20150310g.pdf IR4 Ornamental Horticulture Program Research Report over Sheet Researcher(s) Gary hastagner Trial: 2014 WA Affiliation: Washington State University Pullayup WA PhoneNumber: (253) 4454528 Email: chastag@wsu.edu ProjectTitle: Botrytis Efficacy Protocol #: 14001 PR# Research Target rop/plant Product EPA Reg. # Production Site Status 32070 Botrytis Gray Mold Alibi Flora (A13703G) S Difenconazole + Azoxystrobi Syngenta Field ontainer 31355 Botrytis Gray Mold Badge X2 opper Oxychloride + opp 8028912 IsaGro Field InGround 31935 Botrytis Gray Mold BAS703 06F/BAS703 01F Fluxapyrosad + pyraclostrobi BASF Field ontainer 32253 Botrytis Gray Mold F9110 F9110 FM Field ontainer 32259 Botrytis Gray Mold Medallion Fludioxonil 100769 Syngenta Field ontainer 32071 Botrytis Gray Mold Mural (A18126B) WDG Benzovindiflupyr + Azoxystro Syngenta Field ontainer 31530 Botrytis Gray Mold Pageant 38WG Boscalid + Pyraclostrobin 7969251 BASF Field InGround 32260 Botrytis Gray Mold Prestop Gliocladium catenulatum Str 6413711 AgBio Field ontainer 32254 Botrytis Gray Mold Proud 3 Thyme oil (5.6%) Biohumanetics Field ontainer 32080 Botrytis Gray Mold Regalia O5 (MOI10605) Extract of Reynoutria sachali 840593 Marrone Bio In Field InGround 31936 Botrytis Gray Mold S2200 4S Mandestrobin Valent Field ontainer 32255 Botrytis Gray Mold SP2770 10WP SP2770 SePro Field ontainer 32256 Botrytis Gray Mold SP2773 SP2773 SePro Field ontainer 32257 Botrytis Gray Mold Torque 3.6S Tebuconazole 100187 leary Field ontainer 31937 Botrytis Gray Mold ZeroTol Hydrogen dioxide 702991 BioSafe Syste Field InGround
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Narrative Summary (Results/Discussion) A total of 15 products were evaluated for their effectiveness in controlling Botrytis spp. on containergrown oral Sunset peonies. Very little disease developed on the plants during this trial. Initial isolations taken from diseased plant material on April 23, 2014 confirmed the presence of B. cinerea and a newly identified species (G3PDH) which is similar to B. calthae. Isolations taken on October 29, 2014 confirmed the presence of Botrytis as well as other pathogens such as ladosporium, Rhizoctonia and Alternaria. Although average disease severity ranged from 0.8 to 5.0, there was considerable variability within the data and none of the treatments affected disease development (Table 2). Based on overall quality ratings, none of the fungicides delayed senescence of the plants compared to the nontreated checks (Table 2). Badge X2, Pageant, Mural, Medallion and both SP numbered products had significantly higher residue ratings than nontreated checks (Table 2). No phytotoxicity was observed in this trial. Plot Layout
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Results Tables Table 1: Treatment list and application dates. Trt # Products PR# Prod/ 100 gal Interval Batch/Lot Number Application Dates 1 1 Noninoculated 2 Inoculated 3 BAS703 01F 31946 8 fl oz 14 day 270037 4 F9110 31522 24 fl oz 7 day D31230713 A 5 Proud 3 31524 4 qts 7 day HG81009252012 A 6 S2200 4S 31936 7.5 fl oz 14 day VT132439 7 SP2770 10WP 31525 2.66 lbs 7 day None provided A 8 SP2773 31527 1.66 lb 14 day None provided 9 SP2773 31526 3.31 lbs 14 day None provided 10 Torque 3.6 S 31527 8 fl oz 14 day 13203VL001 11 Alibi Flora S 32070 14 fl oz 14 day 723374 12 Mural WDG 32071 7 oz 14 day 687643 13 Pageant 38WG 31530 14 oz 14 day 2236S02EJ 14 Regalia 5O 32080 4 fl oz 7 day 106051Z1303B A 15 ZeroTol 31937 1 gal 7 day None provided A 16 Medallion WDG 31534 8 oz 14 day YGM2l19001 17 Prestop 31535 4.2 lbs 21 day W1601 D 18 Badge X2 31355 2 lbs 714 day None provided B 1 Dates: 1 = 4/18/14, 2= 4/25/14, 3= 5/2/14, 4 = 5/8/14, 5 = 5/16/14, 6 = 5/22/14, 7 = 5/30/14, 8 = 6/6/14, 9 = 6/16/14, 10 = 6/23/14, 11= 8/28/14, 12= 9/4/14, 13= 9/11/14, 14= 9/19/14, 15= 9/27/14, 16= 10/7/14, 17 = 10/10/14; A = 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16; B = 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, 17; = 1, 3, 5, 7, 9, 11, 13, 15, 17; D = 1, 4, 7, 10, 11, 14, 17.
