Anogen --- Manufacturer of Quality Antibody Products

Anogen --- Manufacturer of Quality Antibody Products Cytokine Antibodies ISO 13485: 2016 Quality Management System for Medical Devices

ABOUT US Based in Ontario, Canada, Anogen has been creating leading edge products and offering customizable solutions for cytokine research since 1989. We support our clients worldwide. Our antibodies and assay kits ensure research procedures' accuracy, stability and sensitivity. We support research institutions, universities and CROs. We develop and produce antibodies for research, diagnostic and therapeutic applications to combat various diseases including cancer, inflammatory disorders, neurological disorders, and infectious diseases. Information of our cytokine antibodies and assay kits is available on our website at www.anogen.ca: Monoclonal and Polyclonal Antibodies to Human Cytokines and Growth Factors Matched Antibody Pairs to Human Cytokines and Growth Factors Elisa kits for Human Cytokines and Receptors Elisa kits for Mouse Cytokines and Receptors Multiplex Elisa kits for Human Cytokines 2.

CYTOKINE STORM A cytokine storm is the unconstrained release of cytokines during immune responses, consisting of a positive feedback loop between cytokines and leukocytes. Cytokine storm is potentially fatal, as seen in the extensive cytokine production in infectious and non-infectious diseases including sepsis, avian influenza, H1N1 influenza, severe acute respiratory syndrome (SARS) coronavirus infection, smallpox outbreak, Ebola virus infection, graft versus host disease (GVHD), acute respiratory distress syndrome (ARDS), trauma, and so on Uncontrollable, prolonged cytokine release is also observed in rheumatoid arthritis, psoriasis and other chronic inflammatory diseases. Elevated Cytokine Expression Systemic septic shock: TNF-α, IL-1, IL-6, IL-12, IFN-γ and MIF. SARS: IFN-γ, IL-18, TGF-β, IL-6, IP-10, MCP-1, MIG and IL-8. ARDS: Significantly higher levels of TNF-α, IL-1β, IL-6 and IL-8 in bronchoalveolar lavage. Rheumatoid arthritis: TNF-α, IFN-γ and IL-17 Psoriasis: Cutaneous and systemic overexpression of several pro-inflammatory cytokines, such as IL-2, IL-6, IL- 8, IL-12, IFN-γ and TNF-α. IL-17and IL-23 Cytokine Monoclonal Antibodies Developed and Produced in Anogen bfgf, EGF, Erythropoietin (EPO), TGF-β1 IL-1β, IL-2, IL-6, IL-8, IL-10, IL-16, IL-17A, IL-18 GM-CSF, GCSFR MCP-1(MCAF), MCP-3, MCSF, MIP-1α IFN- γ, TNF-α Anogen s Progress in Anti-Cytokine Storm Therapy Developed AbCream: A topical application of IL-8 monoclonal antibody to treat psoriasis. Developed humanized IL-8 and MCP-1 monoclonal antibody. The humanized IL-8 antibody prevented neutrophil infiltration in acute lung injury (ALI) animal model, indicating a promising future for utilization of humanized IL-8 antibody in the prevention and treatment of ALI in human. 3.

