Durian. Pineapple 9/22/2015 JACKFRUIT. Processing and Handling of Tropical Fresh-cut Fruits. Latifah Mohd Nor & Ab Aziz Ibrahim

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Processing and Handling of Tropical Fresh-cut Fruits Latifah Mohd Nor & Ab Aziz Ibrahim E-ml: lmn14106@gmail.com The needs for tropical fruits to be marketed in fresh-cut/ready to eat is greater as compared to the temperate fruits 1. Huge size 2. High price particularly early in the season 3. Difficulties in cutting 4. Risk in obtaining poor quality pulp 5. Weight reduction for transportation and avoiding plant quarantine 6. Overcome waste disposable problems at the importer ends. MALAYSIAN AGRICULTURAL RESEARCH AND DEVELOPMENT INSTITUTE JACKFRUIT Unique for its size with outer blunt spinel skin. Depending on fruit size, there may be as many as 50 to 100 edible bulbs embedded in a single fruit interspersed between thin bands of fibersepidermal layers Durian The fruit is distinctive for its large size, unique odor, and formidable thorn-covered husk. Flesh - creamy-yellow to saffron color depending on the species. The pulp soften, turns slimy and deteriorates rapidly, resulting in off-flavour and astringent odour being produced Pineapple Pineapple is one of the popular fruits served in fresh-cut form world wide. At ambient temperature and without protective treatments, cut pineapple turns slimy and deteriorates rapidly, resulting in the development of off flavours and odours within a day. Fresh cut products are prepared and marketed without an effective microbial killing control steps. New unforeseen food safety issues have emerged as major concerns in the fresh-cut pocessing industry of perishable produce leads to the requirements of special handling during processing, transportation and storage at the market outlets. 1

Accelerates rate A of respiration *Causing mechanical damage *Disrupts cell membranes *Exposed fruit to microbial contamination *Mechanical shock to tissue *Alter gas diffusion The protective layers has been removed. *Creates the opportunity for the contamination and spoilage by pathogenic organism. Presentation: Approaches for processing and handing of fresh-cut jackfruit, durian and pineapple for local and export markets. **Issues involved in maintaining both quality and safety during market distribution. HANDLING OPERATIONS Basic research Intensive research upscaling Simulation studies and actual export trials by different modes of transportation Fruit maturity Selection of the optimum fruit maturity for fresh-cut processing is essential: To provide the best combination of eating quality and postharvest life. Maturity at cutting will determine the optimum temperature for storage. However, maturity requirement varies depending on fruit types. Depending on the variety, jackfruit mature about 180 to 270 days after flowering. Matured fruit will show the following sign: -Fruit colour changes from green to pale green. -A dull, hollow sound is produced when the fruit is tapped by the finger. -Fruit spinel become well developed and wide spaced. -The last leaf of the peduncle yellows. -An aromatic odour develops. Fresh-cut processing to matured fruits Matured fruit pulp durian remain hard after cutting. Thus fresh-cut processing only been conducted to the ripen fruits. 2

For good eating quality, Josapine pineapple should achieve index 3-4 (20% of the eyes are yellowish orange) Josapine MD2 Ensure of even fruit ripening prior to cutting Facilitate isolating the fruitlets & less condensation to the stored product Normal operation of cutting VIDEO Processing & Handling Operations VIDEO The first device faciliting the cutting process while the second tool is used to assist in exposing the edible bulb upward, the edible bulbs loosen, which helps the operator to speed up the isolating process. Isolating edible bulb: manual vs hand tool The devices are very effective as it reduces mechanical injuries to the edible bulbs. Furthermore, it also speeds up the overall cutting and isolating the edible bulbs during the minimal processing operations. 3

Milano 2015 Packing, labelling & bar coding a b Fig 2: Effect of pre frozen treatment and packing system to the fresh-cut durian stored at different retail outlets (Hong Kong) & control samples stored at MARDI (Malaysia) - 2014 4

Sydney 2013 MALAYSIAN AGRICULTURAL RESEARCH AND DEVELOPMENT INSTITUTE [Horticulture Research Centre Skin peeler Fruit slicer Pre-treatments reduce microbial spoilage, excessive tissue softening and browning are needed for quality improvement of fresh-cut pineapple. The cut pieces need to be immersed in the pre-treatment solution at the final stage of the handling operations. *Flesh firmness of the cut pineapple can be maintained by using calcium salts (Latifah et al., 1999) *Oxidative browning can be overcome by using ascorbic acid (Latifah et al., 2000). *The used of citric acid appeared to be beneficial for lowering the microbial growth during storage of fresh-cut pineapple at 10 C (Latifah et al., 2011). *The use of citric acid had also reduced the activity of polyphenol oxidase as observed during storage at 10 C (Nur Azlin et al., 2011). 5

Fig.3: Changes in the VRBA (Voilet Red Bile Agar ) of the freshcut pineapple treated with different concentration of citric acid during storage at 10 and 2 C. Drip Drying Modified atmosphere packaging (MAP) by using seal on (a) or shrink wrapping (b) packing system suitable to maintain the freshness of fresh-cut fruit (2-3 weeks at 2 C, one week at 10 ºC and two days at 25 ºC). Seal packing system Quality & Safety a b 1. Physical changes (wt loss, colour) 2. Physiological (respiration & enzymes activity) 3. Microbial growth (mould & yeast, coliform) 6

