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Florida Cooperative Agricultural Pest Survey Program Quarterly Report No. 2-2010 April 1 to June 30, 2010 Florida Department of Agriculture and Consumer Services Division of Plant Industry 1911 SW 34 th Street PO Box 147100 Gainesville, FL 32608 352.372.3505 Table of Contents: Table of Contents:...2 NAPIS Data Base 3 FDACS-DPI-CAPS and USDA-PPQ-CAPS Personnel...14 Maps:. 15 Map 1: Red Bay Ambrosia Beetle and Laurel Wilt Survey in Miami-Dade County....15 Map 2: Cotton Seed Bug Survey in Monroe County 16 Figures:. 17 Fig. 1: Cotton Seed Bug Survey in Monroe County....17 Fig. 2: CAPS downloadable calendar, May 2010..18 Fig. 3: EDRR Survey in the Alachua County area....19 2

Introduction The Florida Department of Agriculture and Consumer Services-Division of Plant Industry (FDACS- DPI) Cooperative Agriculture Pest Survey (CAPS) program staff was engaged in many program activities including data entry, survey, public outreach, training and program development. Information on each program area is detailed in this report. NAPIS Data Base Table 1a. Pest records entered into the NAPIS database, 2nd Quarter 2010. U.S. Records Pest Scientific Name Tospovirus Groundnut Ringspot Virus (GRSV) Pest Common Name Host Scientific Name Host Common Name County groundnut ringspot virus GRSV Lycopersicon esculentum tomato Miami-Dade Guignardia citricarpa citrus black spot Citrus sinensis (L.) Osbeck sweet orange Collier Paracoccus herreni Williams & Granara de Willink a mealybug Hibiscus rosa-sinensis L. Chinese Hibiscus Manatee Septoria mikania-micranthae Passionfruit mottle virus (PaMV) Septoria leaf spot of Mikania Mikania micrantha Kunth bittervine, mile-aminute Miami-Dade passionfruit mottle virus (PaMV) Passiflora sp. a passionflower Broward Xylosandrus amputatus Blandford an ambrosia beetle N/A N/A Orange Total = 6 State Records Pest Scientific Name Pest Common Name Host Scientific Name Host Common Name County Oxycarenus hyalinipennis (Costa) cotton seed bug Gossypium sp. wild cotton Monroe Hylocurus rudis (LeConte) an ambrosia beetle N/A N/A Hamilton Siphoninus phillyreae (Haliday) ash whitefly Punica granatum L. pomegranate Orange Total = 3 County Records Pest Scientific Name Drosophila suzukii (Matsumura) Drosophila suzukii (Matsumura) Drosophila suzukii (Matsumura) Drosophila suzukii (Matsumura) Drosophila suzukii (Matsumura) Pest Common Name Host Scientific Name Host Common Name County Drosophila N/A N/A Miami-Dade Drosophila N/A N/A Broward Drosophila Citrus sp citrus St Lucie Citrus x tangelo J. INGRAM & Drosophila H.E. MOORE tangelo Charlotte Drosophila Citrus sinensis (L.) OSBECK sweet orange Orange Raffaelea lauricola laurel wilt Persea borbonia (L.) redbay Levy Randia aculeata L. white indigoberry Selenaspidus articulatus (Morgan) rufous scale Monroe Xyleborus glabratus Eichhoff redbay ambrosia beetle Persea palustris (RAF.) SARG. swamp redbay Highlands Drosophila suzukii (Matsumura) Drosophila N/A N/A Martin Drosophila suzukii (Matsumura) Drosophila Eugenia uniflora L. surinam cherry Palm Beach Guignardia citricarpa citrus black spot Citrus sinensis (L.) Osbeck sweet orange Hendry Eriococcus quercus (Comstock) oak felt scale Quercus geminata SMALL sand live oak Palm Beach 3

