Purity committee. Jane Taylor. ISTA Purity Committee Report 2014

Purity committee Jane Taylor ISTA Purity Committee Report 2014

Purity committee members 1 CHAIR: Jane Taylor United Kingdom 2 VICE-CHAIR: Gerarda de Boer Netherlands 3 Myriam Alvisi Brazil 4 Pernilla Andersson Sweden 5 Karima Boudehri-Giresse France 6 Maria Duter New Zealand 7 Adriel Garay USA 8 Charan Jeet Mehta India 9 Andrea Jonitz Germany 10 Augusto Martinelli Argentina 11 Deborah Meyer USA 12 Kepha Oganda Kenya 13 Dot Vittrup Pedersen Denmark 14 Zita Ripka Hungary 15 Ruojing Wang Canada

Topics Rules proposals Universal List Blowers, Master Calibration Samples Tropical Seed Handbook Purity Workshop, Palmerston North, New Zealand, 2013 X-ray imaging to determine the content of pelleted seeds

Rules proposals 2014 C.3.1. Clarification of rounding procedure in the purity test C.3.2. Clarification of the need to retain the component parts found during the purity analysis C.4.1. Clarification of how to report when seed cannot be identified with certainty to species level C.4.2. Clarification of the need to retain any seeds found during the other seed determination analysis C.10.1. Clarification that 10.5.3 applies to all chaffy seeds and the procedure for weighing replicates

Rules proposals 2014 - C.3.1. Clarification of rounding procedure in the purity test The proposal is made to clarify and standardize the rounding procedure for purity components where the second decimal place figure is 0.05% or more, in that the component(s) is rounded up to one decimal place. New wording added 3.6.1.3 Rounding procedure Fraction percentages must be rounded to one decimal place. After rounding, add together the percentages of all fractions.

Rules proposals 2014 - C.3.2. Clarification of the need to retain the component parts found during the purity analysis New wording added 3.6.1.3 Rounding procedure After weighing, the components found must be retained and stored for reference until sample disposal (see 2.5.3 and 2.5.4.7). Other seed Inert matter Pure seed

Rules proposals 2014 - C.4.1. Clarification of how to report when seed cannot be identified with certainty to species level. Addition to 4.7 of the same wording as in 3.7 New wording added...where it is impossible to determine with certainty on the basis of seed characteristics, the genus name only is reported (e.g. Malus sp.)...

Rules proposals 2014 - C.4.2. Clarification of the need to retain any seeds found during the other seed determination analysis New wording added Seeds of the species found must be retained and stored for reference until sample disposal (see 2.5.3 and 2.5.4.7).

Rules proposals 2014 C.10.1 Clarification that 10.5.3 applies to all chaffy seeds and the procedure for weighing replicates 1. The coefficient of 6.0 should apply to all chaffy seeds (see 3.6.6) and not just chaffy grass seed 2. The correct number of decimal places to be used when weighing either the whole purity working sample weight or the weight of the individual replicates. New wording and new Table added to Chapter 10 The working sample or replicates to be weighed to the minimum number of decimal places as indicated in Table 10A.

ISTA Universal List - Digital images Report of the WG - Zita Ripka Progress made Group 1 Ruojing Wang 32 species Complete (images and seed descriptions sent to ISTA Secretariat) Group 2 Corinne Sahuguede 32 species Images ready, text is being checked Group 3 Debbie Meyer 34 species 30/34 completed (images and seed descriptions being verified) Group 4 Zita Ripka 32 species Images ready, text is being checked

ISTA Universal List examples of complete pages Triticum aestivum & Bidens pilosa

ISTA Universal List Plans for 2014 1. Finalise seed descriptions 2. Download files to ISTA Secretariat 3. Make the tool work on the website / Nadine Ettel Special thanks to the working group members Zita Ripka, Debbie Meyer, Corinne Sahuguede, Ruojing Wang and Nadine Ettel.

