Agri Analysis Inc. Instructions for Taking Samples for Plant Analysis

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Agri Analysis Inc. Instructions for Taking Samples for Plant Analysis Read these instructions carefully Taking a Sample Use the instructions in this publication to obtain s which accurately reflect nutritional status of the crop submitted for analysis. Results of the analysis will determine whether problems in responses are due to a nutritional problem. Plant analysis can also be used to monitor and maintain appropriate patterns. Completing the Plant Analysis Request Form For every leaf, petiole or fruit sent to the Laboratory for analysis there must be an accompanied request form. The request form is designed to provide a direct communication between the laboratory and the crop specialist who will interpret the results. Be sure to complete this form. Please provide sufficient information for invoicing purposes and always list crop details- stage and plant part d. What to Sample Response of plants to nutritional conditions differ for each variety, stage of development and growing area. As near as possible select plant parts representative of the particular variety and growing area. When sampling mixed grasses and other mixed forages, separate species into different s. What not to Sample Diseased or dead plant material Plant tissue damaged by insects & mechanical Equipment Plant tissue which has been stressed by excess cold, heat or moisture Seeds should not be d- they do not reflect the nutritional status of the whole plant Stage of Asparagus Asparagus officinalis Mid-summer Late-summer Beets Beta vulgaris 4-6 Weeks 8-10 Weeks Broccoli Brassica oleracea var. italica Heading Top cm of fronds Top cm of fronds Common Cabbage Brassica oleracea var. capitata to 10 10 2-6 weeks old Whole tops 2-3 months old Wrapper Head ¾ Mature Wrapper Midribs of wrapper Chinese or Celery Cabbage Brassica rapa var. pekinensis 280 Newport Rd* PO Box 483* Leola PA 17540* 717-656-9326 www.agrianalysis.com

Stage of Growth Carrots Daucus carota ssp. sativus Middle of growing season of new to Oldest Cauliflower Brassica oleracea var. botrytis At heading of new Stage of Endive or Escarole Cichorium endiva to 8 Leaf stage Oldest 10 Kale Brassica oleracea var. acephala Middle of growing season of new Lettuce Lactuca sativa var. capitata Boston/Butterhead Lettuce Celery Apium graveolens var. dulce 6 week old plants, non-flowering of most recent fully developed of new Collards Brassica oleracea var. acephala 8 leaf stage Oldest Fully expanded Lactuca sativa var. capitata Greenhouse Lettuce (Boston/Butterhead) prior to heading Lactuca sativa var. capitata Head/Iceburg Lettuce of new Middle of growing season Cucumber Cucumis sativus Bud to small fruit 5th leaf blade Wrapper Heading Leaves of mature head Lactuca sativa var. longifolia Cos/Romaine Lettuce Wrapper Small fruit to harvest 5th leaf blade Onion Allium cepa Eggplant Solanum melongene 1/3 to 1/2 maturity Whole tops (green portion only) Bud to small fruit 1/2 grown to maturity Whole top(green portion only)

Stage of Plant part to Peas (Garden or English Peas) Pisum sativa ssp. Sativum First bloom Peppers(Bell,Sweet or Green) Capsicum annuum First bloom to fruit fill, young plants Producing abundant fruit Potatoes Solanum tuberosum leaflets to Stage of Pumpkin Cucurbita pepo 5 Weeks from seeding 8 Weeks form Seeding Middle of Growing Season of new and petioles of new and petioles of new Growth to 30 cm tall Tubers ½ grown First Blossom Maturity and petioles and petioles

Stage of Spinach Spinacea oleracea /petio les to Stage of Radish Rhapanus sativas to -30 days old Middle of growing season 30 Rhubarb Rheum rhabarbarum, non-flowering Squash Cucurbita pepo var. melopepo **** Production Greenhouse Blades of fully expanded Bean Snap,Green,String,Kidney or French Phaseolus vulgaris Blades of most recent developed Uppermost recent fullydeveloped trifoliate 10

Stage of Plant part to to Stage of Plant part to to Sweet Corn Zea mays ssp. Mays Tomato Trellis 10- Inches tall 5-6 weeks old, -30 Inches tall 7-8 weeks, tassel start Full tassel to silk start End of silk Sweet Potato Ipomoea batatas, 5th, 5th, 5th, 5th, 5th 8 8 8 8 8 Mid-bloom, 1st Mid-bloom, 2nd Mid-bloom, 3rd Mid-bloom, 4th 1st half of growing season fully developed Tomato Lycopersicon lycopericum Field Grown Mid-bloom Mid- Production Adjacent to or just below Leaves of New Growth 30 Mid-bloom, 5th Mid-bloom, 6th Zucchini Cucurbita pepo var. melopepo Greenhouse Grown Non-Fruiting of new, nonfruiting Mature of new s Fruiting of new