Dactylopius confusus (Cockerell) Pseudococcus odermatti Miller & Williams Katacephala n. sp. Dysmicoccus brevipes (Cockerell) prickly pear cochineal scale Opuntia sp. prickly pear Hendry Sideroxylon celastrinum a mealybug (KUNTH) T.D. PENNINGTON saffron plum Pinellas Calyptranthes psyllid Calyptranthes pallens GRISEB. Spicewood Miami-Dade pineapple mealybug Cyperus sp. cyperus Miami-Dade Paramyiolia rhino (Steyskal) a tephritid fruit fly Citrus x paradisi MACFAD. grapefruit Brevard Pseudococcus longispinus (Targioni- longtailed Tozzetti) mealybug N/A N/A Taylor Euschistus quadrator Rolston a stink bug Tagetes sp. marigold Sarasota Total = 20 Host Records Pest Scientific Name Pest Common Name Host Scientific Name Host Common Name County Drosophila suzukii (Matsumura) Drosophila Fragaria x ananassa DUCHESNE strawberry Hillsborough Ceroplastes sinensis D. Guere Chinese wax scale Ficus aurea Nutt. Florida strangler fig Monroe Aulacaspis tubercularis (Newstead) armored scale Persea palustris (RAF.) SARG. swamp bay Miami-Dade Protopulvinaria pyriformis (Cockerell) a pyriform scale Pouteria campechiana (KUNTH) BAEHNI eggfruit tree (canistel) Broward Eucalymnatus tessellatus (Signoret) tessellated scale Pouteria campechiana (KUNTH) BAEHNI eggfruit tree (canistel) Broward Gynaikothrips uzeli Zimmerman weeping fig thrips Mikania micrantha Kunth mile-a-minute, bittervine, Chinese creeper Miami-Dade Icerya purchasi Maskell cottony cushion scale Batis maritima L. saltwort, turtleweed Pinellas Ceroplastes cirripediformis Comstock barnacle scale Clerodendrum quadriloculare (BLANCO) MERR. bronze-leaved clerodendrum Miami-Dade Coccus longulus (Douglas) long brown scale Sideroxylon celastrinum (KUNTH) T.D. PENNINGTON saffron plum Pinellas Eucalymnatus tessellatus (Signoret) tessellated scale Ixora parviflora VAHL torch tree Miami-Dade Pseudaonidia trilobitiformis (Green) trilobe scale Ixora parviflora VAHL torch tree Miami-Dade Pseudococcus odermatti Miller & Williams a mealybug Podocarpus gracilior PILG. weeping podocarpus; fern pine Miami-Dade Nipaecoccus viridis (Newstead) lebbeck mealybug Quercus myrtifolia WILLD. myrtle oak Palm Beach Asterolecanium pustulans (Cockerell) oleander pit scale Gossypium sp. cotton Monroe Parasaissetia nigra (Nietner) nigra scale Hibiscus furcellatus DESR lindenleaf rosemallow Palm Beach Pulvinaria acericola (Walsh & Riley) cottony mapleleaf scale Ilex vomitoria AIT. yaupon Alachua Coccus hesperidum (Linnaeus) brown soft scale Spondias dulcis G. FORST. otaheite, golden apple Broward Pinnaspis strachani (Cooley) lesser snow scale Hyophorbe lagenicaulis (L.H. BAILEY ) H.E. MOORE bottle palm, pignut palm Miami-Dade Nipaecoccus nipae (Maskell) coconut mealybug Lepidozamia peroffskyana REGEL a cycad Miami-Dade Pseudaulacaspis cockerelli (Cooley) magnolia white scale Hyphaene coriacea GAERTN. doum palm Miami-Dade 4

Nipaecoccus nipae (Maskell) coconut mealybug Thrinax brasiliensis MART. Brazilian thatch palm Miami-Dade Aonidiella taxus Leonardi asiatic red scale Podocarpus gracilior PILG. weeping podocarpus; fern pine Miami-Dade Eriococcus azaleae (Comstock) azalea bark scale Ceratiola ericoides MICHX. native rosemary Palm Beach Pseudococcus longispinus (Targioni- Tozzetti) longtailed mealybug Parthenocissus quinquefolia (L.) PLANCH. Virginia creeper St Lucie Brachycaudus helichrysi (Kaltenbach) leafcurl plum aphid Gnaphalium falcatum LAM. narrowleaf purple everlasting St Lucie Aleurodicus rugioperculatus Martin a whitefly Mangifera indica L. mango Miami-Dade Drosophila suzukii (Matsumura) Drosophila Eugenia uniflora L. surinam cherry Manatee Pseudococcus longispinus (Targioni- Tozzetti) Pseudococcus longispinus (Targioni- Tozzetti) Pseudococcus longispinus (Targioni- Tozzetti) Pseudococcus longispinus (Targioni- Tozzetti) Nipaecoccus nipae (Maskell) Eucalymnatus tessellatus (Signoret) longtailed mealybug Eugenia uniflora L. surinam cherry Miami-Dade longtailed mealybug Lolium perenne L. Italian ryegrass Miami-Dade longtailed Pentas lanceolata (FORSSK.) mealybug DEFLERS star flower Palm Beach longtailed mealybug Gardenia jasminoides gardenia Palm Beach Pseudophoenix vinifera coconut mealybug (MART.) BECC. wine palm Miami-Dade Manilkara zapota (L.) VAN tessellated scale ROYEN sapodilla Miami-Dade Aulacaspis tubercularis (Newstead) armored scale Manilkara zapota (L.) VAN ROYEN sapodilla Miami-Dade Toumeyella liriodendri (Gmelin) tuliptree scale Ilex cornuta LINDL. & PAXTON burford holly Alachua Coccus hesperidum (Linnaeus) brown soft scale Citrus hystrix DC. kaffir lime Miami-Dade Nipaecoccus viridis (Newstead) lebbeck mealybug Citrus sinensis (L.) Osbeck sweet orange, navel orange Palm Beach Planococcus citri (Risso) citrus mealybug Eugenia uniflora L. surinam cherry Miami-Dade Texas olive, Pulvinaria urbicola Cockerell urbicola soft scale Cordia boissieri A. DC. anacahuita Miami-Dade Milviscutulus mangiferae (Green) mango shield scale Rapanea punctata (LAM.) LUNDELL Florida rapanea Miami-Dade Zaprionus indianus Gupta a fruit fly Morus alba L. white mulberry St Lucie Drosophila suzukii (Matsumura) Drosophila Morus alba L. white mulberry St Lucie Aonidiella aurantii (Maskell) California red scale Olea europaea L. olive Orange Pouteria sapota (JACQ.) H. E. Coccus hesperidum (Linnaeus) brown soft scale MOORE & STEARN mamey sapote Orange Macrosiphum euphorbiae (Thomas) potato aphid Murraya paniculata (L.) JACK orange-jasmine Orange Aonidiella aurantii (Maskell) California red scale Punica granatum L. pomegranate Orange Hemiberlesia lataniae (Signoret) lantania scale Pimenta dioica (L.) MERR. allspice Orange Drechslera tritici-repentis leaf spotting pathogen Triticum aestivum wheat Columbia Liberobacter asiaticum citrus greening (Asian strain) Atalantia ceylanica ceylon atalantia Miami-Dade Drosophila suzukii (Matsumura) Drosophila Schoepfia chrysophylloides gulf graytwig St Lucie Crypticerya (Icerya) genistae Hempel a margarodid scale Desmodium incanum DC. zarzabacoa comun Palm Beach 5