Evaluation of colour -L, a & b Measuring the respiration rate Must be low & within acceptable limit based on the Malaysian Food Regulation Quality evaluation of fresh cut durian Hong Kong 2014 A : 200g Prefrozen 5 weeks B : 200g Prefrozen 5 weeks Fig 4: Total plate count of fresh-cut durian packed using seal pack (200g) and shrink wrap (400g), at different pre frozen treatment (1,5 & 3 wks), stored at different retail outlets in Hong Kong. Control samples stored at MARDI (Malaysia) C : 200g Prefozen 1 week D : 400g prefozen 5 weeks E : 400g prefrozen 3 weeks 1: AEON TuenMuen 2: AEON Tsuen Wan 3: AEON Kornhill 4.MARDI (Control) Indicator 2.5 very slight growth 12 slight growth 40 moderate growth 100 heavy growth 250 very heavy growth Bruished - packaging too tight/rough handling Wilting - excessive drying/water loss Mushiness - excessive tissue softening Development of off colourenzymatic browning Undesirable odour/fermented aromaaccumulation of ethanol 7

Export trials before technology transfer for commercialization To evaluate effectiveness of handling operations at all stages: - farm, product preparation - product distribution - product quality at the importer ends Air shipments: *Netherlands (2006)-jackfruit & pineapple *Dubai (2010)-jackfruit *Perth (2011) pineapple *Sydney (2013)-durian * Hong Kong (2014) -durian Sea shipment: *Hong Kong (2008)-durian Cold truck road transportation : Singapore (2009)- pineapple, jackfruit & durian Present export market of Malaysian fruits are to countries which do not impose stringent SPS measures Importing countries include: Hong Kong, Singapore, Brunei, Indonesia, Middle East (Saudi Arabia, UAE), EU Countries Many developed countries impose stringent phytosanitary requirements for import of fresh fruits (e.g Australia, Japan, USA, New Zealand, China, Taiwan ) SPS measures are imposed against quarantine pests Procedures (rule making process for market access approval are lengthty eq: USA: 3 4 years, Australia: 3-5 years Most of tropical fruits are subjected to infestation by fruit flies. Fruit Type Quarantine Pest Market Access Papaya Fruit fly China,US, NZ,Korea Pineapple Mealy bug US, China Rambutan Mealy bug, Fruit fly US, Korea, Japan, Jackfruit Mealybug,fruit fly Australia, US, China,Taiwan Starfruit Fruit fly US, EU,Canada, Korea, China, Quarantine treatment Fruit Type (Whole) Importing country Fumigation Pineapple Australia, China,jackfruit Vapour heat Papaya, mango Australia, Japan Hot water papaya China Quick freezing Durian Australia, China Cold treatment carambola China Irradiation Papaya, mango, rambutan, pineapple USA 8

Fumigation On going R&D activities Evaluate effective quarantine treatments to the whole fruits Problems encountered -affect physical, chemical, physiological attributes and overall acceptance (taste & texture). Example jackfruit- blackening of the skin, uneven ripening, reduction in the fruit sweetness Fresh- cut processing allows each fruits to be carefully examined right to the fruit centre. Thus fresh-cut processing is been accepted as tool for a quarantine treatment to enhance market access. Australia China New Zealand Taiwan Australia & New Zealand (ok) Australia (ok) Fresh-cut durian - Sydney 2013 Fresh-cut durian Hong Kong - 2014 FRESH-CUT FRUITS - Facing various challenges due to the nature of the product itself. PRODUCT PROMOTIONS 9

TV programmes LOCAL EVENTS/ ROAD SHOWS ASIA FRUIT LOGISTICA 2014- HONG KONG INSTORE PROMOTION- SWEDEN (APRIL 2015) Milano 2015 (Milan, Italy) Program : presentation/video/poster/leaflets Seed 4- Malaysian Pavilion (7-13/7/2015) Demo- how to cut the jackfruit and isolate the fruitlets Product testing 10

Consumer demand for convenience and added value means that fresh-cut products will play a significant role in the fruit industry in the future. A great challenge to deliver the prime quality products with extended shelf life to the consumers especially for export markets. In order to achieve highest quality and longest shelf life, suitable PROCESSING & PACKAGING technology should be employed. As for today, technology on fresh-cut processing developed by MARDI from research and development activities became a breakthrough innovation, first for the world markets as proven through the series of successfull export trials and commercialization activities thereafter. However, the issues of safety for the fresh-cut must be emphasis seriously since even a minor mistake could lead to disaster and the demise of an emerging industry. Acknowledgements The authors would like to acknowledge Ministry of Agriculture and Agro based Industries (MOA) and Malaysian Agricultural Research and Development Institute (MARDI) for the financial supports. Also to all Postharvest Staffs involved direct or indirectly during the R&D activities. Sincere thanks also to all our colloborators involve in the project local and abroad during the export trials 11