Blower Calibration Samples Report of the WG - Gerarda de Boer Completed: Comparison ISTA and AOSA calibration samples - Results: Poa sample comparablity is very good, but Dactylis not in agreement Rules proposal on Determination of the Equivalent Air Velocity (EAV) using an anemometer - Result: The method is now in the current ISTA rules Date - Presenter Name

Feedback on the new method using the anemometer Since 01-01-2014 in the ISTA rules We would like to know about your experience with the method Buying an anemometer Calibration of the anemometer Daily use?

Check your own calibration sample Purity committee gives guidelines on blower calibrations (doc. from 27-05-2009) Composition Preparing half-calibration samples Storage Checking of samples Uniform blowing procedure Trouble shooting The document is being reviewed

Future issues Comparing ISTA and AOSA Dactylis calibration samples - differences in protocol - validation study Introduce master calibration sample? Re-calibration of ISTA calibration samples Update the guidelines for blower calibrations on ISTA web-site Other issues you may raise?

Members of the blowing working group Pernille Hvolbøl Jensen (Denmark) Dot Vittrup Pedersen (Denmark) Karima Boudehri (France) Adriel Garay (USA) Ruojing Wang (Canada) Deborah Meyer (USA) Jane Taylor (UK) Many thanks to you all

Handbook on TROPICAL SPECIES Report of the WG Myriam Alvisi SEED IDENTIFICATION - COLLECTION OF PHOTOGRAPHS AND SUMMARIZED DESCRIPTIONS OF 7 SPECIES OF BRACHIARIA AND 38 SPECIES IDENTIFIED IN BRAZIL AS UNDESIRABLE SEEDS (WEEDS) MORE FREQUENT IN SAMPLES OF THE BRACHIARIA GENUS Coordination: Myriam Alvisi Execution: Official Seed Testing LAB staff (LASO-MG, Brazil): - Fernanda Cássia de Oliveira - Maria Izabel Furst Gonçalves Photographs: Ronaldo Linares Sanches (LANAGRO-MG, Brazil) Revision of descriptions: Dr Doris Groth

DR DORIS GROTH WITH THE LASO-MG S STAFF

FAMILY GENERA Number of species COMMENTS ASTERACEAE Acanthospermum 1 undesirable COMMELIACEAE Commelina 2 undesirable CONVULVULACEAE Ipomoea 10 undesirable EUPHORBIACEAE Croton 1 undesirable FABACEAE/ CAESALPINOIDEAE Senna 2 undesirable MALVACEAE Sida 9 undesirable 7 cultivated/ Brachiaria undesirable Cenchrus 1 undesirable POACEAE Digitaria 3 undesirable Panicum 1 cultivated/ undesirable Paspalum. 5 undesirable Pennisetum 1 undesirable RUBIACEAE Diodia 1 undesirable Spermacoce 1 undesirable

sheets Family Scientific name Synonyms Common name Dispersion unit Size Summarized Description Importance Origin and distribuition