Pseudoparlatoria ostreata Cockerell Saissetia coffeae (Walker) gray scale, armored scale hemispherical scale Acalypha wilkesiana MUELL. ARG. copperleaf, jacob's coat Broward Leucophyllum frutescens (BERL.) I.M. JOHNSTON cenizo, Texas-sage Broward Aleurodicus rugioperculatus Martin a whitefly Cocos nucifera L. coconut palm Miami-Dade Parasaissetia nigra (Nietner) nigra scale Calophyllum sp. kamani; beauty Leaf Miami-Dade Phenacoccus madeirensis Green a mealybug Poinsettia cyathophora (MURRAY) BARTL. wild poinsettia, fireon-the-mountain Palm Beach Mycetaspis personata (Comstock) masked scale Acca sellowiana (O. BERG) BURRETT pineapple guava Collier Total = 57 Table 1b. Historical pest records entered into NAPIS database. HISTORICAL U.S. Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name Katacephala n. sp. Calyptranthes psyllid Host Common Name County Year Calyptranthes pallens GRISEB. spicewood Broward 2003 Passionfruit woodiness virus (PWV) Total = 2 passionfruit woodiness virus (PWV) Passiflora choconiana a passionflower Broward 2009 HISTORICAL State Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name Total = 1 Host Common Name County Year Muell. Arg. croton Monroe 2008 HISTORICAL County Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name 6 Host Common Name County Year Raffaelea lauricola laurel wilt Persea borbonia (L.) redbay Highlands 2009 Muell. Arg. croton Broward 2008 Muell. Arg. croton Miami-Dade 2008 Muell. Arg. croton Palm Beach 2008 Muell. Arg. croton Lee 2008 Muell. Arg. croton Putnam 2008 Muell. Arg. croton St Lucie 2008 Muell. Arg. croton Duval 2008 Muell. Arg. croton Indian River 2008

Total = 16 Muell. Arg. croton Orange 2008 Muell. Arg. croton Brevard 2008 Muell. Arg. croton Seminole 2008 Muell. Arg. croton Hillsborough 2008 Muell. Arg. croton Collier 2008 Muell. Arg. croton Lake 2008 Muell. Arg. croton Hernando 2008 HISTORICAL Host Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name Host Common Name County Year N/A N/A N/A N/A N/A N/A Total = 0 Table 2a. Counties with new records for all categories sorted most to least, 2nd Quarter 2010. County # of Records County # of Records Miami-Dade 29 Brevard 1 Palm Beach 11 Charlotte 1 Orange 8 Columbia 1 Broward 7 Hamilton 1 St Lucie 6 Highlands 1 Monroe 4 Hillsborough 1 Pinellas 3 Levy 1 Alachua 2 Martin 1 Collier 2 Sarasota 1 Hendry 2 Taylor 1 Manatee 2 7 Total = 86 Table 2b. Counties with new historical records for all categories sorted most to least. County # of Records County # of Records Broward 3 Lee 1 Brevard 1 Miami-Dade 1 Collier 1 Monroe 1 Duval 1 Orange 1 Hernando 1 Palm Beach 1 Highlands 1 Putnam 1 Hillsborough 1 Seminole 1 Indian River 1 St Lucie 1 Lake 1 Total = 19