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Table 2: Effect of foliar fungicides on the level of residues observed on foliage on May 16, disease severity on September 25, and the overall plant quality on October 27, 2014 1. Trt # Products Prod/ 100 gal Interval Residues Disease Severity Plant Quality 1 Noninoculated 0.0 e 2.4 a 2.4 a 2 Inoculated 0.0 e 1.2 a 3.0 a 4 F9110 24 fl oz 7 day 0.0 e 2.8 a 2.4 a 5 Proud 3 4 qts 7 day 0.0 e 3.0 a 2.0 a 17 Prestop 4.2 lbs 21 day 0.0 e 3.6 a 2.0 a 14 Regalia 5O 4 fl oz 7 day 0.2 de 2.8 a 1.8 a 10 Torque 3.6S 8 fl oz 14 day 0.4 de 1.8 a 2.4 a 11 Alibi Flora 14 fl oz 14 day 0.4 de 0.8 a 2.8 a 15 ZeroTol 1 gal 7 day 0.4 de 2.4 a 2.0 a 3 BAS70301F 8 fl oz 14 day 0.6 de 1.8 a 2.5 a 6 S2200 4S 7.5 fl oz 14 day 0.6 de 2.0 a 2.6 a 12 Mural WDG 7 oz 14 day 1.0 cd 2.2 a 3.2 a 13 Pageant 38WG 14 oz 14 day 1.6 bc 3.6 a 2.4 a 8 SP2773 1.66 lb 14 day 1.8 bc 2.4 a 2.0 a 9 SP2773 3.31 lbs 14 day 1.8 bc 1.6 a 2.2 a 16 Medallion WDG 8 oz 14 day 1.8 bc 2.2 a 2.0 a 7 SP2770 10WP 2.66 lbs 7 day 2.0 b 3.6 a 2.6 a 18 Badge X2 2 lbs 714 day 3.0 a 5.0 a 2.0 a 1 Fungicide residue was rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage. Disease severity was rated on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage was diseased. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, 5 = excellent plants with green foliage. Numbers in columns that are followed by the same letter are not significantly different, P=0.05, Tukey's Studentized Range Test.