ANOGEN CYTOKINE ANTIBODIES AND ASSAY KITS Antibodies (Hybridomas for the antibodies are developed in Anogen) Product Clone Catalog Number Size A Size B Specificity Number TNF-α CH8810 MO-C40003A 0.5 mg 0.1 mg CH8820 MO-C40003B 0.5 mg 0.1 mg C2H9 MO-C40003D 0.5 mg 0.1 mg B1E4 MO-C40003E 0.5 mg 0.1 mg C2A7 MO-C40003F 0.5 mg 0.1 mg C5G7 MO-C40003G 0.5 mg 0.1 mg C4C7 MO-C40003H 0.5 mg 0.1 mg IFN-γ 315 MO-C40004D 0.5 mg 0.1 mg 165 MO-C40004E 0.5 mg 0.1 mg 23 MO-C40004F 0.5 mg 0.1 mg TGF-β TB21 MO-C40009A 0.5 mg 0.1 mg 2C5 MO-C40009B 0.5 mg 0.1 mg 2C6 MO-C40009D 0.5 mg 0.1 mg 2E6 MO-C40009E 0.5 mg 0.1 mg 8C4 MO-C40009F 0.5 mg 0.1 mg IL-8 I8-S2 MO-C40017A 0.5 mg 0.1 mg I8-60 MO-C40017D 0.5 mg 0.1 mg I8-61 MO-C40017E 0.5 mg 0.1 mg 257 MO-C40017F 0.5 mg 0.1 mg 807 MO-C40017G 0.5 mg 0.1 mg MCP-1/ S8 MO-C40021A 0.5 mg 0.1 mg MCAF S14 MO-C40021B 0.5 mg 0.1 mg S101 MO-C40021D 0.5 mg 0.1 mg 9G10 MO-C40021E 0.5 mg 0.1 mg S382 MO-C40021F 0.5 mg 0.1 mg IL-1β S1H12 MO-C40029A 0.5 mg 0.1 mg S1B7 MO-C40029B 0.5 mg 0.1 mg S3F12 MO-C40029G 0.5 mg 0.1 mg 464 MO-C40029H 0.5 mg 0.1 mg IL-2 5A8 MO-C40032A 0.5 mg 0.1 mg 9F9 MO-C40032B 0.5 mg 0.1 mg 10C4 MO-C40032C 0.5 mg 0.1 mg 8C2 MO-C40032D 0.5 mg 0.1 mg 6F11 MO-C40032E 0.5 mg 0.1 mg 6B1 MO-C40032F 0.5 mg 0.1 mg EGF S-21 MO-C40044A 0.5 mg 0.1 mg S-120 MO-C40044B 0.5 mg 0.1 mg S-134 MO-C40044D 0.5 mg 0.1 mg S-145 MO-C40044E 0.5 mg 0.1 mg S-146 MO-C40044F 0.5 mg 0.1 mg S-147 MO-C40044G 0.5 mg 0.1 mg S-177 MO-C40044H 0.5 mg 0.1 mg G-CSFR S-1252 MO-C40045A 0.5 mg 0.1 mg S-1268 MO-C40045B 0.5 mg 0.1 mg S-1284 MO-C40045D 0.5 mg 0.1 mg S-1374 MO-C40045E 0.5 mg 0.1 mg S-425 MO-C40045F 0.5 mg 0.1 mg S-1390 MO-C40045G 0.5 mg 0.1 mg bfgf F-343 MO-C40046A 0.5 mg 0.1 mg F-474 MO-C40046F 0.5 mg 0.1 mg F-3 MO-C40046H 0.5 mg 0.1 mg F-74 MO-C40046B 0.5 mg 0.1 mg EPO 85 MO-C40047A 0.5 mg 0.1 mg IL-18 2A10 MO-C40094A 0.5 mg 0.1 mg 10H4 MO-C40094B 0.5 mg 0.1 mg Product Clone Catalog Number Size A Size B Specificity Number MIP-1α 116 MO-C40051A 0.5 mg 0.1 mg 199 MO-C40051G 0.5 mg 0.1 mg 548 MO-C40051F 0.5 mg 0.1 mg 125 MO-C40051D 0.5 mg 0.1 mg 400 MO-C40051L 0.5 mg 0.1 mg 103 MO-C40051M 0.5 mg 0.1 mg 376 MO-C40051N 0.5 mg 0.1 mg 426 MO-C40051P 0.5 mg 0.1 mg MCP-3 113 MO-C40088A 0.5 mg 0.1 mg 343 MO-C40088B 0.5 mg 0.1 mg 490 MO-C40088D 0.5 mg 0.1 mg 768 MO-C40088E 0.5 mg 0.1 mg 1057 MO-C40088F 0.5 mg 0.1 mg 542 MO-C40088G 0.5 mg 0.1 mg 1015 MO-C40088H 0.5 mg 0.1 mg IL-16 59 MO-C40089G 0.5 mg 0.1 mg 210 MO-C40089H 0.5 mg 0.1 mg 323 MO-C40089J 0.5 mg 0.1 mg 568 MO-C40089M 0.5 mg 0.1 mg 398 MO-C40089K 0.5 mg 0.1 mg 572 MO-C40089N 0.5 mg 0.1 mg GM-CSF 29 MO-C40090A 0.5 mg 0.1 mg 59 MO-C40090B 0.5 mg 0.1 mg 429 MO-C40090D 0.5 mg 0.1 mg 204 MO-C40090E 0.5 mg 0.1 mg 120 MO-C40090G 0.5 mg 0.1 mg 26 MO-C40090H 0.5 mg 0.1 mg 174 MO-C40090K 0.5 mg 0.1 mg S333 MO-C40090L 0.5 mg 0.1 mg 489 MO-C40090F 0.5 mg 0.1 