Table 3a. U.S./State records for plant pests only, 2nd Quarter 2010. U.S. Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name Host Common Name County Tospovirus Groundnut ringspot virus (GRSV) groundnut ringspot virus GRSV Lycopersicon esculentum tomato Miami-Dade Guignardia citricarpa citrus black spot Citrus sinensis (L.) Osbeck sweet orange Collier Paracoccus herreni Williams & Granara de Willink a mealybug Hibiscus rosa-sinensis L. Chinese Hibiscus Manatee Septoria mikania-micranthae septoria leaf spot of mikania Mikania micrantha Kunth bittervine, mile-aminute Miami-Dade Passionfruit mottle virus (PaMV) passionfruit mottle virus (PaMV) Passiflora sp. a passionflower Broward Xylosandrus amputatus Blandford an ambrosia beetle N/A N/A Orange Total = 6 State Plant Pest Records Pest Scientific Name Pest Common Name Host Scientific Name Host Common Name County Oxycarenus hyalinipennis (Costa) cotton seed bug Gossypium sp. wild cotton Monroe Hylocurus rudis (LeConte) an ambrosia beetle N/A N/A Hamilton Siphoninus phillyreae (Haliday) ash whitefly Punica granatum L. pomegranate Orange Total = 3 Table 3b. Historical U.S./State records for plant pests only. HISTORICAL U.S. Plant Pest Records Pest Scientific Name Katacephala n. sp. Passionfruit woodiness virus (PWV) Total = 2 Pest Common Name Calyptranthes psyllid Host Scientific Name Host Common Name County Year Calyptranthes pallens GRISEB. spicewood Broward 2003 passionfruit woodiness virus (PWV) Passiflora choconiana a passionflower Broward 2009 HISTORICAL State Plant Pest Records Pest Scientific Name Hodges and Hodgson Total = 1 Pest Common Name Host Scientific Name Host Common Name County Year Codiaeum variegatum (L.) Blume pictum var. (Lodd.) Muell. Arg. croton Monroe 2008 8

Table 4. State survey records entered into CAPS/NAPIS data base, 2 nd Quarter 2010. State Pest Survey Efforts Target Organism Scientific Name Target Organism Common Name Counties Surveyed # of Records Agrilus planipennis Fairmaire emerald ash borer 23 442 *1 Epiphyas postvittana (Walker) light brown apple moth (LBAM) 29 580 *2 Helicoverpa armigera (Hübner) see Soybean & SG Commodity Surveys N/A N/A Leptocybe invasa Fisher and LaSalle blue gum chalcid wasp (BGCW) N/A N/A Candidatus Liberibacter asiasticus citrus greening disease (Asian strain) 35 817 Lymantria dispar (L.) Asian gypsy moth (AGM) 0 0 Lymantria mathura (Moore) pink gypsy moth (PGM) 0 0 Monochamus,Trypodendron, Xylosandrus, bark beetle survey (SWPM) Hylurgops, Hylurgus 11 0 *3 Phytophthora ramorum Werres et al. sudden oak death (SOD) N/A N/A Puccinia horiana P. Henn. chrysanthemum white rust (CWR) N/A N/A Raffaelea lauricola Harrington & Fraedrich laurel wilt (LW) 10 0 *4 Raoiella indica Hirst red palm mite (RPM) N/A N/A Sirex noctilio F. bark beetle survey (SWPM / Sirex) 11 0 *5 Spodoptera littoralis Boisduval Egyptian cottonworm N/A N/A Spodoptera litura (F.) rice cutworm (RCW) 5 107 *6 Uromyces transversalis (Thum.) gladiolus rust (GR) N/A N/A redbay ambrosia beetle (SWPM / Xyleborus glabratus Eichhoff RBAB) 10 0 *7 Xylella fastidiosa Wells et al. citrus variegated chlorosis (CVC) N/A N/A Commodity Surveys - Potato potato cyst nematode survey (PCN) 1 0 *8 Commodity Surveys - Citrus citrus commodity survey (CCS) N/A N/A Commodity Surveys - Small Grains (SGS) Helicoverpa armigera, Spodoptera litura, Nysius huttoni, and Puccinia graminis tritici UG99 12 298 *9 Commodity Surveys - Oak see LBAM, AGM, and PGM above N/A N/A Total = 2,054 *1 EAB: 149 records represent trap checks from 136 traps in 26 counties plus screening of 33 negative EAB specimens, for the period of 12/1/2009 through 3/31/2010. (resulting in 2-NSR and 1-NCR this quarter, two of which were not considered pests and were not entered in NAPIS). Negative survey data entry in progress, ID ongoing. *2 LBAM: 683 records represent 750 negative moths (171 specimens submitted for analysis) from 117 traps in 29 counties (Alachua, Baker, Bradford, Brevard, Broward, Charlotte, Collier, Columbia, De Soto, Duval, Escambia, Hardee, Hernando, Highlands, Hillsborough, Indian River, Jackson, Lee, Leon, Manatee, Marion, Miami-Dade, Orange, Palm Beach, Pinellas, Polk, Sarasota, St. Lucie, Volusia). *3 SWPM/Bark Beetle: No records were uploaded this quarter, however survey trapping was ongoing in 33 traps in 11 counties (Bay, Brevard, Broward, Duval, Escambia, Hillsborough, Manatee, Miami-Dade, Nassau, Orange, Palm Beach) during this quarter, resulting in no new records or status change so negative data not input into NAPIS. All screenings and ID's to date are negative for the target species (totals are: 2384 specimens screened, 989 specimens identified). Negative survey data entry in progress, ID ongoing. *4 LW (part of RBAB Survey): No records were uploaded this quarter, however survey trapping was ongoing in 10 counties (Broward, Collier, Glades, Hendry, Highland, Lee, Martin, Miami-Dade, Orange, Palm Beach) during this period (see Redbay ambrosia beetle (RBAB) survey numbers below). For Miami-Dade County only, an intensive delimiting and trapping effort of about 100 traps that was initiated in March 2010 was scaled down to 51 traps and resulted in a total of 312 samples screened (approximately 3120 specimens) by the end of this Quarter. As of June 30, 2010, no additional X. glabratus beetles were found. *5 SWPM/Sirex: No records were uploaded this quarter, however survey trapping was ongoing in 33 traps in 11 counties (Bay, Brevard, Broward, Duval, Escambia, Hillsborough, Manatee, Miami-Dade, Nassau, Orange, Palm Beach) during 9

