Milk Separation According to Coagulation Properties. G. Katz, G. Leitner, U. Merin, D. Bezman and L. L. Kuzin

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Milk Separation According to Coagulation Properties G. Katz, G. Leitner, U. Merin, D. Bezman and L. L. Kuzin

Question: 1. What is considered "good milk? 2. Are there different "good milks? 1. More complicated than a simple answer 2. For the farmer Animals/gland level For the Dairy industry Bulk Milk

Milk value The value of the milk depends on quality and composition 12 Milk value for Cheese Manufacturing USA Cheese yield (per 100 lb milk) Value of cheese (@$0.55 per lb) 11 10 9 8 7 6 Cheese yield Cheese value 5 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 Percent protein in cheese vat Borg, 1971. Journal of Dairy Science, 54:1134 1136

Milk Milk yield yield and and composition presents during the the lactation status of should represent the needs of the Calf modern High yield Cow yield Lactose Fat Protein

To produce milk, the modern cows require three times more energy then it needs survive. Every deviation from balance energy equilibrium has great impact on milk quality and composition. Diet Genetics Stage of lactation Parity Health Environment Quality milk varies between cows, between milking sessions and during the milking process itself.

qual milk parameters (fat, casein, SCC, bacterial count ) Results in different cheese yield Leitner, G., N. Silanikove, S. Jacobi, L. Weisblit, S. Bernstein and U. Merin. (2007). The influence of milk storage time on the farm and in dairy silos on its quality for cheese production. Int. Dairy J., 18:109 113.

Milk Products butter Churning (high % of fat) fermentation (controlled % of fat) Whole or Low fat milk cheese coagulation yoghurt (high coagulation property) soap (controlled % of proteins)

Cheese Coagulation Cheese formation is the product of casein micelles aggregation to a form of a cage. Hydrophobic inside and hydrophilic outside. This formation traps the solids and looses the water and whey. 1.Proteolysis 2.Aggregation 3.Gelation

The billiard balls model of aggregation Non reactive collision Reactive collision Loose curd formation Full reactive surface Solid curd formation Yield and quality of cheese production depends not only on quantity of caseins but also on the sticking probability of casein collision manifested in protein efficiency

Electronic Microscopy Milk Aggregating Aggregation of milk: Casein matrix(white) holding the fat globules(yellow). Two strep. type bacteria (in light blue) M. Kalab

Two parameters are tested using the Optigraph (Alliance Instruments) to define milk quality for cheese Clotting time (in seconds) Curd firmness (volts) 30 min after addition of coagulating enzyme

Curd firmness (volts) Clotting time (sec) 1 2 3

AfiLab Optical Milk Analyzer On line real time analysis milk of constituents at every stall Fat Protein Lactose Coagulation Property Enables real time routing of milk per its properties

Can milk coagulation properties be used for on line sensing and separation of milk according to the manufacturer need? Vat milk Average milk of all cows SCC, Fat, Protein and Casein Average product

Vat milk Average milk of all cows SCC, Fat, Protein and Casein Average product?? Cheese Maximal product Drinking milk Maximal product

Milk Routing During Milking Milk Properties Change During Milking Cycle Cow 1 Coagulating Milk Milk Cow 2 Coagulating Milk Milk

330 cows Separations: 100%; 5 & 95%; 30 & 70% Target or Target separation Fat (kg/l) Protein (kg/l) Lactose (kg/l) SCC (x1000) 100% 45.4 32.3 49.6 213 95% 45.8 33.0 49.7 215 30% 53.7 34.8 49.8 230 Leitner G., U. Merin, L. Lemberskiy Kuzin, D. Bezman, G. Katz (2012). Real time visual/near infrared analysis of milk clotting parameters for industrial applications. Animal (in press).

132 L 100% 95% 30% 14.1 kg 14.6 kg 17.2 kg L/kg 9.4 9.0 7.7

Case Study: Jacobs, Israel Phase 0 Small Scale Test Jacobs a small dairy Hard and semi hard cheeses Rest of the milk is used for Yogurts A single dedicated farm 120 cows, 18 stalls Test period 9 months in 2012 Producing 4000 Lit daily

How does it Work? Stall 1 Stall 2 Stall 3 Stall 4 Online Analysis Real time Routing Coagulating Milk Milk

MSS Alpha initial results: 8/11/11 Yield 591.42 199.32 790.74 log(scc) 2.50 2.73 2.59 Lactose 4.73 4.69 4.75 Protein Fat 2.90 3.20 3.05 3.68 5.15 4.30 Vat 1 Vat 2 Total

MSS Alpha Preliminary Results : Performance by Cow protein V2 3.04 3.18 3.03 0 0 3.55 3.44 2.89 protein V1 2.73 3.04 2.90 2.83 2.87 0 3.37 2.53 total protein 2.74 3.05 2.98 2.83 2.87 3.55 3.40 2.60 fat V2 4.49 3.71 4.90 0 0 4.98 4.93 5.03 fat V1 3.54 2.69 3.97 5.21 3.84 0 3.61 3.45 total fat 3.57 2.76 4.59 5.21 3.84 4.98 4.32 3.77 V2 yield V1 Yield 0.22 0.44 6.53 0.00 0.00 5.23 4.94 2.63 total yield 8.88 9.10 6.31 6.75 3.28 9.81 11.7911.79 9.65 9.65 0.00 5.23 4.17 9.11 11.8614.49 Cow 100 179 209 213 248 290 302 309

Single cow performance at different cutoffs

MSS pilot results milk processing into Gouda 5.5 5.0 Fat Protein Milk constituents (%) 4.5 4.0 3.5 3.0 Control A 70-76 A 36-50 A 21-24 Milk type Cheese yield (kg/100 L) Cheese Yield efficiency (%) Van Slyke yield (kg/100 L) (42% moisture) Protein efficiency (%) Control 10.47 0 10.53 0.0 A 70 76% 10.87 3.8 10.98 1.4 A 39 50% 11.45 9.3 11.91 4.9 A 21 24% 11.98 14.4 11.11 10.4

Expected Composition of Segregated Milk Coagulation: Maximal Fat: High (4.5 5%) Proteins: High (3.5 3.9%) Casein: High (2.6 2.9%) Lactose: Same SCC: Same Coagulation: Minimal Fat: Low (2 3%) Proteins: Normal (3.1 3.3%) Casein: Normal (2.2 2.4%) Lactose: Same SCC: Same Milk Optimized for Cheeses Coagulating Milk Milk Optimized for Other Products Milk

MSS Milking Parlor System Connections Milking Point 1 AfiMilk MPC Architecture Power Supply & Local Control Milk Meter AfiLAB Receiving Vessel Pump Bulk Tanks Porous Milk Connections Milking Point N AfiMilk MPC Coagulating Milk AfiLAB Milk Meter Wash MSS Management Software

MSS Milk Flow Architecture Milking Parlor Farm 1 Milk Processor / Dairy Recipient Tanks Planning and Management Milk Coagulating Milk Coagulating Milk Milk Central Milk Allocation & Logistics Milking Parlor Farm N Cheese Plant Other Dairy Products Plant Milk Coagulating Milk MSS Management Software

Summary Focus on the milk going into the vat Present Future Management by exceptions performed applied to manage the contents of the milk vat