mg IL-6 2E10 MO-C40091B 0.5 mg 0.1 mg 8E10 MO-C40091C 0.5 mg 0.1 mg 5G8 MO-C40091D 0.5 mg 0.1 mg 1B6 MO-C40091E 0.5 mg 0.1 mg 1E3 MO-C40091F 0.5 mg 0.1 mg 6D7 MO-C40091G 0.5 mg 0.1 mg 9D8 MO-C40091H 0.5 mg 0.1 mg IL-17A 4C1 MO-C40093A 0.5 mg 0.1 mg 2F3 MO-C40093B 0.5 mg 0.1 mg 8H7 MO-C40093C 0.5 mg 0.1 mg 5B4 MO-C40093D 0.5 mg 0.1 mg 1 E2 MO-C40093E 0.5 mg 0.1 mg 3C1 MO-C40093I 0.5 mg 0.1 mg 3H11 MO-C40093K 0.5 mg 0.1 mg 5A9 MO-C40093M 0.5 mg 0.1 mg 6F12 MO-C40093O 0.5 mg 0.1 mg 7F10 MO-C40093S 0.5 mg 0.1 mg M-CSF 116 MO-C40048A 0.5 mg 0.1 mg 692 MO-C40048B 0.5 mg 0.1 mg 21 MO-C40048D 0.5 mg 0.1 mg IL-10 4H2 MO-C40092A 0.5 mg 0.1 mg 6D2 MO-C40092B 0.5 mg 0.1 mg IL-8 Polyclonal AI60001 1.0 mg 4.

ANOGEN CYTOKINE ANTIBODIES AND ASSAY KITS Matched Antibody Pairs (The antibody pairs can be used in sandwich ELISA application) Product Specificity Intended Use Clone Number Catalogue Number Unit Size Tumor Necrosis Factor alpha (TNF-a) Capture CH8820 MO-C40003B 0.5 mg Tumor Necrosis Factor alpha (TNF-a) (**Biotin Labeled) Tracer** B1E4 MO-C40003TB 0.1 Tumor Necrosis Factor alpha (TNF-a) (*HRP Labeled) Tracer** B1E4 MO-C40003T 0.2 Tumor Necrosis Factor alpha (TNF-a) (Unconjugated) Detector B1E4 MO-C40003E 0.5 mg Interleukin-8 (IL-8) Capture I8-S2 MO-C40017A 0.5 mg Interleukin-8 (IL-8) (*HRP Labeled) Tracer* I8-60 MO-C40017T 0.2 Interleukin-8 (IL-8) (**Biotin Labeled) Tracer** I8-60 MO-C40017TB 0.1 Interleukin-8 (IL-8) (Unconjugated) Detector I8-60 MO-C40017D 0.5 mg Monocyte Chemotactic Protein-1 (MCP-1/ MCAF) Capture S14 MO-C40021B 0.5 mg Monocyte Chemotactic Protein-1(MCP-1/ MCAF)(*HRP Labeled) Tracer* S101 MO-C40021T 0.2 Monocyte Chemotactic Protein-1 (MCP-1/ MCAF) (**Biotin Labeled) Tracer** S101 MO-C40021TB 0.1 Monocyte Chemotactic Protein-1 (MCP-1/ MCAF) (Unconjugated) Detector S101 MO-C40021D 0.5 mg Epidermal Growth Factor (EGF) Capture S-134 MO-C40044D 0.5 mg Epidermal Growth Factor (EGF) (*HRP Labeled) Tracer* S-146 MO-C40044T 0.2 Epidermal Growth Factor (EGF) (**Biotin Labeled) Tracer** S-146 MO-C40044TB 0.1 Epidermal Growth Factor (EGF) (Unconjugated) Detector S-146 MO-C40044F 0.5 mg Monocyte Chemotactic Protein-3 (MCP-3) Capture 542 MO-C40088G 0.5 mg Monocyte Chemotactic Protein-3 (MCP-3) (*HRP Labeled) Tracer* 113 MO-C40088T 0.2 Monocyte Chemotactic Protein-3 (MCP-3) (**Biotin Labeled) Tracer** 113 MO-C40088TB 0.1 Monocyte Chemotactic Protein-3 (MCP-3) (Unconjugated) Detector 113 MO-C40088A 0.5 mg Interleukin-16 (IL-16) Capture 398 MO-C40089K 0.5 mg Interleukin-16 (IL-16) (*HRP Labeled) Tracer* 323 MO-C40089T 0.2 Interleukin-16 (IL-16) (Unconjugated) Detector 323 MO-C40089J 0.5 mg Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Capture 59 MO-C40090B 0.5 mg Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) (*HRP Tracer* 429 MO-C40090T 0.2 Labeled) Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) (**Biotin