this quarter, resulting in no new records or status change so negative data not input into NAPIS. All screenings and ID's to date are negative for the target species (see notes for *3 above - SWPM/Bark Beetle - for screening information). *6 RCW: 107 records represent 173 negative moth samples from 21 traps, in 5 counties (DeSoto, Lake, Miami-Dade, Orange, Osceola) during April-May-June 2010. All screenings and ID's to date are negative for the target species. *7 RBAB: No records were uploaded this quarter, however survey trapping was ongoing in 87 traps (51 of the 87 are in Miami-Dade county) in 10 counties (Broward, Collier, Glades, Hendry, Highland, Lee, Martin, Miami-Dade, Orange, Palm Beach) during this quarter. All screenings and ID's to date are negative for the target species X. glabratus (totals for this quarter for all counties excluding Miami-Dade are: 1306 specimens screened, 365 specimens identified), and have resulted in 2-USA, 1-NCR and 1-NHR this quarter, only one of which was considered a pest and was entered in NAPIS. Negative survey data entry is in progress, ID ongoing. *8 PCN: No records were uploaded to NAPIS this quarter, however surveys were done on June 9-10, 2010 totaling 62 samples from 2100 acres in St. Johns County. Lab ID s and results are in progress. *9 SGS: 298 records represent 324 moths from 41 bucket traps for the target Helicoverpa armigera in 12 counties (Calhoun, Columbia, Escambia, Gadsden, Hardee, Hernando, Jackson, Okaloosa, Pasco, Polk, Santa Rosa, Sumner), covering the period 12-1-2009 thru 5-20-2010. Final recording of entries from this survey for Spodoptera litura, UG99 wheat rust, Nysius huttoni, and Cernuella virgata are in progress. All screenings and ID's are completed and are negative for all of the target species. Performance Measures : Surveys: Pest species, Host species, Counties, etc. Hours Acres Surveyed BGCW Survey: (Okeechobee/Seabring plantation survey) 1 Planning, 1 county Citrus Mite Survey w/dr Childers. 10 1 county, 104 sites, 100 acres Cotton Seed Bug Survey: Oxycarenus hyalinipennis; cotton; Monroe County; (6/14-6/18 Key West). 225 1 county, 39 sites Emerald Ash Borer Survey: Agrilus planipennis; Fraxinus spp.; Alachua, Baker, Bay, Citrus, Collier, DeSoto, Duval, Franklin, Glades, Gulf, Hamilton, Hardee, Hendry, Hernando, Highland, Hillsborough, Jackson, Lake, Lee, Levy, Liberty, Manatee, Marion, Martin, Nassau, Okeechobee, Orange, Pasco, Pinellas, Polk, Santa Rosa, Sarasota, St. Johns, Suwannee, Volusia, Wakulla, Walton counties. Early Detection/Rapid Response (EDRR) Project: wood-boring beetles; Alachua, Brevard, Duval, Hillsborough, Manatee, Orange, Pinellas and St. Lucie counties. 77 37 counties, 193 traps, multiple visits 41 8 counties, 36 traps, multiple visits Fruit Fly: survey homeowner concerns after ICS. 1.5 1 county Light Brown Apple Moth Survey: Epiphyas postvittana; many hosts; 81 29 counties, 117 traps, Alachua, Baker, Bradford, Brevard, Broward, Charlotte, Collier, multiple visits Columbia, De Soto, Duval, Escambia, Hardee, Hernando, Highlands, Hillsborough, Indian River, Jackson, Lee, Leon, Manatee, Miami-Dade Orange, Palm Beach, Pasco, Pinellas, Polk, Sarasota, St. Lucie and Volusia counties. Mile-a-Minute Survey: Mikania micrantha; Miami-Dade County 16 1 county, 27+ sites, multiple visits Nipaecoccus viridis Survey: Palm Beach County, June 8-10, 2010 107 1 county, 93 acres, 13 sites Potato Cyst Nematode (PCN) Survey: St. Johns County, June 9-10, 2010 27 1 county, (950 + 1150 acres) = 2100 acres Planococcus minor: many hosts; Miami-Dade County 1 1 site Redbay Ambrosia Beetle / Laurel Wilt Survey (LW/RBAB): Xyleborus 388.5 3 counties, 74 traps, glabratus and Raffaelea lauricola; Persea spp.: Miami-Dade, Broward, multiple visits 10