Stage of Alfalfa or Lucerne Medicago sativa Prior to flowering Barley Hordeum vulgare Emergence of Head from Boot Corn Zea mays Field Crops tops-six inches of new /petiole s to Whole Tops Stage of Sorghum Sorghum bicolor Seedling <30 cm tall (23-39 days old) 37-56 days old Blooming stage, visible heads Field Crops to Whole tops 3rd leaf below head Less than '' Whole tops Prior to Tasseling Initial Silk To Grain Fill Grain Filling Stage Leaves below the whorl Base of Ear Leaves Leaves (5 th Leaf Form Tip) Maturity Base of Ear Irrigated Prior to Tassel Grain, dough condition Soybeans Glycine max Early Growth Early Flowering Prior to pod set 3rd leaf below head Most Recently Mature Trifoliate Most Recently Mature Trifoliate Cotton Gossypium hirsutum Pod Filling First squares to initial bloom Full bloom Oats Avena sativa Vegetative stems Vegetative stems Prior to Heading Whole tops Tobacco Nicotiana tabacum 30-45 days after planting 45-60 days after planting Rye Secale cereale 60-80 days after planting Panicle initiation Whole tops Timothy Phleum pratense Early anthesis Whole tops 80-100 days after planting Bloom stage

Stage of Wheat Triticum aestivum Field Crops to Stage of Orchard Grass Dactylis glomerata Field Crops to Stage 1, one shoot Stage 2, tillering begins Stage 3, tillers fomed Stage 4, leaf sheaths lengthen Stage 5, leaf sheaths strongly erected Whole tops Whole tops Whole tops Whole tops Whole tops 5 Weeks after Cutting or Spring Green Up Red Clover Trifolium pratense Whole Tops Prior to flowering Whole Tops White Clover Trifolium repens f. lodigense Prior to flowering Stage 6, first node of stem visible Stage 7, Second node of stem visible Stage 8, last leaf just visible Whole tops Whole tops Whole tops Stage 9, ligule of last leaf just visible Stage 10, in "boot" Stage 10, Flag only Whole tops Whole tops Whole tops

Stage of Apple Leaves Malus domestica Fruits, Berries, Grapes New Blueberry (Rabbit Eye, Highbush, General Blueberry) Vaccinium ashei, corymbosum Cantelope/Muskmelon/Persian Melon Cucumis melo Cantaloupensis Flower start to small fruit 5th leaf, unfurled to 75 Stage of Fruits, Berries, Grapes Peaches or Nectarines prunus persica Pear Pyrus communis Plum Prunus domestica cultivar new new to Small fruit to harvest 5th leaf, unfurled new Cherry Tree (sweet or sour) Prunus avium/cerasus Raspberries Rubus idaeus Cranberry Vaccinium macrocarpon 2-3 wks postharvest of mid-section of non fruiting canes 75

Stage of Strawberries Fragaria xananassa Transplant At Flower Initial Harvest End of Season Watermelon Citrullus lanatus Fruits, Berries, Grapes and fully expanded and and to Stage of Grape Vitis labrusca Full bloom Vitis rotundifolia Vitis vinifera Early summer Full Bloom Fruits, Berries, Grapes basal flower s of new to American Table Grapes Muscadine 75 Wine or European Table Grape Whole bunch basal flower s Flower start to small fruit, small fruit stage Small fruit to harvest, 5th, 5th

Woody Ornamentals and Flowers Stage of Celosia Cockscomb/Crested Celosia argentea (36 cell packs) Daylilies Hemerocallis fulva Delphinium Delphinium x Pacific hybrids Geranium Pelargoium x hortorum to Oenothera (evening primrose) Oenothera speciosa Oriental Arborvitea Platycladus orientalis Petunia Petunia x hybrida (36 Cell Packs) 2-3'' terminal cuttings Japanese Pieris or Andromeda Pieris japonica 2-3'' terminal cuttings 40 40 Rhododendron Rhododendron catawbiense Gladiolus Gladiolus callianthus American or Eastern Arborvitae Thuja occidentalis Hibiscus Hibiscus rosa-sinensis 2-3'' terminal cuttings Viburnum Viburnum cassinoides Kalmia (Mountain Laurel) Kalmia latifolia Zinnia Zinnia elegans (36 Cell Packs) 40

Stage of Douglas Fir Pseudotsuga menziesii Landscape Trees to Ground Covers and Grasses Stage of Kentucky Bluegrass Poa pratensis to Late summer to fall 2-3'' terminal cuttings Clippings from new 2 cups Eastern White Pine Pinus Strobus Terminal cuttings (needles) Red Sunset Maple Acer rubrum Sugar Maple Acer saccharum Tulip Poplar Liriodendron tulipfera