Tracer** 429 MO-C40090TB 0.1 Labeled) Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Detector 429 MO-C40090D 0.5 mg (Unconjugated) Interleukin-18 (IL-18) Capture 2A10 MO-C40094A 0.5 mg Interleukin-18 (IL-18) (**Biotin Labeled) Tracer** 10H4 MO-C40094TB 0.1 Interleukin-18 (IL-18) (Unconjugated) Detector 10H4 MO-C40094B 0.5 mg Multiplex Human Cytokine ELISA Kits Product Specificity Application Catalogue Number For simultaneous quantitive determination of 8 pro-inflammatory cytokine EM10001 Inflammatory Cytokines (Interleukin-1α, Interleukin-1β, Interleukin-6, Interleukin-8, Interferon-γ, Granulocyte Macrophage Colony Stimulating Factor, Monocyte Chemotactic and Activating Factor, And Tumor Necrosis Factor-α) M1/M2/MDSC Cytokines (Granulocyte Macrophage Colony Stimulating Factor, Interferon-γ, Interleukin-4, Interleukin-6, Interleukin-10, Interleukin-12, Monocyte Chemotactic and Activating Factor, and Tumor Necrosis Factor-α) Th1/Th2/Th17 Cytokines (Interferon-γ, Interleukin-2, Interleukin-4, Interleukin-10, Interleukin-13, Interleukin-17, Interleukin-22, and Tumor Necrosis Factor-α) For simultaneous quantitive determination of 8 cytokines related to the differentiation of myeloid derived suppressor cells For simultaneous quantitive determination of 8 cytokines related to the differentiation of T helper cells EM10002 EM10003 5.

ANOGEN CYTOKINE ANTIBODIES AND ASSAY KITS Elisa kits for Human and Mouse Cytokines (Formats of 96 wells and 192 wells are available) Product Specificity Sensitivity Assay Range Catalogue Number Interleukin-8 (IL-8), Hu <10 pg/ 10-1600 pg/ EL10008 Monocyte Chemotactic Activating Factor <4 pg/ (MCP-1/MCAF), Hu 4-1600 pg/ EL10009 Epidermal Growth Factor (EGF), Hu <20 pg/ 20-1000 pg/ EL10010 Monocyte Chemotactic Protein-3 (MCP-3), <30 pg/ Hu 30-1600 pg/ EL10016 Tumor Necrosis Factor alpha (TNF-α), Hu <4 pg/ 4-2000 pg/ EL10019 Granulocyte Macrophage- Colony <2 pg/ Stimulating Factor, Hu 2-500 pg/ EL10020 Interleukin-16 (IL-16), Hu <20 pg/ 20-3200 pg/ EL10021 Interleukin-6 (IL-6), Hu <7 pg/ 7-2000 pg/ EL10023 Interferon-Gamma (IFN-γ), Hu <7.5 pg/ 7.5-1600 pg/ EL10024 Interleukin-2 (IL-2), Hu <6.7 pg/ 6.7-2500 pg/ EL10025 Interleukin-4 (IL-4), Hu <6.6 pg/ 6.6-1000 pg/ EL10026 Interleukin-10 (IL-10), Hu <4 pg/ 4-1000 pg/ EL10027 Interleukin-1 beta (IL-1β), Hu <2 pg/ 2-400 pg/ EL10028 Transforming growth factor beta 1 <9 pg/ (TGF-β1), Hu 31.3-2000 pg/ EL10029 Interleukin-12 (IL-12), Hu <5 pg/ 5-1000 pg/ EL10032 Interleukin-2 Soluble Receptor alpha (IL-2 <10 pg/ sra), Hu 10-2000 pg/ EL10033 Interleukin-6 Soluble Receptor (IL-6 sr), <1.75 pg/ml 1.75-400 pg/ EL10034 Hu Interleukin-5 (IL-5), Hu <7.5 pg/ml 7.5-1000 pg/ EL10035 Interleukin-13 Receptor Alpha (IL-13 <10 pg/ml Ra2), Hu 10-500 pg/ EL10039 Interleukin-1 alpha (IL-1α), Hu <5 pg/ml 5-500 pg/ EL10040 Interleukin-7 (IL-7), Hu <1 pg/ml 1.5-50 pg/ EL10041 Interleukin-9 (IL-9), Hu < 0.343 pg/ 0.78-50 pg/ EL10056 Interleukin-18 (IL-18), Hu < 3.25 pg/ 31.25-2000 EL10060 pg/ Product Specificity Sensitivity Assay Range Catalogue Number Interleukin-17A (IL-17A), Mu <2 pg/ 15.6-1000 pg/ MEC1001 Interferon-Gamma (IFN-γ), Mu <7.5 pg/ 50-3200 pg/ MEC1002 Tumor Necrosis Factor-alpha (TNF-α), Mu <4.5 pg/ 15.6-1000 pg/ MEC1003 Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Mu <17 pg/ 50-3200 pg/ MEC1004 Monocyte Chemoattractant Protein-1 <1.7 pg/ (MCP-1/MCAF), Mu 7.8-500 pg/ MEC1005 Interleukin-2 (IL-2), Mu <2.1 pg/ 7.8-500 pg/ MEC1006 Interleukin-5 (IL-5), Mu <0.8 pg/ 3.125-200 pg/ MEC1007 Interleukin-6 (IL-6), Mu < 5.24 pg/ 12.5-800 pg/ MEC1008 Interleukin-4 (IL-4), Mu < 0.8 pg/ 7.8-500 pg/ MEC1009 Interleukin-1β (IL-1β), Mu < 0.8 pg/ 7.8-500 pg/ MEC1010 Interleukin-1α (IL-1α), Mu < 0.8 pg/ 7.8-500 pg/ MEC1011 Transforming Growth Factor β1 < 9.0 pg/ 32.1-2000 pg/ (TGF-β1), Mu MEC1012 Interleukin-11 (IL-11), Hu < 4 pg/ 4-1600 pg/ EL10042 Interleukin-15 (IL-15), Hu < 10 pg/ 10-2000 pg/ EL10044 Macrophage Colony Stimulating < 15 pg/ 120-4000 pg/ Factor (M-CSF), Hu EL10046 Tumor Necrosis Factor beta (TNF-β), < 11 pg/ 11-8000 pg/ Hu EL10047 Basic Fibroblast Growth Factor < 78 pg/ 78-8000 pg/ (b-fgf), Hu EL10048 Interleukin-17A (IL-17A), Hu < 5 pg/ 5-1000 pg/ EL10053 Interleukin-13 (IL-13), Hu < 1 pg/ 15.6-1000pg/ EL10054 Interleukin-22 (IL-22), Hu < 9.2 pg/ 18.8-600 pg/ EL10055 Interleukin-6 (IL-6), high sensitivity, Hu < 1.56 pg/ 1.56-100 pg/ EL10023hs CITATIONS 1. Burns MJ and Furie MB. Borrelia burgdorferi and interleukin-1 promote the transendothelial migration of monocytes in vitro by different mechanisms. Infect Immun. Oct 1998; 66(10): 4875 4883. 2. Cundell DR et al. The effect of aerial parts of Echinacea on the circulating white cell levels and selected immune functions of the aging male Sprague- Dawley rat. Int Immunopharmacol. 2003 Jul;3(7):1041-8. 3. Crawford S et al. Thrombospondin-1 is a major activator of TGF-β1 in vivo. Cell. 1998 June 26; 98:1159-1170. 4. Elisa A et al. A new monocyte chemotactic protein-1/chemokine CC motif ligand-2 competitor limiting neointima formation and myocardial Ischemia/ reperfusion injury in mice. J Am Coll Cardiol. 2010 Nov 23;56(22):1847-57. 5. Joyner DE et al. Potential for modulation of the Fas apoptotic pathway by epidermal growth factor in sarcomas. Sarcoma. 2011, Article ID 847409 6. Ping YF et al. The anti-cancer compound Nordy inhibits CXCR4-mediated production of IL-8 and VEGF by malignant human glioma cells. J Neurooncol. 2007; 84(1):21-9. 7. Randolph G J and Furie MB. A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the transendothelial migration of monocytes in vitro. J. Immunol. 1995; 155:3610-3618. 8. Samy RP et al. Carboxylate microsphere-induced cellular toxicity in human lung fibroblasts. Exp Biol Med (Maywood). 