Palm Beach counties. Redbay Ambrosia Beetle Survey (RBAB): Xyleborus glabratus and Raffaelea lauricola; Persea spp.; Collier, Glades, Hendry, Highland, Lee, Martin, Orange counties. Rice Cutworm Survey: Spodoptera litura; many hosts; De Soto, Lake, Miami-Dade, Orange and Osceola counties. Small Grains Commodity Survey: Spodoptera litura, Helicoverpa armigera, Nysius huttoni, Ug99 rust; wheat, rye and oats; Calhoun, Columbia, Escambia, Gadsden, Hardee, Hernando, Jackson, Okaloosa, Pasco, Polk, Santa Rosa and Sumter counties SWPM Survey: Sirex noctillio and exotic wood boring beetles; pine and other trees; Bay, Brevard, Broward, Duval, Escambia, Hillsborough, Manatee, Miami-Dade, Nassau, Orange, Palm Beach Counties 36 7 counties, 13 traps, multiple visits 33 5 counties, 21 traps, multiple visits 149 12 counties, 75 traps, 35 sites (includes bucket, sweep, beat, UV, Pitfall), 5,400 acres, multiple visits 0 11 counties, 33 traps, multiple visits, see hours under Pest Identification 1194 Conferences/Trainings/Workshops Hours Training Provided by: Bark Boring Insect Workshop 24 IFAS CAPS Monthly Conference Calls 11 N/A Miami-Dade Natural Areas Management: New Management issues in 3 CAPS Miami Dade for 2010 on April 16th, 2010. Southern Plant Board Meeting, Montgomery AL (4/25-4/28) 32 N/A NFPRC conference call, June 8, 2010 1.5 Cotton Seed Bug conference call with DEP personnel for Fort Zach. 1 Taylor Historical State Park LBAM Conference call in CAPS director office June 10, 2010 1 Laurel Wilt Working Group on April 15th, 2010 in Fort Pierce, Florida. 4 USDA,,FDACS, IFAS Red bay Ambrosia beetle/laurel wilt workgroup meeting 10 April 15, Fort Pierce FL. Training - Outlook web-x seminar 2 FDACS 89.5 Projects Hours CAPS Web Page 4 Downloadable Calendars 6 CAPS 1 st Quarter Report (includes development, editing and printing) 40.5 Miscellaneous Reports (4 survey reports + 3 special reports + reviewing) 35 Miscellaneous Accomplishment Reports (Quarter, LBAM, EAB, PCN) 6 Miscellaneous Artwork (3 report covers, 3 certificates) 10 Miscellaneous Artwork (graphics, photos, CSB Host Handout) 6 Outreach (Open Lines for LW, J. Kummer for surveys) 2 CHRP 10 Mapping Farm Bill - meetings and calls 8 Grapevine Moth meetings and calls 2 Plum pox virus (PPV) (Farm Bill) Prepare work plan for PPV 14 Red Palm Mite 10 Mapping Tuta absoluta meetings and calls 1 White Fly meetings and calls 5 Zebra chip disease meetings and calls 2 NAPIS Citrus Greening/HLB 7 Data collection, 39 counties 11

