Objective. SROC Calf and Heifer Research Facility. Data for study

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Relationships between protein and energy consumed from milk replacer and starter and calf growth and first lactation production performance of Holstein dairy cows J. Rauba 1, B.J. Heins 2, H. Chester-Jones 3, D. Ziegler 3, Jim Linn 1, N. Broadwater 4 1 Milk Specialties Global, 2 University of Minnesota West Central Research and Outreach Center, Morris, MN, 3 University of Minnesota Southern Research and Outreach Center (SROC),Waseca, MN, 4 University of Minnesota Extension Service Emeritus, Rochester, MN. Objective Determine the relationships between protein and energy (ME) consumed from milk replacer and starter and first lactation performance of Holstein dairy cows Merging data from 45 studies across 10 years for a multiple study analysis should improve our understanding of the effects of early life protein and energy consumed and first-lactation performance SROC Calf and Heifer Research Facility Heifer calves from 3 commercial dairies Data for study Data were collected from birth year of 2004 to 2014 for 4,534 Holstein animals Lactation data was analyzed for 3,627 animals. Lactation data was from 2006 to 2017 Data used in this study was from the first 60 days of life. Calves remained at SROC for another 3 to 4 months and then either returned to their home farm or a commercial grower.

Data for Study, continued Body weights (BW) were taken on arrival and d 14, 28, 42, 49, 56 and when leaving SROC Health treatments were recorded for each calf Data for Study, continued Starter and water were offered in open pails. Amounts of milk replacer and starter offered and refused were recorded daily Health treatments were recorded for each calf Data for Study, continued All farms on DHIA (DRMS, Raleigh NC) First lactation data collected from monthly tests Actual 305 d milk Milk protein and fat yield and concentrations Calf data analysis Milk Replacers 20 to 28% Protein, majority were 20-20 milk replacer Starter was a commercial texturized starter Majority were 18% Protein, but varied from 18 to 22% for a few studies ME of Milk Replacer and Starter were calculated using NRC formulas Calf protein and ME requirements were from the 2001 Dairy NRC

Calf Data Analysis, continued ADG Class at 8 wk for Milk Replacer and Starter Protein Intake (lb) Formulas (NRC, 2001): ME (Mcal/kg) = 0.1 LW 0.75 + (0.84 LW 0.355 x LWG 1.2 ) ADG (lb/d) 0.50 0.75 0.75 1.0 1.0 1.25 1.25 1.50 1.50 1.75 >1.75 Accumulative protein intake (lb) over 8 wk, LS Means ME Content of MR: ME (Mcal/kg) = [0.057 CP (%) + 0.092 Fat (%) + 0.0395 Lactose (%)] 0.9312 Milk Replacer Protein Starter Protein 11.02 a 10.41 bc 10.32 c 10.41 bc 10.45 b 10.90 a 6.90 f 11.53 e 16.18 d 19.97 c 23.85 b 28.64 b Combined Protein 23.15 f 31.81 e 42.50 d 51.30 c 60.00 b 71.10 a abc Values in the same row with different superscripts are different (P < 0.05) 80 70 Effect of Protein Intake on ADG Milk Replacer Starter Total ADG Class at 8 wk for Milk Replacer and Starter ME Intake (Mcal/lb) ADG (lb/d) 0.50 0.75 0.75 1.00 1.00 1.25 1.25 1.50 1.50 1.75 >1.75 60 Protein Intake, total lb 50 40 30 20 Fed ad lib Accumulative ME Intake (Mcal/lb) over 8 wk, LS Means Milk Replacer ME 238.45 a 225.95 c 223.9 c 224.61 c 225.22 c 231.59 b 10 Limit fed Starter ME 109.02 f 184.03 e 258.16 d 318.85 c 380.44 b 455.1 a 0 0.5 0.75 0.75 1.00 1.00 1.25 1.25 1.50 1.50 1.75 >1.75 Average Daily Gain, lb/day Combined ME 250.04 f 274.89 e 311.56 d 343.41 c 377.50 b 426.20 a abc Values in the same row with different superscripts are different (P < 0.05)

500 450 Effect of ME Intake on ADG Milk Replacer Starter Total Calf Milk Replacer and Starter Protein Intake (lb) at 8 wk to predict first-lactation 305-d milk yield ME Intake, total Mcal/lb 400 350 300 250 200 150 Starter Fed ad lib MR Limit fed Milk Replacer Protein (lb) Starter Protein (lb) Combined Protein (lb) Variable Week Estimate P value Estimate P value Estimate P value 305 d milk 8 76.3 0.27 50.5 0.06 24.96 0.03 100 305 d fat 8 3.09 0.34 2.89 0.004 1.19 0.006 50 0 0.5 0.75 0.75 1.00 1.00 1.25 1.25 1.50 1.50 1.75 >1.75 Average Daily Gain, lb/day 305 d protein 8 1.52 0.48 2.91 <0.001 1.30 <0.001 Calf Milk Replacer and Starter ME Intake (Mcal/lb) at 8 wk to predict first-lactation 305-d milk yield Effect of birth season on 8 wk milk replacer and starter protein intake (lb), and milk replacer and starter ME (Mcal/lb) Spring Summer Fall Winter Milk Replacer ME (Mcal/lb) Starter ME (Mcal/lb) Combined ME (Mcal/lb) Protein Intake, lb Milk Replacer 10.71 ab 10.69 ab 10.52 b 10.82 a Variable Week Estimate P value Estimate P value Estimate P value 305 d milk 8 8.00 0.12 3.15 0.06 3.97 0.02 305 d fat 8 0.33 0.14 0.18 0.01 0.20 0.001 305 d protein 8 0.20 0.20 0.18 <0.001 0.20 <0.001 Starter 15.28 c 15.56 c 16.40 b 17.06 a Combined 47.72 d 49.29 c 51.17 b 51.4 a ME Intake, Mcal/lb Milk Replacer 231.00 b 229.94 bc 227.71 c 233.91 a Starter 243.32 c 247.66 c 261.51 b 271.89 a Combined 528.09 c 533.18 c 543.85 b 551.15 a ab Values in the same row with different superscripts are different (P < 0.05)

Summary Increasing intake of Protein and ME during the first 8 weeks of life increased ADG Although Protein and ME of Milk Replacer was a significant contributor to ADG in these studies, Starter Protein and ME had the largest effect due to ad lib feeding Consuming higher amounts of both Protein and ME during the first 8 weeks of life positively affected first lactation 305 day milk Summary, continued Variation was high in predicting first lactation milk production from Protein and ME intake in early life, indicating additional factors affect first lactation milk production One factor shown in these studies was season of birth with calves born in the fall and winter have greater combined Protein and ME intake Thank you Jrauba@milkspecialties.com 952-942-7310 x 5164