2012 Jun;237(6):635-43. 9. Sarchielli P et al. Chemokine levels in the jugular venous blood of migraine without aura patients during attacks. Headache. 2004 Nov-Dec;44 (10):961-8. 10. Sarchielli P et al. Proinflammatory cytokines, adhesion molecules, and lymphocyte integrin expression in the internal jugular blood of migraine patients without aura assessed ictally. Headache. 2006 Feb;46(2):200-7. 11. Trieu Y et al. Soluble interleukin-13rα2 decoy receptor inhibits Hodgkin s lymphoma growth in vitro and in vivo. Cancer Res, 2004 64; 3271. 12. Uzawa A et al. Cytokine and chemokine profiles in neuromyelitis optica: significance of interleukin-6. Mult Scler. 2010 Dec;16(12):1443-52. 13. Wang LL et al. Expression of CD39 mrna is altered in the peripheral blood of patients with allergic asthma. Biomed Rep. Jan 2014; 2(1): 75 78. 14. Wen MC et al. Efficacy and tolerability of antiasthma herbal medicine intervention in adult patients with moderate-severe allergic asthma. J Allergy Clin Immunol. 2005 Sep;116(3):517-24. 15. Yao XH et al. Production of angiogenic factors by human glioblastoma cells following activation of the G-protein coupled formyl peptide receptor FPR. J Neurooncol. 2008; 86(1):47-53. 6.

WHY ANOGEN ESTABLISHED BY SCIENTISTS in 1989 ISO 13485: 2016 CERTIFIED quality management system for medical devices All products are PRODUCED IN CANADA WORLDWIDE PRESENCE with distributors in over 20 countries IN-HOUSE HYBRIDOMA BANKS, ensuring continuous supply of antibodies COMPREHENSIVE DATA COMPETITIVE PRICING CONTINUOUSLY EXPANDING cytokine product lines Order and ship in the same day for NEXT DAY DELIVERY FREE ANTIBODY SAMPLES are available at www.anogen.ca Inquire about SPECIAL RATES at sales@anogen.ca Q: How would Anogen control product quality? A: Anogen cytokine antibodies are manufactured under a rigorous ISO13485: 2016 quality management system for medical devices. All aspects of manufacturing, testing, labelling, and packaging are stringently controlled, validated and monitored. Anogen provides Certificates of Analysis and MSDS for all product lines. Q: How are Anogen antibodies shipped? A: Most of the products are lyophilized, making them stable at a wide range of temperatures. The package is packed with blue ice packs into a shipping box and shipped to your country. We look forward to collaborating with trustworthy biotech companies at all times! 7.

Anogen Quality Policy Statement Anogen is committed to supplying our customers with the highestquality products and services to achieve customer satisfaction, as well as ensuring compliance with the requirements of ISO 13485: 2016 Quality Management System for Medical Devices. 2355 Derry Road East, Unit 23 Mississauga, Ontario L5S 1V6 Ph: 1-877-755-8324 Fax: 1-905-677-0023 info@anogen.ca anogen.ca