NAPIS--EAB 42 N/A NAPIS--LBAM 23 N/A NAPIS--RBAB 15 N/A NAPIS--Small Grains 18 N/A NAPIS--Spodoptera 10 N/A NAPIS--SWPM 7 N/A 283.5 Pest Identification (screening, sorting, identification, etc.) Hours No. of specimens examined Emerald Ash Borer sample processing and identification 6 Screened 31 samples (76 specimens), 37 identified LW-RBAB Survey (Miami-Dade only) 120 Screened 312 samples, (approx. 3120 specimens) Redbay Ambrosia Beetle sample processing and identification 85 1306 specimens, 365 identified Solid Wood Packing Material sample processing and identification 160 2384 specimens, 989 identified Synoptic collection of Florida s Scolytidae, Platypodidae, Buprestidae 20 N/A and Cerambycidae (for CAPS) (ongoing) Worked in FSCA 88 N/A 479 Presentations Public Outreach Hours No. of People in Audience 2010 Miami ARS Dedication 4 all ARS 2010 Miami ARS Community Outreach Day 4 all ARS Laurel Wilt and the Redbay Ambrosia Beetle in Florida: status update to 0.5 22 the Laurel Wilt Working Group on April 15th, 2010; Fort Pierce, Florida. A New invasive weed: Creeping Bittervine, Mikania micrantha Kunth to Miami-Dade Natural Areas Management on April 16th, 2010; Miami, Florida. 1.5 10 10 Other Pertinent Activities Hours Backup and Organize I-drive 67 NAPIS ongoing data entry (CG, NSR, NCR, etc) 66 ISIS - data entry (EAB) 2 Purchasing misc 4 Truck maintenance 2 N/A Pack and move office into temporary storage after temporary office has been removed from ARS campus while waiting for new office to be opened. 30 N/A subtotal this section: 171 Total all sections: 2227 US-State-County-Host Records (Species) Record Date Oxycarenus hyalinipennis E2010-1498-1 ( Monroe) USA 20100323 - CAPS Survey - W Thiel Septoria mikania-micranthae PPST-2010-33451 ( Miami-Dade) USA 20100114 - MM Survey - DG-EH 12

Xylosandrus amputatus E2010--USA-- ( Orange) USA 20100518 - EDRR Survey - BS-MW Hylocurus rudis E2010-1874-1 ( Hamilton) NSR 20091125 - EAB Survey - LW Eriococcus quercus E2010-0598-1 ( Palm Beach) NCR 20100204 - CAPS Survey - AD-KG Randia aculeata L. E2010-1655-1 ( Monroe) NCR 20100401 - CSB Survey - AD-KG Xyleborus glabratus E2010-1952-1 ( Highlands) NCR 20100415 - CAPS Survey - DG-MLPayne Homaemus proteus E2010-3449-1 ( Palm Beach) NCR 20100609 - NViridis Survey - AD-KG-DPI Raffaelea lauricola PPST-2010-30012 ( Highlands) NCR 20091021 - RBAB Survey - DG Eriococcus azaleae E2010-0596-1 ( Palm Beach) NHR 20100204 - CAPS Survey - AD-KG Nipaecoccus viridis E2010-1126-- ( Palm Beach) NHR 20100304 - CAPS Survey - AD-KG Asterolecanium pustulans E2010-1664-1 ( Monroe) NHR 20100331 - CSB Survey - AD-KG Parasaissetia nigra E2010-1920-2 ( Palm Beach) NHR 20100413 - CAPS Survey - AD-KG Ceroplastes sinensis E2010-1658-2 ( Monroe) NHR 20100331 - CSB Survey - AD-KG Aulacaspis tubercularis E2010-2064-2 ( Miami-Dade) NHR 20100420 - RBAB Survey - AD-KG Pseudococcus longispinus E2010-3167-1 ( Palm Beach) NHR 20100526 - NViridis Survey - AD-KG Pseudococcus longispinus E2010-3171-2 ( Palm Beach) NHR 20100526 - NViridis Survey - AD-KG Drechslera tritici-repentis PPST-2010-35871 ( Columbia) NHR 20100413 - SG Survey - LW Euceropsylla martorelli E2010-2229-2 ( Miami-Dade) USA (*) 20100502 - CAPS Survey - JB Cryptolestes atratulus E2010-2187-1 ( Miami-Dade) USA (*) 20100407 - LW-RBAB Survey - AD-JYoung Strongylium cultellatum E2010-3375-1 ( Miami-Dade) USA (*) 20100525 - LW-RBAB Survey - AD-KG Hylocurus binodatus E2010-1873-1 ( Hamilton) NSR (*) 20091125 - EAB Survey - LW Chrysobothris scitula E2010-2624-5 ( Alachua) NSR (*) 20100510 - CAPS Survey - AS-DS Agalliopsis cervina E2010-2256-1 ( Glades) NCR (*) 20100427 - EAB Survey - M.Terrell Oxoplatypus quadridentatus E2010-2679-1 ( Highlands) NCR (*) 20100330 - RBAB Survey - D.Harris Camillina pulcher E2010-3676-3 ( Miami-Dade) NCR (*) 20100330 - CAPS Survey - JB (*) denotes non-pest, therefore not entered in NAPIS 26 Records FDACS-DPI-CAPS and USDA-PPQ-CAPS Personnel: Dr. Leroy Whilby, State Survey Coordinator, whilbyl@doacs.state.fl.us Dr. Edward Hung, Pest Survey Specialist, hungs@doacs.state.fl.us Andrew Derksen, Pest Survey Specialist, derksea@doacs.state.fl.us 13

