l?\ DEVELOPMENT OF CARBONATED HERBAL NELLI DRINK 1~~9647 Kushan Chanaka Amarasinghe p.,101)..'\

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~-- ----------.-... p.,101)..'\ l?\ 0\' rj;) o DEVELOPMENT OF CARBONATED HERBAL NELLI DRINK (Phyllanthus emblica.) By Kushan Chanaka Amarasinghe Llbary - USJP 1111I11111 111111 199647 B.Sc. (Sp.) in Food Science and Technology 2010 1~~9647

ABSTRACT Amla fruit is considered as the mother of Ayurvedic medicine and there is a wide array of pharmaceutical and food products manufactured using fresh Amla fruit, pulp and Amla fruit powder. Amla fruit cordial which is commonly found in south india and upper of part of Sri lanka is well known as Nelli cordial. Due to appealing demand in the market for healthy fruit drinkswith carbonation, this study was undertaken to develop a product which has both natural nd artificialattributes of a standard soft drink. This study attempted to identify a suitable recipe to develop a Carbonated Amla fruit drink (Nellidrink) by using fruit extraction (pulp) and sugar syrup. The study also examined for a period of 8 weeks the variation of chemical and microbiological changes in the product such as PH, Microbiology count, brix value and carbonation in an attempt to identify the shelf life of the product. The results showed that for a period of 8 weeks of those parameters were withinthe accepted range as prescribed by SLS soft drinks regulations. Extraction of Amla fruit juice was done using pulping method and adding constant ratio of water to amla fruit pieces and obtained extraction was mixed with sugar syrup which has different brix values. Amla fruit extraction and sugar syrup was mixed to formulate the flavoured syrup (1000ml) which has a higher brix value (21.1) and then 100 ml from the r flavoured syrup was mixed with 100 ml of carbonated water (4v/v) where the final drink bottle of 200ml posses 2v/v amount of carbonation. Optimum Amla fruit extraction for making of 1000ml was 200ml and 800ml from sugar syrup. Final brix value of the product was 11 as obtained from sensory evaluations and benzoic acid was used as the preservative and citric acid was also used to enhance flavour (0.003%). Final PH of the product was set to 3.3. As storing conditions both room temperature and cool room temperature (4 C) was used. Vll

Contents CHAPTER 1 5 1.1 Introduction 5 1.2 Objectives 6 1.2.1 Location 6 CHAPTER2 7 2.1 Background 7 2.2 Plant Description 8 2.3 The common names 9 2.4 Habitat 9 2.5 The Ayurvedic description of Amla 10 2.6 Chemical constituents 10 2.6.1 Composition of the Amla fruit pulp 12 2.7 Preparations 12 2.8 Description of the plant. 12 2.8.1 Parts used 12 2.9 Amla fruit varieties 13 2.9.1 Commercial varieties 13 2.9.2 Recommended species... 13 2.10 Growing conditions for Phyllanthus emblica sp 15 2.10.1Soil. 15 2.10.2 Cultivation 15 2.10.3 Fertilizing 15 2.10.4 Prevention of Boron deficiency 16 2.11 Uses of Amla 16 2.11.1 Aperient 16 2.11.2 Antibacterial, antifungal, antiviral.. 16 2.11.3 Antioxidant 16 2.11.4 Aphrodisiac, 16 2.11.5 Boils and spots 17 2.11.6 Chelating agent 17 2.11.7 Constipation 17 2.11.8 Dental problems 17 1

2.11.9 Diabetes 17 2.11.10 Diarrhoea 18 2.11.11 Diuretic 18 2.11.12 Fevers 18 2.11.13 Gonorrhoea 18 2.11.14 Hair growth 19 2.11.15 Headache 19 2.11.16 Indigestion 19 2.11.17 Inflammation 19 2.11.18 Mouth ulcers 19 2.11.19 Nausea 20 2.11.20 Nose bleed 20 2.11.21 Perfumery 20 2.11.22 Pruritis 20 2.11.23 Respiratory problems 20 2.11.24 Scurvy 21 2.11.25 Skin sores and wounds 21 2.11.26 Skin whitening 21 2.11.27 Sore eyes 21 r 2.11.28 Tonic 21 2.11.29 Vaginal complaints 22 2.11.30 Water purification 22 2.11.31 Worms 22 2.11.31 Miscellaneous 22 2.12 Preservation 22 2.13 Products in the market based on Amla source 22 CHAPTER 3 24 3.1 Materials & Methods 24 3.1.1. Idea generation (Step 1) 24 3.1.2 Idea Screening (Step 2) 25 3.1.3 Concept development and testing (Step 3) 25 3.1.4 Product Development (Prototype) (Step 4) 26 3.2 Methodology 26 3.2.1 Work Plan 26 2

3.2.2 Materials & Equipmerits 27 3.2.3 Ingredients 28 3.2.5 Other ingredients 30 3.3 Processing technology of Nelli Krush (Amla fruit drink) 31 3.3.1 Preparation of fresh amla fruit extraction 31 3.3.2 Preparation of sugar syrup 32 3.3.3. Preparation of citric acid solution 34 3.3.4 Preparation of sodium benzoate solution 35 3.4 Development technology of flavoured syrup 36 3.4.1 Selection of Optimum Amla fruit extraction volume for the product.. 36 3.4.2 Selection of best Brix value (measured sensory parameters are sweetness & overall acceptability) 37 3.4.3 Preparation of 800ml stock syrup 38 3.5 Selection of the best carbonation level of the drink 39 3.5.1 Method 39 3.6 Preparation of final drink using selected value '" '" 40 3.6.1 Materials 40 3.6.2 Method 40 3.7 Process flow diagram 42 r 3.8 Labeling 43 3.9 Sensory Evaluation 43 3.10 Analysis of the quality parameters of the final product...44 3.10.1 Laboratory analysis 44 3.10.2 Proximate Analysis 47 3.10.3 Microbiological Examinations 50 CHAPTER 4 52 4.1 Results and Discussion 52 Development technology of Carbonated Nelli drink 52 4.1.1.Quality of the raw material.. 52 4.1.2 Determination of best Amla extraction ratio for the product 53 4.1.3 Amla Concentration versus Treatment blocked by Observation 54 4.1.3.2 Paired comparison for Amla Concentration 55 4.1.4 Determination of the best brix level. 56 4.1.5 Determination of the best carbonation level.. 58 3

4.1.6 Preparation of final product using selected value 60 4.1.7 Shelflife study of the Nelli krush 64 4.1.8 Physiochemical analysis of final product.. 73 CHAPTER 5 78 5.1 Conclusion and Recommendation 78 5.1.1 Conclusion 78 5.1.2 Recommendations 79 LIST OF REFERENCES ~ 80 References 80 4