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Experiment Information Experimental Design: Randomized omplete Block Number of Reps: 5 Materials & Methods/Recordkeeping Peonies used in this trial were planted in 5 gallon pots in the fall of October, 2013. Plants were blocked based on their height at the start of the trial. They were arranged in a container nursery area in rows that were spaced 4 feet apart with pots spaced 36 apart within rows. The plants were overhead irrigated during the growing season. Treatments initially started on April 18, 2014 and were applied on 7, 14, and 21 day intervals (Table 1). Treatments were delayed starting midjune due to the onset of very warm and dry weather that did not favor disease development. Plants were fertilized on June 9 and July 29, 2014 with 150 ppm of Peter s Professional 201020 water soluble fertilizer. On August 8, 2014 the plants were topdressed with Apex 16611 NPK MAX at a rate of 30 g/pot. The treatments were resumed on August 28, 2014. All treatments were applied in the equivalent of 100 gallons of water and the foliage on plants were sprayed to wet. Each plant received approximately 70.5 ml solution at each application. On August 29, 2014 the plants were inoculated with a spore suspension of and Botrytis paeoniae. Periodically during the growing season, disease development was assessed by rating disease severity on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage was diseased. Isolations were also done from randomly selected symptomatic samples to determine the cause of the leaf spots at the onset of the trial and at the end of the trial. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, and 5 = excellent plants with green foliage at the end of the trial to determine if any of the treatments affected the rate of plant senescence. Fungicide residue was rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage.
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Symptomatic Plants on April 23, 2014
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Name(s) of Personnel onducting Research: Gary hastagner and Annie DeBauw Location of Trial (city/state): WSU Puyallup Research and Extension enter Use Site (greenhouse/shadehouse/field container/etc): ontainer nursery rop History rop ultivar/variety: oral Sunset Date of Planting: October, 2013 Soil Mix: Specialty Soils Professional Growing Media Row spacing 4 Pot spacing: 36 Product(s) applied during experiment (including treatments, fertilizers, etc): PRODUT/ODE PRNUMBERS OMMON NAME MANUFATURER BAS 703 01F 31946 proprietary BASF F9110 31522 extract of Lupinus FM Proud 3 31524 thyme oil BioHumanetrics S2200 4S 31936 mandestrobin BuFarm SP2770 10 WP 31525 proprietary SePRO SP2773 31526 proprietary SePRO Torque 3.6S 31527 tebuconazole NuFarm Pageant 38 WG 31530 pyraclostrobin (12.8), boscalid (25.2) BASF Alibi Flora S 32070 Azoxystrobin (18.2), difenoconazole (11.4) Syngenta Badge X2 31355 copper oxychloride (16.81), copper Gowan hydroxide (15.36) Medallion 31534 fludioxonil Syngenta Mural WDG 32071 Azoxystrobin (30), benzovindiflupyr (15) Syngenta Prestop 31535 Gliocladium catenulatum strain J1446 Verdera Regalia 5O 32080 Extract of Reynoutria sachalinensis Marrone Bio Innovations Zerotol 31937 Hydrogen dioxide, peroxyacetic acid BioSafe Systems Peter s Professional 201020 Scotts APEX NPK MAX 16611 Simplot Professional Products
Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: 14001 PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Photos See BT114; IR4 Botrytis Photos file for various disease symptom photos. Data ollected Initial plant height data was taken on April 18, 2014. Periodically during the growing season, disease development was assessed by rating disease severity on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage diseased. Isolations were also done from randomly selected symptomatic samples to determine the cause of the leaf spots. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, 5 = excellent plants with green foliage at the end of the trial to determine if any of the treatments affected the rate of plant senescence. Visible fungicide residue levels were rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage. Initial isolations taken from diseased plant material on April 23, 2014 confirmed the presence of B. cinerea and a newly identified species (G3PDH) which is similar to B. calthae. Isolations taken from plant material on October 29, 2014 confirmed Botrytis spp. were present as well as other pathogens such as ladosporium, Rhizoctonia, and Alternaria. Data analysis: Data were analyzed using ANOVA (GLM SAS). Treatment means were separated using Tukey s Studentized Range (HSD) Test. Application Equipment: During the growing season, weeds were controlled by hand weeding. All trial treatment applications were applied with a O 2 sprayer equipped with a XR Teejet 8006 VS nozzle at 15 psi in the indicated volume of water. Raw Data See BT114; IR4 Botrytis Data file for raw data Environmental conditions during the experiment: See BT114; IR4 Botrytis Data file for outdoor environmental conditions.