Cynthia Moncrief, Public Information Specialist & NAPIS data manager, moncric@doacs.state.fl.us David Saeger, GIS/Mapping Specialist, saegerd@doacs.state.fl.us Kate Okins, Laboratory Technician, okinsk@doacs.state.fl.us Dr. Wayne Dixon, CAPS Program Manager; Assistant Director, DPI, dixonw@doacs.state.fl.us Paul Hornby, State Plant Health Director, paul.l.hornby@aphis.usda.gov Ann E. Wildman, Assistant State Plant Health Director, Ann.E.Wildman@aphis.usda.gov Dr. Catherine Marzolf, Florida State Operation Support Officer (SOSO), Catherine.A.Marzolf@aphis.usda.gov Julieta Brambila, Entomologist Identifier, Julieta.Brambila@aphis.usda.gov Dr. Adam Silagyi, Pest Survey Specialist, Adam.J.Silagyi@aphis.usda.gov Brian Saunders, Pest Survey Specialist, brian.d.saunders@aphis.usda.gov Doug Restom-Gaskill, Pest Survey Specialist, douglas.a.restomgaskill@aphis.usda.gov Michael Weiss, Agricultural Technician, michael.weiss@aphis.usda.gov Karolynne Griffiths, Pest Survey Specialist, karolynne.m.griffiths@aphis.usda.gov Photograph Credits: Cover page: Background photo Small Grains Survey in Florida s Panhandle, by Leroy Whilby, FDACS-CAPS. Photographs (left to right) courtesy of: LEFT PANEL: Small Grains Survey in Florida s Panhandle, by Leroy Whilby, FDACS-CAPS; Inset (top): Helicoverpa armigera, by Merle Shepard, Gerald R. Carner, and P.A.C. Ooi; UGA5368054; Inset (bottom): Spodoptera litura, by K. Kiritani, Japan; www-eppo-org; MIDDLE PANEL: Florida State Record: Hylocurus rudis, SEM photo by Tom Atkinson, Pest Management Research; UGA5173049; County and Host Record: Pseudococcus longispinus, by David Cappaert, Michigan State University; UGA2131069; U.S. Record: Guignardia citricarpa (citrus black spot) on valencia orange, by P. Barkley, Biological and Chemical Research Institute; UGA0746013; U.S. Record: Xylosandrus amputatus, by Kate Okins, FDACS-DPI; RIGHT PANEL: U.S. Record: Guignardia citricarpa on citrus, by Cesar Calderon, USDA-PPQ; UGA2171092; Florida State Record: Siphoninus phillyreae, by James K. Lindsey; Wikimedia Commons. Interior pages: Maps by David Saeger, FDACS-DPI-CAPS, and Matt Albritton, FDACS-DPI. Photographs courtesy of: Cotton Seed Bug Survey, by Karolynne Griffiths, USDA-APHIS-PPQ; Downloadable calendar, design by Cyndi Moncrief, FDACS-DPI-CAPS; EDRR Survey, by Cynthia Moncrief, FDACS-DPI-CAPS. Prepared By: Dr. Leroy Whilby, FDACS-DPI-CAPS Cynthia Moncrief, FDACS-DPI-CAPS Dr. Wayne Dixon, FDACS-DPI and CAPS 14

Maps: 15

Map 1. Redbay Ambrosia Beetle and Laurel Wilt Survey in Miami-Dade County. Map 2. Cotton Seed Bug (Oxycarenus hyalinipennis) Survey in Monroe County, showing the positive location and the sentinel sites. 16

Figures: Fig. 1. Andrew Derksen (FDACS-DPI-CAPS) checking a large cotton plant at a residence during the Cotton Seed Bug Survey in Monroe County. Inset shows a close-up of cotton seed bugs in the cotton boll. 17

Fig. 2. CAPS downloadable calendar, May 2010. 18

Fig. 3. Leroy Whilby (FDACS-DPI-CAPS) checking a lindgren funnel trap during the Florida EDRR Survey in the Alachua County area. Inset photo shows filtering of cup contents for insect collection. 19