ANTIOXIDANT ACTIVITY The antioxidant activity of selected medicinal plants was performed at Chemistry Research Laboratory, Deogiri College, Aurangabad. 8.1 Preparation of sample solution About 0.10 g of dry plant powder was accurately weighed in a stopper tube. In stopper tube, 10.0 ml of aqueous methanol (40%) was added and incubated in a incubator for about 24 hrs. The contents of the tube were filtered through Whatman filter paper No. 41 (Merck, India). The filtrate was used for further analysis. 8.2 DPPH radical scavenging assay DPPH (2, 2-dipheny l-2- picrylhydrazyl) radical scavenging assay was carried out as per reported method with some modifications Blois (1958) and Roberta, et al., (2006). About 1 ml of test solution was added to equal quantity of 0.1 mm solution of DPPH in ethanol. After 20 min of incubation at room temperature, the DPPH reduction was measured by reading the absorbance at 517 nm. Ascorbic acid (1mM) was used as reference compound and it has 51.33 ± 1.02 % of DPPH antioxidant assay. Sr. No Plant name DPPH antioxidant assay (%) 1 Cucumis setosus fruit 59.66 ± 1.52 2 Lagenaria siceraria fruit 23.60 ± 1.41 3 Trichosanthes tricuspidata fruit 65.00 ± 1.00 4 Coccinia grandis fruit 72.14 ± 0.52 5 Diplocyclos palmatus fruit 60.33 ± 0.25 6 Cucumis setosus leaf 29.26 ± 0.64 7 Trichosanthes tricuspidata leaf 54.00 ± 1.47 8 Coccinia grandis leaf 18.40 ± 0.78 9 Diplocyclos palmatus leaf 22.33 ± 0.49 10 Lagenaria siceraria leaf 14.85 ± 0.73 Standard Ascorbic Acid 51.33 ± 1.02 Table No 128: Antioxidant activity of selected medicinal plants 178
8.3.1 Antioxidant activity of Cucumis setosus fruit The antioxidant activity of methanol fruit extract of C. setosus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is 59.66 ± 1.52. 8.3.2 Antioxidant activity of Lagenaria siceraria fruit The antioxidant activity of methanol fruit extract of Lagenaria siceraria was The DPPH antioxidant assay percentage is 23.60 ± 1.41 8.3.3 Antioxidant activity of Trichosanthes tricuspidata fruit The antioxidant activity of methanol fruit extract of Trichosanthes tricuspidata was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is 65.00 ± 1.00 8.3.4 Antioxidant activity of Coccinia grandis fruit The antioxidant activity of methanol fruit extract of Coccinia grandis was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is 72.14 ± 0.52 8.3.5 Antioxidant activity of Diplocyclos palmatus fruit The antioxidant activity of methanol fruit extract of Diplocyclos palmatus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is 60.33 ± 0.25 8.3.6 Antioxidant activity of Cucumis setosus leaf The antioxidant activity of methanol leaf extract of Cucumis setosus was examined by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH antioxidant assay percentage is 29.26 ± 0.64. 179
8.3.7 Antioxidant activity of Trichosanthes tricuspidata leaf The antioxidant activity of methanol leaf extract of Trichosanthes tricuspidata was The DPPH antioxidant assay percentage is 54.00 ± 1.47 8.3.8 Antioxidant activity of Coccinia grandis leaf The antioxidant activity of methanol leaf extract of Coccinia grandis was The DPPH antioxidant assay percentage is 18.40 ± 0.78 8.3.9 Antioxidant activity of Diplocyclos palmatus leaf The antioxidant activity of methanol leaf extract of Diplocyclos palmatus was The DPPH antioxidant assay percentage is 22.33 ± 0.49 8.3.10 Antioxidant activity of Lagenaria siceraria leaf The antioxidant activity of methanol leaf extract of Lagenaria siceraria was The DPPH antioxidant assay percentage is 14.85 ± 0.73 The DPPH free radical scavenging activity of standard reference ascorbic acid was 51.33 ± 1.02. In present study the DPPH free radical scavenging activity of fruit was more than that of reference standard ascorbic acid. Antioxidant analysis of present study reveals that the maximum DPPH antioxidant assay percentage was observed in Coccinia grandis fruit 72.14 ± 0.52, Trichosanthes tricuspidata fruit 65.00 ± 1.00, Diplocyclos palmatus fruit 60.33 ± 0.25 and Cucumis setosus fruit 59.66 ± 1.52 which was more than that of standard ascorbic acid. The Trichosanthes tricuspidata leaf has DPPH free radical scavenging activity 54.00 ± 1.47 which also more than that of standard ascorbic acid. The results were dedicated in Table No 128 and Graph Nos 11& 12). 180
60 Plant Part Ascorbic Acid 50 40 30 20 10 0 Cucumis setosus Trichosanthes tricuspidata Graph No: 11 Coccinia grandis Plant Part of leaf Diplocyclos palmatu Lagenaria siceraria Antioxidant activity of leaf of selected plants 181
Chapter 8: Antioxidant Activity Plant Part Ascorbic Acid 80 70 60 50 40 30 20 10 0 Cucumis setosus Lagenaria siceraria Trichosanthes tricuspidata Plant Part of fruit Coccinia grandis Diplocyclos palmatus Graph No :12 Antioxidant activity of fruit of selected plants 182