turtles subregion. Altitude 2,000 ft. Destemming, light crushing, cold soak - skin contact, alcoholic fermentation at gradual increasing temperatures, maintenance of wine sur lies for 8 months. 18 24 months in Allier Bertrange French oak casks & 18 24 months in the bottle. Bright dark ruby color. Complex nose of sundried tomatoes, black fruits, licorice and spices. Full body, with rich fruit depth, blackberries, plums, herbaceous hints and oak on the back. Solid, velvety tannins and long aftertaste with persistent aromas.

Alpha Xinomavro Reserve Old Vines 2006 Extremely cool temperatures in early June delayed bloom. Moreover, the lower than average summer temperatures, combined with frequent rains, prolonged vine cycle leading to steady ripening with high acidities but also high phenolic extractibility in the wine. Malolactic fermentation was particularly beneficial for Xinomavro. Black cherry and blackberry. Firm structure, with textured tannin, some chocolate character and a cedarwood finish, considerable ageing potential.

Alpha Xinomavro Reserve Old Vines 2004 2004 was a very challenging vintage because of the higher than average rainfalls during the ripening period. A rigorous field work allowed us to get the optimum level of ripening. Ground cover achieved limitation of water supply to the vine and provided the right balance for Xinomavro. Higher level of malic acid. We can judge 2004 as one the most fine vintages made in Alpha Estate. Red - brown color. Nose of red fruit, licorice and spices. Light body, with herbaceous hints and oak on the back. Acidity and tannins still prominent. Ready to drink.

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