POACEAE (= GRAMINEAE) Brachiaria humidicola (Rendle) Schweick. (= Urochloa humidicola (Rendle) Morrone) Synonyms: Panicum humidicola Rendle Common names: Braquiária, braquiarinha, braquiária-passopasso, capim- agulha, espetudinha, grama-do- Pará, kikuiu-da-amazônia. Dispersal units: spikelets and rarely fertile florets. Dimensions: (length x width x thickness) Spikelet: 4.0-5.5 x 1.8(-1.9)-2.4 x 0.9-1.2mm Fertile floret: 3.0-(3.4-)4.4(-4.8) x 1.6-2.0(-2.1) x 0.7-1.0mm Caryopsis: 2.4-(2.5-)3.0 x 1.3-1.8 x 0.5-.,8mm Description: spikelet, ellipsoid with an acuminate apex, flat-based or peduncled, straw colored to light-yellow, glabrous or with sparse pilosity and anastomotic transversal ribs (united in the apex); superior glume very reticulated, with apical and marginal pilosity; inferior glume does not embrace the spikelet base (just touches) and has the same length as the spikelet on the ventral side; fertile floret, ellipsoid, straw to light-brown, glabrous, lemma and fertile palea lightly striated; caryopsis, obovate with cream-coloured. Picture 1: On the left, spikelet, fertile floret and caryopsis in the dorsal side. On the right, spikelet, fertile floret and caryopsis in the ventral side. a' a b e Importance: resilient grass, commonly used in pasture formation, particularly in humid lands. It may also become an undesirable crop weed. Origin and distribution: native to the eastern and the south-eastern parts of Africa. Naturalized to South America, including Brazil. Cultivated in Brazil, Colombia and Trinidad and Tobago. a '' f d b c Source: ALCÂNTARA (2013); BRASIL (2009); GRIN (2014); GROTH (2014); ISTA (2013); KISSMANN (1997). Prepared by: Fernanda Cássia de Oliveira and Maria Izabel Furst Gonçalves (LASO/LANAGRO-MG/MAPA) Pictures: Ronaldo Sanches. LANAGRO/MG/MAPA - Brazil Picture 2: a) inferior glume, a - superior glume, a' - sterile lemmal; b) spikelet, dorsal view; c) spikelet, espigueta, ventral view; d) fertile floret, dorsal view (fertile lemma); e) fertile floret, ventral view (fertile palea); f) caryopsis, dorsal view. Date - Presenter Name

SEED IDENTIFICATION BY Dr DORIS GROTH INDIVIDUAL DRAWING OF ALL THE PHOTOGRAPHED SPECIES GROUP DRAWING OF SOME GENERA INDICATION OF MORPHOLOGICAL STRUCTURES BOTANICAL DESCRIPTIONS

FUTURE ACTIONS -Translation into English sheets -Last revision -Make available the Handbook s first version in English for appreciation, discussion, criticisms and suggestions.

ISTA WORKSHOP 30 Sept 02 Oct 2013 ISTA SEED PURITY & IDENTIFICATION WORKSHOP AsureQuality Palmerston North Seed Laboratory, New Zealand 20 participants (NZ, Australia, China) Many thanks to Craig McGill, Sue Cousins and David Geary and the laboratory staff for all their help Also grateful thanks to Deborah Meyer for permission to use her presentations and Gerarda de Boer for her assistance in checking detail in presentations.

WORKSHOP 30 Sept 02 Oct 2013 PURITY TOPICS An introduction to ISTA History & Aims The principles of the ISTA Analytical Purity Test Botanical terminology for purity testing Practical application of ISTA rules for Purity Pure Seed Definitions (PSD) Other Seed Determination Seed retrieval & identification Results, tolerances, calculations & reporting Uniform blowing procedure Questions to the purity committee

Purity and Seed Id Cuscuta spp. evaluation Triticum Hordeum Medicago Trifolium Brassica Orobanche Lolium Poa Festuca

New Techniques X-ray imaging to determine the content of pelleted seeds Presentation by Karima Boudehri-Giresse

Purity analysis on coated seeds Following ISTA rules : 100 seeds with all coating removed (washing) Constraints : Exposure of analyst to chemical treatments Destructive method Specific procedure for disposal of waste Time consuming After washing if <100 seeds : empty pellet or lost seed? => Using X-Ray for purity on coated seeds

Impact of different coating on image quality 2D Xray Tomography (visualization by slices)

Empty pellets in coated samples 2D Xray Empty pellets Tomography (visualization by slices)

Identification of the species 2D Xray Tomography (visualization by slices)

Future possibility Availability of an alternative method for the laboratories with X-Ray equipment Using X-Ray for purity analysis on coated seeds In-house validated method approved by the Purity committee Able to identify empty pellets Easy to analyze more than 100 seeds Extend to OSD Possibility of developing an automated detection

ISTA Purity Committee Thank you for your attention. Grateful thanks to the purity committee members for their contributions to the work of the committee and this presentation. Date - Presenter Name